truseq dna pcr free ht library preparation kit  (Illumina Inc)

 
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    TruSeq DNA PCR Free High Throughput Library Prep Kit
    Description:
    TruSeq DNA PCR Free provides simple all inclusive library preparation for whole genome sequencing applications Researchers can sequence a wide variety of organisms from small genomes such as bacteria to human whole genomes The workflows offer Shortened gel free workflows that remove the need for PCR Ability to sequence challenging regions Excellent coverage quality for deep insight into the genome Sequence challenging regions Within our whole genome sequencing workflows TruSeq DNA PCR Free offers superior coverage of areas that are traditionally difficult to sequence such as GC rich regions promoters and repetitive content The workflows are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments This permits the researcher to tailor each run to the needs of the experiment Gain deep insight into the genome PCR free means reduced library bias and gaps The result is high data quality and optimal variant detection across the genome Excellent genome coverage quality means your results have few gaps and good coverage of GC rich regions Save time with a PCR free protocol Removing PCR saves time and removes genomic coverage bias associated with PCR steps Bead based size selection shortens the workflow In tandem with Illumina sequencing systems TruSeq DNA PCR Free provides a range of enhancements to a widely adopted library preparation workflow Access flexible throughput options TruSeq DNA PCR Free with Single Indexes supports 24 sample manual processing for low throughput LT studies TruSeq DNA PCR Free with 96 CD Indexes supports 96 sample processing for high throughput HT studies and can be automated on liquid handling robots or processed manually IDT for Illumina Unique Dual UD Indexes 24 or 96 offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell Find a list of automation vendors with robotic systems that support the HT workflows
    Catalog Number:
    20015963
    Price:
    None
    Category:
    Library Preparation Kits
    Buy from Supplier


    Structured Review

    Illumina Inc truseq dna pcr free ht library preparation kit
    TruSeq DNA PCR Free High Throughput Library Prep Kit
    TruSeq DNA PCR Free provides simple all inclusive library preparation for whole genome sequencing applications Researchers can sequence a wide variety of organisms from small genomes such as bacteria to human whole genomes The workflows offer Shortened gel free workflows that remove the need for PCR Ability to sequence challenging regions Excellent coverage quality for deep insight into the genome Sequence challenging regions Within our whole genome sequencing workflows TruSeq DNA PCR Free offers superior coverage of areas that are traditionally difficult to sequence such as GC rich regions promoters and repetitive content The workflows are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments This permits the researcher to tailor each run to the needs of the experiment Gain deep insight into the genome PCR free means reduced library bias and gaps The result is high data quality and optimal variant detection across the genome Excellent genome coverage quality means your results have few gaps and good coverage of GC rich regions Save time with a PCR free protocol Removing PCR saves time and removes genomic coverage bias associated with PCR steps Bead based size selection shortens the workflow In tandem with Illumina sequencing systems TruSeq DNA PCR Free provides a range of enhancements to a widely adopted library preparation workflow Access flexible throughput options TruSeq DNA PCR Free with Single Indexes supports 24 sample manual processing for low throughput LT studies TruSeq DNA PCR Free with 96 CD Indexes supports 96 sample processing for high throughput HT studies and can be automated on liquid handling robots or processed manually IDT for Illumina Unique Dual UD Indexes 24 or 96 offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell Find a list of automation vendors with robotic systems that support the HT workflows
    https://www.bioz.com/result/truseq dna pcr free ht library preparation kit/product/Illumina Inc
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    truseq dna pcr free ht library preparation kit - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Performance of four modern whole genome amplification methods for copy number variant detection in single cells"

    Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-03711-y

    Experimental design. Samples consisting of 1, 3 or 5 cells were collected from the Loucy cell line using micromanipulation for each WGA method in triplicate. Cells were amplified with either Ampli-1, REPLI-g or DOPlify, followed by PCR-free Illumina library preparation and sequencing. A fourth method, Picoseq, performs WGA and library preparation simultaneously, without the need for a separate library preparation. A bulk DNA sample was extracted from 5 * 10 6 Loucy cells using a column-based extraction method from Qiagen, also followed by PCR-free Illumina library preparation and sequencing.
    Figure Legend Snippet: Experimental design. Samples consisting of 1, 3 or 5 cells were collected from the Loucy cell line using micromanipulation for each WGA method in triplicate. Cells were amplified with either Ampli-1, REPLI-g or DOPlify, followed by PCR-free Illumina library preparation and sequencing. A fourth method, Picoseq, performs WGA and library preparation simultaneously, without the need for a separate library preparation. A bulk DNA sample was extracted from 5 * 10 6 Loucy cells using a column-based extraction method from Qiagen, also followed by PCR-free Illumina library preparation and sequencing.

