trypsin versene  (Lonza)


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  • 95
    Name:
    Versene
    Description:
    Versene EDTA 0 02 0 2 g L Ethylenediaminetetraacetic acid 0 53mM in DBPS without calcium or magnesium 100 mL
    Catalog Number:
    BE17-711E
    Price:
    None
    Category:
    Culture Media and Reagents
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    Structured Review

    Lonza trypsin versene
    Versene EDTA 0 02 0 2 g L Ethylenediaminetetraacetic acid 0 53mM in DBPS without calcium or magnesium 100 mL
    https://www.bioz.com/result/trypsin versene/product/Lonza
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin versene - by Bioz Stars, 2021-06
    95/100 stars

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    Related Articles

    Electroporation:

    Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
    Article Snippet: .. For experiments performed in Ficoll, cells were plated in a T25 flask immediately after electroporation and media was exchanged for fresh RAW media after 1 h. Prior to imaging, cells were incubated with EDTA (17-711E; Lonza) for 10 min to detach them from the flask, spun for 90 s at 1173 rpm, and resuspended in 100 μL of media containing 23% Ficoll400 (F8016; Sigma Aldrich). .. A 4×5 mm glass cylinder (7030304; Bioptechs) was fixed to a glass bottom dish using silicon grease and 25 μL of cell suspension in 23% Ficoll was pipetted into the cylinder.

    Imaging:

    Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
    Article Snippet: .. For experiments performed in Ficoll, cells were plated in a T25 flask immediately after electroporation and media was exchanged for fresh RAW media after 1 h. Prior to imaging, cells were incubated with EDTA (17-711E; Lonza) for 10 min to detach them from the flask, spun for 90 s at 1173 rpm, and resuspended in 100 μL of media containing 23% Ficoll400 (F8016; Sigma Aldrich). .. A 4×5 mm glass cylinder (7030304; Bioptechs) was fixed to a glass bottom dish using silicon grease and 25 μL of cell suspension in 23% Ficoll was pipetted into the cylinder.

    Incubation:

    Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
    Article Snippet: .. For experiments performed in Ficoll, cells were plated in a T25 flask immediately after electroporation and media was exchanged for fresh RAW media after 1 h. Prior to imaging, cells were incubated with EDTA (17-711E; Lonza) for 10 min to detach them from the flask, spun for 90 s at 1173 rpm, and resuspended in 100 μL of media containing 23% Ficoll400 (F8016; Sigma Aldrich). .. A 4×5 mm glass cylinder (7030304; Bioptechs) was fixed to a glass bottom dish using silicon grease and 25 μL of cell suspension in 23% Ficoll was pipetted into the cylinder.

    Article Title: Hypoxic marker CA IX and adhesion mediator β-catenin are downregulated by lymphocytic choriomeningitis virus persistent infection
    Article Snippet: .. For flow cytometry the cells were washed 2 times for 10 min with PBS pH 7.2 and to all cells 1 ml of versene solution (Lonza, Verviers, Belgium) per well was added and incubated for 5 min at 4°C. ..

    other:

    Article Title: Critical role of the JNK-p53-GADD45? apoptotic cascade in mediating oxidative cytotoxicity in hippocampal neurons
    Article Snippet: The antibiotics solution containing 10 000 U·mL−1 penicillin and 10 mg·mL−1 streptomycin was obtained from Invitrogen (Carlsbad, CA), and trypsin-versene mixture (containing 0.25% trypsin and 0.02% EDTA) from Lonza Walkersville (Walkersville, MD, USA).

    Article Title: Effect of cAMP derivates on assembly and maintenance of tight junctions in human umbilical vein endothelial cells
    Article Snippet: To generate a calcium-free environment the cells were cultivated for 2 h in EGM medium in the presence of EDTA (3 μM) to chelate the free calcium or in PBS without any calcium but supplemented with all growth factors contained in the Lonza single quots.

    Flow Cytometry:

    Article Title: Hypoxic marker CA IX and adhesion mediator β-catenin are downregulated by lymphocytic choriomeningitis virus persistent infection
    Article Snippet: .. For flow cytometry the cells were washed 2 times for 10 min with PBS pH 7.2 and to all cells 1 ml of versene solution (Lonza, Verviers, Belgium) per well was added and incubated for 5 min at 4°C. ..

    Cytometry:

    Article Title: Hypoxic marker CA IX and adhesion mediator β-catenin are downregulated by lymphocytic choriomeningitis virus persistent infection
    Article Snippet: .. For flow cytometry the cells were washed 2 times for 10 min with PBS pH 7.2 and to all cells 1 ml of versene solution (Lonza, Verviers, Belgium) per well was added and incubated for 5 min at 4°C. ..

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  • 97
    Lonza g0 by trypsination
    Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in <t>G0</t> by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).
    G0 By Trypsination, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g0 by trypsination/product/Lonza
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g0 by trypsination - by Bioz Stars, 2021-06
    97/100 stars
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    94
    Lonza trypsin edta solution
    Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in <t>G0</t> by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).
    Trypsin Edta Solution, supplied by Lonza, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta solution/product/Lonza
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trypsin edta solution - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in G0 by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).

    Journal: PLoS ONE

    Article Title: The Human Homolog of Escherichia coli Endonuclease V Is a Nucleolar Protein with Affinity for Branched DNA Structures

    doi: 10.1371/journal.pone.0047466

    Figure Lengend Snippet: Upregulation of ENDOV transcription during quiescence. ( A ) Human embryonic fibroblasts were arrested in G0 by serum starvation at confluence and released by replating 1∶4 in culture medium with serum. ENDOV transcript levels (exons 2 to 3) were measured during cell cycle progression after G0 release at indicated time points by qRT-PCR. G0 cells were used as the reference for calculations. The average of 3 parallels (same RNA) was calculated and standard deviation is shown. Cell cycle distribution was monitored using propidium iodide staining followed by flow cytometry after release from the block. The percentage of cells in each cell cycle is presented in the table. The experiment was repeated twice with similar results. ( B ) Nothern blot analysis of ENDOV mRNA. mRNA was isolated from human fibroblasts that were unsynchronised (u), G0 arrested (G0) and allowed to proliferate for 24 hours (t 24 ), separated by electrophoresis and transferred to a nylon membrane. Hybridisation signals with probes spanning exons 4–8, exon 10, exon 3 of ENDOV and for β -ACTIN are shown. M is the RNA size standard as indicated (in kilobases).

    Article Snippet: Cell cycle synchronization and analysis by flow cytometrySynchronization of the cells in G0 phase was achieved by culturing cells as a confluent layer for 72 h followed by serum starvation (0.2% serum) for 72 h. The cells were released from G0 by trypsination (Trypsin-EDTA 200 mg/l, Lonza) for 4 min at 37°C and cultivated in standard growth medium at 25% confluence.

    Techniques: Quantitative RT-PCR, Standard Deviation, Staining, Flow Cytometry, Blocking Assay, Isolation, Electrophoresis, Hybridization