triton x 100  (Alomone Labs)


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    Alomone Labs triton x 100
    Determination of P2Y receptors in cardiomyocytes by immunofluorescent staining and immunoblotting. Cardiomyocytes were fixed in methanol, permeabilized with 1% Triton X-100, blocked and incubated in the presence of antibodies against P2Y 2 (A) or P2Y 4 (B) receptors. For detection of the bound antibodies, cells were incubated with goat antirabbit FITC (green) following counterstaining with propidium iodide (red). Bars = 10 μm. Proteins isolated from rat cardiomyocyte homogenates were subjected to SDS-PAGE on an 11% acrylamide gel and transferred onto a nitrocellulose membrane. Filters were probed with the indicated P2Y antibodies simultaneously with the neutralizing peptide (np). Protein bands were detected with a secondary antibody coupled to peroxidase by enhanced chemiluminescence (C).
    Triton X 100, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x 100/product/Alomone Labs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    triton x 100 - by Bioz Stars, 2022-09
    94/100 stars

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    1) Product Images from "Involvement of uracil nucleotides in protection of cardiomyocytes from hypoxic stress"

    Article Title: Involvement of uracil nucleotides in protection of cardiomyocytes from hypoxic stress

    Journal: Biochemical pharmacology

    doi: 10.1016/j.bcp.2005.01.018

    Determination of P2Y receptors in cardiomyocytes by immunofluorescent staining and immunoblotting. Cardiomyocytes were fixed in methanol, permeabilized with 1% Triton X-100, blocked and incubated in the presence of antibodies against P2Y 2 (A) or P2Y 4 (B) receptors. For detection of the bound antibodies, cells were incubated with goat antirabbit FITC (green) following counterstaining with propidium iodide (red). Bars = 10 μm. Proteins isolated from rat cardiomyocyte homogenates were subjected to SDS-PAGE on an 11% acrylamide gel and transferred onto a nitrocellulose membrane. Filters were probed with the indicated P2Y antibodies simultaneously with the neutralizing peptide (np). Protein bands were detected with a secondary antibody coupled to peroxidase by enhanced chemiluminescence (C).
    Figure Legend Snippet: Determination of P2Y receptors in cardiomyocytes by immunofluorescent staining and immunoblotting. Cardiomyocytes were fixed in methanol, permeabilized with 1% Triton X-100, blocked and incubated in the presence of antibodies against P2Y 2 (A) or P2Y 4 (B) receptors. For detection of the bound antibodies, cells were incubated with goat antirabbit FITC (green) following counterstaining with propidium iodide (red). Bars = 10 μm. Proteins isolated from rat cardiomyocyte homogenates were subjected to SDS-PAGE on an 11% acrylamide gel and transferred onto a nitrocellulose membrane. Filters were probed with the indicated P2Y antibodies simultaneously with the neutralizing peptide (np). Protein bands were detected with a secondary antibody coupled to peroxidase by enhanced chemiluminescence (C).

    Techniques Used: Staining, Incubation, Isolation, SDS Page, Acrylamide Gel Assay

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    • triton x 100  (Alomone Labs)
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    Alomone Labs triton x 100
    Determination of P2Y receptors in cardiomyocytes by immunofluorescent staining and immunoblotting. Cardiomyocytes were fixed in methanol, permeabilized with 1% Triton X-100, blocked and incubated in the presence of antibodies against P2Y 2 (A) or P2Y 4 (B) receptors. For detection of the bound antibodies, cells were incubated with goat antirabbit FITC (green) following counterstaining with propidium iodide (red). Bars = 10 μm. Proteins isolated from rat cardiomyocyte homogenates were subjected to SDS-PAGE on an 11% acrylamide gel and transferred onto a nitrocellulose membrane. Filters were probed with the indicated P2Y antibodies simultaneously with the neutralizing peptide (np). Protein bands were detected with a secondary antibody coupled to peroxidase by enhanced chemiluminescence (C).
    Triton X 100, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x 100/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triton x 100 - by Bioz Stars, 2022-09
    94/100 stars
    		  Buy from Supplier

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    Determination of P2Y receptors in cardiomyocytes by immunofluorescent staining and immunoblotting. Cardiomyocytes were fixed in methanol, permeabilized with 1% Triton X-100, blocked and incubated in the presence of antibodies against P2Y 2 (A) or P2Y 4 (B) receptors. For detection of the bound antibodies, cells were incubated with goat antirabbit FITC (green) following counterstaining with propidium iodide (red). Bars = 10 μm. Proteins isolated from rat cardiomyocyte homogenates were subjected to SDS-PAGE on an 11% acrylamide gel and transferred onto a nitrocellulose membrane. Filters were probed with the indicated P2Y antibodies simultaneously with the neutralizing peptide (np). Protein bands were detected with a secondary antibody coupled to peroxidase by enhanced chemiluminescence (C).

    Journal: Biochemical pharmacology

    Article Title: Involvement of uracil nucleotides in protection of cardiomyocytes from hypoxic stress

    doi: 10.1016/j.bcp.2005.01.018

    Figure Lengend Snippet: Determination of P2Y receptors in cardiomyocytes by immunofluorescent staining and immunoblotting. Cardiomyocytes were fixed in methanol, permeabilized with 1% Triton X-100, blocked and incubated in the presence of antibodies against P2Y 2 (A) or P2Y 4 (B) receptors. For detection of the bound antibodies, cells were incubated with goat antirabbit FITC (green) following counterstaining with propidium iodide (red). Bars = 10 μm. Proteins isolated from rat cardiomyocyte homogenates were subjected to SDS-PAGE on an 11% acrylamide gel and transferred onto a nitrocellulose membrane. Filters were probed with the indicated P2Y antibodies simultaneously with the neutralizing peptide (np). Protein bands were detected with a secondary antibody coupled to peroxidase by enhanced chemiluminescence (C).

    Article Snippet: Cells were fixed in absolute methanol for 10 min, permeabilized with 0.1% Triton X-100 for 5 min, and blocked in 2% normal goat serum for 10 min. Anti-P2Y2 or anti-P2Y4 antibodies (Alomone Labs) were diluted in PBS to a final concentration of 1:100 and then incubated overnight at room temperature with the cells.

    Techniques: Staining, Incubation, Isolation, SDS Page, Acrylamide Gel Assay