    Techniques Used: Micromanipulation, Whole Genome Amplification, Amplification, Polymerase Chain Reaction, Sequencing

    2) Product Images from "STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods"

    Article Title: STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-17525-5

    Experimental design. Cells from the Loucy cell line were preserved for 24 hours in Cell-Free DNA BCT reagent. Samples consisting of 1- or 3-cells were isolated from this fixed cell suspension for each WGA method. Ampli1, DOPlify, PicoPLEX and REPLI-g were used for amplification, followed by Illumina PCR-Free library preparation and next generation sequencing. In parallel, STR-PCR and capillary electrophoresis was performed on all samples, including a bulk sample from the cell line.
    Figure Legend Snippet: Experimental design. Cells from the Loucy cell line were preserved for 24 hours in Cell-Free DNA BCT reagent. Samples consisting of 1- or 3-cells were isolated from this fixed cell suspension for each WGA method. Ampli1, DOPlify, PicoPLEX and REPLI-g were used for amplification, followed by Illumina PCR-Free library preparation and next generation sequencing. In parallel, STR-PCR and capillary electrophoresis was performed on all samples, including a bulk sample from the cell line.

    Techniques Used: Isolation, Whole Genome Amplification, Amplification, Polymerase Chain Reaction, Next-Generation Sequencing, Electrophoresis

    Related Articles

    Amplification:

    Article Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots
    Article Snippet: .. High-throughput sequencing and data processing Pooled DNA samples from the Pbx1 recombination assay were prepared for sequencing using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) in order to avoid PCR amplification, which could lead to template switching during amplification, in turn leading to false recombinant molecules. .. After library preparation Pbx1 DNA was size-selected using the Pippin Prep (Sage Science).

    Sample Prep:

    Article Title: A Balance of Yki/Sd Activator and E2F1/Sd Repressor Complexes Controls Cell Survival and Affects Organ Size
    Article Snippet: .. The sequencing library was prepared using a TruSeq DNA PCR-Free Sample Preparation kit (Illumina). ..

    Article Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots
    Article Snippet: .. High-throughput sequencing and data processing Pooled DNA samples from the Pbx1 recombination assay were prepared for sequencing using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) in order to avoid PCR amplification, which could lead to template switching during amplification, in turn leading to false recombinant molecules. .. After library preparation Pbx1 DNA was size-selected using the Pippin Prep (Sage Science).

    Article Title: CRISPR/Cas9-Mediated Genome Editing as a Therapeutic Approach for Leber Congenital Amaurosis 10
    Article Snippet: .. The DNA samples were fragmented to an average target fragment size of 350 bp by ultrasonication and were used to construct a sequencing library using the Illumina TruSeq DNA PCR-free sample preparation kit. .. The library was quantified and assessed using a Qubit and 2100 Bioanalyzer, after which the samples were loaded onto an Illumina platform to generate PE150 reads.

    Polymerase Chain Reaction:

    Article Title: FANCM Limits Meiotic Crossovers in Brassica Crops
    Article Snippet: .. Whole genome libraries were prepared using the TruSeq® DNA PCR-Free LT kit (Illumina). .. Briefly, sample preparation was performed with the low sample protocol using a 550 bp fragment sizing; all enzymatic steps and clean-up procedures were performed according to manufacturer's instructions.

    Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells
    Article Snippet: .. Sequencing libraries of the fragmented samples were prepared with the TruSeq DNA PCR-free HT library preparation kit (Illumina) on the IP-Star Compact (Diagenode, Seraing, Belgium). .. Library quantification was performed using a Sequencing Library qPCR Quantification kit (Illumina, San Diego, USA) to quantify the sequenceable DNA fragments containing the correct adapters (this was performed on the samples of all four methods).

    Article Title: Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA
    Article Snippet: .. Illumina TruSeq DNA PCR-Free LT Library Preparation Kit was obtained from Illumina (San Diego, CA) and Kapa Hyper Prep Kit was obtained from Kapa Biosystems (Wilmington, MA). .. Human DNA was obtained from Promega (Madison, WI).

