tris hcl  (Thermo Fisher)


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    Name:
    Tris 2 Carboxyethyl phosphine Hydrochloride TCEP
    Description:
    Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by TCEP tris 2 carboxyethyl phosphine Unlike DTT dithiothreitol TCEP does not contain thiols and therefore usually does not need to be removed prior to thiol modification
    Catalog Number:
    t2556
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Cell Analysis|Labeling Thiols|Other Thiol-Reactive Probes|Protein Biology|Protein Labeling|Protein Labeling & Crosslinking|Protein and Antibody Labeling|Protein, Peptide & Antibody Labeling|Labeling Chemistry
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    Structured Review

    Thermo Fisher tris hcl
    Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by TCEP tris 2 carboxyethyl phosphine Unlike DTT dithiothreitol TCEP does not contain thiols and therefore usually does not need to be removed prior to thiol modification
    https://www.bioz.com/result/tris hcl/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris hcl - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    In Vitro:

    Article Title: Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification
    Article Snippet: Mss116p was expressed and purified as previously described (Halls et al. , ) and stored in 10 µl aliquots at −70 °C in storage buffer (20 mm Tris–HCl, pH 7.5, 500 mm KCl, 1 mm EDTA, 1 mm DTT, 50% glycerol) at 5 µm concentration. .. Rpo41p in vitro transcription assay Steady-state transcription reactions were carried out in a volume of 10 µl containing 20 mm Tris–HCl, pH 7.9, 50 mm KCl, 10 mm MgCl2 , 0.1% Tween 20, 5 mm Tris (2-carboxyethyl) phosphine (TCEP; Thermo Fisher Scientific), 7 nm double-stranded DNA template (Figure A), 0.5 mm nucleotide 5′-triphosphates, 0.625 µm (α-32 P-UTP (800 Ci/mm , Perkin-Elmer), 20 nm Rpo41p, 20 nm Mtf1p, and Mss116p as indicated (in 0.8 µl Mss116p storage buffer) at 30 °C for various times, and stopped with an equal volume of transcription stop buffer as described above. .. Labelled RNA products were resolved by electrophoresis on denaturing 15% polyacrylamide denaturing gels for 2 h, as described above.

    Staining:

    Article Title: Dynamics of the ?2-adrenergic G-protein coupled receptor revealed by hydrogen-deuterium exchange
    Article Snippet: HDX was performed on the protein following PNGase F treatment and separation using fast protein liquid chromatography (FPLC) at 4°C. .. β2 AR-460 proteins with and without PNGase F treatment were mixed with NuPAGE LDS sample buffer, and loaded onto 10% Bis-Tris gels and stained with SimplyBue™ SafeStain (Invitrogen), or transferred to a membrane for Western blot using anti-FLAG antibody at 1:1000 (v:v) dilution. ..

    Article Title: The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death
    Article Snippet: Horseradish peroxidase (HRP) anti-mouse IgG (Mouse TrueBlot ULTRA, 18-8817, eBioscience, San Diego, CA, USA) was used to minimize crossreactivity with the heavy and light chain IgG, present in all IP samples. .. In parallel, 20 μ l of each eluate was analyzed by 7% Tris-Acetate SDS-PAGE gels (Life Technologies, Grand Island, NY, USA) and stained afterwards by using EZBlue Gel Staining Reagent (Sigma, St. Louis, MO, USA). ..

    Western Blot:

    Article Title: Dynamics of the ?2-adrenergic G-protein coupled receptor revealed by hydrogen-deuterium exchange
    Article Snippet: HDX was performed on the protein following PNGase F treatment and separation using fast protein liquid chromatography (FPLC) at 4°C. .. β2 AR-460 proteins with and without PNGase F treatment were mixed with NuPAGE LDS sample buffer, and loaded onto 10% Bis-Tris gels and stained with SimplyBue™ SafeStain (Invitrogen), or transferred to a membrane for Western blot using anti-FLAG antibody at 1:1000 (v:v) dilution. ..

    Incubation:

    Article Title: Expression of cadherin 23 isoforms is not conserved: implications for a mouse model of Usher syndrome type 1D
    Article Snippet: A 20 µg protein sample was separated on 4%–20% Tris-glycine gels (Invitrogen) for blots incubated with antibody TF648. .. We used 3%–8% Tris-acetate gels (Invitrogen) for blots incubated with antibody TF7. .. SeeBlue Plus2 pre-stained standard (Invitrogen) was used as size markers.

    SDS Page:

    Article Title: The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death
    Article Snippet: Horseradish peroxidase (HRP) anti-mouse IgG (Mouse TrueBlot ULTRA, 18-8817, eBioscience, San Diego, CA, USA) was used to minimize crossreactivity with the heavy and light chain IgG, present in all IP samples. .. In parallel, 20 μ l of each eluate was analyzed by 7% Tris-Acetate SDS-PAGE gels (Life Technologies, Grand Island, NY, USA) and stained afterwards by using EZBlue Gel Staining Reagent (Sigma, St. Louis, MO, USA). ..

    Article Title: Time- and radiation-dose dependent changes in the plasma proteome after total body irradiation of non-human primates: Implications for biomarker selection
    Article Snippet: High-resolution proteomics The top 20 most abundant proteins (albumin, IgG, transferrin, fibrinogen, IgA, α2-Marcroglobulin, IgM, α1-Antitrypsin, complement C3, haptoglobulin, apolipoprotein A1, A3 and B; α1-Acid Glycoprotein, ceruloplasmin, complement C4, C1q; IgD, prealbumin, and plasminogen) in the plasma were removed using the ProteoPrep 20 Plasma Immunodepletion Kit (Sigma-Aldrich, PROT20). .. Twenty micrograms of protein from depleted plasma was resolved by 4–20% Tris-Glycine SDS-PAGE (Life Technologies) and the proteins were visualized by Coomassie-staining ( ). ..

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  • 97
    Thermo Fisher tris hcl
    Channel current recordings via the spontaneous incorporation of peptides and proteins. ( A ) The gramicidin channel current was recorded with a recording solution containing 150 mM <t>NaCl,</t> 100 nM gramicidin, and 10 mM <t>MOPS-Tris,</t> pH 7.4. The trace was low-pass-filtered at 0.5 kHz. ( B ) The alamethicin channel current was recorded at 100 mV with a solution containing 1 M KCl and 500 nM alamethicin. ( C ) The α-hemolysin current was recorded at 60 mV with 100 mM KCl and 1 μM α-hemolysin, 10 mM Hepes-Tris, pH 7.3. ( D ) A channel current recording of mouse VDAC1. The recording solution contained 1 M KCl and 1 μg/mL VDAC1, 10 mM MOPS-Tris, pH 7.4. The recording was taken at 30 mV.
    Tris Hcl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris hcl/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris hcl - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

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    Channel current recordings via the spontaneous incorporation of peptides and proteins. ( A ) The gramicidin channel current was recorded with a recording solution containing 150 mM NaCl, 100 nM gramicidin, and 10 mM MOPS-Tris, pH 7.4. The trace was low-pass-filtered at 0.5 kHz. ( B ) The alamethicin channel current was recorded at 100 mV with a solution containing 1 M KCl and 500 nM alamethicin. ( C ) The α-hemolysin current was recorded at 60 mV with 100 mM KCl and 1 μM α-hemolysin, 10 mM Hepes-Tris, pH 7.3. ( D ) A channel current recording of mouse VDAC1. The recording solution contained 1 M KCl and 1 μg/mL VDAC1, 10 mM MOPS-Tris, pH 7.4. The recording was taken at 30 mV.

    Journal: Micromachines

    Article Title: A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings

    doi: 10.3390/mi11121070

    Figure Lengend Snippet: Channel current recordings via the spontaneous incorporation of peptides and proteins. ( A ) The gramicidin channel current was recorded with a recording solution containing 150 mM NaCl, 100 nM gramicidin, and 10 mM MOPS-Tris, pH 7.4. The trace was low-pass-filtered at 0.5 kHz. ( B ) The alamethicin channel current was recorded at 100 mV with a solution containing 1 M KCl and 500 nM alamethicin. ( C ) The α-hemolysin current was recorded at 60 mV with 100 mM KCl and 1 μM α-hemolysin, 10 mM Hepes-Tris, pH 7.3. ( D ) A channel current recording of mouse VDAC1. The recording solution contained 1 M KCl and 1 μg/mL VDAC1, 10 mM MOPS-Tris, pH 7.4. The recording was taken at 30 mV.

    Article Snippet: The supernatant was mixed with nickel resin (Thermo Fisher, Waltham, MA, USA) and rotated at 4 °C for 60 min. Nonspecific bound proteins were removed with 5-fold volume of binding buffer, 5-fold volume of wash buffer (6 M GHCl, 300 mM NaCl, 20 mM imidazole, 50 mM Tris-HCl (pH 8.0)), and 5-fold volume of refolding buffer (100 mM NaCl, 0.4% n -dodecyl-N ,N -Dimethylamine-N-oxide (LDAO, Affymetrix), 50 mM Tris-HCl (pH 8.0)).

    Techniques: