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Neogen trypticase soy agar
Ex vivo Listeria ( L. ) monocytogenes clearance by macrophages and examination of livers from exendin-4 treated db/db mice. (A) CFU values of supernatants of DMEM contained Listeria -infected macrophages were determined using the plate method with <t>trypticase</t> soy agar (TSA) plate. (B) The number of L. monocytogene CFUs among centrifuged pellets of macrophages were lysed in sterile cold water and determined using the plate method with TSA plate. Results are expressed as the mean ± SD. Statistically significant differences between control (mock) and exendin-4 treated db/db mice are indicated by an asterisk ( * p
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Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice

Journal: Journal of Veterinary Science

doi: 10.4142/jvs.2012.13.3.245

Ex vivo Listeria ( L. ) monocytogenes clearance by macrophages and examination of livers from exendin-4 treated db/db mice. (A) CFU values of supernatants of DMEM contained Listeria -infected macrophages were determined using the plate method with trypticase soy agar (TSA) plate. (B) The number of L. monocytogene CFUs among centrifuged pellets of macrophages were lysed in sterile cold water and determined using the plate method with TSA plate. Results are expressed as the mean ± SD. Statistically significant differences between control (mock) and exendin-4 treated db/db mice are indicated by an asterisk ( * p
Figure Legend Snippet: Ex vivo Listeria ( L. ) monocytogenes clearance by macrophages and examination of livers from exendin-4 treated db/db mice. (A) CFU values of supernatants of DMEM contained Listeria -infected macrophages were determined using the plate method with trypticase soy agar (TSA) plate. (B) The number of L. monocytogene CFUs among centrifuged pellets of macrophages were lysed in sterile cold water and determined using the plate method with TSA plate. Results are expressed as the mean ± SD. Statistically significant differences between control (mock) and exendin-4 treated db/db mice are indicated by an asterisk ( * p

Techniques Used: Ex Vivo, Mouse Assay, Infection

Related Articles

Mouse Assay:

Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Article Snippet: Adherent PECs (2 × 105 cells) collected from db/db mice treated with or without exendin-4 were incubated with L. monocytogenes [5 × 106 colony forming units (CFUs)] in DMEM without antibiotics for 30 min at 37℃. .. The numbers of intracellular and extracellular bacteria were analyzed by performing a CFU assay with trypticase soy agar (Neogen, USA).

Incubation:

Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Article Snippet: Adherent PECs (2 × 105 cells) collected from db/db mice treated with or without exendin-4 were incubated with L. monocytogenes [5 × 106 colony forming units (CFUs)] in DMEM without antibiotics for 30 min at 37℃. .. The numbers of intracellular and extracellular bacteria were analyzed by performing a CFU assay with trypticase soy agar (Neogen, USA).

Colony-forming Unit Assay:

Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Article Snippet: .. The numbers of intracellular and extracellular bacteria were analyzed by performing a CFU assay with trypticase soy agar (Neogen, USA). .. Listeria challenge and pathological examination For the protection study of exendin-4 on Listeria -infected diabetic mice, db/db mice were injected twice daily with PBS or exendin-4 (10 µg/kg body weight) from 6 weeks to 10 or 20 weeks of age.

Activity Assay:

Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Article Snippet: Paragraph title: Phagocytic assay, Listeria detection in macrophages, and bacteria phagocytic activity ... The numbers of intracellular and extracellular bacteria were analyzed by performing a CFU assay with trypticase soy agar (Neogen, USA).

Modification:

Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Article Snippet: Dextran 40-FITC (1 mg/mL; Sigma, USA) was added to Dulbecco's modified Eagle's medium (DMEM) (GIBCO, USA) for 30~60 min. PECs were washed twice with FACS buffer (PBS supplemented with 0.1% FBS) and once with FACS fixative buffer (2% paraformaldehyde in FACS buffer), and analyzed with FACS. .. The numbers of intracellular and extracellular bacteria were analyzed by performing a CFU assay with trypticase soy agar (Neogen, USA).

FACS:

Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Article Snippet: Dextran 40-FITC (1 mg/mL; Sigma, USA) was added to Dulbecco's modified Eagle's medium (DMEM) (GIBCO, USA) for 30~60 min. PECs were washed twice with FACS buffer (PBS supplemented with 0.1% FBS) and once with FACS fixative buffer (2% paraformaldehyde in FACS buffer), and analyzed with FACS. .. The numbers of intracellular and extracellular bacteria were analyzed by performing a CFU assay with trypticase soy agar (Neogen, USA).

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    Neogen trypticase soy agar
    Ex vivo Listeria ( L. ) monocytogenes clearance by macrophages and examination of livers from exendin-4 treated db/db mice. (A) CFU values of supernatants of DMEM contained Listeria -infected macrophages were determined using the plate method with <t>trypticase</t> soy agar (TSA) plate. (B) The number of L. monocytogene CFUs among centrifuged pellets of macrophages were lysed in sterile cold water and determined using the plate method with TSA plate. Results are expressed as the mean ± SD. Statistically significant differences between control (mock) and exendin-4 treated db/db mice are indicated by an asterisk ( * p
    Trypticase Soy Agar, supplied by Neogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Neogen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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    Ex vivo Listeria ( L. ) monocytogenes clearance by macrophages and examination of livers from exendin-4 treated db/db mice. (A) CFU values of supernatants of DMEM contained Listeria -infected macrophages were determined using the plate method with trypticase soy agar (TSA) plate. (B) The number of L. monocytogene CFUs among centrifuged pellets of macrophages were lysed in sterile cold water and determined using the plate method with TSA plate. Results are expressed as the mean ± SD. Statistically significant differences between control (mock) and exendin-4 treated db/db mice are indicated by an asterisk ( * p

    Journal: Journal of Veterinary Science

    Article Title: Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice

    doi: 10.4142/jvs.2012.13.3.245

    Figure Lengend Snippet: Ex vivo Listeria ( L. ) monocytogenes clearance by macrophages and examination of livers from exendin-4 treated db/db mice. (A) CFU values of supernatants of DMEM contained Listeria -infected macrophages were determined using the plate method with trypticase soy agar (TSA) plate. (B) The number of L. monocytogene CFUs among centrifuged pellets of macrophages were lysed in sterile cold water and determined using the plate method with TSA plate. Results are expressed as the mean ± SD. Statistically significant differences between control (mock) and exendin-4 treated db/db mice are indicated by an asterisk ( * p

    Article Snippet: The numbers of intracellular and extracellular bacteria were analyzed by performing a CFU assay with trypticase soy agar (Neogen, USA).

    Techniques: Ex Vivo, Mouse Assay, Infection