    Article Title: A Balance of Yki/Sd Activator and E2F1/Sd Repressor Complexes Controls Cell Survival and Affects Organ Size
    Article Snippet: .. The sequencing library was prepared using a TruSeq DNA PCR-Free Sample Preparation kit (Illumina). ..

    Article Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots
    Article Snippet: .. High-throughput sequencing and data processing Pooled DNA samples from the Pbx1 recombination assay were prepared for sequencing using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) in order to avoid PCR amplification, which could lead to template switching during amplification, in turn leading to false recombinant molecules. .. After library preparation Pbx1 DNA was size-selected using the Pippin Prep (Sage Science).

    Article Title: CRISPR/Cas9-Mediated Genome Editing as a Therapeutic Approach for Leber Congenital Amaurosis 10
    Article Snippet: .. The DNA samples were fragmented to an average target fragment size of 350 bp by ultrasonication and were used to construct a sequencing library using the Illumina TruSeq DNA PCR-free sample preparation kit. .. The library was quantified and assessed using a Qubit and 2100 Bioanalyzer, after which the samples were loaded onto an Illumina platform to generate PE150 reads.

    Article Title: Design and Experimental Evaluation of a Minimal, Innocuous Watermarking Strategy to Distinguish Near-Identical DNA and RNA Sequences
    Article Snippet: .. DNA libraries were prepared using the TruSeq DNA PCR-Free Library Preparation Kit (Illumina) according to the manufacturer’s manual. .. Quantification of the libraries was done by qPCR using the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems, Wilmington, MA, USA) on a Rotor-Gene Q PCR cycler (Qiagen).

    Construct:

    Article Title: CRISPR/Cas9-Mediated Genome Editing as a Therapeutic Approach for Leber Congenital Amaurosis 10
    Article Snippet: .. The DNA samples were fragmented to an average target fragment size of 350 bp by ultrasonication and were used to construct a sequencing library using the Illumina TruSeq DNA PCR-free sample preparation kit. .. The library was quantified and assessed using a Qubit and 2100 Bioanalyzer, after which the samples were loaded onto an Illumina platform to generate PE150 reads.

    Sequencing:

    Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells
    Article Snippet: .. Sequencing libraries of the fragmented samples were prepared with the TruSeq DNA PCR-free HT library preparation kit (Illumina) on the IP-Star Compact (Diagenode, Seraing, Belgium). .. Library quantification was performed using a Sequencing Library qPCR Quantification kit (Illumina, San Diego, USA) to quantify the sequenceable DNA fragments containing the correct adapters (this was performed on the samples of all four methods).

    Article Title: A Balance of Yki/Sd Activator and E2F1/Sd Repressor Complexes Controls Cell Survival and Affects Organ Size
    Article Snippet: .. The sequencing library was prepared using a TruSeq DNA PCR-Free Sample Preparation kit (Illumina). ..

    Article Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots
    Article Snippet: .. High-throughput sequencing and data processing Pooled DNA samples from the Pbx1 recombination assay were prepared for sequencing using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) in order to avoid PCR amplification, which could lead to template switching during amplification, in turn leading to false recombinant molecules. .. After library preparation Pbx1 DNA was size-selected using the Pippin Prep (Sage Science).

    Article Title: CRISPR/Cas9-Mediated Genome Editing as a Therapeutic Approach for Leber Congenital Amaurosis 10
    Article Snippet: .. The DNA samples were fragmented to an average target fragment size of 350 bp by ultrasonication and were used to construct a sequencing library using the Illumina TruSeq DNA PCR-free sample preparation kit. .. The library was quantified and assessed using a Qubit and 2100 Bioanalyzer, after which the samples were loaded onto an Illumina platform to generate PE150 reads.

    Recombination Assay:

    Article Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots
    Article Snippet: .. High-throughput sequencing and data processing Pooled DNA samples from the Pbx1 recombination assay were prepared for sequencing using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) in order to avoid PCR amplification, which could lead to template switching during amplification, in turn leading to false recombinant molecules. .. After library preparation Pbx1 DNA was size-selected using the Pippin Prep (Sage Science).

    High Throughput Screening Assay:

    Article Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots
    Article Snippet: .. High-throughput sequencing and data processing Pooled DNA samples from the Pbx1 recombination assay were prepared for sequencing using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) in order to avoid PCR amplification, which could lead to template switching during amplification, in turn leading to false recombinant molecules. .. After library preparation Pbx1 DNA was size-selected using the Pippin Prep (Sage Science).

    Recombinant:

    Article Title: Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots
    Article Snippet: .. High-throughput sequencing and data processing Pooled DNA samples from the Pbx1 recombination assay were prepared for sequencing using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) in order to avoid PCR amplification, which could lead to template switching during amplification, in turn leading to false recombinant molecules. .. After library preparation Pbx1 DNA was size-selected using the Pippin Prep (Sage Science).

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    Illumina Inc truseq dna pcr free ht library preparation kit
    Experimental design. Samples consisting of 1, 3 or 5 cells were collected from the Loucy cell line using micromanipulation for each WGA method in triplicate. Cells were amplified with either Ampli-1, REPLI-g or DOPlify, followed by <t>PCR-free</t> Illumina library preparation and sequencing. A fourth method, Picoseq, performs WGA and library preparation simultaneously, without the need for a separate library preparation. A bulk <t>DNA</t> sample was extracted from 5 * 10 6 Loucy cells using a column-based extraction method from Qiagen, also followed by PCR-free Illumina library preparation and sequencing.
    Truseq Dna Pcr Free Ht Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq dna pcr free ht library preparation kit/product/Illumina Inc
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    truseq dna pcr free ht library preparation kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Experimental design. Samples consisting of 1, 3 or 5 cells were collected from the Loucy cell line using micromanipulation for each WGA method in triplicate. Cells were amplified with either Ampli-1, REPLI-g or DOPlify, followed by PCR-free Illumina library preparation and sequencing. A fourth method, Picoseq, performs WGA and library preparation simultaneously, without the need for a separate library preparation. A bulk DNA sample was extracted from 5 * 10 6 Loucy cells using a column-based extraction method from Qiagen, also followed by PCR-free Illumina library preparation and sequencing.

    Journal: Scientific Reports

    Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells

    doi: 10.1038/s41598-017-03711-y

    Figure Lengend Snippet: Experimental design. Samples consisting of 1, 3 or 5 cells were collected from the Loucy cell line using micromanipulation for each WGA method in triplicate. Cells were amplified with either Ampli-1, REPLI-g or DOPlify, followed by PCR-free Illumina library preparation and sequencing. A fourth method, Picoseq, performs WGA and library preparation simultaneously, without the need for a separate library preparation. A bulk DNA sample was extracted from 5 * 10 6 Loucy cells using a column-based extraction method from Qiagen, also followed by PCR-free Illumina library preparation and sequencing.

    Article Snippet: Sequencing libraries of the fragmented samples were prepared with the TruSeq DNA PCR-free HT library preparation kit (Illumina) on the IP-Star Compact (Diagenode, Seraing, Belgium).

    Techniques: Micromanipulation, Whole Genome Amplification, Amplification, Polymerase Chain Reaction, Sequencing

    Experimental design. Cells from the Loucy cell line were preserved for 24 hours in Cell-Free DNA BCT reagent. Samples consisting of 1- or 3-cells were isolated from this fixed cell suspension for each WGA method. Ampli1, DOPlify, PicoPLEX and REPLI-g were used for amplification, followed by Illumina PCR-Free library preparation and next generation sequencing. In parallel, STR-PCR and capillary electrophoresis was performed on all samples, including a bulk sample from the cell line.

    Journal: Scientific Reports

    Article Title: STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods

    doi: 10.1038/s41598-017-17525-5

    Figure Lengend Snippet: Experimental design. Cells from the Loucy cell line were preserved for 24 hours in Cell-Free DNA BCT reagent. Samples consisting of 1- or 3-cells were isolated from this fixed cell suspension for each WGA method. Ampli1, DOPlify, PicoPLEX and REPLI-g were used for amplification, followed by Illumina PCR-Free library preparation and next generation sequencing. In parallel, STR-PCR and capillary electrophoresis was performed on all samples, including a bulk sample from the cell line.

    Article Snippet: Subsequently, library preparation, library quantification and single-end indexed 75 bp sequencing was performed as earlier clarified by Deleye et al ., respectively using the TruSeq DNA PCR-free HT library preparation kit (Illumina, San Diego, USA), the Sequencing Library qPCR Quantification kit (Illumina, San Diego, USA) and a high-output NextSeq.

    Techniques: Isolation, Whole Genome Amplification, Amplification, Polymerase Chain Reaction, Next-Generation Sequencing, Electrophoresis