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Difco trypticase soy agar
Trypticase Soy Agar, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy agar/product/Difco
Average 94 stars, based on 21 article reviews
Price from $9.99 to $1999.99
trypticase soy agar - by Bioz Stars, 2020-04
94/100 stars

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High Performance Liquid Chromatography:

Article Title: Identification and Characterization of a Novel Terrabacter ginsenosidimutans sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ †
Article Snippet: Growth on nutrient agar and Trypticase soy agar (Difco, Detroit, MI) was also evaluated at 30°C. .. The crude n -hexane-quinone solution was purified using silica Sep-Pak Vac cartridges (Waters) and subsequently analyzed by HPLC, as previously described by Collins ( ).

DNA Extraction:

Article Title: Improved method for rapid and accurate isolation and identification of Streptococcus mutans and Streptococcus sobrinus from human plaque samples
Article Snippet: .. During the 48 h incubation of the MS ID plates (at 24 h or when sufficient colony growth was observed), plates were removed briefly from the incubator and a single colony from each MS ID plate was streaked onto Tryptic Soy Broth agar with 0.5% yeast extract (TSB-YE) (Difco, Sparks, MD, USA) to be used for rapid DNA extraction and freezer stocks of each isolate. .. TSB-YE plates were streaked from the SMAB side of the MS ID plates due to more rapid growth and more butyrous colonies.

Centrifugation:

Article Title: Actinobacillus actinomycetemcomitans Serotype d-Specific Antigen Contains the O Antigen of Lipopolysaccharide
Article Snippet: Briefly, the strains were grown on Trypticase soy agar (Difco Laboratories, Detroit, Mich.) plates in 5% CO2 in air at 37°C for 2 to 3 days. .. The total bacterial cell membrane fraction was recovered by centrifugation at 100,000 × g for 1 h and treated with Sarkosyl (sodium lauryl sarcosinate) (Sigma Chemical Co., St. Louis, Mo.) at room temperature for 1 h, and the solution was centrifuged at 100,000 × g for 1 h. The pellet was suspended in distilled water, dispersed in lysing buffer (125 mM Tris-HCl [pH 6.8] containing 5% 2-mercaptoethanol, 2% sodium dodecyl sulfate [SDS], and 10% glycerol), and solubilized by incubation at 100°C for 10 min.

Amplification:

Article Title: Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan
Article Snippet: All PCRs included 25 or 30 cycles of amplification with the conditions described in Table . .. A strain identified as an STEC strain was incubated on Trypticase soy agar (Difco) at 37°C for 18 h. Approximately 0.3 g of organisms was harvested and suspended in 3 ml of saline solution.

In Vitro:

Article Title: Global Analysis of Helicobacter pylori Gene Expression in Human Gastric Mucosa
Article Snippet: .. H. pylori were initially grown on Trypticase Soy Agar (Difco, Detroit, MI) plates containing 5% sheep’s blood agar (BA) incubated at 37°C in a 5% CO2 incubator for 96 hours before being frozen as seed stocks for in vitro cultures. .. For analysis of in vitro RNA expression, 3 mL of sulfite-free brucella broth (containing 10% fetal bovine serum and 15 μg/mL of vancomycin) was inoculated to an optical density (OD) of 0.1 (OD at 600 nm) with H. pylori collected in this media after 24 hours of growth on BA plates.

Modification:

Article Title: Comparison of the Effects of Environmental Parameters on Growth Rates of Vibrio vulnificus Biotypes I, II, and III by Culture and Quantitative PCR Analysis ▿
Article Snippet: The pellet was then resuspended in 50 ml of defined (modified M9) minimal medium (23.2 mM Na2 HPO4 , 11.02 mM KH2 PO4 , 9.34 mM NH4 Cl, 1.0 mM MgSO4 ) augmented with 0.06% Casamino Acids, 0.006% yeast extract (Difco Laboratories Inc., Detroit, MI), and 4.5 mM glucose. .. Dilution series were made in 0.85% NaCl, and plating was performed on Trypticase soy agar (Difco Laboratories Inc., Detroit, MI) supplemented with NaCl (5.0 g liter−1 ).

Isolation:

Article Title: Actinobacillus actinomycetemcomitans Serotype d-Specific Antigen Contains the O Antigen of Lipopolysaccharide
Article Snippet: Paragraph title: Isolation of LPS. ... Briefly, the strains were grown on Trypticase soy agar (Difco Laboratories, Detroit, Mich.) plates in 5% CO2 in air at 37°C for 2 to 3 days.

