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Becton Dickinson trypticase soy agar plate
Trypticase Soy Agar Plate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypticase soy agar plate/product/Becton Dickinson
Average 93 stars, based on 6 article reviews
Price from $9.99 to $1999.99
trypticase soy agar plate - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Electroporation:

Article Title: Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51
Article Snippet: Paragraph title: Electroporation of plasmids into L. monocytogenes Δ2. ... The remaining solution was plated onto a Trypticase soy agar plate (Becton Dickinson) containing 12.5 μg of tetracycline/ml and was incubated at 37°C for 18 h. Resultant tetracycline-resistant colonies were cultured and stored.

Centrifugation:

Article Title: Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Article Snippet: For internalization assays, S. uberis UT888 and UT366, stored at −80°C in 10% skin milk, were thawed in a 37°C water bath, plated onto trypticase soy agar plate supplemented with 5% defibrinated sheep blood (BAP, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and incubated for 16 hours at 37°C. .. Bacterial suspensions were then washed three times by centrifugation (2,500 xg, 15 minutes at 4°C) in phosphate buffer saline (PBS, pH 74), resuspended to original volume in PBS (pH 7.4), and diluted 1 : 100 in Dulbecco's Modified Eagle's Medium (DMEM, Gibco, Grand Island, NY, USA).

Article Title: Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51
Article Snippet: Next, the cell solution was incubated on ice for 10 min, added to 0.7 ml of BHI broth, and incubated at 37°C for 1 h. After centrifugation at 1,200 × g for 15 min at 4°C, 0.6 ml of the solution was removed. .. The remaining solution was plated onto a Trypticase soy agar plate (Becton Dickinson) containing 12.5 μg of tetracycline/ml and was incubated at 37°C for 18 h. Resultant tetracycline-resistant colonies were cultured and stored.

Amplification:

Article Title: Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation, and is dependent on K+ efflux
Article Snippet: Competent cells of TIGR4 were transformed with the resulting amplicon and chloramphenicol-resistant colonies were selected on Fluka Blood Agar Base No. 2 (Sigma Aldrich) supplemented with 5% defibrinated whole sheep blood containing chloramphenicol (4μg/ml). .. Viability was confirmed by plating bacteria in trypticase soy agar plate containing 5% defibrinated sheep blood (BD).

DNA Profiling:

Article Title: Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Article Snippet: Bacterial Species and Culture Conditions The S. uberis strains UT888 and UT366, isolated originally from cows with mastitis, identified using standard bacteriological identification protocols and characterized by PCR-based DNA fingerprinting as described in [ ] were used. .. For internalization assays, S. uberis UT888 and UT366, stored at −80°C in 10% skin milk, were thawed in a 37°C water bath, plated onto trypticase soy agar plate supplemented with 5% defibrinated sheep blood (BAP, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and incubated for 16 hours at 37°C.

In Vitro:

Article Title: Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation, and is dependent on K+ efflux
Article Snippet: Bacteria were grown to mid exponential phase such that there is 108 CFU/ml and diluted in sterile PBS to desired concentrations for corneal infection or in vitro stimulation. .. Viability was confirmed by plating bacteria in trypticase soy agar plate containing 5% defibrinated sheep blood (BD).

Polymerase Chain Reaction:

Article Title: Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Article Snippet: Bacterial Species and Culture Conditions The S. uberis strains UT888 and UT366, isolated originally from cows with mastitis, identified using standard bacteriological identification protocols and characterized by PCR-based DNA fingerprinting as described in [ ] were used. .. For internalization assays, S. uberis UT888 and UT366, stored at −80°C in 10% skin milk, were thawed in a 37°C water bath, plated onto trypticase soy agar plate supplemented with 5% defibrinated sheep blood (BAP, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and incubated for 16 hours at 37°C.

Article Title: Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation, and is dependent on K+ efflux
Article Snippet: PCR and DNA sequencing were carried out to confirm replacement of ply with the cat cassette. .. Viability was confirmed by plating bacteria in trypticase soy agar plate containing 5% defibrinated sheep blood (BD).

Article Title: Genome-wide association analyses of invasive pneumococcal isolates identify a missense bacterial mutation associated with meningitis
Article Snippet: Laboratory strains of S. pneumoniae and PCR primers used in this study are listed in Supplementary Table . .. All strains were maintained in Todd–Hewitt broth supplemented with 0.5% yeast extract (THY) or trypticase soy agar plate with 5% sheep blood (TASII; Becton, Dickinson and Company, MD).

Potency Assay:

Article Title: Modulation of Polymorphonuclear Cell Interleukin-8 Secretion by Human Monoclonal Antibodies to Type 8 Pneumococcal Capsular Polysaccharide
Article Snippet: This model was based on the mouse pneumococcal serum potency assay used to standardize antisera for treatment of pneumococcal pneumonia in the preantibiotic era ( ). .. The number of CFU administered to each mouse was confirmed by plating the inoculum onto a Trypticase agar plate containing 5% sheep's blood (Becton Dickinson, Franklin Lakes, N.J.) and incubating the plates overnight at 37°C, 5% CO2 .

Isolation:

Article Title: Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Article Snippet: Bacterial Species and Culture Conditions The S. uberis strains UT888 and UT366, isolated originally from cows with mastitis, identified using standard bacteriological identification protocols and characterized by PCR-based DNA fingerprinting as described in [ ] were used. .. For internalization assays, S. uberis UT888 and UT366, stored at −80°C in 10% skin milk, were thawed in a 37°C water bath, plated onto trypticase soy agar plate supplemented with 5% defibrinated sheep blood (BAP, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and incubated for 16 hours at 37°C.

Article Title: Antimicrobial Resistance in Salmonella enterica Serovar Heidelberg Isolates from Retail Meats, Including Poultry, from 2002 to 2006 ▿
Article Snippet: .. Positive Tecra or Vidas samples were streaked onto a xylose-lysine-deoxycholate agar plate for isolation and incubated at 35°C for 24 h. When Salmonella -like growth was observed, one well-isolated colony was streaked onto a trypticase soy agar plate supplemented with 5% defribrinated sheep blood (BBL, Becton, Dickinson, and Company, Sparks, MD) for isolation. ..

Cell Culture:

Article Title: Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51
Article Snippet: .. The remaining solution was plated onto a Trypticase soy agar plate (Becton Dickinson) containing 12.5 μg of tetracycline/ml and was incubated at 37°C for 18 h. Resultant tetracycline-resistant colonies were cultured and stored. .. These were named Δ2/p3L118R, Δ2/p3L118R-Ag85A, Δ2/p3L118R-Ag85B, and Δ2/p3L118R-MPB51 and harbored the recombinant plasmids p3L118R, p3L118R-Ag85A, p3L118R-Ag85B, and p3L118R-MPB51, respectively.

Mouse Assay:

Article Title: Modulation of Polymorphonuclear Cell Interleukin-8 Secretion by Human Monoclonal Antibodies to Type 8 Pneumococcal Capsular Polysaccharide
Article Snippet: The i.t. infection was performed as described previously ( ) and as follows: groups of 10 C4−/− mice were anesthetized i.p. with 6.5 mg of sodium pentobarbital (Abbott Laboratories, North Chicago, Ill.)/kg of body weight, a tracheal incision was made, and each mouse was given 20 CFU of pneumococci with either PBS, 1 μg of control IgM or D11, or 10 μg of control IgA or NAD. .. The number of CFU administered to each mouse was confirmed by plating the inoculum onto a Trypticase agar plate containing 5% sheep's blood (Becton Dickinson, Franklin Lakes, N.J.) and incubating the plates overnight at 37°C, 5% CO2 .

Concentration Assay:

Article Title: Genome-wide association analyses of invasive pneumococcal isolates identify a missense bacterial mutation associated with meningitis
Article Snippet: All strains were maintained in Todd–Hewitt broth supplemented with 0.5% yeast extract (THY) or trypticase soy agar plate with 5% sheep blood (TASII; Becton, Dickinson and Company, MD). .. Competence Stimulating Peptide-1 (CSP-1, final concentration 500 ng ml−1 ) was used in all transformations.

Article Title: Modulation of Polymorphonuclear Cell Interleukin-8 Secretion by Human Monoclonal Antibodies to Type 8 Pneumococcal Capsular Polysaccharide
Article Snippet: The number of CFU administered to each mouse was confirmed by plating the inoculum onto a Trypticase agar plate containing 5% sheep's blood (Becton Dickinson, Franklin Lakes, N.J.) and incubating the plates overnight at 37°C, 5% CO2 . .. The concentration of D11 used corresponded to the amount of MAb that was previously shown to protect 86% of mice against death from systemic disease ( ).

Incubation:

Article Title: Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Article Snippet: .. For internalization assays, S. uberis UT888 and UT366, stored at −80°C in 10% skin milk, were thawed in a 37°C water bath, plated onto trypticase soy agar plate supplemented with 5% defibrinated sheep blood (BAP, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and incubated for 16 hours at 37°C. .. After incubation, bacterial lawns were harvested, resuspended in 20 mL Todd Hewitt broth (THB, Becton Dickinson Co., Sparks, MD), and incubated with orbital shaking (150 rpm) for 2 hours at 37°C (C24 Incubator Shaking, New Brunswick Scientific, Eden, NJ, USA).

Article Title: Antimicrobial Resistance in Salmonella enterica Serovar Heidelberg Isolates from Retail Meats, Including Poultry, from 2002 to 2006 ▿
Article Snippet: .. Positive Tecra or Vidas samples were streaked onto a xylose-lysine-deoxycholate agar plate for isolation and incubated at 35°C for 24 h. When Salmonella -like growth was observed, one well-isolated colony was streaked onto a trypticase soy agar plate supplemented with 5% defribrinated sheep blood (BBL, Becton, Dickinson, and Company, Sparks, MD) for isolation. ..

Article Title: Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51
Article Snippet: .. The remaining solution was plated onto a Trypticase soy agar plate (Becton Dickinson) containing 12.5 μg of tetracycline/ml and was incubated at 37°C for 18 h. Resultant tetracycline-resistant colonies were cultured and stored. .. These were named Δ2/p3L118R, Δ2/p3L118R-Ag85A, Δ2/p3L118R-Ag85B, and Δ2/p3L118R-MPB51 and harbored the recombinant plasmids p3L118R, p3L118R-Ag85A, p3L118R-Ag85B, and p3L118R-MPB51, respectively.

Article Title: In vitro susceptibility of Propionibacterium acnes to simulated intrawound vancomycin concentrations
Article Snippet: A 10-µL volume of the original sample and each serial dilution was then dropped onto a trypticase soy agar plate with 5% sheep blood (Becton Dickinson, Sparks, MD, USA). .. Experimental samples and positive controls from the same time point were placed on the same blood agar plate and then placed back in the anaerobic chamber for incubation × 3 days at 38°C.

Article Title: Characterization of Erysipelothrix Species Isolates from Clinically Affected Pigs, Environmental Samples, and Vaccine Strains from Six Recent Swine Erysipelas Outbreaks in the United States ▿
Article Snippet: .. Samples were cut into 2- by 3-cm sections, added to 2 ml of 0.85% physiologic saline solution, and homogenized using a stomacher (Seward, Bohemia, NY), and 300 μl of the resulting tissue homogenate was added to 3 ml of Erysipelothrix species-selective broth and incubated at 35°C for 24 to 48 h. At 24 h and again at 48 h, a 100-μl subculture from the Erysipelothrix species-selective broth was put onto a Trypticase soy agar plate containing 5% sheep blood, a colistin-nalidixic acid (Becton Dickinson) agar plate containing 5% sheep blood, and an Erysipelothrix -selective plate as described previously ( ). .. Colonies were subcultured on sheep blood agar plates, incubated for 24 h, and then biochemically confirmed using standard laboratory methods ( , ).

Article Title: The Efficacy of Pneumococcal Capsular Polysaccharide-specific Antibodies to Serotype 3 Streptococcus pneumoniae requires Macrophages
Article Snippet: .. To confirm the amount of ST3 administered, diluted pneumococci were plated onto a Trypticase agar plate containing 5% sheep's blood (Becton Dickinson, Franklin Lakes, NJ), incubated overnight at 5% CO2 at 37°C and counted the following day. .. A7 [IgM(κ)] is a human PPS3-specific MAb, derived from XenoMouse™ mice, that protects mice from death after intraperitoneal (i.p.) challenge with ST3 [ ; ].

other:

Article Title: Low prevalence of rmpA and high tendency of rmpA mutation correspond to low virulence of extended spectrum β-lactamase-producing klebsiella pneumoniae isolates
Article Snippet: The hypermucoviscosity phenotype was defined positive as a viscous string of > 5 mm of the colony on trypticase soy agar plate with 5% sheep blood (BD Diagnostics, MD, USA).

Article Title: The Anti-Inflammatory and Proresolving Mediator Resolvin E1 Protects Mice from Bacterial Pneumonia and Acute Lung Injury
Article Snippet: Trypticase soy agar plate with 5% sheep blood was obtained from BD Biosciences (Franklin Lakes, NJ).

Infection:

Article Title: Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation, and is dependent on K+ efflux
Article Snippet: Bacteria were grown to mid exponential phase such that there is 108 CFU/ml and diluted in sterile PBS to desired concentrations for corneal infection or in vitro stimulation. .. Viability was confirmed by plating bacteria in trypticase soy agar plate containing 5% defibrinated sheep blood (BD).

Article Title: Modulation of Polymorphonuclear Cell Interleukin-8 Secretion by Human Monoclonal Antibodies to Type 8 Pneumococcal Capsular Polysaccharide
Article Snippet: Paragraph title: Infection and survival studies. ... The number of CFU administered to each mouse was confirmed by plating the inoculum onto a Trypticase agar plate containing 5% sheep's blood (Becton Dickinson, Franklin Lakes, N.J.) and incubating the plates overnight at 37°C, 5% CO2 .

Expressing:

Article Title: Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51
Article Snippet: One hundred microliters of the cell suspension and 1 μg of one of the expression plasmids were then transferred to an electroporation cuvette and subjected to electroshock with a Gene-Pulser electroporation apparatus (Bio-Rad Laboratories, Hercules, Calif.). .. The remaining solution was plated onto a Trypticase soy agar plate (Becton Dickinson) containing 12.5 μg of tetracycline/ml and was incubated at 37°C for 18 h. Resultant tetracycline-resistant colonies were cultured and stored.

Serial Dilution:

Article Title: In vitro susceptibility of Propionibacterium acnes to simulated intrawound vancomycin concentrations
Article Snippet: .. A 10-µL volume of the original sample and each serial dilution was then dropped onto a trypticase soy agar plate with 5% sheep blood (Becton Dickinson, Sparks, MD, USA). .. Experimental samples and positive controls from the same time point were placed on the same blood agar plate and then placed back in the anaerobic chamber for incubation × 3 days at 38°C.

Modification:

Article Title: Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells
Article Snippet: For internalization assays, S. uberis UT888 and UT366, stored at −80°C in 10% skin milk, were thawed in a 37°C water bath, plated onto trypticase soy agar plate supplemented with 5% defibrinated sheep blood (BAP, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and incubated for 16 hours at 37°C. .. Bacterial suspensions were then washed three times by centrifugation (2,500 xg, 15 minutes at 4°C) in phosphate buffer saline (PBS, pH 74), resuspended to original volume in PBS (pH 7.4), and diluted 1 : 100 in Dulbecco's Modified Eagle's Medium (DMEM, Gibco, Grand Island, NY, USA).

Sample Prep:

Article Title: Characterization of Erysipelothrix Species Isolates from Clinically Affected Pigs, Environmental Samples, and Vaccine Strains from Six Recent Swine Erysipelas Outbreaks in the United States ▿
Article Snippet: Paragraph title: Sample preparation. (i) Tissue specimens. ... Samples were cut into 2- by 3-cm sections, added to 2 ml of 0.85% physiologic saline solution, and homogenized using a stomacher (Seward, Bohemia, NY), and 300 μl of the resulting tissue homogenate was added to 3 ml of Erysipelothrix species-selective broth and incubated at 35°C for 24 to 48 h. At 24 h and again at 48 h, a 100-μl subculture from the Erysipelothrix species-selective broth was put onto a Trypticase soy agar plate containing 5% sheep blood, a colistin-nalidixic acid (Becton Dickinson) agar plate containing 5% sheep blood, and an Erysipelothrix -selective plate as described previously ( ).

Transformation Assay:

Article Title: Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation, and is dependent on K+ efflux
Article Snippet: Competent cells of TIGR4 were transformed with the resulting amplicon and chloramphenicol-resistant colonies were selected on Fluka Blood Agar Base No. 2 (Sigma Aldrich) supplemented with 5% defibrinated whole sheep blood containing chloramphenicol (4μg/ml). .. Viability was confirmed by plating bacteria in trypticase soy agar plate containing 5% defibrinated sheep blood (BD).

Recombinant:

Article Title: Induction of Protective Cellular Immunity against Mycobacterium tuberculosis by Recombinant Attenuated Self-Destructing Listeria monocytogenes Strains Harboring Eukaryotic Expression Plasmids for Antigen 85 Complex and MPB/MPT51
Article Snippet: The remaining solution was plated onto a Trypticase soy agar plate (Becton Dickinson) containing 12.5 μg of tetracycline/ml and was incubated at 37°C for 18 h. Resultant tetracycline-resistant colonies were cultured and stored. .. These were named Δ2/p3L118R, Δ2/p3L118R-Ag85A, Δ2/p3L118R-Ag85B, and Δ2/p3L118R-MPB51 and harbored the recombinant plasmids p3L118R, p3L118R-Ag85A, p3L118R-Ag85B, and p3L118R-MPB51, respectively.

DNA Sequencing:

Article Title: Neutrophil IL-1β processing induced by pneumolysin is mediated by the NLRP3/ASC inflammasome and caspase-1 activation, and is dependent on K+ efflux
Article Snippet: PCR and DNA sequencing were carried out to confirm replacement of ply with the cat cassette. .. Viability was confirmed by plating bacteria in trypticase soy agar plate containing 5% defibrinated sheep blood (BD).

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    Becton Dickinson trypticase soy agar
    Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or <t>Trypticase</t> soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .
    Trypticase Soy Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypticase soy agar/product/Becton Dickinson
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    trypticase soy agar - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

    Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

    doi: 10.1093/jbcr/iry043

    Figure Lengend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Article Snippet: The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD).

    Techniques: Infection, Serial Dilution, Isolation

    Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Journal: Journal of Burn Care & Research: Official Publication of the American Burn Association

    Article Title: Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model

    doi: 10.1093/jbcr/iry043

    Figure Lengend Snippet: Bacterial counts recovered from infected partial-thickness burn tissue over 11 days post-burn. P. aeruginosa (A) and total CFU counts (B) recovered as determined by serial dilution and plating on either P. aeruginosa isolation agar or Trypticase soy agar containing 5% sheep's blood, respectively. C. Total P. aeruginosa (live and dead) counts as determined by P. aeruginosa -specific primers. D. Total CFU counts as determined by general gram-negative primers. E. Percentage of total gram-negative bacterial load in the burn tissue that is P. aeruginosa . As time post-infection increased, P. aeruginosa accounted for a greater number of total bacterial cells isolated from wound tissue. P. aeruginosa eventually dominated as the primary wound pathogen. Burns without P. aeruginosa inoculation also became colonized with bacterial cells to the same level as the inoculated groups, but had greater diversity of gram-negative and positive cells. PA , P. aeruginosa .

    Article Snippet: The samples were serial diluted with PBS and plated on Trypticase soy agar containing 5% sheep’s blood (Becton, Dickinson and Co.) as well as P. aeruginosa isolation agar (Hardy Diagnostics, Santa Maria, CA) using a WASP 2 Spiral Plater (Microbiology International, Frederick, MD).

    Techniques: Infection, Serial Dilution, Isolation

    Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns

    doi: 10.1128/AAC.46.6.1837-1844.2002

    Figure Lengend Snippet: Microbicidal kinetics. P. aeruginosa strain 2 was exposed to the indicated concentrations of novispirin G10 in a medium composed of 100 mM NaCl, 10 mM sodium phosphate buffer (pH 7.4), and 0.3 mg of Trypticase soy broth powder/ml. Aliquots were removed after 5 and 10 min, diluted, spread on Trypticase soy agar plates, and incubated overnight to allow colony development.

    Article Snippet: After 4 h, treated areas of the infected wound tissues were harvested aseptically, weighed, homogenized, serially diluted, and plated in triplicate on Trypticase soy agar with 5% sheep blood and Pseudomonas isolation agar (both from Becton Dickinson).

    Techniques: Incubation