truseq small rna library kit  (Illumina Inc)

 
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    Name:
    TruSeq Small RNA Library Prep Kit Set A
    Description:
    TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA MicroRNAs miRNAs generated by Dicer processing are efficiently targeted by the included modified adapters These kits enable multiplexed sequencing with the introduction of 48 unique indexes allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing Indexes are added in a universal amplification reaction greatly reducing ligation bias and ensuring accurate measurement of miRNA expression Workflow improvements enable streamlined sample preparation allowing economical studies covering all small RNA transcripts in any species Compatible applications include finding novel miRNAs characterizing variation such as isomers with single base resolution and analyzing differential expression without prior assumptions TruSeq Small RNA Sample Datasets The Human Brain Reference RNA HBRR and Universal Human Reference RNA UHRR samples were prepared using the TruSeq Small RNA Library Prep Kit These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration The total yield was 0 91 Gb with 97 of bases at or above Q30 Browse the Data In BaseSpace Sequence Hub View Run primary analysis and metrics View Project secondary analysis with BaseSpace Apps
    Catalog Number:
    rs-200-0012
    Price:
    None
    Category:
    Library Preparation Kits
    Buy from Supplier


    Structured Review

    Illumina Inc truseq small rna library kit
    TruSeq Small RNA Library Prep Kit Set A
    TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA MicroRNAs miRNAs generated by Dicer processing are efficiently targeted by the included modified adapters These kits enable multiplexed sequencing with the introduction of 48 unique indexes allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing Indexes are added in a universal amplification reaction greatly reducing ligation bias and ensuring accurate measurement of miRNA expression Workflow improvements enable streamlined sample preparation allowing economical studies covering all small RNA transcripts in any species Compatible applications include finding novel miRNAs characterizing variation such as isomers with single base resolution and analyzing differential expression without prior assumptions TruSeq Small RNA Sample Datasets The Human Brain Reference RNA HBRR and Universal Human Reference RNA UHRR samples were prepared using the TruSeq Small RNA Library Prep Kit These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration The total yield was 0 91 Gb with 97 of bases at or above Q30 Browse the Data In BaseSpace Sequence Hub View Run primary analysis and metrics View Project secondary analysis with BaseSpace Apps
    https://www.bioz.com/result/truseq small rna library kit/product/Illumina Inc
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    truseq small rna library kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs
    Article Snippet: .. cDNA amplification and Illumina sequencing cDNA amplification was performed using the TruSeq Small RNA Sample Preparation Kit (Illumina) according to the manufacturer's protocol. .. Briefly, 6 μl of ligated RNA and 1 μl of RTP primer were incubated at 70°C for 2 min and then placed on ice.

    RNA Sequencing Assay:

    Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification
    Article Snippet: .. Most of the kits tested, including Illumina TruSeq Small RNA Library Prep Kit, Lexogen Small RNA-Seq Library Prep Kit, New England Biolabs NEBNext Small RNA Library Prep Set, PerkinElmer (formerly Bioo Scientific) NextFlex Small RNA-Seq Kit v.3, Qiagen QIAseq miRNA Library Kit, and Trilink CleanTag Small RNA Library Prep Kit all use variants of this methodology. .. The Takara SMARTer smRNA Kit and the Diagenode CATS Small RNA-Seq Kit both begin by polyadenylation of the 3′ nucleotide of the smRNA followed by reverse transcription and template switching.

    Article Title: Small RNA Sequencing of Sporadic Amyotrophic Lateral Sclerosis Cerebrospinal Fluid Reveals Differentially Expressed miRNAs Related to Neural and Glial Activity
    Article Snippet: .. Small RNA-sequencing RNA was prepared for small RNA-sequencing using the Illumina TruSeq Small RNA library preparation kit following a modified protocol as described in Burgos et al. ( ). ..

    Ligation:

    Article Title: nextPARS: parallel probing of RNA structures in Illumina
    Article Snippet: .. In brief, our adapted protocol couples initial phosphatase and kinase treatments after the RNase probing step, to allow ligation of the corresponding 5′ and 3′ adaptors of the Illumina TruSeq Small RNA Sample Preparation Kit. .. Then a reverse transcription of the RNA fragments and a PCR amplification are performed to obtain sequencing-ready libraries.

    Article Title: YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs
    Article Snippet: .. For conventional ligation, 2 pmol of synthetic tRNA was subjected to 5΄- and 3΄- adapter ligation using the TruSeq Small RNA Sample Preparation Kit (Illumina). .. The respective ligation products (1/100 volume, 20 fmol of tRNA) were subjected to reverse transcription using SuperScript III Reverse Transcriptase (Life Technologies) in a total reaction volume of 20 μl; 0.4 μl of each reaction mixture was further subjected to Real-time PCR using SsoFast™ EvaGreen® Supermix (BioRad) and a StepOne PlusTM Real-time PCR machine (Applied Biosystems).

    Modification:

    Article Title: Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation
    Article Snippet: .. Samples prepared with modified adapters were compared to the TruSeq small RNA Library Preparation Kit (Illumina). .. The TruSeq kit recommends a minimum of 1000 ng RNA input in combination with a gel purification step after library preparation; however, we also tested this kit at lower RNA inputs.

    Article Title: Small RNA Sequencing of Sporadic Amyotrophic Lateral Sclerosis Cerebrospinal Fluid Reveals Differentially Expressed miRNAs Related to Neural and Glial Activity
    Article Snippet: .. Small RNA-sequencing RNA was prepared for small RNA-sequencing using the Illumina TruSeq Small RNA library preparation kit following a modified protocol as described in Burgos et al. ( ). ..

    Sequencing:

    Article Title: Identification of extracellular miRNA in archived serum samples by next-generation sequencing from RNA extracted using multiple methods
    Article Snippet: .. The small RNA library preparation was carried out using Illumina TruSeq small RNA kit and sequencing was carried out using Illumina platform. ..

    Article Title: YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs
    Article Snippet: .. cDNA amplification and Illumina sequencing cDNA amplification was performed using the TruSeq Small RNA Sample Preparation Kit (Illumina) according to the manufacturer's protocol. .. Briefly, 6 μl of ligated RNA and 1 μl of RTP primer were incubated at 70°C for 2 min and then placed on ice.

    Sample Prep:

    Article Title: nextPARS: parallel probing of RNA structures in Illumina
    Article Snippet: .. In brief, our adapted protocol couples initial phosphatase and kinase treatments after the RNase probing step, to allow ligation of the corresponding 5′ and 3′ adaptors of the Illumina TruSeq Small RNA Sample Preparation Kit. .. Then a reverse transcription of the RNA fragments and a PCR amplification are performed to obtain sequencing-ready libraries.

    Article Title: YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs
    Article Snippet: .. cDNA amplification and Illumina sequencing cDNA amplification was performed using the TruSeq Small RNA Sample Preparation Kit (Illumina) according to the manufacturer's protocol. .. Briefly, 6 μl of ligated RNA and 1 μl of RTP primer were incubated at 70°C for 2 min and then placed on ice.

    Article Title: YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs
    Article Snippet: .. For conventional ligation, 2 pmol of synthetic tRNA was subjected to 5΄- and 3΄- adapter ligation using the TruSeq Small RNA Sample Preparation Kit (Illumina). .. The respective ligation products (1/100 volume, 20 fmol of tRNA) were subjected to reverse transcription using SuperScript III Reverse Transcriptase (Life Technologies) in a total reaction volume of 20 μl; 0.4 μl of each reaction mixture was further subjected to Real-time PCR using SsoFast™ EvaGreen® Supermix (BioRad) and a StepOne PlusTM Real-time PCR machine (Applied Biosystems).

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  • 94
    Illumina Inc truseq small rna sample kit
    a Average percentage of reads mapped to the human transcriptome to <t>RNA</t> biotypes for Illumina <t>TruSeq</t> and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex
    Truseq Small Rna Sample Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna sample kit/product/Illumina Inc
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    truseq small rna sample kit - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    99
    Illumina Inc truseq small rna kit
    Small <t>RNA</t> analysis performed by using the Agilent 2100 Bioanalyzer and and the sequencing library preparation using Illumina <t>TruSeq</t> Small RNA kit: a miRNA/sRNA ratio: Ratio of miRNA molecules expressed as a portion of all small RNA molecules (%) detected with the Agilent 2100 Bioanalyzer. b Electronic gel image of small RNA library obtained using Illumina TruSeq Small RNA kit for the unpooled samples where carrier was added to the TRIzol extraction procedure
    Truseq Small Rna Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq small rna kit/product/Illumina Inc
    Average 99 stars, based on 395 article reviews
    Price from $9.99 to $1999.99
    truseq small rna kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Journal: BMC Genomics

    Article Title: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

    doi: 10.1186/s12864-018-4726-6

    Figure Lengend Snippet: a Average percentage of reads mapped to the human transcriptome to RNA biotypes for Illumina TruSeq and BiooScientific NEXTFlex for the plasma samples. The percentages presented here are averaged over the 6 different volumes for each kit respectively. b PCA plot of the plasma samples show clustering of the samples by kit-type and not by input volume. c Number of miRNAs detected at three expression thresholds: > 1 read per million mapped to the genome (RPM), > 10 RPM and > 100 RPM for all the plasma samples for Illumina TruSeq and BiooScientific NEXTFlex

    Article Snippet: Illumina TruSeq small RNA library preparation Small RNA libraries were generated using Illumina TruSeq Small RNA Sample kit (RS-200-0048; Illumina).

    Techniques: Expressing

    Schematic of study design. Tissue RNA from brain, liver and placenta were sequenced at two sites at two input amounts (1 μg and 10 ng) using three different RNA sequencing kits (Illumina TruSeq, NEB Next and BiooScientific NEXTFlex). RNA from plasma samples at 5 different input volumes (200 μL – 5 mL) were sequenced at Site 1 using only TruSeq and BiooScientific. The green arrow depicts the flow of one of the tissue samples – brain using NEB Next and the red arrows, the plasma samples. The RNASeq results from the tissue samples were then validated using three different platforms (qPCR, EdgeSeq performed by Site1 and Fireplex performed by Site2). For a full list of samples sequenced, please refer to Additional file 1 : Table S1

    Journal: BMC Genomics

    Article Title: Evaluation of commercially available small RNASeq library preparation kits using low input RNA

    doi: 10.1186/s12864-018-4726-6

    Figure Lengend Snippet: Schematic of study design. Tissue RNA from brain, liver and placenta were sequenced at two sites at two input amounts (1 μg and 10 ng) using three different RNA sequencing kits (Illumina TruSeq, NEB Next and BiooScientific NEXTFlex). RNA from plasma samples at 5 different input volumes (200 μL – 5 mL) were sequenced at Site 1 using only TruSeq and BiooScientific. The green arrow depicts the flow of one of the tissue samples – brain using NEB Next and the red arrows, the plasma samples. The RNASeq results from the tissue samples were then validated using three different platforms (qPCR, EdgeSeq performed by Site1 and Fireplex performed by Site2). For a full list of samples sequenced, please refer to Additional file 1 : Table S1

    Article Snippet: Illumina TruSeq small RNA library preparation Small RNA libraries were generated using Illumina TruSeq Small RNA Sample kit (RS-200-0048; Illumina).

    Techniques: RNA Sequencing Assay, Flow Cytometry, Real-time Polymerase Chain Reaction

    Small RNA analysis performed by using the Agilent 2100 Bioanalyzer and and the sequencing library preparation using Illumina TruSeq Small RNA kit: a miRNA/sRNA ratio: Ratio of miRNA molecules expressed as a portion of all small RNA molecules (%) detected with the Agilent 2100 Bioanalyzer. b Electronic gel image of small RNA library obtained using Illumina TruSeq Small RNA kit for the unpooled samples where carrier was added to the TRIzol extraction procedure

    Journal: Molecular Biology Reports

    Article Title: Identification of extracellular miRNA in archived serum samples by next-generation sequencing from RNA extracted using multiple methods

    doi: 10.1007/s11033-016-4043-6

    Figure Lengend Snippet: Small RNA analysis performed by using the Agilent 2100 Bioanalyzer and and the sequencing library preparation using Illumina TruSeq Small RNA kit: a miRNA/sRNA ratio: Ratio of miRNA molecules expressed as a portion of all small RNA molecules (%) detected with the Agilent 2100 Bioanalyzer. b Electronic gel image of small RNA library obtained using Illumina TruSeq Small RNA kit for the unpooled samples where carrier was added to the TRIzol extraction procedure

    Article Snippet: The small RNA library preparation was carried out using Illumina TruSeq small RNA kit and sequencing was carried out using Illumina platform.

    Techniques: Sequencing

    Adapter-tRNA ligation efficiencies with a conventional method and YAMAT-seq. ( A ) Schematic representation of the adapter ligation reactions used in the conventional and YAMAT-seq methods. In the conventional method, tRNA was subjected to 3΄-adapter (3΄-AD) and 5΄-adapter (5΄-AD) ligations catalyzed by truncated Rnl2 and Rnl1, respectively, using the Illumina TruSeq Small RNA Sample Preparation Kit. In contrast, in the YAMAT-seq method, a Y-shaped adapter (Y-AD) is ligated to a tRNA by Rnl2. ( B ) Ligation products of synthetic human cyto tRNA AspGUC and 5΄-AD or 3΄-AD were quantified by real-time qRT-PCR. The amounts of ligation products were determined based on standard curves, and ligation efficiencies were calculated as percentages of ligated tRNA versus input tRNA (set as 100%). Each data set represents the average of three independent experiments with bars showing the SD. ( C ) Ligation products of the indicated native cyto tRNAs in BT-474 total RNA and 5΄-AD or 3΄-AD were quantified by real-time qRT-PCR. Each data set represents the average Ct values of three independent experiments with bars showing the SD. ( D ) Image of 8% native polyacrylamide gel electrophoresis (PAGE) of amplified cDNAs resulting from BT-474 total RNA sequencing by conventional and YAMAT-seq procedures. The total adapter lengths of the conventional and YAMAT-seq procedures are 118 and 140 bp, respectively. The YAMAT-seq-derived cDNAs in the designated Bands 1–3 regions were gel-purified and subjected to cloning; the identified sequences are shown in Supplementary Table S1 . The cDNAs in regions designated with gray lines are expected to contain tRNA fraction.

    Journal: Nucleic Acids Research

    Article Title: YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs

    doi: 10.1093/nar/gkx005

    Figure Lengend Snippet: Adapter-tRNA ligation efficiencies with a conventional method and YAMAT-seq. ( A ) Schematic representation of the adapter ligation reactions used in the conventional and YAMAT-seq methods. In the conventional method, tRNA was subjected to 3΄-adapter (3΄-AD) and 5΄-adapter (5΄-AD) ligations catalyzed by truncated Rnl2 and Rnl1, respectively, using the Illumina TruSeq Small RNA Sample Preparation Kit. In contrast, in the YAMAT-seq method, a Y-shaped adapter (Y-AD) is ligated to a tRNA by Rnl2. ( B ) Ligation products of synthetic human cyto tRNA AspGUC and 5΄-AD or 3΄-AD were quantified by real-time qRT-PCR. The amounts of ligation products were determined based on standard curves, and ligation efficiencies were calculated as percentages of ligated tRNA versus input tRNA (set as 100%). Each data set represents the average of three independent experiments with bars showing the SD. ( C ) Ligation products of the indicated native cyto tRNAs in BT-474 total RNA and 5΄-AD or 3΄-AD were quantified by real-time qRT-PCR. Each data set represents the average Ct values of three independent experiments with bars showing the SD. ( D ) Image of 8% native polyacrylamide gel electrophoresis (PAGE) of amplified cDNAs resulting from BT-474 total RNA sequencing by conventional and YAMAT-seq procedures. The total adapter lengths of the conventional and YAMAT-seq procedures are 118 and 140 bp, respectively. The YAMAT-seq-derived cDNAs in the designated Bands 1–3 regions were gel-purified and subjected to cloning; the identified sequences are shown in Supplementary Table S1 . The cDNAs in regions designated with gray lines are expected to contain tRNA fraction.

    Article Snippet: cDNA amplification and Illumina sequencing cDNA amplification was performed using the TruSeq Small RNA Sample Preparation Kit (Illumina) according to the manufacturer's protocol.

    Techniques: Ligation, Sample Prep, Quantitative RT-PCR, Polyacrylamide Gel Electrophoresis, Amplification, RNA Sequencing Assay, Derivative Assay, Purification, Clone Assay