Article Title: Global Analysis of Helicobacter pylori Gene Expression in Human Gastric Mucosa
Article Snippet: H. pylori strains B128 and B213 were isolated from human gastric biopsy specimens as previously described. .. H. pylori were initially grown on Trypticase Soy Agar (Difco, Detroit, MI) plates containing 5% sheep’s blood agar (BA) incubated at 37°C in a 5% CO2 incubator for 96 hours before being frozen as seed stocks for in vitro cultures.

Negative Control:

Article Title: Efficacy assessment of commercially available natural products and antibiotics, commonly used for mitigation of pathogenic Vibrio outbreaks in Ecuadorian Penaeus (Litopenaeus) vannamei hatcheries
Article Snippet: Eighty tubes were used to test the pathogenicity of the 20 bacterial isolates (four replicates per strain) and four tubes were used for the negative control (without bacteria, four replicates). .. Bacteria were activated on trypticase soy agar (TSA, Difco) and a colony from each strain was transferred to 150 mL of TSB and incubated for seven hours at 30°C.

Infection:

Article Title: Efficacy assessment of commercially available natural products and antibiotics, commonly used for mitigation of pathogenic Vibrio outbreaks in Ecuadorian Penaeus (Litopenaeus) vannamei hatcheries
Article Snippet: Bacteria were activated on trypticase soy agar (TSA, Difco) and a colony from each strain was transferred to 150 mL of TSB and incubated for seven hours at 30°C. .. Nauplii from all treatments and control were fed once with an inactivated V . alginolyticus commercial probiotic (107 ) [ ] four hours after infection.

Purification:

Article Title: Identification and Characterization of a Novel Terrabacter ginsenosidimutans sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ †
Article Snippet: Growth on nutrient agar and Trypticase soy agar (Difco, Detroit, MI) was also evaluated at 30°C. .. The crude n -hexane-quinone solution was purified using silica Sep-Pak Vac cartridges (Waters) and subsequently analyzed by HPLC, as previously described by Collins ( ).

Concentration Assay:

Article Title: A molecular survey of S. mutans and P. gingivalis oral microbial burden in human saliva using Relative Endpoint Polymerase Chain Reaction (RE-PCR) within the population of a Nevada dental school revealed disparities among minorities
Article Snippet: DNA was extracted from standard volumes of each sample (100 μL), as described above, and DNA purity and concentration were assessed. .. In brief, bacteria were plated and grown overnight at 37°C on Trypticase soy agar; SM plates were supplemented with 5% defibrinated sheep’s blood and PG were supplemented with 1% yeast extract from Difco (Sparks, MD).

Incubation:

Article Title: Culture Enriched Molecular Profiling of the Cystic Fibrosis Airway Microbiome
Article Snippet: Plates were incubated at 35°C in the presence of 5% CO2 for two days with the exception of OFPBL cultures, which were incubated at 30°C and chocolate agar cultures which were incubated anaerobically. .. Additional culture media included brain heart infusion (BHI) agar (Bacto) (with and without the addition of colistin sulfate and oxolinic acid to 10 mg/L and 5 mg/L, respectively), Columbia-colisitin-nalidixic acid (CNA) agar (BBL) with 5% sheep blood, fastidious anaerobe agar (FAA; Acumedia) with 5% sheep blood, kanamycin (75 mg/L)–vancomycin (7.5 mg/L) laked blood (5%) agar (KVLB; using FAA as the base), MAC (Difco), McKay agar , MSA (BBL), phenylethyl alcohol agar (PEA; BBL) with 5% sheep blood and trypticase soy agar (Difco) supplemented with 0.3% yeast extract (TSY; Bacto).

Article Title: Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan
Article Snippet: .. A strain identified as an STEC strain was incubated on Trypticase soy agar (Difco) at 37°C for 18 h. Approximately 0.3 g of organisms was harvested and suspended in 3 ml of saline solution. ..

Article Title: Identification and Characterization of a Novel Terrabacter ginsenosidimutans sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ †
Article Snippet: Salt tolerance was tested on R2A medium supplemented with 1 to 15% (wt/vol) NaCl after incubation for 5 days. .. Growth on nutrient agar and Trypticase soy agar (Difco, Detroit, MI) was also evaluated at 30°C.

