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Becton Dickinson trypticase soy agar
Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and <t>Trypticase</t> soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was
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1) Product Images from "Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine"

Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine

Journal: Infection and Immunity

doi: 10.1128/IAI.69.11.6823-6830.2001

Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and Trypticase soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was
Figure Legend Snippet: Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and Trypticase soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was

Techniques Used: Cell Culture, Staining

2) Product Images from "Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression"

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression

Journal: Molecular & Cellular Proteomics : MCP

doi: 10.1074/mcp.RA118.001181

H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p
Figure Legend Snippet: H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p

Techniques Used: Infection, Isolation, Incubation, Staining, MANN-WHITNEY

3) Product Images from "Cutaneous Infection Caused by Gordonia amicalis after a Traumatic Injury"

Article Title: Cutaneous Infection Caused by Gordonia amicalis after a Traumatic Injury

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.06681-11

Orange, opaque, dry, and nonhemolytic colonies grew on Trypticase soy agar supplemented with 5% sheep blood after incubation for 72 h.
Figure Legend Snippet: Orange, opaque, dry, and nonhemolytic colonies grew on Trypticase soy agar supplemented with 5% sheep blood after incubation for 72 h.

Techniques Used: Incubation

4) Product Images from "Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System"

Article Title: Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System

Journal: Oncogene

doi: 10.1038/onc.2016.158

H. pylori activation of TLR9 requires a functional cag T4SS (a–c) The H. pylori cag + strains J166 and 26695 (ATCC 700392) were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences, Sparks MD USA) as described ( 48 , 58 ). Isogenic mutants were constructed as previously described ( 48 , 55 , 58 – 62 ). Flanking sequences for comB were amplified from H. pylori strain J166 DNA using primers comB8 Forward (5′-ACTAGAGCTCAAGCCTTTCAATAGCGAGCA- 3′), comB8 Reverse (5′-AGTACCGCGGAGCGATTTTCAAGCGGTTC -3’) and comB10 Forward (5’-CTGAGAATTCTTGCAATTGATGAGGCAAAG-3′) comB10 Reverse (5′-ACTAGGTACCGCGATGACTTCATTCTCTCTGG -3′). comB flanking sequences were cloned into a pBSC103 plasmid using a previously inserted kanamycin resistance cassette generated by restriction enzymes SacI and SacII ( comB8 ) and EcoRI and KpnI ( comB10 ). The resultant plasmid was used to transform H. pylori strain J166 and transformants were selected on Brucella agar plates supplemented with kanamycin (5 μg/mL). Correct orientation of the kanamyacin cassette with H. pylori comB was confirmed by PCR analyses. (a) H. pylori strain J166 DNA was purified by growing microbial cultures in Brucella broth supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals, Atlanta, GA USA) overnight. Cultures were centrifuged (4000 RPM, 5 min), resuspended in 600 μL of TE buffer with 0.5% SDS and 100 μg/mL proteinase K (Qiagen Germantown MD USA), and incubated at 37°C for 1 hour. DNA was extracted using CTAB and purified by phenol-chloroform extraction. TLR9-reporter or parental cells were cultured as described in Figure 1 and challenged with purified H. pylori strain J166 DNA (1–5 μg/mL) supplemented with Lipofectamine 2000 (Life Technologies, Carlsbad CA, USA) at 37°C with 5% CO 2 for 24 hours. Data are represented as fold over vehicle control. (b) TLR9 activation induced by H. pylori strain J166 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (c) TLR9 activation induced by H. pylori strain 26695 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (d) AGS gastric epithelial cells (ATCC) were grown in RPMI (Cell Gro, Manassas, VA, USA) supplemented with 5% fetal bovine serum (Atlanta Biologicals). Cells were seeded at 500 000 cells per well in a 12-well culture dish (Corning) and infected with either H. pylori strains J166 or 26695 (MOI 100), their respective cagE − mutants (MOI 100), or their respective purified gDNA (5 μg/mL) for 6 hours. Levels of IL-8 were quantified using Human CXCL8 ELISA (R D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions and tested in duplicate. (e) Liquid cultures of H. pylori were grown with shaking overnight in 5 mL of Brucella broth (BD Biosciences) supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals) at 37°C and 5% CO 2 . Supernatants of overnight cultures were collected, filtered (0.45 μm filter) and used for TLR9 activation assays at