Triton X 100, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Involvement of uracil nucleotides in protection of cardiomyocytes from hypoxic stress"
Article Title: Involvement of uracil nucleotides in protection of cardiomyocytes from hypoxic stress
Journal: Biochemical pharmacology
Figure Legend Snippet: Determination of P2Y receptors in cardiomyocytes by immunofluorescent staining and immunoblotting. Cardiomyocytes were fixed in methanol, permeabilized with 1% Triton X-100, blocked and incubated in the presence of antibodies against P2Y 2 (A) or P2Y 4 (B) receptors. For detection of the bound antibodies, cells were incubated with goat antirabbit FITC (green) following counterstaining with propidium iodide (red). Bars = 10 μm. Proteins isolated from rat cardiomyocyte homogenates were subjected to SDS-PAGE on an 11% acrylamide gel and transferred onto a nitrocellulose membrane. Filters were probed with the indicated P2Y antibodies simultaneously with the neutralizing peptide (np). Protein bands were detected with a secondary antibody coupled to peroxidase by enhanced chemiluminescence (C).
Techniques Used: Staining, Incubation, Isolation, SDS Page, Acrylamide Gel Assay