Journal: Biochemistry

Article Title: Identification of Kazal Inhibitor Scaffolds with Identical Canonical Binding Loops and Their Effects on Binding Properties

doi: 10.1021/acs.biochem.2c00573

Figure Lengend Snippet: SPINK1 sequence and structure conservation. (A) Phylogenetic analysis of trypsin-targeting SPINK1 homologues. Duplicates indicate different isoforms within the same species. SPINK1 variants featuring arginine as the P1 residue are highlighted in red. (B) Structure of the canonical binding loop (PDB ID: 7QE8 ) colored according to conservation using the multiple sequence alignment from (A). Conservation scores were determined using the ConSurf server. (C) Multiple sequence alignment of selected SPINK1 variants and consensus sequences of (A) subclustered according to P1 residues. (D) Structure of binding pockets in trypsin. The S1 pocket is highlighted in red, the oxyanion hole in blue, and L104 interacting with the P2 residue in gray.

Article Snippet: Human trypsin isoforms were expressed in E. coli BL21 (DE3) (New England Biolabs) overnight at 30 °C.

Techniques: Sequencing, Binding Assay