trypsinedta  (Millipore)


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  • 99
    Name:
    Trypsin EDTA solution
    Description:

    Catalog Number:
    t4174
    Price:
    None
    Applications:
    The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsin-EDTA solution is used for the following applications:. Used as a supplement in cell culture for their maintenance. In harvesting cells grown to confluence. to detach lentivirus-transduced macrophages
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    Structured Review

    Millipore trypsinedta

    https://www.bioz.com/result/trypsinedta/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsinedta - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Cell Uptake and Validation of Novel PECs for Biomedical Applications
    Article Snippet: .. The suppliers of the chemicals were as follows: fetal bovine serum (FBS, Sigma, USA, CAS:N/A), penicillin-streptomycin solution (Sigma, USA, CAS:010M0651), L-glutamine 200 mM (Sigma, USA, CAS:56-85-9), DMEM medium (Sigma, USA, CAS:N/A), sodium pyruvate (Sigma, USA, CAS:113-24-6), Trypsin-EDTA solution (Sigma, USA, CAS:N/A), thiazolyl blue tetrazolium bromide (MTT, Sigma, USA, CAS:MKBB9557), phosphate buffered saline (PBS, Sigma, USA, CAS:049K8204), Trypan Blue solution (Sigma, USA, CAS:72-57-1), Fluoroshield with DAPI (Sigma, USA, CAS:070M1418). ..

    Transferring:

    Article Title: Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
    Article Snippet: .. Cell culture plasticware T75 and/or T25 flasks (Corning, catalog numbers: 430641U for T75 and 3056 for T25) Plates low cell binding, 96 wells, round bottom (Thermo Fisher Scientific, Nunc™, catalog number: 145399) Centrifuge tubes (15 ml; 50 ml, Corning, catalog numbers: 430790; 430828) Cryovials (STARLAB, catalog number: E3110-6122) Plastic stripettes (5 ml; 10 ml; 25 ml, Corning, Costar® , catalog numbers: 4487; 4488; 4489) Pipette tips (TipOne, STARLAB, catalog numbers: S1111-3700; S1111-1706; S1111-6701) Non-adherent microfuge tubes (Eppendorf, catalog number: 0030108116) Reagents DMEM low glucose (Sigma-Aldrich, catalog number: D6046) Fetal bovine serum (FBS, Thermo Fisher Scientific, Gibco™, catalog number: 10500064) Gelatin, Analar (BDH, catalog number: 440454B) L-Glutamine, 200 mM (Sigma-Aldrich, catalog number: G7513) MesenPRO RS™ Medium (Thermo Fisher Scientific, Gibco™, catalog number: 12746012) PBS, without calcium and magnesium (Sigma-Aldrich, catalog number: D8537) Penicillin-streptomycin (P/S), 10,000 units penicillin and 10 mg streptomycin per ml (Sigma-Aldrich, catalog number: P0781) Trypsin/EDTA 10x (Sigma-Aldrich, catalog number: T4174) Triton X-100 (Sigma-Aldrich, catalog number: T8787) Trypan blue solution (Sigma-Aldrich, catalog number: T8154, 0.4% [w/v] solution) Paraformaldehyde (Sigma-Aldrich, catalog number: P6148) Methanol (Fisher Scientific, CAS: 67-56-1) Fish skin gelatin (Sigma-Aldrich, catalog number:G7041) Antibodies: Mouse anti-fibrillin (used at 1/200, Merck, catalog number: MAB1919) Rabbit anti-fibronectin (used at 1/200, Sigma-Aldrich, catalog number: F3648) Donkey anti-mouse 680 nm (used at 1/400, LI-COR, catalog number: 926-68072) Donkey anti-rabbit 488 nM (used at 1/500, Thermo Fisher Scientific, Invitrogen™, catalog number: ) DAPI (used at 1/100,000,Thermo Fisher Scientific, Invitrogen™, catalog number: D1306) Gelatin solution (see ) MesenPRO RS™ Medium (see ) DMEM low glucose (see ) Trypsin (see ) .. Water bath, 37 °C (Grant JB Nova) CO2 Incubator (Eppendorf, New Brunswick™, model: Galaxy® 170 S) Glass hemocytometer (Brand) Laminar flow hood (ENVAIR, model: Envair Eco Safe Basic Plus) Inverted microscope (Olympus, model: CKX31) Moticam 2300 camera coupled to Motic Images Plus 2.0 ML software (Motic, model: Moticam 2300) Cell culture centrifuge (Sigma Laborzentrifugen, model: 3-16KL) Aspirator (dry vacuum pump/compressor, Welch Vacuum - Gardner Denver, model: 2511) Autoclave (Prestige Medical, model: Classic 2100 Extended, catalog number: 210004UK)