concentrations ranging from 1–30%. Results are shown relative to vehicle (Brucella broth) control. (f) HEK293 reporter cells were co-cultured with H. pylori wild type or isogenic mutant strains of J166 at an MOI of 100 for 4 hours. Cells were washed 3 times with PBS containing gentamicin (250 μg/mL; Corning). Cells were then incubated at 37°C for an additional hour in RPMI containing gentamicin (250 μg/mL), washed 3 times with PBS, lysed in 200 μL of sterile dH2O and serial dilutions were plated on TSA plates with 5% sheep blood (BD Biosciences). Plates were incubated for 5 days at 37°C, 5% CO 2 and colonies were enumerated. Experiments were repeated at least 3 times. Viable colony-forming units with mean±SEM are shown. Student’s t- test (a) or one-way analysis of variance with Bonferroni correction (b–f) was used to determine statistical significance between groups. *p
Figure Legend Snippet: H. pylori activation of TLR9 requires a functional cag T4SS (a–c) The H. pylori cag + strains J166 and 26695 (ATCC 700392) were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences, Sparks MD USA) as described ( 48 , 58 ). Isogenic mutants were constructed as previously described ( 48 , 55 , 58 – 62 ). Flanking sequences for comB were amplified from H. pylori strain J166 DNA using primers comB8 Forward (5′-ACTAGAGCTCAAGCCTTTCAATAGCGAGCA- 3′), comB8 Reverse (5′-AGTACCGCGGAGCGATTTTCAAGCGGTTC -3’) and comB10 Forward (5’-CTGAGAATTCTTGCAATTGATGAGGCAAAG-3′) comB10 Reverse (5′-ACTAGGTACCGCGATGACTTCATTCTCTCTGG -3′). comB flanking sequences were cloned into a pBSC103 plasmid using a previously inserted kanamycin resistance cassette generated by restriction enzymes SacI and SacII ( comB8 ) and EcoRI and KpnI ( comB10 ). The resultant plasmid was used to transform H. pylori strain J166 and transformants were selected on Brucella agar plates supplemented with kanamycin (5 μg/mL). Correct orientation of the kanamyacin cassette with H. pylori comB was confirmed by PCR analyses. (a) H. pylori strain J166 DNA was purified by growing microbial cultures in Brucella broth supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals, Atlanta, GA USA) overnight. Cultures were centrifuged (4000 RPM, 5 min), resuspended in 600 μL of TE buffer with 0.5% SDS and 100 μg/mL proteinase K (Qiagen Germantown MD USA), and incubated at 37°C for 1 hour. DNA was extracted using CTAB and purified by phenol-chloroform extraction. TLR9-reporter or parental cells were cultured as described in Figure 1 and challenged with purified H. pylori strain J166 DNA (1–5 μg/mL) supplemented with Lipofectamine 2000 (Life Technologies, Carlsbad CA, USA) at 37°C with 5% CO 2 for 24 hours. Data are represented as fold over vehicle control. (b) TLR9 activation induced by H. pylori strain J166 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (c) TLR9 activation induced by H. pylori strain 26695 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (d) AGS gastric epithelial cells (ATCC) were grown in RPMI (Cell Gro, Manassas, VA, USA) supplemented with 5% fetal bovine serum (Atlanta Biologicals). Cells were seeded at 500 000 cells per well in a 12-well culture dish (Corning) and infected with either H. pylori strains J166 or 26695 (MOI 100), their respective cagE − mutants (MOI 100), or their respective purified gDNA (5 μg/mL) for 6 hours. Levels of IL-8 were quantified using Human CXCL8 ELISA (R D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions and tested in duplicate. (e) Liquid cultures of H. pylori were grown with shaking overnight in 5 mL of Brucella broth (BD Biosciences) supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals) at 37°C and 5% CO 2 . Supernatants of overnight cultures were collected, filtered (0.45 μm filter) and used for TLR9 activation assays at concentrations ranging from 1–30%. Results are shown relative to vehicle (Brucella broth) control. (f) HEK293 reporter cells were co-cultured with H. pylori wild type or isogenic mutant strains of J166 at an MOI of 100 for 4 hours. Cells were washed 3 times with PBS containing gentamicin (250 μg/mL; Corning). Cells were then incubated at 37°C for an additional hour in RPMI containing gentamicin (250 μg/mL), washed 3 times with PBS, lysed in 200 μL of sterile dH2O and serial dilutions were plated on TSA plates with 5% sheep blood (BD Biosciences). Plates were incubated for 5 days at 37°C, 5% CO 2 and colonies were enumerated. Experiments were repeated at least 3 times. Viable colony-forming units with mean±SEM are shown. Student’s t- test (a) or one-way analysis of variance with Bonferroni correction (b–f) was used to determine statistical significance between groups. *p

Techniques Used: Activation Assay, Functional Assay, Construct, Amplification, Clone Assay, Plasmid Preparation, Generated, Polymerase Chain Reaction, Purification, Incubation, Cell Culture, Infection, Enzyme-linked Immunosorbent Assay, Mutagenesis

