trpv1  (Alomone Labs)


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    Structured Review

    Alomone Labs trpv1
    Immunofluorescence staining of <t>TRPV1,</t> Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups
    Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpv1/product/Alomone Labs
    Average 96 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    trpv1 - by Bioz Stars, 2022-08
    96/100 stars

    Images

    1) Product Images from "Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice"

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00451-0

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    TRPV1 and related molecular pathways in the mouse brain
    Figure Legend Snippet: TRPV1 and related molecular pathways in the mouse brain

    Techniques Used:

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups
    Figure Legend Snippet: Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups

    Techniques Used: Mouse Assay

    Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    2) Product Images from "TRPV1 neurons regulate β-cell function in a sex-dependent manner"

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2018.10.002

    Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).
    Figure Legend Snippet: Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Techniques Used: Cell Function Assay, Mouse Assay, Injection, Fluorescence, Staining

    Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).
    Figure Legend Snippet: Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Techniques Used: Mouse Assay, Injection

    Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).
    Figure Legend Snippet: Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Techniques Used: Mouse Assay, Injection, Activity Assay

    3) Product Images from "Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice"

    Article Title: Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000264

    NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P
    Figure Legend Snippet: NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P

    Techniques Used: Expressing, Western Blot, Mouse Assay, Imaging, Labeling, Injection, CtB Assay, Functional Assay

    4) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    5) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    6) Product Images from "Transient receptor potential V1 modulates neuroinflammation in Parkinson’s disease dementia: Molecular implications for electroacupuncture and rivastigmine"

    Article Title: Transient receptor potential V1 modulates neuroinflammation in Parkinson’s disease dementia: Molecular implications for electroacupuncture and rivastigmine

    Journal: Iranian Journal of Basic Medical Sciences

    doi: 10.22038/IJBMS.2021.56156.12531

    Immunofluorescence staining of TRPV1 protein in the hippocampal CA1 area. Con: Control, PDD: Parkinson’s disease dementia, EA: PDD + EA, Riva: PDD + rivastigmine. Each group n= 3. Scale bar in the right lower corner of each picture represents 50 µm. White arrows indicate TRPV1-positive neurons
    Figure Legend Snippet: Immunofluorescence staining of TRPV1 protein in the hippocampal CA1 area. Con: Control, PDD: Parkinson’s disease dementia, EA: PDD + EA, Riva: PDD + rivastigmine. Each group n= 3. Scale bar in the right lower corner of each picture represents 50 µm. White arrows indicate TRPV1-positive neurons

    Techniques Used: Immunofluorescence, Staining

    TRPV1 and related molecular pathways
    Figure Legend Snippet: TRPV1 and related molecular pathways

    Techniques Used:

    Expression levels of TRPV1-associated signaling pathways in the mice hippocampus. (A) TRPV1, (B) pPKA, (C) pPI3K, (D) pPKC, (E) pAkt, (F) pmTOR, (G) pERK, (H) pCREB, (I) α7 nicotinic receptor, and (J) Parvalbumin expression levels in Con, PDD, EA, Riva. Con: normal mice; PDD: Parkinson’s disease dementia mice; EA: PDD+ EA. Riva: PDD + oral rivastigmine. Each group n= 6
    Figure Legend Snippet: Expression levels of TRPV1-associated signaling pathways in the mice hippocampus. (A) TRPV1, (B) pPKA, (C) pPI3K, (D) pPKC, (E) pAkt, (F) pmTOR, (G) pERK, (H) pCREB, (I) α7 nicotinic receptor, and (J) Parvalbumin expression levels in Con, PDD, EA, Riva. Con: normal mice; PDD: Parkinson’s disease dementia mice; EA: PDD+ EA. Riva: PDD + oral rivastigmine. Each group n= 6

    Techniques Used: Expressing, Mouse Assay

    Expression levels of TRPV1-associated signaling pathways in the mice prefrontal cortex. (A) TRPV1, (B) pPKA, (C) pPI3K, (D) pPKC, (E) pAkt, (F) pmTOR, (G) pERK, (H) pCREB, (I) α7 nicotinic receptor, and (J) Parvalbumin expression levels in Con, PDD, EA, Riva. Con: normal mice; PDD: Parkinson’s disease dementia mice; EA: PDD + EA. Riva: PDD + oral rivastigmine. Each group n= 6
    Figure Legend Snippet: Expression levels of TRPV1-associated signaling pathways in the mice prefrontal cortex. (A) TRPV1, (B) pPKA, (C) pPI3K, (D) pPKC, (E) pAkt, (F) pmTOR, (G) pERK, (H) pCREB, (I) α7 nicotinic receptor, and (J) Parvalbumin expression levels in Con, PDD, EA, Riva. Con: normal mice; PDD: Parkinson’s disease dementia mice; EA: PDD + EA. Riva: PDD + oral rivastigmine. Each group n= 6

    Techniques Used: Expressing, Mouse Assay

    Immunofluorescence staining of TRPV1 protein expression in the prefrontal cortex. Con: Control, PDD: Parkinson’s disease dementia, EA: PDD + EA, Riva: PDD + rivastigmine. Each group n= 3. Scale bar (in the right lower part of each picture) is 50 µm. The white arrows indicate TRPV1-positive neurons
    Figure Legend Snippet: Immunofluorescence staining of TRPV1 protein expression in the prefrontal cortex. Con: Control, PDD: Parkinson’s disease dementia, EA: PDD + EA, Riva: PDD + rivastigmine. Each group n= 3. Scale bar (in the right lower part of each picture) is 50 µm. The white arrows indicate TRPV1-positive neurons

    Techniques Used: Immunofluorescence, Staining, Expressing

    7) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    8) Product Images from "Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice"

    Article Title: Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000264

    NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P
    Figure Legend Snippet: NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P

    Techniques Used: Expressing, Western Blot, Mouse Assay, Imaging, Labeling, Injection, CtB Assay, Functional Assay

    9) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    10) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    11) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    12) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    13) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    14) Product Images from "Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice"

    Article Title: Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000264

    NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P
    Figure Legend Snippet: NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P

    Techniques Used: Expressing, Western Blot, Mouse Assay, Imaging, Labeling, Injection, CtB Assay, Functional Assay

    15) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    16) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    17) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    18) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    19) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    20) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    21) Product Images from "TRPV1 neurons regulate β-cell function in a sex-dependent manner"

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2018.10.002

    Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).
    Figure Legend Snippet: Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Techniques Used: Cell Function Assay, Mouse Assay, Injection, Fluorescence, Staining

    Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).
    Figure Legend Snippet: Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Techniques Used: Mouse Assay, Injection

    Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).
    Figure Legend Snippet: Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Techniques Used: Mouse Assay, Injection, Activity Assay

    22) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    23) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    24) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    25) Product Images from "Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine"

    Article Title: Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.00356.2012

    Intraluminal transient receptor potential (TRP) vanilloid type 1 (TRPV1) and TRP ankyrin 1 (TRPA1) agonists increase blood flow in the small intestine. Capsaicin (CAP, 3 mg/kg body wt) or allyl isothiocyanate (AITC, 0.002 mg/kg body wt) was administered
    Figure Legend Snippet: Intraluminal transient receptor potential (TRP) vanilloid type 1 (TRPV1) and TRP ankyrin 1 (TRPA1) agonists increase blood flow in the small intestine. Capsaicin (CAP, 3 mg/kg body wt) or allyl isothiocyanate (AITC, 0.002 mg/kg body wt) was administered

    Techniques Used: Flow Cytometry

    TRPA1 and ADM expression in intestinal epithelial cells. A : RT-PCR analysis was performed for TRPA1, TRPV1, ADM, and β-actin in rat intestinal epithelial (IE) cells of the small intestine (S-IE), those of the large intestine (L-IE), dorsal root
    Figure Legend Snippet: TRPA1 and ADM expression in intestinal epithelial cells. A : RT-PCR analysis was performed for TRPA1, TRPV1, ADM, and β-actin in rat intestinal epithelial (IE) cells of the small intestine (S-IE), those of the large intestine (L-IE), dorsal root

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

    26) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    27) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    28) Product Images from "TRPV1 Responses in the Cerebellum Lobules VI, VII, VIII Using Electroacupuncture Treatment for Chronic Pain and Depression Comorbidity in a Murine Model"

    Article Title: TRPV1 Responses in the Cerebellum Lobules VI, VII, VIII Using Electroacupuncture Treatment for Chronic Pain and Depression Comorbidity in a Murine Model

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22095028

    Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VIa and VIb. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. ( A ) The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobule VIa. ( B ) Conversely, no significant variance in pNFkB density was observed in cerebellum lobule VIb among the 5 groups, although TRPV1 maintained an analogous trend of decreased expression in the AS and AS + SHAM group, which was absent in the AS + KO group and increased in the AS + EA group when compared to Control. Scale bar is 50 μm.
    Figure Legend Snippet: Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VIa and VIb. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. ( A ) The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobule VIa. ( B ) Conversely, no significant variance in pNFkB density was observed in cerebellum lobule VIb among the 5 groups, although TRPV1 maintained an analogous trend of decreased expression in the AS and AS + SHAM group, which was absent in the AS + KO group and increased in the AS + EA group when compared to Control. Scale bar is 50 μm.

    Techniques Used: Immunofluorescence, Staining, Expressing

    Illustration of CPDC pathways attenuated by EA in the cerebellum. We found that EA at 1 mA, 2 Hz/20 min, and TRPV1 gene deletion can increase the expressions of TRPV1, Nav1.7, Nav1.8, GABAAα1, NMDAR1, and TrkB receptors in the AS-induced CPDC model, as observed in the AS + EA and AS + KO groups. Furthermore, the relevant increases in related responses cause effective increases in the signaling of pPI3K, pAkt, pERK, pmTOR, pPKCε, pPKAIIα, pNFκB, and pCREB under conditions of CPDC, and these serve to present a basic neuromodulatory pathway of CPDC, TRPV1 signaling, and the molecular function of EA.
    Figure Legend Snippet: Illustration of CPDC pathways attenuated by EA in the cerebellum. We found that EA at 1 mA, 2 Hz/20 min, and TRPV1 gene deletion can increase the expressions of TRPV1, Nav1.7, Nav1.8, GABAAα1, NMDAR1, and TrkB receptors in the AS-induced CPDC model, as observed in the AS + EA and AS + KO groups. Furthermore, the relevant increases in related responses cause effective increases in the signaling of pPI3K, pAkt, pERK, pmTOR, pPKCε, pPKAIIα, pNFκB, and pCREB under conditions of CPDC, and these serve to present a basic neuromodulatory pathway of CPDC, TRPV1 signaling, and the molecular function of EA.

    Techniques Used:

    The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VIII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly augmented in the AS + EA and AS + KO groups. Both the AS + EA and AS + KO groups displayed no difference when compared to the Control group, depicting an observable improvement of CPDC tendencies. However, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of significant increases in the AS and AS + SHAM groups when compared to the Control group. Furthermore, this increase was significantly ameliorated in the AS + EA and AS + KO groups, which retained states of non-significance when similarly compared to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p
    Figure Legend Snippet: The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VIII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly augmented in the AS + EA and AS + KO groups. Both the AS + EA and AS + KO groups displayed no difference when compared to the Control group, depicting an observable improvement of CPDC tendencies. However, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of significant increases in the AS and AS + SHAM groups when compared to the Control group. Furthermore, this increase was significantly ameliorated in the AS + EA and AS + KO groups, which retained states of non-significance when similarly compared to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p

    Techniques Used: Expressing

    The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB and ( J ) pNFκB ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Interestingly, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of no significant variances across all 5 groups. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p
    Figure Legend Snippet: The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB and ( J ) pNFκB ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Interestingly, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of no significant variances across all 5 groups. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p

    Techniques Used: Expressing

    The expression levels of nociceptive receptors and associated molecules in cerebellum lobule VI. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Conversely, the protein expressions of the AS and AS + SHAM groups were significantly increased in ( C ) Nav1.7 and ( D ) Nav1.8 when compared to the Control group. Correspondingly, the AS + EA and AS + KO groups were augmented and showed no significant difference in comparison to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p
    Figure Legend Snippet: The expression levels of nociceptive receptors and associated molecules in cerebellum lobule VI. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Conversely, the protein expressions of the AS and AS + SHAM groups were significantly increased in ( C ) Nav1.7 and ( D ) Nav1.8 when compared to the Control group. Correspondingly, the AS + EA and AS + KO groups were augmented and showed no significant difference in comparison to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p

    Techniques Used: Expressing

    Comparative graph of paw withdrawal threshold and latency of the Acid-Saline (AS)-induced chronic pain subjects after electroacupuncture (EA) treatment and transient receptor vanilloid member 1 (TRPV1) gene deletion (KO). Control, AS, AS + EA, AS + SHAM, and AS + KO were tested according to ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ nociceptive sensitivities. * p
    Figure Legend Snippet: Comparative graph of paw withdrawal threshold and latency of the Acid-Saline (AS)-induced chronic pain subjects after electroacupuncture (EA) treatment and transient receptor vanilloid member 1 (TRPV1) gene deletion (KO). Control, AS, AS + EA, AS + SHAM, and AS + KO were tested according to ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ nociceptive sensitivities. * p

    Techniques Used:

    Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VII and VIII. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobules ( A ) VII and ( B ) VIII. Scale bar is 50 μm.
    Figure Legend Snippet: Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VII and VIII. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobules ( A ) VII and ( B ) VIII. Scale bar is 50 μm.

    Techniques Used: Immunofluorescence, Staining, Expressing

    29) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    30) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    31) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    32) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    33) Product Images from "TRPV1 neurons regulate β-cell function in a sex-dependent manner"

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2018.10.002

    Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).
    Figure Legend Snippet: Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Techniques Used: Cell Function Assay, Mouse Assay, Injection, Fluorescence, Staining

    Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).
    Figure Legend Snippet: Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Techniques Used: Mouse Assay, Injection

    Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).
    Figure Legend Snippet: Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Techniques Used: Mouse Assay, Injection, Activity Assay

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    Alomone Labs trpv1
    Immunofluorescence staining of <t>TRPV1,</t> Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups
    Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpv1/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv1 - by Bioz Stars, 2022-08
    96/100 stars
      Buy from Supplier

    96
    Alomone Labs rabbit trpv1
    Ciliary neurotrophic factor (CNTF) has a neuroprotective effect on dopaminergic neurons. Nam et al. report that the capsaicin receptor <t>TRPV1</t> expressed on astrocytes mediates the production of endogenous CNTF to inhibit degeneration of dopaminergic neurons in two rodent models of Parkinson's disease.
    Rabbit Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit trpv1/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit trpv1 - by Bioz Stars, 2022-08
    96/100 stars
      Buy from Supplier

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    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    TRPV1 and related molecular pathways in the mouse brain

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: TRPV1 and related molecular pathways in the mouse brain

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques:

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay

    Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    Ciliary neurotrophic factor (CNTF) has a neuroprotective effect on dopaminergic neurons. Nam et al. report that the capsaicin receptor TRPV1 expressed on astrocytes mediates the production of endogenous CNTF to inhibit degeneration of dopaminergic neurons in two rodent models of Parkinson's disease.

    Journal: Brain

    Article Title: TRPV1 on astrocytes rescues nigral dopamine neurons in Parkinson’s disease via CNTF

    doi: 10.1093/brain/awv297

    Figure Lengend Snippet: Ciliary neurotrophic factor (CNTF) has a neuroprotective effect on dopaminergic neurons. Nam et al. report that the capsaicin receptor TRPV1 expressed on astrocytes mediates the production of endogenous CNTF to inhibit degeneration of dopaminergic neurons in two rodent models of Parkinson's disease.

    Article Snippet: The following primary antibodies and dilutions were used: rabbit TRPV1 (1:1000, Alomone labs), mouse anti-CNTF (1:1000, Millipore), mouse anti-GFAP (1:500, Sigma), mouse anti-tyrosine hydroxylase (TH, 1:1000, Millipore), rabbit anti-phosphorylation Ser31 tyrosine hydroxylase (1:1000, Millipore), and mouse anti-beta-actin (1:5000, Abcam).

    Techniques:

    TRPV1 expression in the substantia nigra of MPP + -lesioned rat. The rats were given a unilateral medial forebrain bundle (MFB) injection of MPP + and brain tissues were processed for immunohistochemical and western blot analysis at 1 week post MPP + . ( A–D ) Fluorescence images of TRPV1 (magenta; A ) and GFAP (green; A ), or TRPV1 (magenta; B ) and TH (green; B ) or TRPV1 (magenta; C ) and OX-42 (green; C ) and both images are merged (yellow; A–C ) in the SNpc of MPP + -lesioned rat brain. ( D ) Quantification of TRPV1 expression in each cell type, * P

    Journal: Brain

    Article Title: TRPV1 on astrocytes rescues nigral dopamine neurons in Parkinson’s disease via CNTF

    doi: 10.1093/brain/awv297

    Figure Lengend Snippet: TRPV1 expression in the substantia nigra of MPP + -lesioned rat. The rats were given a unilateral medial forebrain bundle (MFB) injection of MPP + and brain tissues were processed for immunohistochemical and western blot analysis at 1 week post MPP + . ( A–D ) Fluorescence images of TRPV1 (magenta; A ) and GFAP (green; A ), or TRPV1 (magenta; B ) and TH (green; B ) or TRPV1 (magenta; C ) and OX-42 (green; C ) and both images are merged (yellow; A–C ) in the SNpc of MPP + -lesioned rat brain. ( D ) Quantification of TRPV1 expression in each cell type, * P

    Article Snippet: The following primary antibodies and dilutions were used: rabbit TRPV1 (1:1000, Alomone labs), mouse anti-CNTF (1:1000, Millipore), mouse anti-GFAP (1:500, Sigma), mouse anti-tyrosine hydroxylase (TH, 1:1000, Millipore), rabbit anti-phosphorylation Ser31 tyrosine hydroxylase (1:1000, Millipore), and mouse anti-beta-actin (1:5000, Abcam).

    Techniques: Expressing, Injection, Immunohistochemistry, Western Blot, Fluorescence

    Astrocytic TRPV1 induced CNTF exerts neuroprotection on dopamine neurons in vivo. ( A ) Diagram of the experimental design showing various treatments as indicated. After the last rotation experiment, brain tissues were prepared for immunohistochemical staining or western blot analysis. ( B ) Photomicrographs of TH + cells in the SN and TH + fibres in the striatum (STR). ( C ) Number of TH + or Nissl + cells in the SNpc, and optical density of TH + fibres in the striatum, * P

    Journal: Brain

    Article Title: TRPV1 on astrocytes rescues nigral dopamine neurons in Parkinson’s disease via CNTF

    doi: 10.1093/brain/awv297

    Figure Lengend Snippet: Astrocytic TRPV1 induced CNTF exerts neuroprotection on dopamine neurons in vivo. ( A ) Diagram of the experimental design showing various treatments as indicated. After the last rotation experiment, brain tissues were prepared for immunohistochemical staining or western blot analysis. ( B ) Photomicrographs of TH + cells in the SN and TH + fibres in the striatum (STR). ( C ) Number of TH + or Nissl + cells in the SNpc, and optical density of TH + fibres in the striatum, * P

    Article Snippet: The following primary antibodies and dilutions were used: rabbit TRPV1 (1:1000, Alomone labs), mouse anti-CNTF (1:1000, Millipore), mouse anti-GFAP (1:500, Sigma), mouse anti-tyrosine hydroxylase (TH, 1:1000, Millipore), rabbit anti-phosphorylation Ser31 tyrosine hydroxylase (1:1000, Millipore), and mouse anti-beta-actin (1:5000, Abcam).

    Techniques: In Vivo, Immunohistochemistry, Staining, Western Blot

    Expression of TH, TRPV1, GFAP, CNTF and CNTFRα in the substantia nigra of human Parkinson’s disease brain. ( A–F ) Western blot analysis of TH, TRPV1, GFAP, CNTF, and CNTFRα in the SN ( A–D ) or cortex ( E and F ) of human control and Parkinson’s disease brain. ( G–J ) Immunohistochemical analysis of TRPV1 (red, G ) or CNTF (red, I ) in GFAP + astrocytes (green, G and I ) in the SNpc of human control or Parkinson’s disease brain. DAPI (blue) stained nucleus. Quantification of TRPV1 ( H ) or CNTF ( J ) expression co-localized in GFAP + astrocytes in the SNpc of human brain, respectively ( K–O ) Photomicrographs of neuromelain + dopamine neurons (brown) and CNTFRα + cells (blue) in the SNpc of human control ( K and L ) and Parkinson’s disease ( M and N ) brain. L and N show enlargement of areas depicted in the boxes of K and M , respectively. Quantification of CNTFRα expression in each neuromelanin + dopamine neuron in the SNpc of human brain ( O ). One hundred and fifty-six (control) or 65 (Parkinson’s disease) neuromelanin + cells were analysed. * P

    Journal: Brain

    Article Title: TRPV1 on astrocytes rescues nigral dopamine neurons in Parkinson’s disease via CNTF

    doi: 10.1093/brain/awv297

    Figure Lengend Snippet: Expression of TH, TRPV1, GFAP, CNTF and CNTFRα in the substantia nigra of human Parkinson’s disease brain. ( A–F ) Western blot analysis of TH, TRPV1, GFAP, CNTF, and CNTFRα in the SN ( A–D ) or cortex ( E and F ) of human control and Parkinson’s disease brain. ( G–J ) Immunohistochemical analysis of TRPV1 (red, G ) or CNTF (red, I ) in GFAP + astrocytes (green, G and I ) in the SNpc of human control or Parkinson’s disease brain. DAPI (blue) stained nucleus. Quantification of TRPV1 ( H ) or CNTF ( J ) expression co-localized in GFAP + astrocytes in the SNpc of human brain, respectively ( K–O ) Photomicrographs of neuromelain + dopamine neurons (brown) and CNTFRα + cells (blue) in the SNpc of human control ( K and L ) and Parkinson’s disease ( M and N ) brain. L and N show enlargement of areas depicted in the boxes of K and M , respectively. Quantification of CNTFRα expression in each neuromelanin + dopamine neuron in the SNpc of human brain ( O ). One hundred and fifty-six (control) or 65 (Parkinson’s disease) neuromelanin + cells were analysed. * P

    Article Snippet: The following primary antibodies and dilutions were used: rabbit TRPV1 (1:1000, Alomone labs), mouse anti-CNTF (1:1000, Millipore), mouse anti-GFAP (1:500, Sigma), mouse anti-tyrosine hydroxylase (TH, 1:1000, Millipore), rabbit anti-phosphorylation Ser31 tyrosine hydroxylase (1:1000, Millipore), and mouse anti-beta-actin (1:5000, Abcam).

    Techniques: Expressing, Western Blot, Immunohistochemistry, Staining

    TRPV1 activation by dietary capsaicin attenuates endothelial oxidative stress and increases the level of NO in diabetic mice. A and B : Representative protein expressions of p22 phox ( A ) and p-eNOS ( B ) in aortas from db/db mice treated with normal diet (db/db Cont) or normal diet plus 0.01% capsaicin (db/db Cap) and wild type mice treated with normal diet (WT Cont). ## P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: TRPV1 activation by dietary capsaicin attenuates endothelial oxidative stress and increases the level of NO in diabetic mice. A and B : Representative protein expressions of p22 phox ( A ) and p-eNOS ( B ) in aortas from db/db mice treated with normal diet (db/db Cont) or normal diet plus 0.01% capsaicin (db/db Cap) and wild type mice treated with normal diet (WT Cont). ## P

    Article Snippet: The vessels were incubated with antibodies against TRPV1 (Alomone Labs, Israel), PKA or UCP2 (Santa Cruz Biotechnology, USA) overnight at 4°C and incubated with fluorescent dye-labeled secondary antibodies (ZSGB-BIO, China) at room temperature for 30 min.

    Techniques: Activation Assay, Mouse Assay

    Upregulation of UCP2 by TRPV1 activation attenuates hyperglycemia-induced ROS production in ECs. The increased metabolism of glucose due to intracellular hyperglycemia leads to the overproduction of NADH, a critical component of the superoxide-generating mechanism in endothelial cells. Upregulation of mitochondrial UCP2 in response to elevated superoxide levels plays an active role in the feedback regulation of reactive oxygen species production that is associated with chronic oxidative stress. Activation of the endothelial TRPV1 channel in endothelial cells by dietary capsaicin mediates the phosphorylation of PKA and upregulates UCP2, thus inhibiting the activity of NADPH and decreasing ROS production in ECs.

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: Upregulation of UCP2 by TRPV1 activation attenuates hyperglycemia-induced ROS production in ECs. The increased metabolism of glucose due to intracellular hyperglycemia leads to the overproduction of NADH, a critical component of the superoxide-generating mechanism in endothelial cells. Upregulation of mitochondrial UCP2 in response to elevated superoxide levels plays an active role in the feedback regulation of reactive oxygen species production that is associated with chronic oxidative stress. Activation of the endothelial TRPV1 channel in endothelial cells by dietary capsaicin mediates the phosphorylation of PKA and upregulates UCP2, thus inhibiting the activity of NADPH and decreasing ROS production in ECs.

    Article Snippet: The vessels were incubated with antibodies against TRPV1 (Alomone Labs, Israel), PKA or UCP2 (Santa Cruz Biotechnology, USA) overnight at 4°C and incubated with fluorescent dye-labeled secondary antibodies (ZSGB-BIO, China) at room temperature for 30 min.

    Techniques: Activation Assay, Activity Assay

    The effect of TRPV1 activation on the production of ROS and NO through the PKA/UCP2 pathway. A and B : Representative western blot images ( A ) and summary data ( B ) showing P22 phox protein level in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L), HG+Cap+Genipin (10 μmol/L). ## P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: The effect of TRPV1 activation on the production of ROS and NO through the PKA/UCP2 pathway. A and B : Representative western blot images ( A ) and summary data ( B ) showing P22 phox protein level in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L), HG+Cap+Genipin (10 μmol/L). ## P

    Article Snippet: The vessels were incubated with antibodies against TRPV1 (Alomone Labs, Israel), PKA or UCP2 (Santa Cruz Biotechnology, USA) overnight at 4°C and incubated with fluorescent dye-labeled secondary antibodies (ZSGB-BIO, China) at room temperature for 30 min.

    Techniques: Activation Assay, Western Blot, Cell Culture

    TRPV1 activation ameliorates high-glucose-induced endothelial dysfunction in a UCP2-dependent manner. A : Representative immunofluorescence images showing the co-expression of TRPV1, PKA and UCP2 in the aortas from wild type mice, particularly in the endothelium (Bar denotes 50 μm). B and C : Acetylcholine (1 nmol/L to 10 μmol/L)-induced endothelium-dependent relaxation of isolated aortic artery rings from wild type and TRPV1 -/- mice, pre-incubated with normal-glucose for 12 hours (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+KT5720 (2 μmol/L); **P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: TRPV1 activation ameliorates high-glucose-induced endothelial dysfunction in a UCP2-dependent manner. A : Representative immunofluorescence images showing the co-expression of TRPV1, PKA and UCP2 in the aortas from wild type mice, particularly in the endothelium (Bar denotes 50 μm). B and C : Acetylcholine (1 nmol/L to 10 μmol/L)-induced endothelium-dependent relaxation of isolated aortic artery rings from wild type and TRPV1 -/- mice, pre-incubated with normal-glucose for 12 hours (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+KT5720 (2 μmol/L); **P

    Article Snippet: The vessels were incubated with antibodies against TRPV1 (Alomone Labs, Israel), PKA or UCP2 (Santa Cruz Biotechnology, USA) overnight at 4°C and incubated with fluorescent dye-labeled secondary antibodies (ZSGB-BIO, China) at room temperature for 30 min.

    Techniques: Activation Assay, Immunofluorescence, Expressing, Mouse Assay, Isolation, Incubation

    TRPV1 activation by dietary capsaicin promotes endothelial PKA phosphorylation and increases UCP2 levels in diabetic mice. Representative protein expression of TRPV1 ( A and B ), p-PKA/PKA ( C and D ) and UCP2 ( E ) levels in aorta or mesenteric arteries from db/db mice treated with normal diet (db/db Cont) or normal diet plus 0.01% capsaicin (db/db Cap) and the lean littermate control C57BL/KsJ mice treated with normal diet (WT Cont). Data are mean ± SEM. Each n = 3. ## P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: TRPV1 activation by dietary capsaicin promotes endothelial PKA phosphorylation and increases UCP2 levels in diabetic mice. Representative protein expression of TRPV1 ( A and B ), p-PKA/PKA ( C and D ) and UCP2 ( E ) levels in aorta or mesenteric arteries from db/db mice treated with normal diet (db/db Cont) or normal diet plus 0.01% capsaicin (db/db Cap) and the lean littermate control C57BL/KsJ mice treated with normal diet (WT Cont). Data are mean ± SEM. Each n = 3. ## P

    Article Snippet: The vessels were incubated with antibodies against TRPV1 (Alomone Labs, Israel), PKA or UCP2 (Santa Cruz Biotechnology, USA) overnight at 4°C and incubated with fluorescent dye-labeled secondary antibodies (ZSGB-BIO, China) at room temperature for 30 min.

    Techniques: Activation Assay, Mouse Assay, Expressing

    Activation of TRPV1 up-regulates UCP2 through PKA phosphorylation. A , B and C : Representative western blot images showing protein expressions of TRPV1 ( A ), p-PKA/PKA ( B ) and UCP2 ( C ) in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L) or HG+Cap+Genipin (10 μmol/L); #P

    Journal: Cardiovascular Diabetology

    Article Title: TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction

    doi: 10.1186/1475-2840-12-69

    Figure Lengend Snippet: Activation of TRPV1 up-regulates UCP2 through PKA phosphorylation. A , B and C : Representative western blot images showing protein expressions of TRPV1 ( A ), p-PKA/PKA ( B ) and UCP2 ( C ) in endothelial cells cultured with normal-glucose (NG, glucose 5.5 mmol/L), high-glucose (HG, glucose 30 mmol/L), HG+capsaicin (HG+Cap, Cap 1 μmol/L), HG+Cap+5’-iodo-resiniferatoxin (HG+Cap+iRTX, iRTX 1 μmol/L), HG+Cap+KT5720 (2 μmol/L) or HG+Cap+Genipin (10 μmol/L); #P

    Article Snippet: The vessels were incubated with antibodies against TRPV1 (Alomone Labs, Israel), PKA or UCP2 (Santa Cruz Biotechnology, USA) overnight at 4°C and incubated with fluorescent dye-labeled secondary antibodies (ZSGB-BIO, China) at room temperature for 30 min.

    Techniques: Activation Assay, Western Blot, Cell Culture

    Expressions of TRPV1 and pERK in the hypothalamus of normal, CFA, CFA + 2 Hz EA, and CFA + sham EA. TRPV1-positive neurons (green) in the mPFC of ( A ) normal, ( B ) CFA, ( C ) CFA + 2 Hz EA, and ( D ) CFA + sham EA mice. pERK-positive neurons (green) in the mPFC of ( E ) normal, ( F ) CFA, ( G ) CFA + 2 Hz EA, and ( H ) CFA + sham EA mice. Scale bar means 50 m.

    Journal: International Journal of Molecular Sciences

    Article Title: Distal Electroacupuncture at the LI4 Acupoint Reduces CFA-Induced Inflammatory Pain via the Brain TRPV1 Signaling Pathway

    doi: 10.3390/ijms20184471

    Figure Lengend Snippet: Expressions of TRPV1 and pERK in the hypothalamus of normal, CFA, CFA + 2 Hz EA, and CFA + sham EA. TRPV1-positive neurons (green) in the mPFC of ( A ) normal, ( B ) CFA, ( C ) CFA + 2 Hz EA, and ( D ) CFA + sham EA mice. pERK-positive neurons (green) in the mPFC of ( E ) normal, ( F ) CFA, ( G ) CFA + 2 Hz EA, and ( H ) CFA + sham EA mice. Scale bar means 50 m.

    Article Snippet: The following primary antibodies were used: anti-TRPV1 (1:500, Alomone, Israel) and anti-pERK (1:500, Alomone, Israel) from Alomone.

    Techniques: Mouse Assay

    Schematic illustration of distal EA mechanisms of analgesia in CFA-induced inflammatory pain. Summary diagram of how distal EA and TRPV1 are crucial for inflammatory pain and related mechanisms. Our results suggest that distal EA can reduce inflammatory pain through brain mechanisms.

    Journal: International Journal of Molecular Sciences

    Article Title: Distal Electroacupuncture at the LI4 Acupoint Reduces CFA-Induced Inflammatory Pain via the Brain TRPV1 Signaling Pathway

    doi: 10.3390/ijms20184471

    Figure Lengend Snippet: Schematic illustration of distal EA mechanisms of analgesia in CFA-induced inflammatory pain. Summary diagram of how distal EA and TRPV1 are crucial for inflammatory pain and related mechanisms. Our results suggest that distal EA can reduce inflammatory pain through brain mechanisms.

    Article Snippet: The following primary antibodies were used: anti-TRPV1 (1:500, Alomone, Israel) and anti-pERK (1:500, Alomone, Israel) from Alomone.

    Techniques:

    Expressions of TRPV1 and pERK in the PAG of normal, CFA, CFA + 2 Hz EA, and CFA + sham EA. TRPV1-positive neurons (green) in the mPFC of ( A ) normal, ( B ) CFA, ( C ) CFA + 2 Hz EA, and ( D ) CFA + sham EA mice. pERK-positive neurons (green) in the mPFC of ( E ) normal, ( F ) CFA, ( G ) CFA + 2 Hz EA, and ( H ) CFA + sham EA mice. Scale bar means 50 m.

    Journal: International Journal of Molecular Sciences

    Article Title: Distal Electroacupuncture at the LI4 Acupoint Reduces CFA-Induced Inflammatory Pain via the Brain TRPV1 Signaling Pathway

    doi: 10.3390/ijms20184471

    Figure Lengend Snippet: Expressions of TRPV1 and pERK in the PAG of normal, CFA, CFA + 2 Hz EA, and CFA + sham EA. TRPV1-positive neurons (green) in the mPFC of ( A ) normal, ( B ) CFA, ( C ) CFA + 2 Hz EA, and ( D ) CFA + sham EA mice. pERK-positive neurons (green) in the mPFC of ( E ) normal, ( F ) CFA, ( G ) CFA + 2 Hz EA, and ( H ) CFA + sham EA mice. Scale bar means 50 m.

    Article Snippet: The following primary antibodies were used: anti-TRPV1 (1:500, Alomone, Israel) and anti-pERK (1:500, Alomone, Israel) from Alomone.

    Techniques: Mouse Assay

    Expression levels of transient receptor potential cation channel, subfamily V, member 1 (TRPV1)-associated signaling pathways in the mice medial prefrontal cortex (mPFC). ( A ) TRPV1, ( B ) pPKA, ( C ) pPI3K, ( D ) pPKCε, ( E ) pERK, ( F ) pp38, ( G ) phosphorylated c-Jun N-terminal kinase (pJNK), ( H ) phosphorylated protein kinase B (pAkt), ( I ) phosphorylated mammalian target of rapamycin (pmTOR), ( J ) phosphorylated nuclear factor κB (pNFκB), ( K ) voltage-gated sodium channel 1.7 (Nav1.7), and ( L ) voltage-gated sodium channel 1.8 (Nav1.8) expression levels in normal, CFA, CFA + 2 Hz EA, and CFA + sham EA (from left to right). Normal = normal mice; CFA = CFA-induced inflammatory pain; 2 Hz EA = CFA + 2 Hz EA. Sham EA = CFA + sham EA. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Distal Electroacupuncture at the LI4 Acupoint Reduces CFA-Induced Inflammatory Pain via the Brain TRPV1 Signaling Pathway

    doi: 10.3390/ijms20184471

    Figure Lengend Snippet: Expression levels of transient receptor potential cation channel, subfamily V, member 1 (TRPV1)-associated signaling pathways in the mice medial prefrontal cortex (mPFC). ( A ) TRPV1, ( B ) pPKA, ( C ) pPI3K, ( D ) pPKCε, ( E ) pERK, ( F ) pp38, ( G ) phosphorylated c-Jun N-terminal kinase (pJNK), ( H ) phosphorylated protein kinase B (pAkt), ( I ) phosphorylated mammalian target of rapamycin (pmTOR), ( J ) phosphorylated nuclear factor κB (pNFκB), ( K ) voltage-gated sodium channel 1.7 (Nav1.7), and ( L ) voltage-gated sodium channel 1.8 (Nav1.8) expression levels in normal, CFA, CFA + 2 Hz EA, and CFA + sham EA (from left to right). Normal = normal mice; CFA = CFA-induced inflammatory pain; 2 Hz EA = CFA + 2 Hz EA. Sham EA = CFA + sham EA. * p

    Article Snippet: The following primary antibodies were used: anti-TRPV1 (1:500, Alomone, Israel) and anti-pERK (1:500, Alomone, Israel) from Alomone.

    Techniques: Expressing, Mouse Assay

    Expressions of TRPV1 and pERK in the mPFC of normal, CFA, CFA + 2 Hz EA, and CFA + sham EA. TRPV1-positive neurons (green) in the mPFC of ( A ) normal, ( B ) CFA, ( C ) CFA + 2 Hz EA, and ( D ) CFA + sham EA mice. pERK-positive neurons (green) in the mPFC of ( E ) normal, ( F ) CFA, ( G ) CFA + 2 Hz EA, and ( H ) CFA + sham EA mice. Scale bar means 50 m.

    Journal: International Journal of Molecular Sciences

    Article Title: Distal Electroacupuncture at the LI4 Acupoint Reduces CFA-Induced Inflammatory Pain via the Brain TRPV1 Signaling Pathway

    doi: 10.3390/ijms20184471

    Figure Lengend Snippet: Expressions of TRPV1 and pERK in the mPFC of normal, CFA, CFA + 2 Hz EA, and CFA + sham EA. TRPV1-positive neurons (green) in the mPFC of ( A ) normal, ( B ) CFA, ( C ) CFA + 2 Hz EA, and ( D ) CFA + sham EA mice. pERK-positive neurons (green) in the mPFC of ( E ) normal, ( F ) CFA, ( G ) CFA + 2 Hz EA, and ( H ) CFA + sham EA mice. Scale bar means 50 m.

    Article Snippet: The following primary antibodies were used: anti-TRPV1 (1:500, Alomone, Israel) and anti-pERK (1:500, Alomone, Israel) from Alomone.

    Techniques: Mouse Assay

    Expression levels of TRPV1-associated signaling pathways in the mice hypothalamus. ( A ) TRPV1, ( B ) pPKA, ( C ) pPI3K, ( D ) pPKCε, ( E ) pERK, ( F ) pp38, ( G ) JNK, ( H ) pAkt, ( I ) pmTOR, ( J ) pNFκB, ( K ) Nav1.7, and ( L ) Nav1.8 expression levels in normal, CFA, CFA + 2 Hz EA, and CFA + sham EA (from left to right). Normal = normal mice; CFA = CFA-induced inflammatory pain; 2 Hz EA = CFA + 2H z EA. Sham EA = CFA + sham EA. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Distal Electroacupuncture at the LI4 Acupoint Reduces CFA-Induced Inflammatory Pain via the Brain TRPV1 Signaling Pathway

    doi: 10.3390/ijms20184471

    Figure Lengend Snippet: Expression levels of TRPV1-associated signaling pathways in the mice hypothalamus. ( A ) TRPV1, ( B ) pPKA, ( C ) pPI3K, ( D ) pPKCε, ( E ) pERK, ( F ) pp38, ( G ) JNK, ( H ) pAkt, ( I ) pmTOR, ( J ) pNFκB, ( K ) Nav1.7, and ( L ) Nav1.8 expression levels in normal, CFA, CFA + 2 Hz EA, and CFA + sham EA (from left to right). Normal = normal mice; CFA = CFA-induced inflammatory pain; 2 Hz EA = CFA + 2H z EA. Sham EA = CFA + sham EA. * p

    Article Snippet: The following primary antibodies were used: anti-TRPV1 (1:500, Alomone, Israel) and anti-pERK (1:500, Alomone, Israel) from Alomone.

    Techniques: Expressing, Mouse Assay

    Expression levels of TRPV1-associated signaling pathways in the mice periaqueductal gray (PAG). ( A ) TRPV1, ( B ) pPKA, ( C ) pPI3K, ( D ) pPKCε, ( E ) pERK, ( F ) pp38, ( G ) JNK, ( H ) pAkt, ( I ) pmTOR, ( J ) pNFκB, ( K ) Nav1.7, and ( L ) Nav1.8 expression levels in normal, CFA, CFA + 2 Hz EA, and CFA + sham EA (from left to right). Normal = normal mice; CFA = CFA-induced inflammatory pain; 2 Hz EA = CFA + 2 Hz EA. Sham EA = CFA + sham EA. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Distal Electroacupuncture at the LI4 Acupoint Reduces CFA-Induced Inflammatory Pain via the Brain TRPV1 Signaling Pathway

    doi: 10.3390/ijms20184471

    Figure Lengend Snippet: Expression levels of TRPV1-associated signaling pathways in the mice periaqueductal gray (PAG). ( A ) TRPV1, ( B ) pPKA, ( C ) pPI3K, ( D ) pPKCε, ( E ) pERK, ( F ) pp38, ( G ) JNK, ( H ) pAkt, ( I ) pmTOR, ( J ) pNFκB, ( K ) Nav1.7, and ( L ) Nav1.8 expression levels in normal, CFA, CFA + 2 Hz EA, and CFA + sham EA (from left to right). Normal = normal mice; CFA = CFA-induced inflammatory pain; 2 Hz EA = CFA + 2 Hz EA. Sham EA = CFA + sham EA. * p

    Article Snippet: The following primary antibodies were used: anti-TRPV1 (1:500, Alomone, Israel) and anti-pERK (1:500, Alomone, Israel) from Alomone.

    Techniques: Expressing, Mouse Assay