    Cell Culture:

    Article Title: A comparative study on the biological activity of essential oil and total hydro-alcoholic extract of Satureja hortensis L.
    Article Snippet: .. The reagents used for cell culture: Dulbecco's modified Eagle's medium (DMEM) was provided from ATTC and trypsin/EDTA solution, phosphate-buffered saline (PBS), penicillin/streptomycin mixture, fetal calf serum (FCS) and Trypan blue solution were supplied by Sigma-Aldrich; Merck KGaA. ..

    Article Title: Chitosan/polyethylene glycol fumarate blend films for wound dressing application: in vitro biocompatibility and biodegradability assays
    Article Snippet: .. Cultured mouse fibroblasts (L929, the cell bank of National Institute of Genetic Engineering and Biotechnology, Iran) were harvested with a 0.25% Trypsin–EDTA solution (Sigma) in a phosphate-buffered saline (PBS; pH 7.4) and re-suspended in the culture medium composed of RPMI-1640 with l -glutamine (Sigma, USA), supplemented with 10% fetal bovine serum (FBS, Gibco, Germany), 1% Pen-Strep (100 IU/mL penicillin and 10 mg/mL streptomycin, Sigma-Aldrich, USA). .. A cell suspension was prepared with a concentration of approximately 1 × 106 cell/mL and seeded onto 12-well plates in contact with the sterilized films.

    Article Title: Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
    Article Snippet: .. Cell culture plasticware T75 and/or T25 flasks (Corning, catalog numbers: 430641U for T75 and 3056 for T25) Plates low cell binding, 96 wells, round bottom (Thermo Fisher Scientific, Nunc™, catalog number: 145399) Centrifuge tubes (15 ml; 50 ml, Corning, catalog numbers: 430790; 430828) Cryovials (STARLAB, catalog number: E3110-6122) Plastic stripettes (5 ml; 10 ml; 25 ml, Corning, Costar® , catalog numbers: 4487; 4488; 4489) Pipette tips (TipOne, STARLAB, catalog numbers: S1111-3700; S1111-1706; S1111-6701) Non-adherent microfuge tubes (Eppendorf, catalog number: 0030108116) Reagents DMEM low glucose (Sigma-Aldrich, catalog number: D6046) Fetal bovine serum (FBS, Thermo Fisher Scientific, Gibco™, catalog number: 10500064) Gelatin, Analar (BDH, catalog number: 440454B) L-Glutamine, 200 mM (Sigma-Aldrich, catalog number: G7513) MesenPRO RS™ Medium (Thermo Fisher Scientific, Gibco™, catalog number: 12746012) PBS, without calcium and magnesium (Sigma-Aldrich, catalog number: D8537) Penicillin-streptomycin (P/S), 10,000 units penicillin and 10 mg streptomycin per ml (Sigma-Aldrich, catalog number: P0781) Trypsin/EDTA 10x (Sigma-Aldrich, catalog number: T4174) Triton X-100 (Sigma-Aldrich, catalog number: T8787) Trypan blue solution (Sigma-Aldrich, catalog number: T8154, 0.4% [w/v] solution) Paraformaldehyde (Sigma-Aldrich, catalog number: P6148) Methanol (Fisher Scientific, CAS: 67-56-1) Fish skin gelatin (Sigma-Aldrich, catalog number:G7041) Antibodies: Mouse anti-fibrillin (used at 1/200, Merck, catalog number: MAB1919) Rabbit anti-fibronectin (used at 1/200, Sigma-Aldrich, catalog number: F3648) Donkey anti-mouse 680 nm (used at 1/400, LI-COR, catalog number: 926-68072) Donkey anti-rabbit 488 nM (used at 1/500, Thermo Fisher Scientific, Invitrogen™, catalog number: ) DAPI (used at 1/100,000,Thermo Fisher Scientific, Invitrogen™, catalog number: D1306) Gelatin solution (see ) MesenPRO RS™ Medium (see ) DMEM low glucose (see ) Trypsin (see ) .. Water bath, 37 °C (Grant JB Nova) CO2 Incubator (Eppendorf, New Brunswick™, model: Galaxy® 170 S) Glass hemocytometer (Brand) Laminar flow hood (ENVAIR, model: Envair Eco Safe Basic Plus) Inverted microscope (Olympus, model: CKX31) Moticam 2300 camera coupled to Motic Images Plus 2.0 ML software (Motic, model: Moticam 2300) Cell culture centrifuge (Sigma Laborzentrifugen, model: 3-16KL) Aspirator (dry vacuum pump/compressor, Welch Vacuum - Gardner Denver, model: 2511) Autoclave (Prestige Medical, model: Classic 2100 Extended, catalog number: 210004UK)

    other:

    Article Title: Culture media-based selection of endothelial cells, pericytes, and perivascular-resident macrophage-like melanocytes from the young mouse vestibular system
    Article Snippet: The cells (first passage, ~7 - 10 d) were detached from the culture dish with a solution of Trypsin-EDTA (catalog # T4049, Sigma) and counted with a hemacytometer (Model no. 1483, Hausser Scientific, Horsham, PA, USA).

    Modification:

    Article Title: A comparative study on the biological activity of essential oil and total hydro-alcoholic extract of Satureja hortensis L.
    Article Snippet: .. The reagents used for cell culture: Dulbecco's modified Eagle's medium (DMEM) was provided from ATTC and trypsin/EDTA solution, phosphate-buffered saline (PBS), penicillin/streptomycin mixture, fetal calf serum (FCS) and Trypan blue solution were supplied by Sigma-Aldrich; Merck KGaA. ..

    Binding Assay:

    Article Title: Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
    Article Snippet: .. Cell culture plasticware T75 and/or T25 flasks (Corning, catalog numbers: 430641U for T75 and 3056 for T25) Plates low cell binding, 96 wells, round bottom (Thermo Fisher Scientific, Nunc™, catalog number: 145399) Centrifuge tubes (15 ml; 50 ml, Corning, catalog numbers: 430790; 430828) Cryovials (STARLAB, catalog number: E3110-6122) Plastic stripettes (5 ml; 10 ml; 25 ml, Corning, Costar® , catalog numbers: 4487; 4488; 4489) Pipette tips (TipOne, STARLAB, catalog numbers: S1111-3700; S1111-1706; S1111-6701) Non-adherent microfuge tubes (Eppendorf, catalog number: 0030108116) Reagents DMEM low glucose (Sigma-Aldrich, catalog number: D6046) Fetal bovine serum (FBS, Thermo Fisher Scientific, Gibco™, catalog number: 10500064) Gelatin, Analar (BDH, catalog number: 440454B) L-Glutamine, 200 mM (Sigma-Aldrich, catalog number: G7513) MesenPRO RS™ Medium (Thermo Fisher Scientific, Gibco™, catalog number: 12746012) PBS, without calcium and magnesium (Sigma-Aldrich, catalog number: D8537) Penicillin-streptomycin (P/S), 10,000 units penicillin and 10 mg streptomycin per ml (Sigma-Aldrich, catalog number: P0781) Trypsin/EDTA 10x (Sigma-Aldrich, catalog number: T4174) Triton X-100 (Sigma-Aldrich, catalog number: T8787) Trypan blue solution (Sigma-Aldrich, catalog number: T8154, 0.4% [w/v] solution) Paraformaldehyde (Sigma-Aldrich, catalog number: P6148) Methanol (Fisher Scientific, CAS: 67-56-1) Fish skin gelatin (Sigma-Aldrich, catalog number:G7041) Antibodies: Mouse anti-fibrillin (used at 1/200, Merck, catalog number: MAB1919) Rabbit anti-fibronectin (used at 1/200, Sigma-Aldrich, catalog number: F3648) Donkey anti-mouse 680 nm (used at 1/400, LI-COR, catalog number: 926-68072) Donkey anti-rabbit 488 nM (used at 1/500, Thermo Fisher Scientific, Invitrogen™, catalog number: ) DAPI (used at 1/100,000,Thermo Fisher Scientific, Invitrogen™, catalog number: D1306) Gelatin solution (see ) MesenPRO RS™ Medium (see ) DMEM low glucose (see ) Trypsin (see ) .. Water bath, 37 °C (Grant JB Nova) CO2 Incubator (Eppendorf, New Brunswick™, model: Galaxy® 170 S) Glass hemocytometer (Brand) Laminar flow hood (ENVAIR, model: Envair Eco Safe Basic Plus) Inverted microscope (Olympus, model: CKX31) Moticam 2300 camera coupled to Motic Images Plus 2.0 ML software (Motic, model: Moticam 2300) Cell culture centrifuge (Sigma Laborzentrifugen, model: 3-16KL) Aspirator (dry vacuum pump/compressor, Welch Vacuum - Gardner Denver, model: 2511) Autoclave (Prestige Medical, model: Classic 2100 Extended, catalog number: 210004UK)

    Fluorescence In Situ Hybridization:

    Article Title: Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
    Article Snippet: .. Cell culture plasticware T75 and/or T25 flasks (Corning, catalog numbers: 430641U for T75 and 3056 for T25) Plates low cell binding, 96 wells, round bottom (Thermo Fisher Scientific, Nunc™, catalog number: 145399) Centrifuge tubes (15 ml; 50 ml, Corning, catalog numbers: 430790; 430828) Cryovials (STARLAB, catalog number: E3110-6122) Plastic stripettes (5 ml; 10 ml; 25 ml, Corning, Costar® , catalog numbers: 4487; 4488; 4489) Pipette tips (TipOne, STARLAB, catalog numbers: S1111-3700; S1111-1706; S1111-6701) Non-adherent microfuge tubes (Eppendorf, catalog number: 0030108116) Reagents DMEM low glucose (Sigma-Aldrich, catalog number: D6046) Fetal bovine serum (FBS, Thermo Fisher Scientific, Gibco™, catalog number: 10500064) Gelatin, Analar (BDH, catalog number: 440454B) L-Glutamine, 200 mM (Sigma-Aldrich, catalog number: G7513) MesenPRO RS™ Medium (Thermo Fisher Scientific, Gibco™, catalog number: 12746012) PBS, without calcium and magnesium (Sigma-Aldrich, catalog number: D8537) Penicillin-streptomycin (P/S), 10,000 units penicillin and 10 mg streptomycin per ml (Sigma-Aldrich, catalog number: P0781) Trypsin/EDTA 10x (Sigma-Aldrich, catalog number: T4174) Triton X-100 (Sigma-Aldrich, catalog number: T8787) Trypan blue solution (Sigma-Aldrich, catalog number: T8154, 0.4% [w/v] solution) Paraformaldehyde (Sigma-Aldrich, catalog number: P6148) Methanol (Fisher Scientific, CAS: 67-56-1) Fish skin gelatin (Sigma-Aldrich, catalog number:G7041) Antibodies: Mouse anti-fibrillin (used at 1/200, Merck, catalog number: MAB1919) Rabbit anti-fibronectin (used at 1/200, Sigma-Aldrich, catalog number: F3648) Donkey anti-mouse 680 nm (used at 1/400, LI-COR, catalog number: 926-68072) Donkey anti-rabbit 488 nM (used at 1/500, Thermo Fisher Scientific, Invitrogen™, catalog number: ) DAPI (used at 1/100,000,Thermo Fisher Scientific, Invitrogen™, catalog number: D1306) Gelatin solution (see ) MesenPRO RS™ Medium (see ) DMEM low glucose (see ) Trypsin (see ) .. Water bath, 37 °C (Grant JB Nova) CO2 Incubator (Eppendorf, New Brunswick™, model: Galaxy® 170 S) Glass hemocytometer (Brand) Laminar flow hood (ENVAIR, model: Envair Eco Safe Basic Plus) Inverted microscope (Olympus, model: CKX31) Moticam 2300 camera coupled to Motic Images Plus 2.0 ML software (Motic, model: Moticam 2300) Cell culture centrifuge (Sigma Laborzentrifugen, model: 3-16KL) Aspirator (dry vacuum pump/compressor, Welch Vacuum - Gardner Denver, model: 2511) Autoclave (Prestige Medical, model: Classic 2100 Extended, catalog number: 210004UK)

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