tryptic soy agar  (Becton Dickinson)

Journal: Journal of Immunology (Baltimore, Md. : 1950)

Article Title: Ecto-5′-nucleotidase (CD73) decreases mortality and organ injury in sepsis

doi: 10.4049/jimmunol.1003379

Figure Lengend Snippet: Loss of CD73 decreases survival, and increases bacterial load and inflammation in polymicrobial sepsis (A) The number of surviving CD73 WT and KO mice were counted daily for 7 days after inducing sepsis by cecal ligation and puncture (CLP). (B) Blood and peritoneal lavage fluid obtained from CD73 WT and KO mice 16 h post CLP were cultured on soy-trypticase agar plates for 24 h and then the bacterial colonies were counted (n = 13 mice per group). Serum and peritoneal lavage fluid concentration of TNF-α (C), IL-1β (D), IL12p40 (E), IL-6 (F), IL-10 (G), MCP-1 (H), MIP-1α (I) and MIP-2 (J) were determined in samples collected 16 h post CLP using ELISA. All results (mean ± SEM) shown are representative of three separate experiments. * p

Article Snippet: Fifty μl of each dilution was aseptically plated and cultured on trypticase blood agar plates (BD Biosciences) at 37°C.

Techniques: Mouse Assay, Ligation, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Infection and Immunity

Article Title: Bordetella pertussis Autoregulates Pertussis Toxin Production through the Metabolism of Cysteine

doi: 10.1128/IAI.69.11.6823-6830.2001

Figure Lengend Snippet: Decoupling of Ptx production with growth of B. pertussis strain CS-87 culture in SS medium. The bacteria were cultured in a New Brunswick 20-liter BioFlo 4500 fermentor running in batch mode with a working volume of 12 liters at 36.5°C. The OD of the cultures was measured at 650 nm. Culture purity was verified by Gram staining and plating on BGA and Trypticase soy agar. The OD of the cultures and the average [Ptx]/OD are compared. The average Ptx concentrations for two samples from each time point were used to calculate the [Ptx]/OD values. The coefficient of variance for each Ptx sample was

Article Snippet: Culture purity was verified by gram staining and plating on BGA and Trypticase soy agar (Becton-Dickinson).

Techniques: Cell Culture, Staining

Journal: Journal of Clinical Microbiology

Article Title: Cutaneous Infection Caused by Gordonia amicalis after a Traumatic Injury

doi: 10.1128/JCM.06681-11

Figure Lengend Snippet: Orange, opaque, dry, and nonhemolytic colonies grew on Trypticase soy agar supplemented with 5% sheep blood after incubation for 72 h.

Article Snippet: Cultures on Trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Sparks, MD) grew small numbers of slightly orange and dry colonies and Gram-positive coryneform bacilli with rudimentary branching and weakly acid-fast bacilli after incubation for 4 days.

Techniques: Incubation

Journal: Microbiology Resource Announcements

Article Title: Complete Genome Sequences of Two Nosocomiicoccus ampullae Strains and a Growth-Adapted Mutant

doi: 10.1128/MRA.00747-21

Figure Lengend Snippet: (A) Growth of Nosocomiicoccus ampullae strain 19-00310 on TSA at different temperatures with and without the addition of 0.5 M NaCl. When the strain was cultivated at 37°C or 42°C, a diffused pigment is visible in the agar. (B) Genome alignment of strains 19-00310 and DSM 19163 is shown at the bottom (made with Easyfig v2.2.5 [ 12 ]). The insertion of a putative prophage in strain 19-00310 is shown at the top. G+C content is shaded in blue (GC/GCAT

Article Snippet: The isolate grew at 30°C to 42°C on tryptic soy agar (TSA) (BD) plates but showed the best growth at 37°C with 0.5 M NaCl added.

Techniques: