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Becton Dickinson trypticase soy agar
Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or <t>Trypticase</t> soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .
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1) Product Images from "Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model"

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

doi: 10.1093/jbcr/iry043

Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .
Figure Legend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

Techniques Used: Infection, Serial Dilution, Isolation

2) Product Images from "Chromobacterium violaceum infections in 13 non-human primates"

Article Title: Chromobacterium violaceum infections in 13 non-human primates

Journal: Journal of medical primatology

doi: 10.1111/j.1600-0684.2011.00529.x

Chromobacterium violaceum isolation (From the liver of Case No. 3). Distinctive violet pigmented colonies were developed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood media after anaerobic incubation for 24 hours at 37°C.
Figure Legend Snippet: Chromobacterium violaceum isolation (From the liver of Case No. 3). Distinctive violet pigmented colonies were developed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood media after anaerobic incubation for 24 hours at 37°C.

Techniques Used: Isolation, Modification, Incubation

3) Product Images from "Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model"

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

doi: 10.1093/jbcr/iry043

Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .
Figure Legend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

Techniques Used: Infection, Serial Dilution, Isolation

4) Product Images from "Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression"

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression

Journal: Molecular & Cellular Proteomics : MCP

doi: 10.1074/mcp.RA118.001181

H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p
Figure Legend Snippet: H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p

Techniques Used: Infection, Isolation, Incubation, Staining, MANN-WHITNEY

5) Product Images from "Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System"

Article Title: Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System

Journal: Oncogene

doi: 10.1038/onc.2016.158

H. pylori activation of TLR9 requires a functional cag T4SS (a–c) The H. pylori cag + strains J166 and 26695 (ATCC 700392) were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences, Sparks MD USA) as described ( 48 , 58 ). Isogenic mutants were constructed as previously described ( 48 , 55 , 58 – 62 ). Flanking sequences for comB were amplified from H. pylori strain J166 DNA using primers comB8 Forward (5′-ACTAGAGCTCAAGCCTTTCAATAGCGAGCA- 3′), comB8 Reverse (5′-AGTACCGCGGAGCGATTTTCAAGCGGTTC -3’) and comB10 Forward (5’-CTGAGAATTCTTGCAATTGATGAGGCAAAG-3′) comB10 Reverse (5′-ACTAGGTACCGCGATGACTTCATTCTCTCTGG -3′). comB flanking sequences were cloned into a pBSC103 plasmid using a previously inserted kanamycin resistance cassette generated by restriction enzymes SacI and SacII ( comB8 ) and EcoRI and KpnI ( comB10 ). The resultant plasmid was used to transform H. pylori strain J166 and transformants were selected on Brucella agar plates supplemented with kanamycin (5 μg/mL). Correct orientation of the kanamyacin cassette with H. pylori comB was confirmed by PCR analyses. (a) H. pylori strain J166 DNA was purified by growing microbial cultures in Brucella broth supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals, Atlanta, GA USA) overnight. Cultures were centrifuged (4000 RPM, 5 min), resuspended in 600 μL of TE buffer with 0.5% SDS and 100 μg/mL proteinase K (Qiagen Germantown MD USA), and incubated at 37°C for 1 hour. DNA was extracted using CTAB and purified by phenol-chloroform extraction. TLR9-reporter or parental cells were cultured as described in Figure 1 and challenged with purified H. pylori strain J166 DNA (1–5 μg/mL) supplemented with Lipofectamine 2000 (Life Technologies, Carlsbad CA, USA) at 37°C with 5% CO 2 for 24 hours. Data are represented as fold over vehicle control. (b) TLR9 activation induced by H. pylori strain J166 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (c) TLR9 activation induced by H. pylori strain 26695 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (d) AGS gastric epithelial cells (ATCC) were grown in RPMI (Cell Gro, Manassas, VA, USA) supplemented with 5% fetal bovine serum (Atlanta Biologicals). Cells were seeded at 500 000 cells per well in a 12-well culture dish (Corning) and infected with either H. pylori strains J166 or 26695 (MOI 100), their respective cagE − mutants (MOI 100), or their respective purified gDNA (5 μg/mL) for 6 hours. Levels of IL-8 were quantified using Human CXCL8 ELISA (R D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions and tested in duplicate. (e) Liquid cultures of H. pylori were grown with shaking overnight in 5 mL of Brucella broth (BD Biosciences) supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals) at 37°C and 5% CO 2 . Supernatants of overnight cultures were collected, filtered (0.45 μm filter) and used for TLR9 activation assays at concentrations ranging from 1–30%. Results are shown relative to vehicle (Brucella broth) control. (f) HEK293 reporter cells were co-cultured with H. pylori wild type or isogenic mutant strains of J166 at an MOI of 100 for 4 hours. Cells were washed 3 times with PBS containing gentamicin (250 μg/mL; Corning). Cells were then incubated at 37°C for an additional hour in RPMI containing gentamicin (250 μg/mL), washed 3 times with PBS, lysed in 200 μL of sterile dH2O and serial dilutions were plated on TSA plates with 5% sheep blood (BD Biosciences). Plates were incubated for 5 days at 37°C, 5% CO 2 and colonies were enumerated. Experiments were repeated at least 3 times. Viable colony-forming units with mean±SEM are shown. Student’s t- test (a) or one-way analysis of variance with Bonferroni correction (b–f) was used to determine statistical significance between groups. *p
Figure Legend Snippet: H. pylori activation of TLR9 requires a functional cag T4SS (a–c) The H. pylori cag + strains J166 and 26695 (ATCC 700392) were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences, Sparks MD USA) as described ( 48 , 58 ). Isogenic mutants were constructed as previously described ( 48 , 55 , 58 – 62 ). Flanking sequences for comB were amplified from H. pylori strain J166 DNA using primers comB8 Forward (5′-ACTAGAGCTCAAGCCTTTCAATAGCGAGCA- 3′), comB8 Reverse (5′-AGTACCGCGGAGCGATTTTCAAGCGGTTC -3’) and comB10 Forward (5’-CTGAGAATTCTTGCAATTGATGAGGCAAAG-3′) comB10 Reverse (5′-ACTAGGTACCGCGATGACTTCATTCTCTCTGG -3′). comB flanking sequences were cloned into a pBSC103 plasmid using a previously inserted kanamycin resistance cassette generated by restriction enzymes SacI and SacII ( comB8 ) and EcoRI and KpnI ( comB10 ). The resultant plasmid was used to transform H. pylori strain J166 and transformants were selected on Brucella agar plates supplemented with kanamycin (5 μg/mL). Correct orientation of the kanamyacin cassette with H. pylori comB was confirmed by PCR analyses. (a) H. pylori strain J166 DNA was purified by growing microbial cultures in Brucella broth supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals, Atlanta, GA USA) overnight. Cultures were centrifuged (4000 RPM, 5 min), resuspended in 600 μL of TE buffer with 0.5% SDS and 100 μg/mL proteinase K (Qiagen Germantown MD USA), and incubated at 37°C for 1 hour. DNA was extracted using CTAB and purified by phenol-chloroform extraction. TLR9-reporter or parental cells were cultured as described in Figure 1 and challenged with purified H. pylori strain J166 DNA (1–5 μg/mL) supplemented with Lipofectamine 2000 (Life Technologies, Carlsbad CA, USA) at 37°C with 5% CO 2 for 24 hours. Data are represented as fold over vehicle control. (b) TLR9 activation induced by H. pylori strain J166 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (c) TLR9 activation induced by H. pylori strain 26695 or its isogenic mutants (MOI 100, T=24hrs), relative to uninfected control. (d) AGS gastric epithelial cells (ATCC) were grown in RPMI (Cell Gro, Manassas, VA, USA) supplemented with 5% fetal bovine serum (Atlanta Biologicals). Cells were seeded at 500 000 cells per well in a 12-well culture dish (Corning) and infected with either H. pylori strains J166 or 26695 (MOI 100), their respective cagE − mutants (MOI 100), or their respective purified gDNA (5 μg/mL) for 6 hours. Levels of IL-8 were quantified using Human CXCL8 ELISA (R D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions and tested in duplicate. (e) Liquid cultures of H. pylori were grown with shaking overnight in 5 mL of Brucella broth (BD Biosciences) supplemented with 10% neonatal calf serum (NCS) (Atlanta Biologicals) at 37°C and 5% CO 2 . Supernatants of overnight cultures were collected, filtered (0.45 μm filter) and used for TLR9 activation assays at concentrations ranging from 1–30%. Results are shown relative to vehicle (Brucella broth) control. (f) HEK293 reporter cells were co-cultured with H. pylori wild type or isogenic mutant strains of J166 at an MOI of 100 for 4 hours. Cells were washed 3 times with PBS containing gentamicin (250 μg/mL; Corning). Cells were then incubated at 37°C for an additional hour in RPMI containing gentamicin (250 μg/mL), washed 3 times with PBS, lysed in 200 μL of sterile dH2O and serial dilutions were plated on TSA plates with 5% sheep blood (BD Biosciences). Plates were incubated for 5 days at 37°C, 5% CO 2 and colonies were enumerated. Experiments were repeated at least 3 times. Viable colony-forming units with mean±SEM are shown. Student’s t- test (a) or one-way analysis of variance with Bonferroni correction (b–f) was used to determine statistical significance between groups. *p

Techniques Used: Activation Assay, Functional Assay, Construct, Amplification, Clone Assay, Plasmid Preparation, Generated, Polymerase Chain Reaction, Purification, Incubation, Cell Culture, Infection, Enzyme-linked Immunosorbent Assay, Mutagenesis

6) Product Images from "Rinsability of Orthophthalaldehyde from Endoscopes"

Article Title: Rinsability of Orthophthalaldehyde from Endoscopes

Journal: Diagnostic and Therapeutic Endoscopy

doi: 10.1155/2012/853781

Typical zone of inhibition or no zone of inhibition on agar surfaces with a lawn of Staphylococcus aureus surrounding 2 cm × 1 cm sections of Viton endoscope bending rubber exposed from left to right to GA-IPA, GA, or OPA for 10.0 minutes, and then rinsed three times each with 100 mL of water. These positive control sections of the endoscope materials were then placed onto the trypticase soy agar surfaces with bacterial lawns. Negative control sections of the endoscope materials were soaked in water, without exposure to any disinfectant, and then placed onto the agar with bacterial lawns, and the result determined that there were no chemicals in the insertion tube materials able to give a ZOI in these tests (not shown).
Figure Legend Snippet: Typical zone of inhibition or no zone of inhibition on agar surfaces with a lawn of Staphylococcus aureus surrounding 2 cm × 1 cm sections of Viton endoscope bending rubber exposed from left to right to GA-IPA, GA, or OPA for 10.0 minutes, and then rinsed three times each with 100 mL of water. These positive control sections of the endoscope materials were then placed onto the trypticase soy agar surfaces with bacterial lawns. Negative control sections of the endoscope materials were soaked in water, without exposure to any disinfectant, and then placed onto the agar with bacterial lawns, and the result determined that there were no chemicals in the insertion tube materials able to give a ZOI in these tests (not shown).

Techniques Used: Inhibition, Indirect Immunoperoxidase Assay, Positive Control, Negative Control

7) Product Images from "Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression"

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression

Journal: Molecular & Cellular Proteomics : MCP

doi: 10.1074/mcp.RA118.001181

H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p
Figure Legend Snippet: H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p

Techniques Used: Infection, Isolation, Incubation, Staining, MANN-WHITNEY

8) Product Images from "Cutaneous Infection Caused by Gordonia amicalis after a Traumatic Injury"

Article Title: Cutaneous Infection Caused by Gordonia amicalis after a Traumatic Injury

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.06681-11

Orange, opaque, dry, and nonhemolytic colonies grew on Trypticase soy agar supplemented with 5% sheep blood after incubation for 72 h.
Figure Legend Snippet: Orange, opaque, dry, and nonhemolytic colonies grew on Trypticase soy agar supplemented with 5% sheep blood after incubation for 72 h.

Techniques Used: Incubation

9) Product Images from "Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus"

Article Title: Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus

Journal: Immunology

doi: 10.1111/j.1365-2567.2008.02952.x

Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into trypticase soy broth in 10-fold serial dilutions of stock starting at 8 ×
Figure Legend Snippet: Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into trypticase soy broth in 10-fold serial dilutions of stock starting at 8 ×

Techniques Used:

10) Product Images from "Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns"

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.46.6.1837-1844.2002

Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.
Figure Legend Snippet: Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

Techniques Used: Incubation

11) Product Images from "Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression"

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression

Journal: Molecular & Cellular Proteomics : MCP

doi: 10.1074/mcp.RA118.001181

H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p
Figure Legend Snippet: H. pylori strain 7.13 colonizes gerbils and induces inflammation. A , Gastric tissue from uninfected (UI) and H. pylori -infected gerbils was homogenized and plated on selective trypticase soy agar plates with 5% sheep blood for isolation of H. pylori . Plates were incubated for 3–5 days, and colonization density was determined and expressed as log colony-forming units (CFU) per gram of gastric tissue. Each data point represents colonization density from an individual animal. B , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with Steiner stain to identify H. pylori topography within gastric tissue sections. White arrows designate regions with H. pylori colonization. C , Linear strips of gastric tissue, extending from the squamocolumnar junction through the proximal duodenum, were fixed in 10% neutral-buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. A pathologist (MBP), blinded to the treatment groups, assessed indices of inflammation. Severity of acute and chronic inflammation was graded 0–3 (absent (0), mild (1), moderate (2), or marked (3) inflammation) in both the gastric antrum and corpus. Each data point represents inflammation scores from an individual animal. Mann-Whitney U test was used to determine statistical significance between uninfected and infected groups. ***, p

Techniques Used: Infection, Isolation, Incubation, Staining, MANN-WHITNEY

12) Product Images from "Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine"

Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine

Journal: Infection and Immunity

doi: 10.1128/IAI.69.11.6823-6830.2001

Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and Trypticase soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was
Figure Legend Snippet: Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and Trypticase soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was

Techniques Used: Cell Culture, Staining

Related Articles

Functional Assay:

Article Title: Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System
Article Snippet: .. Supplementary Material Supplemental Figure 1: H. pylori strain 7.13 activation of TLR9 requires a functional cag T4SS The H. pylori cag + strain 7.13 or its isogenic mutants were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences) as described. .. TLR9-reporter or parental cells were cultured as described in and challenged with purified H. pylori strain 7.13 at an MOI of 100 for 24 hours.

Animal Model:

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns
Article Snippet: Paragraph title: Animal model. ... After 4 h, treated areas of the infected wound tissues were harvested aseptically, weighed, homogenized, serially diluted, and plated in triplicate on Trypticase soy agar with 5% sheep blood and Pseudomonas isolation agar (both from Becton Dickinson).

TCA Precipitation:

Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine
Article Snippet: A 5-ml sample was treated with 500 μl of 10% sodium dodecyl sulfate (SDS) and used for trichloroacetic acid precipitation of intracellular and extracellular proteins. .. Culture purity was verified by gram staining and plating on BGA and Trypticase soy agar (Becton-Dickinson).

In Vitro:

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: .. Wild-type carcinogenic c ag + H. pylori strain 7.13, a 7.13 isogenic cagE − ( cag secretion system ATPase) mutant, or a 7.13 isogenic cagA − ( cag secretion system effector protein) mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage. .. Wild-type cag + strain PMSS1 or its PMSS1 cagE − isogenic mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage.

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: .. Wild-type cag + strain PMSS1 or its PMSS1 cagE − isogenic mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage. .. Isogenic mutants were also cultured on Brucella agar (BD Biosciences) plates containing 20 μg/ml kanamycin (Sigma) to confirm presence of the kanamycin antibiotic resistance cassette.

Concentration Assay:

Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine
Article Snippet: The cells were resuspended in 10 ml of distilled water, disrupted by nitrogen cavitation with a nitrogen bomb (Fike Metal Products, Blue Springs, Mo.), and centrifuged as before, and the supernatant was analyzed for internal SO4 2− concentration. .. Culture purity was verified by gram staining and plating on BGA and Trypticase soy agar (Becton-Dickinson).

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns
Article Snippet: Group 1 received 1, 3, or 6 mg/kg of synthetic novispirin G10 ( n = 16 at each concentration). .. After 4 h, treated areas of the infected wound tissues were harvested aseptically, weighed, homogenized, serially diluted, and plated in triplicate on Trypticase soy agar with 5% sheep blood and Pseudomonas isolation agar (both from Becton Dickinson).

Mutagenesis:

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: .. Wild-type carcinogenic c ag + H. pylori strain 7.13, a 7.13 isogenic cagE − ( cag secretion system ATPase) mutant, or a 7.13 isogenic cagA − ( cag secretion system effector protein) mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage. .. Wild-type cag + strain PMSS1 or its PMSS1 cagE − isogenic mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage.

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: Wild-type carcinogenic cag + H. pylori strain 7.13 was minimally passaged on trypticase soy agar plates with 5% sheep blood (BD Biosciences, San Jose, CA) and in Brucella broth (BD Biosciences) supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, Flowery Branch, GA) for sixteen hours at 37 °C with 5% CO2 . .. Gerbils were orogastrically challenged with sterile Brucella broth (negative control), wild-type cag + H. pylori strain 7.13, or a cagE − isogenic mutant, and gerbils were euthanized 6 weeks post-challenge, as previously described ( ).

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: .. Wild-type cag + strain PMSS1 or its PMSS1 cagE − isogenic mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage. .. Isogenic mutants were also cultured on Brucella agar (BD Biosciences) plates containing 20 μg/ml kanamycin (Sigma) to confirm presence of the kanamycin antibiotic resistance cassette.

Isolation:

Article Title: Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus
Article Snippet: .. Staphylococcus aureus (SA) D30 which has been extensively characterized, was originally isolated from the anterior nares of a healthy donor, and served as the carrier strain in the experiments herein., , Staphylococcus aureus 930918-3, (from Ian Holder, Shriners Burn Hospital, Cincinnati, OH) served as the non-carrier strain of S. aureus in these experiments and S. epidermidis was kindly provided by Dr Robert I. Lehrer, University of California (Los Angeles)., Other strains of S. aureus used in the biofilm experiments included S. aureus 502A (an intermittent carrier used in intervention studies in the 1960s), S. aureus D20, S. aureus D98 and S. aureus D85 (designated carrier strains)., , Bacteria were cultivated on trypticase soy agar (TSA, Bacto™; Becton Dickinson and Company, Sparks, MD) and subcultured in tryptic soy broth (TSB; Bacto™) from which stocks were prepared. .. For all experiments, snap-frozen (−80°) stocks were thawed rapidly and cultured at 37°.

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model
Article Snippet: .. The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD). ..

Article Title: Chromobacterium violaceum infections in 13 non-human primates
Article Snippet: .. Bacterial isolation was performed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood and MacConkey II agar of BBL prepared plated media (BD Diagnostic, Sparks, MD, USA). .. The biochemical tests and characterizations of isolated bacteria were assayed by API-20E kit (BioMerieux, Durham, NC, USA).

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns
Article Snippet: .. After 4 h, treated areas of the infected wound tissues were harvested aseptically, weighed, homogenized, serially diluted, and plated in triplicate on Trypticase soy agar with 5% sheep blood and Pseudomonas isolation agar (both from Becton Dickinson). ..

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model
Article Snippet: .. The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD). ..

Polymerase Chain Reaction:

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model
Article Snippet: The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD). .. Fifty microliters from each of the four biopsy homogenates per animals were pooled for analysis by PCR using P. aeruginosa -specific and universal primers.

Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model
Article Snippet: The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD). .. Fifty microliters from each of the four biopsy homogenates per animals were pooled for analysis by PCR using P. aeruginosa -specific and universal primers.

Negative Control:

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: Wild-type carcinogenic cag + H. pylori strain 7.13 was minimally passaged on trypticase soy agar plates with 5% sheep blood (BD Biosciences, San Jose, CA) and in Brucella broth (BD Biosciences) supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, Flowery Branch, GA) for sixteen hours at 37 °C with 5% CO2 . .. Gerbils were orogastrically challenged with sterile Brucella broth (negative control), wild-type cag + H. pylori strain 7.13, or a cagE − isogenic mutant, and gerbils were euthanized 6 weeks post-challenge, as previously described ( ).

Cell Culture:

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: .. Wild-type carcinogenic c ag + H. pylori strain 7.13, a 7.13 isogenic cagE − ( cag secretion system ATPase) mutant, or a 7.13 isogenic cagA − ( cag secretion system effector protein) mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage. .. Wild-type cag + strain PMSS1 or its PMSS1 cagE − isogenic mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage.

Article Title: Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus
Article Snippet: Staphylococcus aureus (SA) D30 which has been extensively characterized, was originally isolated from the anterior nares of a healthy donor, and served as the carrier strain in the experiments herein., , Staphylococcus aureus 930918-3, (from Ian Holder, Shriners Burn Hospital, Cincinnati, OH) served as the non-carrier strain of S. aureus in these experiments and S. epidermidis was kindly provided by Dr Robert I. Lehrer, University of California (Los Angeles)., Other strains of S. aureus used in the biofilm experiments included S. aureus 502A (an intermittent carrier used in intervention studies in the 1960s), S. aureus D20, S. aureus D98 and S. aureus D85 (designated carrier strains)., , Bacteria were cultivated on trypticase soy agar (TSA, Bacto™; Becton Dickinson and Company, Sparks, MD) and subcultured in tryptic soy broth (TSB; Bacto™) from which stocks were prepared. .. For all experiments, snap-frozen (−80°) stocks were thawed rapidly and cultured at 37°.

Article Title: Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System
Article Snippet: Supplementary Material Supplemental Figure 1: H. pylori strain 7.13 activation of TLR9 requires a functional cag T4SS The H. pylori cag + strain 7.13 or its isogenic mutants were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences) as described. .. TLR9-reporter or parental cells were cultured as described in and challenged with purified H. pylori strain 7.13 at an MOI of 100 for 24 hours.

Article Title: Carcinogenic Helicobacter pylori Strains Selectively Dysregulate the In Vivo Gastric Proteome, Which May Be Associated with Stomach Cancer Progression
Article Snippet: .. Wild-type cag + strain PMSS1 or its PMSS1 cagE − isogenic mutant were cultured on trypticase soy agar with 5% sheep blood agar plates (BD Biosciences) for in vitro passage. .. Isogenic mutants were also cultured on Brucella agar (BD Biosciences) plates containing 20 μg/ml kanamycin (Sigma) to confirm presence of the kanamycin antibiotic resistance cassette.

Sampling:

Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine
Article Snippet: Paragraph title: Fermentation sampling. ... Culture purity was verified by gram staining and plating on BGA and Trypticase soy agar (Becton-Dickinson).

Activation Assay:

Article Title: Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System
Article Snippet: .. Supplementary Material Supplemental Figure 1: H. pylori strain 7.13 activation of TLR9 requires a functional cag T4SS The H. pylori cag + strain 7.13 or its isogenic mutants were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences) as described. .. TLR9-reporter or parental cells were cultured as described in and challenged with purified H. pylori strain 7.13 at an MOI of 100 for 24 hours.

Incubation:

Article Title: Cutaneous Infection Caused by Gordonia amicalis after a Traumatic Injury
Article Snippet: .. Cultures on Trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Sparks, MD) grew small numbers of slightly orange and dry colonies and Gram-positive coryneform bacilli with rudimentary branching and weakly acid-fast bacilli after incubation for 4 days. .. The growth on chocolate agar (Becton Dickinson) and CDC blood agar plate (Becton Dickinson) was negative.

Article Title: Rinsability of Orthophthalaldehyde from Endoscopes
Article Snippet: A culture of Staphylococcus aureus, American Type Culture Collection no. 6538, was spread over the surface of trypticase soy agar (Becton Dickinson) in a 100 × 15 mm plastic petri dish (Fox Scientific). .. The disinfected and rinsed 2.0 cm × 1.0 cm pieces of endoscope materials were placed individually onto the surface of the bacteria-seeded agar in petri plates, and the plates were incubated for 48 ± 8 hrs at 35 ± 2°C to form a confluent “lawn” of bacteria.

Inhibition:

Article Title: Rinsability of Orthophthalaldehyde from Endoscopes
Article Snippet: A culture of Staphylococcus aureus, American Type Culture Collection no. 6538, was spread over the surface of trypticase soy agar (Becton Dickinson) in a 100 × 15 mm plastic petri dish (Fox Scientific). .. Zones of inhibition (ZOI) where the bacteria could not grow were measured around the endoscope materials in mm from side to side.

Infection:

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns
Article Snippet: .. After 4 h, treated areas of the infected wound tissues were harvested aseptically, weighed, homogenized, serially diluted, and plated in triplicate on Trypticase soy agar with 5% sheep blood and Pseudomonas isolation agar (both from Becton Dickinson). ..

Modification:

Article Title: Chromobacterium violaceum infections in 13 non-human primates
Article Snippet: .. Bacterial isolation was performed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood and MacConkey II agar of BBL prepared plated media (BD Diagnostic, Sparks, MD, USA). .. The biochemical tests and characterizations of isolated bacteria were assayed by API-20E kit (BioMerieux, Durham, NC, USA).

Staining:

Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine
Article Snippet: .. Culture purity was verified by gram staining and plating on BGA and Trypticase soy agar (Becton-Dickinson). ..

Article Title: Chromobacterium violaceum infections in 13 non-human primates
Article Snippet: Selected sections were also stained with Gram's stain. .. Bacterial isolation was performed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood and MacConkey II agar of BBL prepared plated media (BD Diagnostic, Sparks, MD, USA).

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns
Article Snippet: After 4 h, treated areas of the infected wound tissues were harvested aseptically, weighed, homogenized, serially diluted, and plated in triplicate on Trypticase soy agar with 5% sheep blood and Pseudomonas isolation agar (both from Becton Dickinson). .. In addition, 5-μm-thick paraffin tissue sections of skin were stained (hematoxylin/eosin) to evaluate the morphology of the burn wound after treatment with novispirin G10 or vehicle control or the no-treatment control.

Injection:

Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns
Article Snippet: Treatment was applied by one intradermal injection of 500 μl with a 30-gauge needle to both marked center portions (4 cm2 each) of the burned and infected area. .. After 4 h, treated areas of the infected wound tissues were harvested aseptically, weighed, homogenized, serially diluted, and plated in triplicate on Trypticase soy agar with 5% sheep blood and Pseudomonas isolation agar (both from Becton Dickinson).

Purification:

Article Title: Pathogenic Helicobacter pylori Strains Translocate DNA and Activate TLR9 via the Cancer-Associated cag Type IV Secretion System
Article Snippet: Supplementary Material Supplemental Figure 1: H. pylori strain 7.13 activation of TLR9 requires a functional cag T4SS The H. pylori cag + strain 7.13 or its isogenic mutants were maintained on trypticase soy agar plates supplemented with 5% sheep blood (BD Biosciences) as described. .. TLR9-reporter or parental cells were cultured as described in and challenged with purified H. pylori strain 7.13 at an MOI of 100 for 24 hours.

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    Becton Dickinson trypticase soy agar
    Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or <t>Trypticase</t> soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .
    Trypticase Soy Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Becton Dickinson
    Average 99 stars, based on 13 article reviews
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    trypticase soy agar - by Bioz Stars, 2020-04
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    91
    Becton Dickinson tryptic soy broth agar growth medium
    <t>Growth</t> kinetics of B. subtilis wild-type and mutant strains after exposure to root exudates elicited by 2,3-butanediol. Initial cell culture concentrations were OD 600 = 0.02 and data are shown as log-normal plots. <t>Tryptic</t> <t>soy</t> <t>broth</t> supplemented with root exudate at a 1:1 ratio was applied to pepper roots. Aliquots (150 μl) from each culture were transferred to 100 wells of a Bioscreen plate. Plates were incubated in a Bioscreen C (Fluoroskan; Labsystems, Helsinki, Finland) with shaking at 30°C for ~4 days. The OD 600 of each well was measured every 15 min. 2.3B = 2,3-butanediol; 23BE = root exudate collected from 2,3-butanediol-treated root system; 168 = B. subtilis 168; BSIP 1174 = B. subtilis BSIP 1174 (non-producer); BSIP 1171 = B. subtilis BSIP 1174 (overproducer); Pf-5 = Pseudomonas protegens Pf-5; GMI1000 = Ralstonia solanacearum GMI1000; M = MS broth; ME = MS broth amended with root exudate without treatment. (A) Schematic of protocol to extract root exudates after 2,3-butanediol application. (B) Growth kinetics of the three strains in control TSB <t>medium.</t> The figure indicate background expression of three strains 168, 2,3-B(++), and 2,3-B(-). (C) Growth of bacterial strains 168, 2,3-B(++), and 2,3-B(-) after treatment with 2,3-butanedol alone (referred to as 2,3B) or 2,3-butanediol-elicited root exudate (referred to as 2,3BE). (D) Growth kinetics of P. protegens Pf-5, Ralstonia solanacearum GMI1000, and E. coli . P. protegens Pf-5 is a non-pathogenic saprophyte that inhabits soil, water, and plant surface environments. Growth of P. protegens Pf-5 was not inhibited by 2,3-butanediol-elicited exudate. Growth of GMI1000, a soil-borne bacterial wilt pathogen, was inhibited by root exudates. E. coli was included as a bacterial control. Data shown are mean ± SEM of triplicate experiments.
    Tryptic Soy Broth Agar Growth Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tryptic soy broth agar growth medium/product/Becton Dickinson
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tryptic soy broth agar growth medium - by Bioz Stars, 2020-04
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    Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

    Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

    doi: 10.1093/jbcr/iry043

    Figure Lengend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Article Snippet: The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD).

    Techniques: Infection, Serial Dilution, Isolation

    Chromobacterium violaceum isolation (From the liver of Case No. 3). Distinctive violet pigmented colonies were developed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood media after anaerobic incubation for 24 hours at 37°C.

    Journal: Journal of medical primatology

    Article Title: Chromobacterium violaceum infections in 13 non-human primates

    doi: 10.1111/j.1600-0684.2011.00529.x

    Figure Lengend Snippet: Chromobacterium violaceum isolation (From the liver of Case No. 3). Distinctive violet pigmented colonies were developed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood media after anaerobic incubation for 24 hours at 37°C.

    Article Snippet: Bacterial isolation was performed on Trypticase Soy Agar, Modified (TSA II) with 5% sheep blood and MacConkey II agar of BBL prepared plated media (BD Diagnostic, Sparks, MD, USA).

    Techniques: Isolation, Modification, Incubation

    Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

    Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

    doi: 10.1093/jbcr/iry043

    Figure Lengend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Article Snippet: The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD).

    Techniques: Infection, Serial Dilution, Isolation

    Growth kinetics of B. subtilis wild-type and mutant strains after exposure to root exudates elicited by 2,3-butanediol. Initial cell culture concentrations were OD 600 = 0.02 and data are shown as log-normal plots. Tryptic soy broth supplemented with root exudate at a 1:1 ratio was applied to pepper roots. Aliquots (150 μl) from each culture were transferred to 100 wells of a Bioscreen plate. Plates were incubated in a Bioscreen C (Fluoroskan; Labsystems, Helsinki, Finland) with shaking at 30°C for ~4 days. The OD 600 of each well was measured every 15 min. 2.3B = 2,3-butanediol; 23BE = root exudate collected from 2,3-butanediol-treated root system; 168 = B. subtilis 168; BSIP 1174 = B. subtilis BSIP 1174 (non-producer); BSIP 1171 = B. subtilis BSIP 1174 (overproducer); Pf-5 = Pseudomonas protegens Pf-5; GMI1000 = Ralstonia solanacearum GMI1000; M = MS broth; ME = MS broth amended with root exudate without treatment. (A) Schematic of protocol to extract root exudates after 2,3-butanediol application. (B) Growth kinetics of the three strains in control TSB medium. The figure indicate background expression of three strains 168, 2,3-B(++), and 2,3-B(-). (C) Growth of bacterial strains 168, 2,3-B(++), and 2,3-B(-) after treatment with 2,3-butanedol alone (referred to as 2,3B) or 2,3-butanediol-elicited root exudate (referred to as 2,3BE). (D) Growth kinetics of P. protegens Pf-5, Ralstonia solanacearum GMI1000, and E. coli . P. protegens Pf-5 is a non-pathogenic saprophyte that inhabits soil, water, and plant surface environments. Growth of P. protegens Pf-5 was not inhibited by 2,3-butanediol-elicited exudate. Growth of GMI1000, a soil-borne bacterial wilt pathogen, was inhibited by root exudates. E. coli was included as a bacterial control. Data shown are mean ± SEM of triplicate experiments.

    Journal: Frontiers in Microbiology

    Article Title: Impact of a Bacterial Volatile 2,3-Butanediol on Bacillus subtilis Rhizosphere Robustness

    doi: 10.3389/fmicb.2016.00993

    Figure Lengend Snippet: Growth kinetics of B. subtilis wild-type and mutant strains after exposure to root exudates elicited by 2,3-butanediol. Initial cell culture concentrations were OD 600 = 0.02 and data are shown as log-normal plots. Tryptic soy broth supplemented with root exudate at a 1:1 ratio was applied to pepper roots. Aliquots (150 μl) from each culture were transferred to 100 wells of a Bioscreen plate. Plates were incubated in a Bioscreen C (Fluoroskan; Labsystems, Helsinki, Finland) with shaking at 30°C for ~4 days. The OD 600 of each well was measured every 15 min. 2.3B = 2,3-butanediol; 23BE = root exudate collected from 2,3-butanediol-treated root system; 168 = B. subtilis 168; BSIP 1174 = B. subtilis BSIP 1174 (non-producer); BSIP 1171 = B. subtilis BSIP 1174 (overproducer); Pf-5 = Pseudomonas protegens Pf-5; GMI1000 = Ralstonia solanacearum GMI1000; M = MS broth; ME = MS broth amended with root exudate without treatment. (A) Schematic of protocol to extract root exudates after 2,3-butanediol application. (B) Growth kinetics of the three strains in control TSB medium. The figure indicate background expression of three strains 168, 2,3-B(++), and 2,3-B(-). (C) Growth of bacterial strains 168, 2,3-B(++), and 2,3-B(-) after treatment with 2,3-butanedol alone (referred to as 2,3B) or 2,3-butanediol-elicited root exudate (referred to as 2,3BE). (D) Growth kinetics of P. protegens Pf-5, Ralstonia solanacearum GMI1000, and E. coli . P. protegens Pf-5 is a non-pathogenic saprophyte that inhabits soil, water, and plant surface environments. Growth of P. protegens Pf-5 was not inhibited by 2,3-butanediol-elicited exudate. Growth of GMI1000, a soil-borne bacterial wilt pathogen, was inhibited by root exudates. E. coli was included as a bacterial control. Data shown are mean ± SEM of triplicate experiments.

    Article Snippet: Bacterial strains were isolated from plant roots using specific antibiotics in the tryptic soy broth agar growth medium (TSA, BactoTM, BD, Sparks, MD, USA): 50 μg/ml rifampicin for strain 168, 10 μg/ml spectinomycin for 2,3-B(-), and 10 μg/ml spectinomycin plus 5 μg/ml chloramphenicol for 2,3-B(++).

    Techniques: Mutagenesis, Cell Culture, Incubation, Mass Spectrometry, Expressing