5) Product Images from "Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model"

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

doi: 10.1093/jbcr/iry043

Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .
Figure Legend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

Techniques Used: Infection, Serial Dilution, Isolation

6) Product Images from "Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression"

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression

Journal: Molecular & Cellular Proteomics : MCP

doi: 10.1074/mcp.RA118.001181

H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p
Figure Legend Snippet: H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p

Techniques Used: Infection, Isolation, Incubation, Staining, MANN-WHITNEY

7) Product Images from "Chromobacterium violaceum infections in 13 non-human primates"

Article Title: Chromobacterium violaceum infections in 13 non-human primates

Journal: Journal of medical primatology

doi: 10.1111/j.1600-0684.2011.00529.x

Chromobacterium violaceum isolation (From the liver of Case No. 3). Distinctive violet pigmented colonies were developed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood media after anaerobic incubation for 24 hours at 37°C.
Figure Legend Snippet: Chromobacterium violaceum isolation (From the liver of Case No. 3). Distinctive violet pigmented colonies were developed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood media after anaerobic incubation for 24 hours at 37°C.

Techniques Used: Isolation, Modification, Incubation

8) Product Images from "Rinsability of Orthophthalaldehyde from Endoscopes"

Article Title: Rinsability of Orthophthalaldehyde from Endoscopes

Journal: Diagnostic and Therapeutic Endoscopy

doi: 10.1155/2012/853781

Typical zone of inhibition or no zone of inhibition on agar surfaces with a lawn of Staphylococcus aureus surrounding 2 cm × 1 cm sections of Viton endoscope bending rubber exposed from left to right to GA-IPA, GA, or OPA for 10.0 minutes, and then rinsed three times each with 100 mL of water. These positive control sections of the endoscope materials were then placed onto the trypticase soy agar surfaces with bacterial lawns. Negative control sections of the endoscope materials were soaked in water, without exposure to any disinfectant, and then placed onto the agar with bacterial lawns, and the result determined that there were no chemicals in the insertion tube materials able to give a ZOI in these tests (not shown).
Figure Legend Snippet: Typical zone of inhibition or no zone of inhibition on agar surfaces with a lawn of Staphylococcus aureus surrounding 2 cm × 1 cm sections of Viton endoscope bending rubber exposed from left to right to GA-IPA, GA, or OPA for 10.0 minutes, and then rinsed three times each with 100 mL of water. These positive control sections of the endoscope materials were then placed onto the trypticase soy agar surfaces with bacterial lawns. Negative control sections of the endoscope materials were soaked in water, without exposure to any disinfectant, and then placed onto the agar with bacterial lawns, and the result determined that there were no chemicals in the insertion tube materials able to give a ZOI in these tests (not shown).

Techniques Used: Inhibition, Indirect Immunoperoxidase Assay, Positive Control, Negative Control

9) Product Images from "Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression"

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression

Journal: Molecular & Cellular Proteomics : MCP

doi: 10.1074/mcp.RA118.001181

H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p
Figure Legend Snippet: H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p

Techniques Used: Infection, Isolation, Incubation, Staining, MANN-WHITNEY

10) Product Images from "Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model"

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

doi: 10.1093/jbcr/iry043

Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .
Figure Legend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

Techniques Used: Infection, Serial Dilution, Isolation

11) Product Images from "Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus"

Article Title: Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus

Journal: Immunology

doi: 10.1111/j.1365-2567.2008.02952.x

Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into trypticase soy broth in 10-fold serial dilutions of stock starting at 8 ×
Figure Legend Snippet: Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into trypticase soy broth in 10-fold serial dilutions of stock starting at 8 ×

Techniques Used:

12) Product Images from "Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns"

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.46.6.1837-1844.2002

Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.
Figure Legend Snippet: Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

