Journal: BMC Biotechnology

Article Title: Evaluation of five different cDNA labeling methods for microarrays using spike controls

doi: 10.1186/1472-6750-3-23

Figure Lengend Snippet: Accuracy and reproducibility of five different labeling methods in cDNA microarray hybridizations 1 .

Article Snippet: For the CyScribe, FairPlay and TSA Micromax methods, the hybridization-mixture was slightly modified compared to manufacturer's recommended procedure: 7.9 μl 20x SSC, 1.4 μl 10% SDS, 2.5 μl 50x Denhardt, 1 μl yeast tRNA (4 μg/μl), 1 μl poly d(A) (6 μg/μl) and 2 μl 1% BSA were added to the labeled and purified cDNA.

Techniques: Labeling, Microarray