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Becton Dickinson trypticase soy agar tsa
Trypticase Soy Agar Tsa, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy agar tsa/product/Becton Dickinson
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trypticase soy agar tsa - by Bioz Stars, 2020-04
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Diagnostic Assay:

Article Title: Maresin 1 Biosynthesis and Proresolving Anti-infective Functions with Human-Localized Aggressive Periodontitis Leukocytes
Article Snippet: Porphyromonas gingivalis (A7436) was cultured on 2% Trypticase soy agar (TSA) supplemented with 2.6% brain heart infusion agar (BD BBL), 1% (wt/vol) yeast extract (BD Bacto), 5% defibrinated sheep red blood cells (Northeast Laboratory Services), 5 μg/ml of hemin, and 0.5 μg/ml vitamin K (Sigma-Aldrich). .. Porphyromonas gingivalis (A7436) was cultured on 2% Trypticase soy agar (TSA) supplemented with 2.6% brain heart infusion agar (BD BBL), 1% (wt/vol) yeast extract (BD Bacto), 5% defibrinated sheep red blood cells (Northeast Laboratory Services), 5 μg/ml of hemin, and 0.5 μg/ml vitamin K (Sigma-Aldrich).

Amplification:

Article Title: Relationship between Ceftolozane-Tazobactam Exposure and Drug Resistance Amplification in a Hollow-Fiber Infection Model
Article Snippet: Paragraph title: Resistance amplification prevention studies. ... In these studies, the initial challenge isolate inoculum was prepared from an overnight culture grown on Trypticase soy agar (TSA) plus 5% sheep blood (BD Laboratories) at 35°C.

In Vitro:

Article Title: New Insights into Clostridium difficile (CD) Infection in Latin America: Novel Description of Toxigenic Profiles of Diarrhea-Associated to CD in Bogotá, Colombia
Article Snippet: Paragraph title: Detecting CDI by in vitro culture ... Because a possible co-existence of different CD genotypes has been reported in a sample simultaneously (Tanner et al., ), from 1 to 7 colonies (depending on the amount of colony-forming units recovered for each sample during the initial step in SCM), were extended on trypticase soy agar (TSA) with 5% sheep blood (Becton Dickinson), and subsequently incubated under aforementioned conditions.

Mutagenesis:

Article Title: Helicobacter pylori Urease Activity is Influenced by Ferric Uptake Regulator
Article Snippet: An ATCC 43504 fur deletion mutant was constructed by allelic exchange using a kanamycin resistance gene as described below. .. H. pylori was grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on trypticase soy agar (TSA) plates supplemented with 5% sheep blood (Becton Dickinson, Cockeysville, MD, USA) or in brain heart infusion (BHI) medium (Difco Laboratories, Detroit, MI, USA) supplemented with 7% horse serum (Gibco BRL-Life Technologies Inc., Carlsbad, CA, USA) and 0.25% yeast extract (Difco Laboratories).

Isolation:

Article Title: Bacillus Spore Inactivation Methods Affect Detection Assays
Article Snippet: .. Frozen glycerol stock cultures were streaked for isolation onto Trypticase soy agar (TSA) plus 5% sheep blood (BD Biosciences, Cockeysville, Md.). .. Isolated colonies were streaked again for isolation onto TSA + 5% sheep blood and incubated at 37°C for approximately 6 h or until visible growth was evident.

Co-Culture Assay:

Article Title: Effects of Lactobacillus rhamnosus and Lactobacillus acidophilus on bacterial vaginal pathogens
Article Snippet: G. vaginalis and A. vaginae , obtained from University of Göteborg (Sweden) were cultivated anaerobically using the GasPak anaerobic envelope system (Becton Dickinson, Erembodegem, Belgium) at 37°C on Trypticase Soy Agar (TSA) + 5% sheep blood (Becton Dickinson). .. The capability of L. acidophilus GLA-14 and L. rhamnosus HN001 to interfere with the growth of the different pathogens was evaluated by a liquid co-culture assay in anaerobiosis or in aerobiosis, depending on the particular bacterial strain used.

Colony Assay:

Article Title: New Insights into Clostridium difficile (CD) Infection in Latin America: Novel Description of Toxigenic Profiles of Diarrhea-Associated to CD in Bogotá, Colombia
Article Snippet: SCM results were confirmed by verification by colony screening (VCS) (Figure ). .. Because a possible co-existence of different CD genotypes has been reported in a sample simultaneously (Tanner et al., ), from 1 to 7 colonies (depending on the amount of colony-forming units recovered for each sample during the initial step in SCM), were extended on trypticase soy agar (TSA) with 5% sheep blood (Becton Dickinson), and subsequently incubated under aforementioned conditions.

Concentration Assay:

Article Title: Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards
Article Snippet: Determination of colony forming units (CFU) was done by drop-plating (Herigstad et al. ) ten-fold dilution series in triplicate onto trypticase soy agar (TSA) (211042, BD, Diagnostic Systems, Sparks, MD, USA). .. DNA concentration was measured using QUBIT dsDNA BR Assay (Q32850, Life Technologies, Grand Island, NY, USA) and adjusted to 1 ng/μL.

Article Title: Relationship between Ceftolozane-Tazobactam Exposure and Drug Resistance Amplification in a Hollow-Fiber Infection Model
Article Snippet: In these studies, the initial challenge isolate inoculum was prepared from an overnight culture grown on Trypticase soy agar (TSA) plus 5% sheep blood (BD Laboratories) at 35°C. .. The bacterial concentration within the flask of MH broth was determined by measuring the optical density and using a previously confirmed growth curve for the challenge isolate.

Article Title: Are We Biologically Safe with Snow Precipitation? A Case Study in Beijing
Article Snippet: For the snow samples (pooled together) collected on each occurrence on a particular date, 80 µl of the original suspesion (diluted 10 times) was pipetted onto on a Trypticase Soy Agar (TSA) (Becton, Dickson and Company, Sparks, MD) plate for bacteria culturing. .. The colony forming units (CFUs) were manually counted and the bacterial concentration was calculated as CFU/ml of melted snow suspenison.

Incubation:

Article Title: Antibiotic susceptibility of Atopobium vaginae
Article Snippet: Strains were cultivated anaerobically using the GasPak anaerobic envelope system (Becton Dickinson, Erembodegem, Belgium) at 37°C on Trypticase Soy Agar (TSA) + 5 % sheep blood (Becton Dickinson). .. Before inoculating the plates and testing for metronidazole, plates were anaerobically incubated during 24 h. After application of the epsilometer, test plates were anaerobically incubated at 37°C.

Article Title: CRTH2 Is A Critical Regulator of Neutrophil Migration and Resistance to Polymicrobial Sepsis
Article Snippet: .. Equal volumes of each dilution was applied to trypticase soy agar (TSA) plates with 5% sheep blood (BD™ Trypticase™ Soy Agar II with 5% Sheep Blood: BD Diagnostics, Sparks, MD) and were incubated overnight at 37°C. .. After overnight incubation, the number of bacteria colonies was counted and was expressed as colony-forming units (CFU) per indicated volume of peritoneal lavage fluids.

Article Title: Bacillus Spore Inactivation Methods Affect Detection Assays
Article Snippet: Frozen glycerol stock cultures were streaked for isolation onto Trypticase soy agar (TSA) plus 5% sheep blood (BD Biosciences, Cockeysville, Md.). .. Isolated colonies were streaked again for isolation onto TSA + 5% sheep blood and incubated at 37°C for approximately 6 h or until visible growth was evident.

Article Title: Effects of Lactobacillus rhamnosus and Lactobacillus acidophilus on bacterial vaginal pathogens
Article Snippet: Before the experiments, each strain was transferred from the frozen stock culture to MRS (De Man Rugosa Sharpe) broth incubated at 37°C under non-agitated aerobic conditions. .. G. vaginalis and A. vaginae , obtained from University of Göteborg (Sweden) were cultivated anaerobically using the GasPak anaerobic envelope system (Becton Dickinson, Erembodegem, Belgium) at 37°C on Trypticase Soy Agar (TSA) + 5% sheep blood (Becton Dickinson).

Article Title: New Insights into Clostridium difficile (CD) Infection in Latin America: Novel Description of Toxigenic Profiles of Diarrhea-Associated to CD in Bogotá, Colombia
Article Snippet: .. Because a possible co-existence of different CD genotypes has been reported in a sample simultaneously (Tanner et al., ), from 1 to 7 colonies (depending on the amount of colony-forming units recovered for each sample during the initial step in SCM), were extended on trypticase soy agar (TSA) with 5% sheep blood (Becton Dickinson), and subsequently incubated under aforementioned conditions. .. VCS was completed by microscopic inspection by routine interpretation by Gram staining, using a smear of the colony on a slide with saline solution.

Cell Culture:

Article Title: Are We Biologically Safe with Snow Precipitation? A Case Study in Beijing
Article Snippet: For the snow samples (pooled together) collected on each occurrence on a particular date, 80 µl of the original suspesion (diluted 10 times) was pipetted onto on a Trypticase Soy Agar (TSA) (Becton, Dickson and Company, Sparks, MD) plate for bacteria culturing. .. Bacterial and fungal aerosols are often cultured at 26°C and 37°C, while use of 4°C could readily activate those bioaerosols acting as ice nucleators.

Article Title: Maresin 1 Biosynthesis and Proresolving Anti-infective Functions with Human-Localized Aggressive Periodontitis Leukocytes
Article Snippet: .. Porphyromonas gingivalis (A7436) was cultured on 2% Trypticase soy agar (TSA) supplemented with 2.6% brain heart infusion agar (BD BBL), 1% (wt/vol) yeast extract (BD Bacto), 5% defibrinated sheep red blood cells (Northeast Laboratory Services), 5 μg/ml of hemin, and 0.5 μg/ml vitamin K (Sigma-Aldrich). .. Aggregatibacter actinomycetemcomitans (ATCC Y4), Fusobacterium nucleatum subsp . nucleatum (ATCC 25586), and Streptococcus intermedius (ATCC 27335) were grown on TSA plates (BD Diagnostic Systems).

Article Title: Effects of Lactobacillus rhamnosus and Lactobacillus acidophilus on bacterial vaginal pathogens
Article Snippet: G. vaginalis and A. vaginae , obtained from University of Göteborg (Sweden) were cultivated anaerobically using the GasPak anaerobic envelope system (Becton Dickinson, Erembodegem, Belgium) at 37°C on Trypticase Soy Agar (TSA) + 5% sheep blood (Becton Dickinson). .. UPEC E. coli CFT073 (O6:K2:H1, ATCC700928) and S. aureus (ATCC29213) were cultured in Luria Bertani (LB) and Tryptone Soy (TSB) broths, respectively.

Activity Assay:

Article Title: Relationship between Ceftolozane-Tazobactam Exposure and Drug Resistance Amplification in a Hollow-Fiber Infection Model
Article Snippet: In each study, control regimens included a no-treatment growth control, ceftolozane infused at 375 mg over 1 h every 8 h (demonstrating limited bactericidal activity), and piperacillin-tazobactam infused at 4.5 g over 0.5 h every 6 h. A tazobactam control arm was omitted because this compound has been shown to have no therapeutic effect on the study isolate ( ). .. In these studies, the initial challenge isolate inoculum was prepared from an overnight culture grown on Trypticase soy agar (TSA) plus 5% sheep blood (BD Laboratories) at 35°C.

Microscopy:

Article Title: Bacillus Spore Inactivation Methods Affect Detection Assays
Article Snippet: Frozen glycerol stock cultures were streaked for isolation onto Trypticase soy agar (TSA) plus 5% sheep blood (BD Biosciences, Cockeysville, Md.). .. These plates were incubated at 37°C and monitored for spore formation by phase-contrast microscopy with the criteria that vegetative cells are dark and rod shaped and often present as chains of cells, while spores are highly refractile and appear as smaller, bright ovals either inside of vegetative cells or free floating.

Staining:

Article Title: New Insights into Clostridium difficile (CD) Infection in Latin America: Novel Description of Toxigenic Profiles of Diarrhea-Associated to CD in Bogotá, Colombia
Article Snippet: Because a possible co-existence of different CD genotypes has been reported in a sample simultaneously (Tanner et al., ), from 1 to 7 colonies (depending on the amount of colony-forming units recovered for each sample during the initial step in SCM), were extended on trypticase soy agar (TSA) with 5% sheep blood (Becton Dickinson), and subsequently incubated under aforementioned conditions. .. VCS was completed by microscopic inspection by routine interpretation by Gram staining, using a smear of the colony on a slide with saline solution.

Sequencing:

Article Title: Antibiotic susceptibility of Atopobium vaginae
Article Snippet: The other strains were obtained from studies by cultivating vaginal samples (with informed consent) and identified by tDNA-PCR and 16S rRNA gene sequencing [ ]. .. Strains were cultivated anaerobically using the GasPak anaerobic envelope system (Becton Dickinson, Erembodegem, Belgium) at 37°C on Trypticase Soy Agar (TSA) + 5 % sheep blood (Becton Dickinson).

Construct:

Article Title: Helicobacter pylori Urease Activity is Influenced by Ferric Uptake Regulator
Article Snippet: An ATCC 43504 fur deletion mutant was constructed by allelic exchange using a kanamycin resistance gene as described below. .. H. pylori was grown under microaerobic conditions (5% O2 , 10% CO2 , 85% N2 ) either on trypticase soy agar (TSA) plates supplemented with 5% sheep blood (Becton Dickinson, Cockeysville, MD, USA) or in brain heart infusion (BHI) medium (Difco Laboratories, Detroit, MI, USA) supplemented with 7% horse serum (Gibco BRL-Life Technologies Inc., Carlsbad, CA, USA) and 0.25% yeast extract (Difco Laboratories).

other:

Article Title: Strategy for Cross-Protection among Shigella flexneri Serotypes
Article Snippet: Wild-type Shigella strains were grown on Trypticase soy agar (TSA) (BBL Becton Dickinson, Cockeysville, Md.) with 0.01% Congo red dye (CR) (Sigma, St. Louis, Mo.) (TSA-CR).

Article Title: The Microbiota of Freshwater Fish and Freshwater Niches Contain Omega-3 Fatty Acid-Producing Shewanella Species
Article Snippet: Cells were grown in Trypticase soy agar (TSA) (BD BBL) at 25°C, resuspended in 0.85% NaCl, and inoculated into the strips per the manufacturer's instruction.

Plasmid Preparation:

Article Title: Bacillus Spore Inactivation Methods Affect Detection Assays
Article Snippet: Inactivation and biodetection protocols were conducted using B. anthracis NNR1 (pX01+ pX02− ), B. anthracis ΔAmes (pX01− pX02+ ), B. anthracis ΔSterne (plasmid free), and two negative controls, Bacillus subtilis strain 1031 and Bacillus thuringiensis subsp. kurstaki. .. Frozen glycerol stock cultures were streaked for isolation onto Trypticase soy agar (TSA) plus 5% sheep blood (BD Biosciences, Cockeysville, Md.).

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  • 91
    Becton Dickinson tryptic soy broth agar growth medium
    <t>Growth</t> kinetics of B. subtilis wild-type and mutant strains after exposure to root exudates elicited by 2,3-butanediol. Initial cell culture concentrations were OD 600 = 0.02 and data are shown as log-normal plots. <t>Tryptic</t> <t>soy</t> <t>broth</t> supplemented with root exudate at a 1:1 ratio was applied to pepper roots. Aliquots (150 μl) from each culture were transferred to 100 wells of a Bioscreen plate. Plates were incubated in a Bioscreen C (Fluoroskan; Labsystems, Helsinki, Finland) with shaking at 30°C for ~4 days. The OD 600 of each well was measured every 15 min. 2.3B = 2,3-butanediol; 23BE = root exudate collected from 2,3-butanediol-treated root system; 168 = B. subtilis 168; BSIP 1174 = B. subtilis BSIP 1174 (non-producer); BSIP 1171 = B. subtilis BSIP 1174 (overproducer); Pf-5 = Pseudomonas protegens Pf-5; GMI1000 = Ralstonia solanacearum GMI1000; M = MS broth; ME = MS broth amended with root exudate without treatment. (A) Schematic of protocol to extract root exudates after 2,3-butanediol application. (B) Growth kinetics of the three strains in control TSB <t>medium.</t> The figure indicate background expression of three strains 168, 2,3-B(++), and 2,3-B(-). (C) Growth of bacterial strains 168, 2,3-B(++), and 2,3-B(-) after treatment with 2,3-butanedol alone (referred to as 2,3B) or 2,3-butanediol-elicited root exudate (referred to as 2,3BE). (D) Growth kinetics of P. protegens Pf-5, Ralstonia solanacearum GMI1000, and E. coli . P. protegens Pf-5 is a non-pathogenic saprophyte that inhabits soil, water, and plant surface environments. Growth of P. protegens Pf-5 was not inhibited by 2,3-butanediol-elicited exudate. Growth of GMI1000, a soil-borne bacterial wilt pathogen, was inhibited by root exudates. E. coli was included as a bacterial control. Data shown are mean ± SEM of triplicate experiments.
    Tryptic Soy Broth Agar Growth Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tryptic soy broth agar growth medium/product/Becton Dickinson
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tryptic soy broth agar growth medium - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    99
    Becton Dickinson trypticase soy agar
    Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into <t>trypticase</t> soy broth in 10-fold serial dilutions of stock starting at 8 ×
    Trypticase Soy Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Becton Dickinson
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Becton Dickinson trypticase soy agar tsa
    Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into <t>trypticase</t> soy broth in 10-fold serial dilutions of stock starting at 8 ×
    Trypticase Soy Agar Tsa, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar tsa/product/Becton Dickinson
    Average 94 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar tsa - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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    Growth kinetics of B. subtilis wild-type and mutant strains after exposure to root exudates elicited by 2,3-butanediol. Initial cell culture concentrations were OD 600 = 0.02 and data are shown as log-normal plots. Tryptic soy broth supplemented with root exudate at a 1:1 ratio was applied to pepper roots. Aliquots (150 μl) from each culture were transferred to 100 wells of a Bioscreen plate. Plates were incubated in a Bioscreen C (Fluoroskan; Labsystems, Helsinki, Finland) with shaking at 30°C for ~4 days. The OD 600 of each well was measured every 15 min. 2.3B = 2,3-butanediol; 23BE = root exudate collected from 2,3-butanediol-treated root system; 168 = B. subtilis 168; BSIP 1174 = B. subtilis BSIP 1174 (non-producer); BSIP 1171 = B. subtilis BSIP 1174 (overproducer); Pf-5 = Pseudomonas protegens Pf-5; GMI1000 = Ralstonia solanacearum GMI1000; M = MS broth; ME = MS broth amended with root exudate without treatment. (A) Schematic of protocol to extract root exudates after 2,3-butanediol application. (B) Growth kinetics of the three strains in control TSB medium. The figure indicate background expression of three strains 168, 2,3-B(++), and 2,3-B(-). (C) Growth of bacterial strains 168, 2,3-B(++), and 2,3-B(-) after treatment with 2,3-butanedol alone (referred to as 2,3B) or 2,3-butanediol-elicited root exudate (referred to as 2,3BE). (D) Growth kinetics of P. protegens Pf-5, Ralstonia solanacearum GMI1000, and E. coli . P. protegens Pf-5 is a non-pathogenic saprophyte that inhabits soil, water, and plant surface environments. Growth of P. protegens Pf-5 was not inhibited by 2,3-butanediol-elicited exudate. Growth of GMI1000, a soil-borne bacterial wilt pathogen, was inhibited by root exudates. E. coli was included as a bacterial control. Data shown are mean ± SEM of triplicate experiments.

    Journal: Frontiers in Microbiology

    Article Title: Impact of a Bacterial Volatile 2,3-Butanediol on Bacillus subtilis Rhizosphere Robustness

    doi: 10.3389/fmicb.2016.00993

    Figure Lengend Snippet: Growth kinetics of B. subtilis wild-type and mutant strains after exposure to root exudates elicited by 2,3-butanediol. Initial cell culture concentrations were OD 600 = 0.02 and data are shown as log-normal plots. Tryptic soy broth supplemented with root exudate at a 1:1 ratio was applied to pepper roots. Aliquots (150 μl) from each culture were transferred to 100 wells of a Bioscreen plate. Plates were incubated in a Bioscreen C (Fluoroskan; Labsystems, Helsinki, Finland) with shaking at 30°C for ~4 days. The OD 600 of each well was measured every 15 min. 2.3B = 2,3-butanediol; 23BE = root exudate collected from 2,3-butanediol-treated root system; 168 = B. subtilis 168; BSIP 1174 = B. subtilis BSIP 1174 (non-producer); BSIP 1171 = B. subtilis BSIP 1174 (overproducer); Pf-5 = Pseudomonas protegens Pf-5; GMI1000 = Ralstonia solanacearum GMI1000; M = MS broth; ME = MS broth amended with root exudate without treatment. (A) Schematic of protocol to extract root exudates after 2,3-butanediol application. (B) Growth kinetics of the three strains in control TSB medium. The figure indicate background expression of three strains 168, 2,3-B(++), and 2,3-B(-). (C) Growth of bacterial strains 168, 2,3-B(++), and 2,3-B(-) after treatment with 2,3-butanedol alone (referred to as 2,3B) or 2,3-butanediol-elicited root exudate (referred to as 2,3BE). (D) Growth kinetics of P. protegens Pf-5, Ralstonia solanacearum GMI1000, and E. coli . P. protegens Pf-5 is a non-pathogenic saprophyte that inhabits soil, water, and plant surface environments. Growth of P. protegens Pf-5 was not inhibited by 2,3-butanediol-elicited exudate. Growth of GMI1000, a soil-borne bacterial wilt pathogen, was inhibited by root exudates. E. coli was included as a bacterial control. Data shown are mean ± SEM of triplicate experiments.

    Article Snippet: Bacterial strains were isolated from plant roots using specific antibiotics in the tryptic soy broth agar growth medium (TSA, BactoTM, BD, Sparks, MD, USA): 50 μg/ml rifampicin for strain 168, 10 μg/ml spectinomycin for 2,3-B(-), and 10 μg/ml spectinomycin plus 5 μg/ml chloramphenicol for 2,3-B(++).

    Techniques: Mutagenesis, Cell Culture, Incubation, Mass Spectrometry, Expressing

    Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into trypticase soy broth in 10-fold serial dilutions of stock starting at 8 ×

    Journal: Immunology

    Article Title: Subversion of interleukin-1-mediated host defence by a nasal carrier strain of Staphylococcus aureus

    doi: 10.1111/j.1365-2567.2008.02952.x

    Figure Lengend Snippet: Carrier but not non-carrier strains of Staphylococcus aureus elaborate a biofilm. Both the nasal carrier strain and the non-carrier strains S. aureus were inoculated into trypticase soy broth in 10-fold serial dilutions of stock starting at 8 ×

    Article Snippet: Staphylococcus aureus (SA) D30 which has been extensively characterized, was originally isolated from the anterior nares of a healthy donor, and served as the carrier strain in the experiments herein., , Staphylococcus aureus 930918-3, (from Ian Holder, Shriners Burn Hospital, Cincinnati, OH) served as the non-carrier strain of S. aureus in these experiments and S. epidermidis was kindly provided by Dr Robert I. Lehrer, University of California (Los Angeles)., Other strains of S. aureus used in the biofilm experiments included S. aureus 502A (an intermittent carrier used in intervention studies in the 1960s), S. aureus D20, S. aureus D98 and S. aureus D85 (designated carrier strains)., , Bacteria were cultivated on trypticase soy agar (TSA, Bacto™; Becton Dickinson and Company, Sparks, MD) and subcultured in tryptic soy broth (TSB; Bacto™) from which stocks were prepared.

    Techniques: