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Difco trypticase soy agar plates
Experimental protocol. TSB , <t>trypticase</t> soy broth
Trypticase Soy Agar Plates, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy agar plates/product/Difco
Average 93 stars, based on 6 article reviews
Price from $9.99 to $1999.99
trypticase soy agar plates - by Bioz Stars, 2020-04
93/100 stars

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1) Product Images from "PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS"

Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS

Journal: The Journal of urology

doi:

Experimental protocol. TSB , trypticase soy broth
Figure Legend Snippet: Experimental protocol. TSB , trypticase soy broth

Techniques Used:

Related Articles

Electron Microscopy:

Article Title: Role of CD14 in Responses to Clinical Isolates of Escherichia coli: Effects of K1 Capsule Expression ▿
Article Snippet: Paragraph title: Electron microscopy of bacterial isolates. ... Briefly, fresh bacterial colonies from Trypticase soy agar plates (Difco, Detroit, MI) were suspended in 0.3 M sucrose, pelleted, fixed by resuspension in 8 ml of 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3) containing 0.15% (wt/vol) RR, and incubated overnight at 4°C.

In Vivo:

Article Title: Antistaphylococcal Activity of WCK 771, a Tricyclic Fluoroquinolone, in Animal Infection Models
Article Snippet: To determine the influence of the NorA efflux pump on the in vivo efficacies of the fluoroquinolones, S. aureus 1199B (NorA+ ) and S. aureus 1199 (NorA− ) systemic infections were established in mice. .. These strains were grown overnight on Trypticase soy agar plates at 37°C and suspended in 5% hog gastric mucin (Difco).

Fluorescence:

Article Title: Invasion of Epithelial Cells and Proteolysis of Cellular Focal Adhesion Components by Distinct Types of Porphyromonas gingivalis Fimbriae
Article Snippet: The organisms were grown anaerobically in GAM broth (Nissui, Tokyo, Japan) or on Trypticase soy agar plates (Difco, BD Diagnostics, Sparks, MD) supplemented with 5% sheep blood (Nihon Biotest, Tokyo, Japan), 5 μg/ml of hemin (Wako Pure Chemical Industries Ltd., Osaka, Japan), and 1 μg/ml of menadione (Sigma-Aldrich, Saint Louis, MO), as described previously ( ). .. The amount of 4-metylcoumaryl-7-amide released was determined at 460 nm with excitation at 380 nm using a fluorescence spectrophotometer (Shimadzu, Kyoto, Japan).

Mutagenesis:

Article Title: Antistaphylococcal Activity of WCK 771, a Tricyclic Fluoroquinolone, in Animal Infection Models
Article Snippet: S. aureus 1199B is a NorA-hyperexpressing strain with a grlA mutation, and S. aureus 1199 is a wild-type strain. .. These strains were grown overnight on Trypticase soy agar plates at 37°C and suspended in 5% hog gastric mucin (Difco).

Article Title: Invasion of Epithelial Cells and Proteolysis of Cellular Focal Adhesion Components by Distinct Types of Porphyromonas gingivalis Fimbriae
Article Snippet: The following P. gingivalis strains were used in this study: ATCC 33277, with type I fimA [ fimA (I)]; HG1691, with fimA (Ib); OMZ314, with fimA (II); 6/26, with fimA (III); HG564, with fimA (IV); HNA99, with fimA (V); KDP150, a fimA mutant of ATCC 33277 [Δ fimA (I)] ( ); and a mutant of OMZ314 with fimA disrupted [Δ fimA (II)] ( ). .. The organisms were grown anaerobically in GAM broth (Nissui, Tokyo, Japan) or on Trypticase soy agar plates (Difco, BD Diagnostics, Sparks, MD) supplemented with 5% sheep blood (Nihon Biotest, Tokyo, Japan), 5 μg/ml of hemin (Wako Pure Chemical Industries Ltd., Osaka, Japan), and 1 μg/ml of menadione (Sigma-Aldrich, Saint Louis, MO), as described previously ( ).

Isolation:

Article Title: Comparison of Subtypes of Listeria monocytogenes Isolates from Naturally Contaminated Watershed Samples with and without a Selective Secondary Enrichment
Article Snippet: Thirty microliters of black Fraser broth cultures were inoculated onto Brilliance Listeria agar plates, and streaked for isolated colonies. .. Up to three colonies that were blue with a zone of clearing on Brilliance plates were picked, deposited onto Trypticase Soy Agar plates (TSA, Difco), and grown overnight at 37°C.

Article Title: Prevalence of shiga toxin producing Escherichia coli, Salmonella enterica, and Listeria monocytogenes at public access watershed sites in a California Central Coast agricultural region
Article Snippet: Paragraph title: L. monocytogenes enrichment, isolation, confirmation, and serotyping ... Up to three colonies that were blue with a zone of clearing on Brilliance plates were picked, deposited onto Trypticase Soy Agar plates (TSA, Difco), and grown overnight at 37°C.

Article Title: Streptococcus mutans isolated from a 4‐year‐old girl diagnosed with infective endocarditis, et al. Streptococcus mutans isolated from a 4‐year‐old girl diagnosed with infective endocarditis
Article Snippet: 2.2 Bacterial strains The bacterial strain isolated from the patient's blood sample, designated here as strain HM, was stored at −70°C until use. .. Both strains were grown anaerobically in brain heart infusion (BHI) broth (Difco Laboratories) and on trypticase soy agar plates (Difco Laboratories) supplemented with defibrinated sheep blood (5% vol/vol).

Article Title: Early Recovery of Salmonella from Food Using a 6-Hour Non-selective Pre-enrichment and Reformulation of Tetrathionate Broth
Article Snippet: Enteritidis strains used for the chicken and egg inoculations were isolated from the respective foods ( Table ). .. Culture stocks, stored at –80°C, were streaked onto Trypticase Soy Agar plates (Difco, Sparks, MD, USA) and incubated overnight at 35 ± 2°C.

Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS
Article Snippet: E. faecalis 210 was initially isolated from the urine of a patient with catheter related urinary tract infection. .. Just before performing the catheter studies bacteria were retrieved from the trypticase soy agar plates and cultured overnight in trypticase soy broth (Difco, Sparks, Maryland).

Mouse Assay:

Article Title: Antistaphylococcal Activity of WCK 771, a Tricyclic Fluoroquinolone, in Animal Infection Models
Article Snippet: To determine the influence of the NorA efflux pump on the in vivo efficacies of the fluoroquinolones, S. aureus 1199B (NorA+ ) and S. aureus 1199 (NorA− ) systemic infections were established in mice. .. These strains were grown overnight on Trypticase soy agar plates at 37°C and suspended in 5% hog gastric mucin (Difco).

Incubation:

Article Title: Comparison of Subtypes of Listeria monocytogenes Isolates from Naturally Contaminated Watershed Samples with and without a Selective Secondary Enrichment
Article Snippet: The remaining 100 μl of bead suspension were inoculated into 5 ml of Fraser broth (Difco) supplemented with Ferric Ammonium Citrate (0.5 g/L), and incubated at 37°C for two days. .. Up to three colonies that were blue with a zone of clearing on Brilliance plates were picked, deposited onto Trypticase Soy Agar plates (TSA, Difco), and grown overnight at 37°C.

Article Title: Early Recovery of Salmonella from Food Using a 6-Hour Non-selective Pre-enrichment and Reformulation of Tetrathionate Broth
Article Snippet: .. Culture stocks, stored at –80°C, were streaked onto Trypticase Soy Agar plates (Difco, Sparks, MD, USA) and incubated overnight at 35 ± 2°C. ..

Article Title: Role of CD14 in Responses to Clinical Isolates of Escherichia coli: Effects of K1 Capsule Expression ▿
Article Snippet: .. Briefly, fresh bacterial colonies from Trypticase soy agar plates (Difco, Detroit, MI) were suspended in 0.3 M sucrose, pelleted, fixed by resuspension in 8 ml of 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3) containing 0.15% (wt/vol) RR, and incubated overnight at 4°C. ..

Article Title: Invasion of Epithelial Cells and Proteolysis of Cellular Focal Adhesion Components by Distinct Types of Porphyromonas gingivalis Fimbriae
Article Snippet: The organisms were grown anaerobically in GAM broth (Nissui, Tokyo, Japan) or on Trypticase soy agar plates (Difco, BD Diagnostics, Sparks, MD) supplemented with 5% sheep blood (Nihon Biotest, Tokyo, Japan), 5 μg/ml of hemin (Wako Pure Chemical Industries Ltd., Osaka, Japan), and 1 μg/ml of menadione (Sigma-Aldrich, Saint Louis, MO), as described previously ( ). .. The cell lysates and culture supernatants were incubated at 37°C for 1 h with the synthetic substrate (100 μM).

Infection:

Article Title: Antistaphylococcal Activity of WCK 771, a Tricyclic Fluoroquinolone, in Animal Infection Models
Article Snippet: Paragraph title: (i) Effect of staphylococcal NorA efflux pump on quinolone efficacy in intraperitoneal infection model. ... These strains were grown overnight on Trypticase soy agar plates at 37°C and suspended in 5% hog gastric mucin (Difco).

Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS
Article Snippet: E. faecalis 210 was initially isolated from the urine of a patient with catheter related urinary tract infection. .. Just before performing the catheter studies bacteria were retrieved from the trypticase soy agar plates and cultured overnight in trypticase soy broth (Difco, Sparks, Maryland).

Sampling:

Article Title: Comparison of Subtypes of Listeria monocytogenes Isolates from Naturally Contaminated Watershed Samples with and without a Selective Secondary Enrichment
Article Snippet: Paragraph title: Sampling and Enrichment ... Up to three colonies that were blue with a zone of clearing on Brilliance plates were picked, deposited onto Trypticase Soy Agar plates (TSA, Difco), and grown overnight at 37°C.

Concentration Assay:

Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS
Article Snippet: Just before performing the catheter studies bacteria were retrieved from the trypticase soy agar plates and cultured overnight in trypticase soy broth (Difco, Sparks, Maryland). .. Bacteria were harvested by centrifugation and re-suspended in sterile saline to a concentration of 108 colony-forming units per ml.

Generated:

Article Title: The Relationship between Quinolone Exposures and Resistance Amplification Is Characterized by an Inverted U: a New Paradigm for Optimizing Pharmacodynamics To Counterselect Resistance ▿
Article Snippet: .. An isogenic ciprofloxacin-resistant MRSA strain (MRSA-CR) was generated by culturing approximately 109 CFU of MRSA-CS onto Trypticase soy agar plates (Difco, Detroit, MI) containing 3× MIC of ciprofloxacin. ..

other:

Article Title: Utility of NCCLS Guidelines for Identifying Extended-Spectrum ?-Lactamases in Non-Escherichia coli and Non-Klebsiella spp. of Enterobacteriaceae
Article Snippet: The bacterial isolates were subcultured from −70°C storage onto trypticase soy agar plates containing 5% defibrinated sheep's blood (Difco; BD BioSciences, Sparks, Md.) and then subcultured once again prior to testing.

Cell Culture:

Article Title: Early Recovery of Salmonella from Food Using a 6-Hour Non-selective Pre-enrichment and Reformulation of Tetrathionate Broth
Article Snippet: Tennessee SALC 76, were cultured from cilantro in our laboratory and the third strain, S . .. Culture stocks, stored at –80°C, were streaked onto Trypticase Soy Agar plates (Difco, Sparks, MD, USA) and incubated overnight at 35 ± 2°C.

Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS
Article Snippet: .. Just before performing the catheter studies bacteria were retrieved from the trypticase soy agar plates and cultured overnight in trypticase soy broth (Difco, Sparks, Maryland). .. Bacteria were harvested by centrifugation and re-suspended in sterile saline to a concentration of 108 colony-forming units per ml.

Spectrophotometry:

Article Title: Invasion of Epithelial Cells and Proteolysis of Cellular Focal Adhesion Components by Distinct Types of Porphyromonas gingivalis Fimbriae
Article Snippet: The organisms were grown anaerobically in GAM broth (Nissui, Tokyo, Japan) or on Trypticase soy agar plates (Difco, BD Diagnostics, Sparks, MD) supplemented with 5% sheep blood (Nihon Biotest, Tokyo, Japan), 5 μg/ml of hemin (Wako Pure Chemical Industries Ltd., Osaka, Japan), and 1 μg/ml of menadione (Sigma-Aldrich, Saint Louis, MO), as described previously ( ). .. The amount of 4-metylcoumaryl-7-amide released was determined at 460 nm with excitation at 380 nm using a fluorescence spectrophotometer (Shimadzu, Kyoto, Japan).

Modification:

Article Title: Role of CD14 in Responses to Clinical Isolates of Escherichia coli: Effects of K1 Capsule Expression ▿
Article Snippet: The presence of a K1 capsule was confirmed by electron microscopy of ruthenium red (RR)-stained E. coli using the modified method of Luft ( ). .. Briefly, fresh bacterial colonies from Trypticase soy agar plates (Difco, Detroit, MI) were suspended in 0.3 M sucrose, pelleted, fixed by resuspension in 8 ml of 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3) containing 0.15% (wt/vol) RR, and incubated overnight at 4°C.

Subculturing Assay:

Article Title: Sensitivity, Specificity, and Predictive Values of Three Salmonella Rapid Detection Kits Using Fresh and Frozen Poultry Environmental Samples versus Those of Standard Plating
Article Snippet: .. The presumptive diagnosis was confirmed by subculturing selected suspect colonies on Trypticase soy agar plates and then testing a single colony with poly-O antisera (Difco Laboratories, Detroit, Mich.). ..

Centrifugation:

Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS
Article Snippet: Just before performing the catheter studies bacteria were retrieved from the trypticase soy agar plates and cultured overnight in trypticase soy broth (Difco, Sparks, Maryland). .. Bacteria were harvested by centrifugation and re-suspended in sterile saline to a concentration of 108 colony-forming units per ml.

Recombinant:

Article Title: Regulation of the Porphyromonas gingivalis fimA (Fimbrillin) Gene
Article Snippet: P. gingivalis strains were grown in Trypticase soy broth (Becton Dickinson) or on 1.5% Trypticase soy broth agar plates, supplemented with yeast extract (1 mg/ml; Difco), hemin (5 μg/ml), and menadione (1 μg/ml), at 37°C in an anaerobic chamber (85% N2 , 10% H2 , 5% CO2 ). .. Escherichia coli DH5α was used as the host strain for recombinant plasmids and grown in L broth with antibiotics—ampicillin (100 μg/ml), kanamycin (50 μg/ml), trimethoprim (200 μg/ml), and tetracycline (10 μg/ml) when necessary.

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    Difco trypticase soy agar plates
    Experimental protocol. TSB , <t>trypticase</t> soy broth
    Trypticase Soy Agar Plates, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar plates/product/Difco
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar plates - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    92
    Difco trypticase soy agar tsa
    Experimental protocol. TSB , <t>trypticase</t> soy broth
    Trypticase Soy Agar Tsa, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar tsa/product/Difco
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar tsa - by Bioz Stars, 2020-04
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      Buy from Supplier

    81
    Difco molten 50°c trypticase soy agar
    Experimental protocol. TSB , <t>trypticase</t> soy broth
    Molten 50°C Trypticase Soy Agar, supplied by Difco, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molten 50°c trypticase soy agar/product/Difco
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    molten 50°c trypticase soy agar - by Bioz Stars, 2020-04
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    Experimental protocol. TSB , trypticase soy broth

    Journal: The Journal of urology

    Article Title: PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS

    doi:

    Figure Lengend Snippet: Experimental protocol. TSB , trypticase soy broth

    Article Snippet: Just before performing the catheter studies bacteria were retrieved from the trypticase soy agar plates and cultured overnight in trypticase soy broth (Difco, Sparks, Maryland).

    Techniques: