truseq rna library prep kit v2  (Illumina Inc)

 
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    Name:
    TruSeq RNA Library Preparation Kit v2
    Description:
    Generate mRNA focused sequencing libraries from total RNA with enhanced multiplex capability and a simple workflow with master mixed reagents Multiplex Capabilities Kits feature 24 unique indexes delivering enhanced multiplex performance for processing large numbers of samples The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process This allows the samples to be pooled and then individually identified during downstream analysis Minimal Hands On Steps Master mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean up as compared to previous methods minimizing hands on time This results in economical high throughput RNA sequencing studies achieved with a user friendly workflow Automation Options Find an up to date list of automation vendors with robotic systems compatible with this kit
    Catalog Number:
    rs-122-2001
    Price:
    None
    Category:
    Library Preparation Kits
    Buy from Supplier


    Structured Review

    Illumina Inc truseq rna library prep kit v2
    TruSeq RNA Library Preparation Kit v2
    Generate mRNA focused sequencing libraries from total RNA with enhanced multiplex capability and a simple workflow with master mixed reagents Multiplex Capabilities Kits feature 24 unique indexes delivering enhanced multiplex performance for processing large numbers of samples The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process This allows the samples to be pooled and then individually identified during downstream analysis Minimal Hands On Steps Master mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean up as compared to previous methods minimizing hands on time This results in economical high throughput RNA sequencing studies achieved with a user friendly workflow Automation Options Find an up to date list of automation vendors with robotic systems compatible with this kit
    https://www.bioz.com/result/truseq rna library prep kit v2/product/Illumina Inc
    Average 90 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    truseq rna library prep kit v2 - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
    Article Snippet: RNA libraries were prepared using Illumina TruSeq RNA Sample Prep LT version 2 (RS-122-2001/2002). .. Illumina Reagents (Part #15012995) were used for PCR amplification.

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA. .. The cDNA libraries were amplified and sequenced using the cBot and HiSeq2000 from Illumina (SR; 1 × 50 bp; 6 GB ca.

    Article Title: H3K4 Methylation-Dependent Memory of Somatic Cell Identity Inhibits Reprogramming and Development of Nuclear Transfer Embryos
    Article Snippet: .. Per sample, 500 ng RNA was used to generate a cDNA sequencing libraries using a Illumina TrueSeq kit (RS-122-2001), according to the manufacturer’s protocol using 12 PCR amplification cycles. .. cDNA synthesis and RTqPCR analysis cDNA synthesis was performed from the isolated RNAs using oligo dT(15) primers.

    Article Title: Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye
    Article Snippet: .. RNA-Seq library preps were made using the Illumina TruSeq RNA sample Prep Kit v2 (Cat #RS-122-2002), using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50. .. For each RNA-seq sample, sequence quality was assessed with FastQC ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc ) and sequencing adapters were removed with Trimmomatic [ ].

    Construct:

    Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
    Article Snippet: .. RNA-seq and ChIP-seq libraries were constructed using a TruSeq RNA Library Preparation Kit (Illumina; RS-122-2002) and the Ovation Ultralow DR Multiplex System (NuGEN; 0330), respectively. ..

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: RNA-seq libraries were constructed as previously described in Sultan et al., 2012 [ ] with minor modifications. .. Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev.

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001). .. Transcriptome libraries were constructed by polyA purification.

    Article Title: Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera
    Article Snippet: .. cDNA library construction for de novo Sequencing Tissues from 10 seedlings (3 month old whole seedling) were pooled together and RNA was isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA) and subsequently used to construct library with TruSeq RNA Sample preparation kit V2 (Illumina, Cat RS-122-2002) following manufactures protocol. .. 1 μg of total RNA by Qubit was enriched for Poly A using RNA Purification Beads.

    Quantitation Assay:

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor™ dsDNA System (Promega, Mannheim, Germany).

    Expressing:

    Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
    Article Snippet: RNA-seq and ChIP-seq libraries were constructed using a TruSeq RNA Library Preparation Kit (Illumina; RS-122-2002) and the Ovation Ultralow DR Multiplex System (NuGEN; 0330), respectively. .. For RNA-seq, TopHat (2.0.8b) and Cufflinks (2.1.1) were used for differential expression analysis ( ).

    Article Title: Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
    Article Snippet: RNA-seq libraries were generated using TruSeq RNA library prep kit (Illumina, RS-122-2001/2) per the manufacturer’s instructions. .. Sequencing reads were aligned to the human genome (hg19) using RUM, and differential expression was analyzed using edgeR.

    Modification:

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev. .. Further steps: purification, end-repair, A-tailing and adapter ligation were performed as described in the previously mentioned TruSeq kit protocol with one modification: the first purification eluate was not decanted from the magnetic beads and subsequent steps were performed with the beads in solution.

    Article Title: Transposable elements modulate human RNA abundance and splicing via specific RNA-protein interactions
    Article Snippet: We extracted RNA using the buffer RWT/3X isopropanol modification detailed in ‘Appendix B: Optional On-Column DNAse Digestion…’ of the Qiagen miRNeasy® Mini Handbook. .. To remove ribosomal RNA, we fed ≥70 ng of input and fRIP RNA into the Ribo-Zero™ Magnetic Gold Kit (Epicentre; Madison, WI, USA; catalog number MRZG12324) followed by a cleanup using Agencourt RNAClean XP beads (Beckman Coulter; Brea, CA, USA; catalog number A63987) and elution with 19.5 μl of Elute, Prime, Fragment mix from the TruSeq RNA Sample Preparation Kit (Illumina; San Diego, CA, USA; catalog number RS-122-2001).

    Ligation:

    Article Title: The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
    Article Snippet: RNA libraries were prepared using Illumina TruSeq RNA Sample Prep LT version 2 (RS-122-2001/2002). .. Complementary DNA libraries were created from the enriched RNA using Invitrogen Superscript II® per the manufacturers protocol. cDNA was fragmented, end repaired, and adenylated followed by a ligation of Illumina adaptor indices.

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev. .. Further steps: purification, end-repair, A-tailing and adapter ligation were performed as described in the previously mentioned TruSeq kit protocol with one modification: the first purification eluate was not decanted from the magnetic beads and subsequent steps were performed with the beads in solution.

    Generated:

    Article Title: Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
    Article Snippet: .. RNA-seq libraries were generated using TruSeq RNA library prep kit (Illumina, RS-122-2001/2) per the manufacturer’s instructions. .. The libraries were sequenced as 100bp single end using a HiSeq 2500 sequencer (Illumina).

    Sequencing:

    Article Title: Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
    Article Snippet: .. For ES and iPS cells, we prepared samples for sequencing using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001). .. The samples were quantitated with Life Technologies Qubit fluorometer (Q32857).

    Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
    Article Snippet: Paragraph title: Library Construction, Sequencing, and Data Analysis ... RNA-seq and ChIP-seq libraries were constructed using a TruSeq RNA Library Preparation Kit (Illumina; RS-122-2002) and the Ovation Ultralow DR Multiplex System (NuGEN; 0330), respectively.

    Article Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
    Article Snippet: Total RNA extraction from purified islet is described above. mRNA from whole islets was used to generate libraries using Illumina TruSeq RNA Sample Prep v2 (RS-122-2001). .. All sequence data were performed in biological triplicates, each containing islets from 1-2 animals, at 2 x 50 bp length with high quality metrics ( > 20 Phred score) and nucleotide distribution.

    Article Title: The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
    Article Snippet: Paragraph title: 2.5. Transcriptome Illumina Paired-End Library Construction and Sequencing ... RNA libraries were prepared using Illumina TruSeq RNA Sample Prep LT version 2 (RS-122-2001/2002).

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001). .. In brief, 1 μg of total RNA from each sample was used to construct a cDNA library, followed by sequencing on the Illumina HiSeq 2500 system with single end 50 bp reads and ~30 millions of reads per sample.

    Article Title: Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
    Article Snippet: RNA-seq libraries were generated using TruSeq RNA library prep kit (Illumina, RS-122-2001/2) per the manufacturer’s instructions. .. Sequencing reads were aligned to the human genome (hg19) using RUM, and differential expression was analyzed using edgeR.

    Article Title: Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera
    Article Snippet: .. cDNA library construction for de novo Sequencing Tissues from 10 seedlings (3 month old whole seedling) were pooled together and RNA was isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA) and subsequently used to construct library with TruSeq RNA Sample preparation kit V2 (Illumina, Cat RS-122-2002) following manufactures protocol. .. 1 μg of total RNA by Qubit was enriched for Poly A using RNA Purification Beads.

    Article Title: Transposable elements modulate human RNA abundance and splicing via specific RNA-protein interactions
    Article Snippet: To remove ribosomal RNA, we fed ≥70 ng of input and fRIP RNA into the Ribo-Zero™ Magnetic Gold Kit (Epicentre; Madison, WI, USA; catalog number MRZG12324) followed by a cleanup using Agencourt RNAClean XP beads (Beckman Coulter; Brea, CA, USA; catalog number A63987) and elution with 19.5 μl of Elute, Prime, Fragment mix from the TruSeq RNA Sample Preparation Kit (Illumina; San Diego, CA, USA; catalog number RS-122-2001). .. We pooled the resulting cDNA libraries and subjected them to paired-end sequencing on an Illumina HiSeq 2500 at a depth of 31 base pairs per read.

    Article Title: H3K4 Methylation-Dependent Memory of Somatic Cell Identity Inhibits Reprogramming and Development of Nuclear Transfer Embryos
    Article Snippet: .. Per sample, 500 ng RNA was used to generate a cDNA sequencing libraries using a Illumina TrueSeq kit (RS-122-2001), according to the manufacturer’s protocol using 12 PCR amplification cycles. .. cDNA synthesis and RTqPCR analysis cDNA synthesis was performed from the isolated RNAs using oligo dT(15) primers.

    Article Title: Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye
    Article Snippet: Library preparation and sequencing was carried out by the NYU Genome Technology Center. .. RNA-Seq library preps were made using the Illumina TruSeq RNA sample Prep Kit v2 (Cat #RS-122-2002), using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50.

    Article Title: Identification of a unique gene expression signature in mercury and 2,3,7,8-tetrachlorodibenzo-p-dioxin co-exposed cells
    Article Snippet: RNA-Seq libraries were prepared using an Illumina TruSeq RNA Sample Preparation Kit (RS-122-2002) according to the manufacturer’s protocol. .. Sequencing was performed as described earlier , using an Illumina HiSeq 2500 to obtain 50-nucleotide single-end reads.

    ChIP-sequencing:

    Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
    Article Snippet: .. RNA-seq and ChIP-seq libraries were constructed using a TruSeq RNA Library Preparation Kit (Illumina; RS-122-2002) and the Ovation Ultralow DR Multiplex System (NuGEN; 0330), respectively. ..

    Article Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
    Article Snippet: Paragraph title: Bulk RNA-seq and ChIP-seq ... Total RNA extraction from purified islet is described above. mRNA from whole islets was used to generate libraries using Illumina TruSeq RNA Sample Prep v2 (RS-122-2001).

    Nucleic Acid Electrophoresis:

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: RNA was digested with RNAse-free DNAse (Qiagen, Hilden, Germany) and checked for integrity by capillary gel electrophoresis (Bioanalyzer, Agilent Technologies, Inc., Böblingen, Germany). .. Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA.

    RNA Sequencing Assay:

    Article Title: Recent polyploidization events in three Saccharum founding species
    Article Snippet: Paragraph title: RNA‐Seq analysis ... A pair‐end library for LA Purple was made using Illumina® TruSeq™ RNA Sample Preparation Kit (RS‐122‐2001(2), Illumina).

    Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
    Article Snippet: .. RNA-seq and ChIP-seq libraries were constructed using a TruSeq RNA Library Preparation Kit (Illumina; RS-122-2002) and the Ovation Ultralow DR Multiplex System (NuGEN; 0330), respectively. ..

    Article Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
    Article Snippet: Paragraph title: Bulk RNA-seq and ChIP-seq ... Total RNA extraction from purified islet is described above. mRNA from whole islets was used to generate libraries using Illumina TruSeq RNA Sample Prep v2 (RS-122-2001).

    Article Title: Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels
    Article Snippet: Paragraph title: 2.9 RNA sequencing ... Total RNA was isolated using the RNeasy Kit (Qiagen) and included lysing in 350 µL RLT lysis buffer and the optional DNase treatment. cDNA libraries were prepared from 50 ng of total RNA and indexed with Illumina’s TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 and RS-122-2002).

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: RNA-seq libraries were constructed as previously described in Sultan et al., 2012 [ ] with minor modifications. .. Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev.

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: Paragraph title: RNA-Seq ... RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001).

    Article Title: Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
    Article Snippet: .. RNA-seq libraries were generated using TruSeq RNA library prep kit (Illumina, RS-122-2001/2) per the manufacturer’s instructions. .. The libraries were sequenced as 100bp single end using a HiSeq 2500 sequencer (Illumina).

    Article Title: Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye
    Article Snippet: .. RNA-Seq library preps were made using the Illumina TruSeq RNA sample Prep Kit v2 (Cat #RS-122-2002), using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50. .. For each RNA-seq sample, sequence quality was assessed with FastQC ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc ) and sequencing adapters were removed with Trimmomatic [ ].

    Article Title: Identification of a unique gene expression signature in mercury and 2,3,7,8-tetrachlorodibenzo-p-dioxin co-exposed cells
    Article Snippet: .. RNA-Seq libraries were prepared using an Illumina TruSeq RNA Sample Preparation Kit (RS-122-2002) according to the manufacturer’s protocol. .. Sequencing was performed as described earlier , using an Illumina HiSeq 2500 to obtain 50-nucleotide single-end reads.

    Magnetic Beads:

    Article Title: The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
    Article Snippet: RNA libraries were prepared using Illumina TruSeq RNA Sample Prep LT version 2 (RS-122-2001/2002). .. 1 μ g of total RNA was PolyA enriched using AMPure XP Magnetic Beads (Beckman Coulter #A63880).

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev. .. Further steps: purification, end-repair, A-tailing and adapter ligation were performed as described in the previously mentioned TruSeq kit protocol with one modification: the first purification eluate was not decanted from the magnetic beads and subsequent steps were performed with the beads in solution.

    Isolation:

    Article Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
    Article Snippet: Total RNA extraction from purified islet is described above. mRNA from whole islets was used to generate libraries using Illumina TruSeq RNA Sample Prep v2 (RS-122-2001). .. NEXSON ChIP-seq workflow ( ) is applied to freshly isolated mouse islets.

    Article Title: Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels
    Article Snippet: .. Total RNA was isolated using the RNeasy Kit (Qiagen) and included lysing in 350 µL RLT lysis buffer and the optional DNase treatment. cDNA libraries were prepared from 50 ng of total RNA and indexed with Illumina’s TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 and RS-122-2002). ..

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: Paragraph title: RNA isolation, library construction and deep-sequencing ... Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev.

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: 2.3 RNA-Seq Total RNA was isolated from NHEK cells by Qiazol lysis reagent (Qiagen, Hilden, Germany) and purified with miRNeasy mini kit (Qiagen) following manufacturer's instructions. .. RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001).

    Article Title: Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
    Article Snippet: RNA was isolated from 5 matched samples of in vitro differentiated control/CD33 KO human HSPC using Ambion RiboPure RNA purification kit (Thermo Fisher Scientific, AM1924). .. RNA-seq libraries were generated using TruSeq RNA library prep kit (Illumina, RS-122-2001/2) per the manufacturer’s instructions.

    Article Title: Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera
    Article Snippet: .. cDNA library construction for de novo Sequencing Tissues from 10 seedlings (3 month old whole seedling) were pooled together and RNA was isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA) and subsequently used to construct library with TruSeq RNA Sample preparation kit V2 (Illumina, Cat RS-122-2002) following manufactures protocol. .. 1 μg of total RNA by Qubit was enriched for Poly A using RNA Purification Beads.

    Article Title: Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye
    Article Snippet: RNA-Seq Larval tissue was isolated from 30 animals of each genotype in triplicate and RNA was extracted in Trizol (Invitrogen). .. RNA-Seq library preps were made using the Illumina TruSeq RNA sample Prep Kit v2 (Cat #RS-122-2002), using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50.

    Article Title: Identification of a unique gene expression signature in mercury and 2,3,7,8-tetrachlorodibenzo-p-dioxin co-exposed cells
    Article Snippet: Paragraph title: 2.2 RNA isolation and RNA-Seq ... RNA-Seq libraries were prepared using an Illumina TruSeq RNA Sample Preparation Kit (RS-122-2002) according to the manufacturer’s protocol.

    Multiplex Assay:

    Article Title: Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
    Article Snippet: For ES and iPS cells, we prepared samples for sequencing using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001). .. The HiSeq 2000 SR multiplex recipe was used.

    Article Title: Canonical and Noncanonical Actions of Arabidopsis Histone Deacetylases in Ribosomal RNA Processing
    Article Snippet: .. RNA-seq and ChIP-seq libraries were constructed using a TruSeq RNA Library Preparation Kit (Illumina; RS-122-2002) and the Ovation Ultralow DR Multiplex System (NuGEN; 0330), respectively. ..

    Purification:

    Article Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
    Article Snippet: .. Total RNA extraction from purified islet is described above. mRNA from whole islets was used to generate libraries using Illumina TruSeq RNA Sample Prep v2 (RS-122-2001). .. The manufacturer’s recommendations were followed and the libraries were sequenced on an Illumina HiSeq 2500 sequencer.

    Article Title: The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
    Article Snippet: Transcriptome Illumina Paired-End Library Construction and Sequencing Extracted, purified RNA was quantified and analyzed using a Qubit 2.0 (Life Technology, Carlsbad CA) and an Agilent Bioanalyzer 2100 (Agilent Technology, Santa Clara, CA). .. RNA libraries were prepared using Illumina TruSeq RNA Sample Prep LT version 2 (RS-122-2001/2002).

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: RNA extraction, cDNA library preparation and RNAseq Total RNA was purified from 27 samples [(untreated controls, 4 h nitrate, P-deficiency) × (three biological replicates) × (nodules from three plants to be pooled for one biological treatment)] using the TRIzol protocol (Invitrogen, Frankfurt, Germany). .. Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA.

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev. .. Further steps: purification, end-repair, A-tailing and adapter ligation were performed as described in the previously mentioned TruSeq kit protocol with one modification: the first purification eluate was not decanted from the magnetic beads and subsequent steps were performed with the beads in solution.

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: 2.3 RNA-Seq Total RNA was isolated from NHEK cells by Qiazol lysis reagent (Qiagen, Hilden, Germany) and purified with miRNeasy mini kit (Qiagen) following manufacturer's instructions. .. RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001).

    Article Title: Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
    Article Snippet: RNA was isolated from 5 matched samples of in vitro differentiated control/CD33 KO human HSPC using Ambion RiboPure RNA purification kit (Thermo Fisher Scientific, AM1924). .. RNA-seq libraries were generated using TruSeq RNA library prep kit (Illumina, RS-122-2001/2) per the manufacturer’s instructions.

    Article Title: Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera
    Article Snippet: cDNA library construction for de novo Sequencing Tissues from 10 seedlings (3 month old whole seedling) were pooled together and RNA was isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA) and subsequently used to construct library with TruSeq RNA Sample preparation kit V2 (Illumina, Cat RS-122-2002) following manufactures protocol. .. 1 μg of total RNA by Qubit was enriched for Poly A using RNA Purification Beads.

    Article Title: Transposable elements modulate human RNA abundance and splicing via specific RNA-protein interactions
    Article Snippet: Paragraph title: RNA purification and library construction ... To remove ribosomal RNA, we fed ≥70 ng of input and fRIP RNA into the Ribo-Zero™ Magnetic Gold Kit (Epicentre; Madison, WI, USA; catalog number MRZG12324) followed by a cleanup using Agencourt RNAClean XP beads (Beckman Coulter; Brea, CA, USA; catalog number A63987) and elution with 19.5 μl of Elute, Prime, Fragment mix from the TruSeq RNA Sample Preparation Kit (Illumina; San Diego, CA, USA; catalog number RS-122-2001).

    Polymerase Chain Reaction:

    Article Title: Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
    Article Snippet: Then 10 cycles of polymerase chain reaction (PCR) were performed to amplify the selected fragments using the Illumina supplied PCR primers and protocol. .. For ES and iPS cells, we prepared samples for sequencing using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001).

    Article Title: Recent polyploidization events in three Saccharum founding species
    Article Snippet: A pair‐end library for LA Purple was made using Illumina® TruSeq™ RNA Sample Preparation Kit (RS‐122‐2001(2), Illumina). .. A preprocessing quality control filter was imposed for both quality ( > 30) and depth of coverage ( > 7) to remove false positives due to PCR duplicates and low‐quality reads.

    Article Title: The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
    Article Snippet: RNA libraries were prepared using Illumina TruSeq RNA Sample Prep LT version 2 (RS-122-2001/2002). .. Illumina Reagents (Part #15012995) were used for PCR amplification.

    Article Title: H3K4 Methylation-Dependent Memory of Somatic Cell Identity Inhibits Reprogramming and Development of Nuclear Transfer Embryos
    Article Snippet: .. Per sample, 500 ng RNA was used to generate a cDNA sequencing libraries using a Illumina TrueSeq kit (RS-122-2001), according to the manufacturer’s protocol using 12 PCR amplification cycles. .. cDNA synthesis and RTqPCR analysis cDNA synthesis was performed from the isolated RNAs using oligo dT(15) primers.

    Article Title: Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye
    Article Snippet: .. RNA-Seq library preps were made using the Illumina TruSeq RNA sample Prep Kit v2 (Cat #RS-122-2002), using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50. .. For each RNA-seq sample, sequence quality was assessed with FastQC ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc ) and sequencing adapters were removed with Trimmomatic [ ].

    cDNA Library Assay:

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: Paragraph title: RNA extraction, cDNA library preparation and RNAseq ... Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA.

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001). .. In brief, 1 μg of total RNA from each sample was used to construct a cDNA library, followed by sequencing on the Illumina HiSeq 2500 system with single end 50 bp reads and ~30 millions of reads per sample.

    Article Title: Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera
    Article Snippet: .. cDNA library construction for de novo Sequencing Tissues from 10 seedlings (3 month old whole seedling) were pooled together and RNA was isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA) and subsequently used to construct library with TruSeq RNA Sample preparation kit V2 (Illumina, Cat RS-122-2002) following manufactures protocol. .. 1 μg of total RNA by Qubit was enriched for Poly A using RNA Purification Beads.

    Chromatin Immunoprecipitation:

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA. .. The size range of the final cDNA libraries was determined by applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (Böblingen, Germany) (280 bp).

    RNA Extraction:

    Article Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
    Article Snippet: .. Total RNA extraction from purified islet is described above. mRNA from whole islets was used to generate libraries using Illumina TruSeq RNA Sample Prep v2 (RS-122-2001). .. The manufacturer’s recommendations were followed and the libraries were sequenced on an Illumina HiSeq 2500 sequencer.

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: Paragraph title: RNA extraction, cDNA library preparation and RNAseq ... Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA.

    Sample Prep:

    Article Title: Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
    Article Snippet: .. For ES and iPS cells, we prepared samples for sequencing using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001). .. The samples were quantitated with Life Technologies Qubit fluorometer (Q32857).

    Article Title: Recent polyploidization events in three Saccharum founding species
    Article Snippet: .. A pair‐end library for LA Purple was made using Illumina® TruSeq™ RNA Sample Preparation Kit (RS‐122‐2001(2), Illumina). .. The library samples were sequenced on the Illumina HiSeq 2000 with 120 cycles by the KECK centre in UIUC ( http://www.biotech.uiuc.edu/ ).

    Article Title: The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes
    Article Snippet: .. Total RNA extraction from purified islet is described above. mRNA from whole islets was used to generate libraries using Illumina TruSeq RNA Sample Prep v2 (RS-122-2001). .. The manufacturer’s recommendations were followed and the libraries were sequenced on an Illumina HiSeq 2500 sequencer.

    Article Title: The Draft Genome and Transcriptome of the Atlantic Horseshoe Crab, Limulus polyphemus
    Article Snippet: .. RNA libraries were prepared using Illumina TruSeq RNA Sample Prep LT version 2 (RS-122-2001/2002). .. 1 μ g of total RNA was PolyA enriched using AMPure XP Magnetic Beads (Beckman Coulter #A63880).

    Article Title: Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels
    Article Snippet: .. Total RNA was isolated using the RNeasy Kit (Qiagen) and included lysing in 350 µL RLT lysis buffer and the optional DNase treatment. cDNA libraries were prepared from 50 ng of total RNA and indexed with Illumina’s TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 and RS-122-2002). ..

    Article Title: Nitrate application or P deficiency induce a decline in Medicago truncatula N2-fixation by similar changes in the nodule transcriptome
    Article Snippet: .. Library preparation for RNAseq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA. .. Accurate quantitation of cDNA libraries was performed using the QuantiFluor™ dsDNA System (Promega, Mannheim, Germany).

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: .. RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001). .. Transcriptome libraries were constructed by polyA purification.

    Article Title: Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera
    Article Snippet: .. cDNA library construction for de novo Sequencing Tissues from 10 seedlings (3 month old whole seedling) were pooled together and RNA was isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA) and subsequently used to construct library with TruSeq RNA Sample preparation kit V2 (Illumina, Cat RS-122-2002) following manufactures protocol. .. 1 μg of total RNA by Qubit was enriched for Poly A using RNA Purification Beads.

    Article Title: Transposable elements modulate human RNA abundance and splicing via specific RNA-protein interactions
    Article Snippet: .. To remove ribosomal RNA, we fed ≥70 ng of input and fRIP RNA into the Ribo-Zero™ Magnetic Gold Kit (Epicentre; Madison, WI, USA; catalog number MRZG12324) followed by a cleanup using Agencourt RNAClean XP beads (Beckman Coulter; Brea, CA, USA; catalog number A63987) and elution with 19.5 μl of Elute, Prime, Fragment mix from the TruSeq RNA Sample Preparation Kit (Illumina; San Diego, CA, USA; catalog number RS-122-2001). ..

    Article Title: Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye
    Article Snippet: .. RNA-Seq library preps were made using the Illumina TruSeq RNA sample Prep Kit v2 (Cat #RS-122-2002), using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50. .. For each RNA-seq sample, sequence quality was assessed with FastQC ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc ) and sequencing adapters were removed with Trimmomatic [ ].

    Article Title: Identification of a unique gene expression signature in mercury and 2,3,7,8-tetrachlorodibenzo-p-dioxin co-exposed cells
    Article Snippet: .. RNA-Seq libraries were prepared using an Illumina TruSeq RNA Sample Preparation Kit (RS-122-2002) according to the manufacturer’s protocol. .. Sequencing was performed as described earlier , using an Illumina HiSeq 2500 to obtain 50-nucleotide single-end reads.

    In Vitro:

    Article Title: Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
    Article Snippet: RNA was isolated from 5 matched samples of in vitro differentiated control/CD33 KO human HSPC using Ambion RiboPure RNA purification kit (Thermo Fisher Scientific, AM1924). .. RNA-seq libraries were generated using TruSeq RNA library prep kit (Illumina, RS-122-2001/2) per the manufacturer’s instructions.

    Next-Generation Sequencing:

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: .. RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001). .. Transcriptome libraries were constructed by polyA purification.

    Incubation:

    Article Title: Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system
    Article Snippet: Fragmentation and first strand cDNA synthesis were performed as in TruSeq RNA Library Prep kit v2 protocol (Illumina, RS-122-2001, instruction number 15026495 Rev. .. For second strand synthesis, reaction mixtures were supplemented with 1 μl of 5x First Strand Synthesis Buffer (18080–085, Thermo Fisher Scientific), 15 μl 5x Second Strand Synthesis Buffer (10812–014, Thermo Fisher Scientific), 0.45 μl 50 mM MgCl, 1 μl 100 mM DTT, 2 μl of 10 mM dUNTP Mix (dATP, dGTP, dCTP, dUTP, 10 mM each, R0182, R0133, Thermo Fisher Scientific), water to 57 μl, 5 U E . coli DNA Ligase (M0205L, NEB), 20 U E . coli DNA Polymerase I (NEB, M0209L), 1 U RNase H (18021–071, Thermo Fisher Scientific), and incubated at 16°C for 2h.

    Concentration Assay:

    Article Title: Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
    Article Snippet: Samples were loaded on the flowcell cluster station at a concentration of 8 pM, and sequenced on the Illumina GAII. .. For ES and iPS cells, we prepared samples for sequencing using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001).

    Lysis:

    Article Title: Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels
    Article Snippet: .. Total RNA was isolated using the RNeasy Kit (Qiagen) and included lysing in 350 µL RLT lysis buffer and the optional DNase treatment. cDNA libraries were prepared from 50 ng of total RNA and indexed with Illumina’s TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 and RS-122-2002). ..

    Article Title: Cannabidiol induces antioxidant pathways in keratinocytes by targeting BACH1
    Article Snippet: 2.3 RNA-Seq Total RNA was isolated from NHEK cells by Qiazol lysis reagent (Qiagen, Hilden, Germany) and purified with miRNeasy mini kit (Qiagen) following manufacturer's instructions. .. RNA was processed for high throughput sequencing using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001).

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    Illumina Inc truseq rna sample prep v2
    Truseq Rna Sample Prep V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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