trueview  (Vector Laboratories)


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    Name:
    Vector TrueVIEW Autofluorescence Quenching Kit
    Description:
    The Vector TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non lipofuscin sources and dramatically improve signal to noise ratio It removes unwanted autofluorescence in tissue sections due to aldehyde fixation red blood cells and structural elements such as collagen and elastin The quenching action of the kit reagents provides a clear unambiguous “true view localization of the target antigen
    Catalog Number:
    SP-8400
    Price:
    None
    Category:
    Protein chemifluorescent detection reagents or kits or substrates
    Size:
    15 ml
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    Structured Review

    Vector Laboratories trueview
    The Vector TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non lipofuscin sources and dramatically improve signal to noise ratio It removes unwanted autofluorescence in tissue sections due to aldehyde fixation red blood cells and structural elements such as collagen and elastin The quenching action of the kit reagents provides a clear unambiguous “true view localization of the target antigen
    https://www.bioz.com/result/trueview/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trueview - by Bioz Stars, 2021-06
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses
    Article Snippet: Immunostaining was performed on 3 μm-thick sections using antibodies against GFP (ICL lab), Alexa Fluor 647-directly labeled B220 (eBioscience) and Lectin PNA Alexa Fluor 488 Conjugate (ThermoFisher). .. Immunostained slides were incubated with the Vector® TrueVIEW Autofluorescence Quenching Kit to remove autofluorescence signal (Vector Laboratories). .. Stained sections were scanned using a Panoramic Digital Slide Scanner 250 FLASH II (3DHISTECH) at 200 x magnification.

    Plasmid Preparation:

    Article Title: Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses
    Article Snippet: Immunostaining was performed on 3 μm-thick sections using antibodies against GFP (ICL lab), Alexa Fluor 647-directly labeled B220 (eBioscience) and Lectin PNA Alexa Fluor 488 Conjugate (ThermoFisher). .. Immunostained slides were incubated with the Vector® TrueVIEW Autofluorescence Quenching Kit to remove autofluorescence signal (Vector Laboratories). .. Stained sections were scanned using a Panoramic Digital Slide Scanner 250 FLASH II (3DHISTECH) at 200 x magnification.

    Article Title: Histone deacetylases 1 and 2 silence cryptic transcription to promote mitochondrial function during cardiogenesis
    Article Snippet: Eosin Y and Harris modified hematoxylin were obtained from Fisher, while X-gal was purchased from 5-Prime. .. Vectashield permanent mounting medium, Vectashield fluorescent mounting medium, Vectashield Elite ABC reagent kit, Vector TrueVIEW Autofluorescence Quenching Kit, and DAB Peroxidase Substrate kit were purchased from Vector Labs. .. ProLong Glass Antifade Mounting Medium was purchased from Thermo Fisher Scientific.

    Article Title: Presence of Clock genes in equine full-term placenta
    Article Snippet: .. Sections were rinsed three times with 1× PBS, then quenched for 5 min at room temperature with hydrogen peroxide (H2 O2) and Vector TrueView Autofluorescence Quenching Kit. ..

    Article Title: Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background
    Article Snippet: Then, sections were washed in PBS for 3 × 5 min. After the last wash step secondary antibodies diluted in blocking buffer were added for additional 30 min incubation, RT. .. A second wash with PBS for 3 × 5 min, RT, was conducted prior to the use of a second auto fluorescence quenching kit (Vector TrueVIEW autofluorescence quenching kit, Vector Laboratories, # SP-8400). .. Finally, sections were washed in PBS for 5 min, incubated with DAPI (conc.

    Immunofluorescence:

    Article Title: Heme activates platelets and exacerbates rhabdomyolysis-induced acute kidney injury via CLEC-2 and GPVI/FcRγ
    Article Snippet: We incubated specimens with 1:200 rabbit anti-CitH3 antibody (Ab5103, Abcam, Cambridge, MA) and 1:200 rat anti-F4/80 antibody (eBioscience, San Diego, CA) at 4°C overnight and then with 1:1000 Alexa Fluor 555–labeled anti-rabbit IgG antibody (Abcam) and 1:500 Alexa Fluor 488–labeled anti–rat IgG antibody (Life Technologies, Carlsbad, CA) for 90 minutes. .. After immunofluorescence staining, autofluorescence was suppressed by using TrueVIEW (Vector Laboratories, Burlingame, CA). .. Finally, nuclear staining was performed with 5 µg/mL 4′,6-diamidino-2-phenylindole (Sigma-Aldrich) for 5 minutes.

    Staining:

    Article Title: Heme activates platelets and exacerbates rhabdomyolysis-induced acute kidney injury via CLEC-2 and GPVI/FcRγ
    Article Snippet: We incubated specimens with 1:200 rabbit anti-CitH3 antibody (Ab5103, Abcam, Cambridge, MA) and 1:200 rat anti-F4/80 antibody (eBioscience, San Diego, CA) at 4°C overnight and then with 1:1000 Alexa Fluor 555–labeled anti-rabbit IgG antibody (Abcam) and 1:500 Alexa Fluor 488–labeled anti–rat IgG antibody (Life Technologies, Carlsbad, CA) for 90 minutes. .. After immunofluorescence staining, autofluorescence was suppressed by using TrueVIEW (Vector Laboratories, Burlingame, CA). .. Finally, nuclear staining was performed with 5 µg/mL 4′,6-diamidino-2-phenylindole (Sigma-Aldrich) for 5 minutes.

    Fluorescence:

    Article Title: Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background
    Article Snippet: Then, sections were washed in PBS for 3 × 5 min. After the last wash step secondary antibodies diluted in blocking buffer were added for additional 30 min incubation, RT. .. A second wash with PBS for 3 × 5 min, RT, was conducted prior to the use of a second auto fluorescence quenching kit (Vector TrueVIEW autofluorescence quenching kit, Vector Laboratories, # SP-8400). .. Finally, sections were washed in PBS for 5 min, incubated with DAPI (conc.

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    Vector Laboratories dapi
    TRAIL induces apoptosis in adult patient-derived glioma cells but not in hADSCs. Glioma cells transfected with NPs/pTRAIL exhibited obvious <t>TUNEL-positive</t> staining, indicating that the majority of cells underwent apoptosis 48 h after transfection. In contrast, hADSCs transfected with NPs/pTRAIL or NPs/pCDNA exhibited negligible apoptotic activity. Similarly, glioma cells transfected with NPs/pCDNA also remained viable. Blue indicates <t>DAPI</t> counterstain for nuclei and green indicates TUNEL immunostaining of cell apoptosis. (Scale bars, 50 μm.)
    Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dapi - by Bioz Stars, 2021-06
    99/100 stars
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    97
    Vector Laboratories detection kits
    TRAIL induces apoptosis in adult patient-derived glioma cells but not in hADSCs. Glioma cells transfected with NPs/pTRAIL exhibited obvious <t>TUNEL-positive</t> staining, indicating that the majority of cells underwent apoptosis 48 h after transfection. In contrast, hADSCs transfected with NPs/pTRAIL or NPs/pCDNA exhibited negligible apoptotic activity. Similarly, glioma cells transfected with NPs/pCDNA also remained viable. Blue indicates <t>DAPI</t> counterstain for nuclei and green indicates TUNEL immunostaining of cell apoptosis. (Scale bars, 50 μm.)
    Detection Kits, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/detection kits/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    detection kits - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    Image Search Results


    TRAIL induces apoptosis in adult patient-derived glioma cells but not in hADSCs. Glioma cells transfected with NPs/pTRAIL exhibited obvious TUNEL-positive staining, indicating that the majority of cells underwent apoptosis 48 h after transfection. In contrast, hADSCs transfected with NPs/pTRAIL or NPs/pCDNA exhibited negligible apoptotic activity. Similarly, glioma cells transfected with NPs/pCDNA also remained viable. Blue indicates DAPI counterstain for nuclei and green indicates TUNEL immunostaining of cell apoptosis. (Scale bars, 50 μm.)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Nanoparticle engineered TRAIL-overexpressing adipose-derived stem cells target and eradicate glioblastoma via intracranial delivery

    doi: 10.1073/pnas.1615396113

    Figure Lengend Snippet: TRAIL induces apoptosis in adult patient-derived glioma cells but not in hADSCs. Glioma cells transfected with NPs/pTRAIL exhibited obvious TUNEL-positive staining, indicating that the majority of cells underwent apoptosis 48 h after transfection. In contrast, hADSCs transfected with NPs/pTRAIL or NPs/pCDNA exhibited negligible apoptotic activity. Similarly, glioma cells transfected with NPs/pCDNA also remained viable. Blue indicates DAPI counterstain for nuclei and green indicates TUNEL immunostaining of cell apoptosis. (Scale bars, 50 μm.)

    Article Snippet: To detect in vivo apoptotic activity, tumor-bearing brain sections were stained with a TUNEL assay kit (Roche) and mounted with Vectashield mounting medium with DAPI (Vector Lab).

    Techniques: Derivative Assay, Transfection, TUNEL Assay, Staining, Activity Assay, Immunostaining

    Regulation of Akt activity modulates SMC CREB content. Rat PA SMCs were grown in complete medium and stably transfected with a vector expressing wild-type CREB linked to ECFP. After selection, rapidly growing colonies exhibiting high levels of ECFP fluorescence were pooled and expanded. These cells were infected with adenoviruses expressing dominant negative Akt-AAA, constitutively active myr-Akt, or LacZ (Cntrl) at a multiplicity of infection of 100. Some cells also received CREB-specific siRNA as indicated. Twenty-four hours later, the cells were transferred to DMEM containing 0.2% FCS and incubated overnight before the addition of PDGF to a concentration of 25 ng/ml. Duplicate plates were left untreated as a control (Cntrl). Medium and PDGF were replaced every 24 h for 72 h. (A) Cells were fixed in PBS with 4% paraformaldehyde, and coverslips were affixed with VectaShield with DAPI to visualize nuclei. The figure shows representative fluorescence deconvolution images of DAPI or ECFP fluorescence at a magnification of ×416. (B) Cell proliferation was measured with CellTiter 96 AQ reagents. Open bars, untreated; cross-hatched bars, PDGF; solid bars, CREB siRNA.

    Journal: Molecular and Cellular Biology

    Article Title: Platelet-Derived Growth Factor BB Induces Nuclear Export and Proteasomal Degradation of CREB via Phosphatidylinositol 3-Kinase/Akt Signaling in Pulmonary Artery Smooth Muscle Cells

    doi: 10.1128/MCB.02477-05

    Figure Lengend Snippet: Regulation of Akt activity modulates SMC CREB content. Rat PA SMCs were grown in complete medium and stably transfected with a vector expressing wild-type CREB linked to ECFP. After selection, rapidly growing colonies exhibiting high levels of ECFP fluorescence were pooled and expanded. These cells were infected with adenoviruses expressing dominant negative Akt-AAA, constitutively active myr-Akt, or LacZ (Cntrl) at a multiplicity of infection of 100. Some cells also received CREB-specific siRNA as indicated. Twenty-four hours later, the cells were transferred to DMEM containing 0.2% FCS and incubated overnight before the addition of PDGF to a concentration of 25 ng/ml. Duplicate plates were left untreated as a control (Cntrl). Medium and PDGF were replaced every 24 h for 72 h. (A) Cells were fixed in PBS with 4% paraformaldehyde, and coverslips were affixed with VectaShield with DAPI to visualize nuclei. The figure shows representative fluorescence deconvolution images of DAPI or ECFP fluorescence at a magnification of ×416. (B) Cell proliferation was measured with CellTiter 96 AQ reagents. Open bars, untreated; cross-hatched bars, PDGF; solid bars, CREB siRNA.

    Article Snippet: Vector NovaRed peroxidase staining kits, VectaShield mounting medium with DAPI (4′,6′-diamidino-2-phenylindole), and peroxidase-conjugated goat anti-rabbit secondary antibody were from Vector Laboratories (Burlingame, CA).

    Techniques: Activity Assay, Stable Transfection, Transfection, Plasmid Preparation, Expressing, Selection, Fluorescence, Infection, Dominant Negative Mutation, Incubation, Concentration Assay

    PDGF-induced CREB depletion occurs via phosphorylation of CREB serines 103 and 107. Wild-type (WT) CREB-327 and CREB mutants containing serine-to-alanine mutations at serines 115, 119, and 138 and a dual mutation of serines 103 and 107 were linked in frame to ECFP in the plasmid pECFP-C1. Plasmids were stably transfected into rat PA SMCs as described in Materials and Methods. After selection in G418, rapidly growing colonies exhibiting bright ECFP fluorescence were pooled and expanded. The cells were plated on an eight-well microscope slide at 20% confluence. Some cells were infected with an adenovirus expressing constitutively active myr-Akt at a multiplicity of infection of 100 as indicated. The cells were transferred to DMEM containing 0.2% FCS for 24 h, and PDGF was added to a concentration of 25 ng/ml. Medium and PDGF was replaced every 24 h for 72 h. Duplicate wells were left untreated as a control. (A) Cells were fixed and coverslips were attached with VectaShield plus DAPI to visualize nuclei. The figure shows representative fluorescence deconvolution images of DAPI and ECFP fluorescence at a magnification of ×400. (B) Duplicate plates of cells harvested and separated into cytosolic (“C”) and nuclear (“N”) fractions, which were resolved on 10% polyacrylamide-SDS gels and transferred to PVDF membranes. The figure shows a representative Western blot for ECFP-CREB and a Coomassie blue-stained gel as a loading control. (C) Whole-cell lysates (25 μg protein) from cells expressing the indicated ECFP-CREB isoforms were resolved on polyacrylamide-SDS gels and transferred to PVDF membranes. The figure shows a representative long-exposure Western blot for ECFP-CREB and a Coomassie blue-stained gel as a loading control. The positions of ECFP-CREB and ubiquitinated ECFP-CREB (Ub-ECFP-CREB) are indicated in the figure.

    Journal: Molecular and Cellular Biology

    Article Title: Platelet-Derived Growth Factor BB Induces Nuclear Export and Proteasomal Degradation of CREB via Phosphatidylinositol 3-Kinase/Akt Signaling in Pulmonary Artery Smooth Muscle Cells

    doi: 10.1128/MCB.02477-05

    Figure Lengend Snippet: PDGF-induced CREB depletion occurs via phosphorylation of CREB serines 103 and 107. Wild-type (WT) CREB-327 and CREB mutants containing serine-to-alanine mutations at serines 115, 119, and 138 and a dual mutation of serines 103 and 107 were linked in frame to ECFP in the plasmid pECFP-C1. Plasmids were stably transfected into rat PA SMCs as described in Materials and Methods. After selection in G418, rapidly growing colonies exhibiting bright ECFP fluorescence were pooled and expanded. The cells were plated on an eight-well microscope slide at 20% confluence. Some cells were infected with an adenovirus expressing constitutively active myr-Akt at a multiplicity of infection of 100 as indicated. The cells were transferred to DMEM containing 0.2% FCS for 24 h, and PDGF was added to a concentration of 25 ng/ml. Medium and PDGF was replaced every 24 h for 72 h. Duplicate wells were left untreated as a control. (A) Cells were fixed and coverslips were attached with VectaShield plus DAPI to visualize nuclei. The figure shows representative fluorescence deconvolution images of DAPI and ECFP fluorescence at a magnification of ×400. (B) Duplicate plates of cells harvested and separated into cytosolic (“C”) and nuclear (“N”) fractions, which were resolved on 10% polyacrylamide-SDS gels and transferred to PVDF membranes. The figure shows a representative Western blot for ECFP-CREB and a Coomassie blue-stained gel as a loading control. (C) Whole-cell lysates (25 μg protein) from cells expressing the indicated ECFP-CREB isoforms were resolved on polyacrylamide-SDS gels and transferred to PVDF membranes. The figure shows a representative long-exposure Western blot for ECFP-CREB and a Coomassie blue-stained gel as a loading control. The positions of ECFP-CREB and ubiquitinated ECFP-CREB (Ub-ECFP-CREB) are indicated in the figure.

    Article Snippet: Vector NovaRed peroxidase staining kits, VectaShield mounting medium with DAPI (4′,6′-diamidino-2-phenylindole), and peroxidase-conjugated goat anti-rabbit secondary antibody were from Vector Laboratories (Burlingame, CA).

    Techniques: Mutagenesis, Plasmid Preparation, Stable Transfection, Transfection, Selection, Fluorescence, Microscopy, Infection, Expressing, Concentration Assay, Western Blot, Staining