Article Title: Human endothelial cells are activated by interferon-? plus tumour necrosis factor-? to kill intracellular Pseudomonas aeruginosa
Article Snippet: After each incubation period, cells from at least three wells were rinsed and lysed with 0·1% Triton-X-100 in PBS. .. Cell lysates were diluted and plated in trypticase soy agar (TSA; Difco) to quantify viable IC bacteria.

Article Title: Actinobacillus actinomycetemcomitans Serotype d-Specific Antigen Contains the O Antigen of Lipopolysaccharide
Article Snippet: Briefly, the strains were grown on Trypticase soy agar (Difco Laboratories, Detroit, Mich.) plates in 5% CO2 in air at 37°C for 2 to 3 days. .. The total bacterial cell membrane fraction was recovered by centrifugation at 100,000 × g for 1 h and treated with Sarkosyl (sodium lauryl sarcosinate) (Sigma Chemical Co., St. Louis, Mo.) at room temperature for 1 h, and the solution was centrifuged at 100,000 × g for 1 h. The pellet was suspended in distilled water, dispersed in lysing buffer (125 mM Tris-HCl [pH 6.8] containing 5% 2-mercaptoethanol, 2% sodium dodecyl sulfate [SDS], and 10% glycerol), and solubilized by incubation at 100°C for 10 min.

Article Title: Efficacy assessment of commercially available natural products and antibiotics, commonly used for mitigation of pathogenic Vibrio outbreaks in Ecuadorian Penaeus (Litopenaeus) vannamei hatcheries
Article Snippet: .. Bacteria were activated on trypticase soy agar (TSA, Difco) and a colony from each strain was transferred to 150 mL of TSB and incubated for seven hours at 30°C. ..

Article Title: Improved method for rapid and accurate isolation and identification of Streptococcus mutans and Streptococcus sobrinus from human plaque samples
Article Snippet: .. During the 48 h incubation of the MS ID plates (at 24 h or when sufficient colony growth was observed), plates were removed briefly from the incubator and a single colony from each MS ID plate was streaked onto Tryptic Soy Broth agar with 0.5% yeast extract (TSB-YE) (Difco, Sparks, MD, USA) to be used for rapid DNA extraction and freezer stocks of each isolate. .. TSB-YE plates were streaked from the SMAB side of the MS ID plates due to more rapid growth and more butyrous colonies.

Article Title: Global Analysis of Helicobacter pylori Gene Expression in Human Gastric Mucosa
Article Snippet: .. H. pylori were initially grown on Trypticase Soy Agar (Difco, Detroit, MI) plates containing 5% sheep’s blood agar (BA) incubated at 37°C in a 5% CO2 incubator for 96 hours before being frozen as seed stocks for in vitro cultures. .. For analysis of in vitro RNA expression, 3 mL of sulfite-free brucella broth (containing 10% fetal bovine serum and 15 μg/mL of vancomycin) was inoculated to an optical density (OD) of 0.1 (OD at 600 nm) with H. pylori collected in this media after 24 hours of growth on BA plates.

Article Title: A molecular survey of S. mutans and P. gingivalis oral microbial burden in human saliva using Relative Endpoint Polymerase Chain Reaction (RE-PCR) within the population of a Nevada dental school revealed disparities among minorities
Article Snippet: In brief, bacteria were plated and grown overnight at 37°C on Trypticase soy agar; SM plates were supplemented with 5% defibrinated sheep’s blood and PG were supplemented with 1% yeast extract from Difco (Sparks, MD). .. Single plate colonies were then inoculated into liquid broth; Trypticase soy broth for SM and supplemented tryptic soy broth for PG from Difco and incubated overnight at 37°C.

RNA Expression:

Article Title: Global Analysis of Helicobacter pylori Gene Expression in Human Gastric Mucosa
Article Snippet: H. pylori were initially grown on Trypticase Soy Agar (Difco, Detroit, MI) plates containing 5% sheep’s blood agar (BA) incubated at 37°C in a 5% CO2 incubator for 96 hours before being frozen as seed stocks for in vitro cultures. .. For analysis of in vitro RNA expression, 3 mL of sulfite-free brucella broth (containing 10% fetal bovine serum and 15 μg/mL of vancomycin) was inoculated to an optical density (OD) of 0.1 (OD at 600 nm) with H. pylori collected in this media after 24 hours of growth on BA plates.

Activity Assay:

Article Title: Identification and Characterization of a Novel Terrabacter ginsenosidimutans sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ †
Article Snippet: Physiological and biochemical characterization assays, including Gram staining, anaerobic growth, and catalase activity, were carried out as described by Smibert and Krieg ( ). .. Growth on nutrient agar and Trypticase soy agar (Difco, Detroit, MI) was also evaluated at 30°C.

Mass Spectrometry:

Article Title: Improved method for rapid and accurate isolation and identification of Streptococcus mutans and Streptococcus sobrinus from human plaque samples
Article Snippet: .. During the 48 h incubation of the MS ID plates (at 24 h or when sufficient colony growth was observed), plates were removed briefly from the incubator and a single colony from each MS ID plate was streaked onto Tryptic Soy Broth agar with 0.5% yeast extract (TSB-YE) (Difco, Sparks, MD, USA) to be used for rapid DNA extraction and freezer stocks of each isolate. .. TSB-YE plates were streaked from the SMAB side of the MS ID plates due to more rapid growth and more butyrous colonies.

Staining:

Article Title: Identification and Characterization of a Novel Terrabacter ginsenosidimutans sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ sp. nov. ?-Glucosidase That Transforms Ginsenoside Rb1 into the Rare Gypenosides XVII and LXXV ▿ †
Article Snippet: Physiological and biochemical characterization assays, including Gram staining, anaerobic growth, and catalase activity, were carried out as described by Smibert and Krieg ( ). .. Growth on nutrient agar and Trypticase soy agar (Difco, Detroit, MI) was also evaluated at 30°C.

Polymerase Chain Reaction:

Article Title: Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan
Article Snippet: The STEC isolates selected were characterized by PCR to determine the presence of bfp , the EAF plasmid, EHEC hlyA , and the LEE genes eae , espA , espB , espD , and tir (Table ). .. A strain identified as an STEC strain was incubated on Trypticase soy agar (Difco) at 37°C for 18 h. Approximately 0.3 g of organisms was harvested and suspended in 3 ml of saline solution.

Article Title: Improved method for rapid and accurate isolation and identification of Streptococcus mutans and Streptococcus sobrinus from human plaque samples
Article Snippet: Paragraph title: 2.4. Rapid DNA extraction and PCR ... During the 48 h incubation of the MS ID plates (at 24 h or when sufficient colony growth was observed), plates were removed briefly from the incubator and a single colony from each MS ID plate was streaked onto Tryptic Soy Broth agar with 0.5% yeast extract (TSB-YE) (Difco, Sparks, MD, USA) to be used for rapid DNA extraction and freezer stocks of each isolate.

Sonication:

Article Title: Actinobacillus actinomycetemcomitans Serotype d-Specific Antigen Contains the O Antigen of Lipopolysaccharide
Article Snippet: Briefly, the strains were grown on Trypticase soy agar (Difco Laboratories, Detroit, Mich.) plates in 5% CO2 in air at 37°C for 2 to 3 days. .. Bacterial colonies were suspended in phosphate-buffered saline (10 mM phosphate [pH 7.4], 150 mM NaCl) (PBS), and the cells were broken by sonication for 4.5 min on ice.

Crocin Bleaching Assay:

Article Title: Culture Enriched Molecular Profiling of the Cystic Fibrosis Airway Microbiome
Article Snippet: Solid media included Columbia blood agar (CBA) (Difco) with 5% sheep blood (Med-Ox), CHOC (GC base (Difco), hemoglobin (Gibco), IsoVitaleX enrichment (BBL)), chocolate agar (CHOC), MacConkey agar (MAC), mannitol-salt agar (MSA) and oxidation-fermentation polymyxin bacitracin lactose (OFPBL) agar. .. Additional culture media included brain heart infusion (BHI) agar (Bacto) (with and without the addition of colistin sulfate and oxolinic acid to 10 mg/L and 5 mg/L, respectively), Columbia-colisitin-nalidixic acid (CNA) agar (BBL) with 5% sheep blood, fastidious anaerobe agar (FAA; Acumedia) with 5% sheep blood, kanamycin (75 mg/L)–vancomycin (7.5 mg/L) laked blood (5%) agar (KVLB; using FAA as the base), MAC (Difco), McKay agar , MSA (BBL), phenylethyl alcohol agar (PEA; BBL) with 5% sheep blood and trypticase soy agar (Difco) supplemented with 0.3% yeast extract (TSY; Bacto).

other:

Article Title: Characterization of a Staphylococcal Plasmid Related to pUB110 and Carrying Two Novel Genes, vatC and vgbB, Encoding Resistance to Streptogramins A and B and Similar Antibiotics
Article Snippet: Trypticase soy broth or Trypticase soy agar (Difco Laboratories) was used for the detection of PIIA and PIB inactivation.

Article Title: Erosion and Subsequent Transport State of Escherichia coli from Cowpats
Article Snippet: Isolates were stored in cryovials (MicroBank; Pro-Lab Diagnostics, Richmond Hill, Ontario, Canada), resuscitated on Trypticase soy agar (Difco), and grown in Trypticase soy broth (Difco) for 16 to 18 h at 37°C before use.

Plasmid Preparation:

Article Title: Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan
Article Snippet: The STEC isolates selected were characterized by PCR to determine the presence of bfp , the EAF plasmid, EHEC hlyA , and the LEE genes eae , espA , espB , espD , and tir (Table ). .. A strain identified as an STEC strain was incubated on Trypticase soy agar (Difco) at 37°C for 18 h. Approximately 0.3 g of organisms was harvested and suspended in 3 ml of saline solution.

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  • 93
    Difco trypticase soy agar plates
    Experimental protocol. TSB , <t>trypticase</t> soy broth
    Trypticase Soy Agar Plates, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar plates/product/Difco
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar plates - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    86
    Difco trypticase soy blood agar
    Viscosity of the spent culture media obtained from Prevotella intermedia strains 17, OD1-16 and ATCC 25611 and Porphyromonas gingivalis strains ATCC 33277, 381 and W83 . Viscosity of the enriched <t>trypticase</t> soy broth was measured as a control. Bars indicate standard deviations. *P
    Trypticase Soy Blood Agar, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy blood agar/product/Difco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypticase soy blood agar - by Bioz Stars, 2020-04
    86/100 stars
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    84
    Difco robust biofilm formation
    Effect of heparin on known <t>biofilm</t> formation mutants. A. Mutants (gene names noted) were assessed for biofilm formation in the microtiter dish assay (8-h biofilms) with heparin at 1,000 U/ml (shaded bars) or with saline (white bars). B. Relative PIA levels
    Robust Biofilm Formation, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/robust biofilm formation/product/Difco
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    robust biofilm formation - by Bioz Stars, 2020-04
    84/100 stars
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    92
    Difco trypticase soy agar tsa
    Effect of heparin on known <t>biofilm</t> formation mutants. A. Mutants (gene names noted) were assessed for biofilm formation in the microtiter dish assay (8-h biofilms) with heparin at 1,000 U/ml (shaded bars) or with saline (white bars). B. Relative PIA levels
    Trypticase Soy Agar Tsa, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar tsa/product/Difco
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar tsa - by Bioz Stars, 2020-04
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      Buy from Supplier

    Image Search Results


    Experimental protocol. TSB , trypticase soy broth

    Journal: The Journal of urology

    Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS

    doi:

    Figure Lengend Snippet: Experimental protocol. TSB , trypticase soy broth

    Article Snippet: Just before performing the catheter studies bacteria were retrieved from the trypticase soy agar plates and cultured overnight in trypticase soy broth (Difco, Sparks, Maryland).

    Techniques:

    Viscosity of the spent culture media obtained from Prevotella intermedia strains 17, OD1-16 and ATCC 25611 and Porphyromonas gingivalis strains ATCC 33277, 381 and W83 . Viscosity of the enriched trypticase soy broth was measured as a control. Bars indicate standard deviations. *P

    Journal: BMC Infectious Diseases

    Article Title: Comparison of the virulence of exopolysaccharide-producing Prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms

    doi: 10.1186/1471-2334-11-228

    Figure Lengend Snippet: Viscosity of the spent culture media obtained from Prevotella intermedia strains 17, OD1-16 and ATCC 25611 and Porphyromonas gingivalis strains ATCC 33277, 381 and W83 . Viscosity of the enriched trypticase soy broth was measured as a control. Bars indicate standard deviations. *P

    Article Snippet: These bacterial strains were grown on trypticase soy blood agar plates supplemented with 0.5% yeast extract (Difco Laboratories, Detroit, MI), hemin (5 mg/l), L-cystine (400 mg/l), and vitamin K1 (10 mg/l) (enriched BAP) or grown in trypticase soy broth (BBL Microbiology Systems, Cockeysville, ND) supplemented with 0.5% yeast extract, hemin (5 mg/l), L-cystine (400 mg/l), and vitamin K1 (10 mg/l) (enriched TSB).

    Techniques:

    Effect of heparin on known biofilm formation mutants. A. Mutants (gene names noted) were assessed for biofilm formation in the microtiter dish assay (8-h biofilms) with heparin at 1,000 U/ml (shaded bars) or with saline (white bars). B. Relative PIA levels

    Journal:

    Article Title: Heparin Stimulates Staphylococcus aureus Biofilm Formation

    doi: 10.1128/IAI.73.8.4596-4606.2005

    Figure Lengend Snippet: Effect of heparin on known biofilm formation mutants. A. Mutants (gene names noted) were assessed for biofilm formation in the microtiter dish assay (8-h biofilms) with heparin at 1,000 U/ml (shaded bars) or with saline (white bars). B. Relative PIA levels

    Article Snippet: For all phenotypic assays we use 66% TSB plus 0.2% glucose, as this medium promotes robust biofilm formation (data not shown) (20 g/liter of Difco Bacto tryptic soy broth, Becton Dickinson, Sparks, MD).

    Techniques:

    Sodium heparin enhances S. aureus biofilm formation. The effect of heparin on the formation of S. aureus MZ100 biofilms on abiotic surfaces was assessed microscopically. Scanning electron micrographs of 12-hour-old S. aureus biofilms on polyvinylchloride

    Journal:

    Article Title: Heparin Stimulates Staphylococcus aureus Biofilm Formation

    doi: 10.1128/IAI.73.8.4596-4606.2005

    Figure Lengend Snippet: Sodium heparin enhances S. aureus biofilm formation. The effect of heparin on the formation of S. aureus MZ100 biofilms on abiotic surfaces was assessed microscopically. Scanning electron micrographs of 12-hour-old S. aureus biofilms on polyvinylchloride

    Article Snippet: For all phenotypic assays we use 66% TSB plus 0.2% glucose, as this medium promotes robust biofilm formation (data not shown) (20 g/liter of Difco Bacto tryptic soy broth, Becton Dickinson, Sparks, MD).

    Techniques:

    Sodium heparin increases the adherence of S. aureus to polystyrene. A. Dose response. Serial dilutions of sodium heparin were added to cultures and biofilms were allowed to form for 16 h, at which time nonadherent cells were removed by vigorous washing.

    Journal:

    Article Title: Heparin Stimulates Staphylococcus aureus Biofilm Formation

    doi: 10.1128/IAI.73.8.4596-4606.2005

    Figure Lengend Snippet: Sodium heparin increases the adherence of S. aureus to polystyrene. A. Dose response. Serial dilutions of sodium heparin were added to cultures and biofilms were allowed to form for 16 h, at which time nonadherent cells were removed by vigorous washing.

    Article Snippet: For all phenotypic assays we use 66% TSB plus 0.2% glucose, as this medium promotes robust biofilm formation (data not shown) (20 g/liter of Difco Bacto tryptic soy broth, Becton Dickinson, Sparks, MD).

    Techniques:

    Sodium heparin enhances S. aureus biofilm formation. The effect of heparin formation of S. aureus (MZ100) biofilms on abiotic surfaces was assessed microscopically. S. aureus biofilms (4 hours) formed on polystyrene were viewed with phase 2 microscopy

    Journal:

    Article Title: Heparin Stimulates Staphylococcus aureus Biofilm Formation

    doi: 10.1128/IAI.73.8.4596-4606.2005

    Figure Lengend Snippet: Sodium heparin enhances S. aureus biofilm formation. The effect of heparin formation of S. aureus (MZ100) biofilms on abiotic surfaces was assessed microscopically. S. aureus biofilms (4 hours) formed on polystyrene were viewed with phase 2 microscopy

    Article Snippet: For all phenotypic assays we use 66% TSB plus 0.2% glucose, as this medium promotes robust biofilm formation (data not shown) (20 g/liter of Difco Bacto tryptic soy broth, Becton Dickinson, Sparks, MD).

    Techniques: Microscopy