Techniques Used: Incubation

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    Becton Dickinson trypticase blood agar plates
    Loss of CD73 decreases survival, and increases bacterial load and inflammation in polymicrobial sepsis (A) The number of surviving CD73 WT and KO mice were counted daily for 7 days after inducing sepsis by cecal ligation and puncture (CLP). (B) Blood and peritoneal lavage fluid obtained from CD73 WT and KO mice 16 h post CLP were cultured on <t>soy-trypticase</t> agar plates for 24 h and then the bacterial colonies were counted (n = 13 mice per group). Serum and peritoneal lavage fluid concentration of TNF-α (C), IL-1β (D), IL12p40 (E), IL-6 (F), IL-10 (G), MCP-1 (H), MIP-1α (I) and MIP-2 (J) were determined in samples collected 16 h post CLP using ELISA. All results (mean ± SEM) shown are representative of three separate experiments. * p
    Trypticase Blood Agar Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase blood agar plates/product/Becton Dickinson
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    Becton Dickinson trypticase soy agar
    Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and <t>Trypticase</t> soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was
    Trypticase Soy Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2022-05
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    Becton Dickinson tsbye medium
    A. <t>actinomycetemcomitans</t> colony morphology. Bacteria were grown on solid <t>TSBYE</t> medium for 3 days at 37 °C in a humidified 10 % CO 2 atmosphere. Images were taken using a Leica MZ16F stereomicroscope. (a) WT (VT1169); (b)
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    Loss of CD73 decreases survival, and increases bacterial load and inflammation in polymicrobial sepsis (A) The number of surviving CD73 WT and KO mice were counted daily for 7 days after inducing sepsis by cecal ligation and puncture (CLP). (B) Blood and peritoneal lavage fluid obtained from CD73 WT and KO mice 16 h post CLP were cultured on soy-trypticase agar plates for 24 h and then the bacterial colonies were counted (n = 13 mice per group). Serum and peritoneal lavage fluid concentration of TNF-α (C), IL-1β (D), IL12p40 (E), IL-6 (F), IL-10 (G), MCP-1 (H), MIP-1α (I) and MIP-2 (J) were determined in samples collected 16 h post CLP using ELISA. All results (mean ± SEM) shown are representative of three separate experiments. * p

    Journal: Journal of Immunology (Baltimore, Md. : 1950)

    Article Title: Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis

    doi: 10.4049/jimmunol.1003379

    Figure Lengend Snippet: Loss of CD73 decreases survival, and increases bacterial load and inflammation in polymicrobial sepsis (A) The number of surviving CD73 WT and KO mice were counted daily for 7 days after inducing sepsis by cecal ligation and puncture (CLP). (B) Blood and peritoneal lavage fluid obtained from CD73 WT and KO mice 16 h post CLP were cultured on soy-trypticase agar plates for 24 h and then the bacterial colonies were counted (n = 13 mice per group). Serum and peritoneal lavage fluid concentration of TNF-α (C), IL-1β (D), IL12p40 (E), IL-6 (F), IL-10 (G), MCP-1 (H), MIP-1α (I) and MIP-2 (J) were determined in samples collected 16 h post CLP using ELISA. All results (mean ± SEM) shown are representative of three separate experiments. * p

    Article Snippet: Fifty μl of each dilution was aseptically plated and cultured on trypticase blood agar plates (BD Biosciences) at 37°C.

    Techniques: Mouse Assay, Ligation, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and Trypticase soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was

    Journal: Infection and Immunity

    Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine

    doi: 10.1128/IAI.69.11.6823-6830.2001

    Figure Lengend Snippet: Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and Trypticase soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was

    Article Snippet: Culture purity was verified by gram staining and plating on BGA and Trypticase soy agar (Becton-Dickinson).

    Techniques: Cell Culture, Staining

    Orange, opaque, dry, and nonhemolytic colonies grew on Trypticase soy agar supplemented with 5% sheep blood after incubation for 72 h.

    Journal: Journal of Clinical Microbiology

    Article Title: Cutaneous Infection Caused by Gordonia amicalis after a Traumatic Injury

    doi: 10.1128/JCM.06681-11

    Figure Lengend Snippet: Orange, opaque, dry, and nonhemolytic colonies grew on Trypticase soy agar supplemented with 5% sheep blood after incubation for 72 h.

    Article Snippet: Cultures on Trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Sparks, MD) grew small numbers of slightly orange and dry colonies and Gram-positive coryneform bacilli with rudimentary branching and weakly acid-fast bacilli after incubation for 4 days.

    Techniques: Incubation

    A. actinomycetemcomitans colony morphology. Bacteria were grown on solid TSBYE medium for 3 days at 37 °C in a humidified 10 % CO 2 atmosphere. Images were taken using a Leica MZ16F stereomicroscope. (a) WT (VT1169); (b)

    Journal: Microbiology

    Article Title: Inner-membrane protein MorC is involved in fimbriae production and biofilm formation in Aggregatibacter actinomycetemcomitans

    doi: 10.1099/mic.0.000246

    Figure Lengend Snippet: A. actinomycetemcomitans colony morphology. Bacteria were grown on solid TSBYE medium for 3 days at 37 °C in a humidified 10 % CO 2 atmosphere. Images were taken using a Leica MZ16F stereomicroscope. (a) WT (VT1169); (b)

    Article Snippet: A. actinomycetemcomitans strains were routinely cultured in TSBYE medium (3 % tryptic soy broth, 0.6 % yeast extract, with or without 1.5 % agar; Beckton Dickinson).

    Techniques: