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Bio-Rad tris hcl gradient gel
Tris Hcl Gradient Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nucleic Acid Electrophoresis:

Article Title: Differential Kv1.3, KCa3.1, and Kir2.1 expression in “classically” and “alternatively” activated microglia
Article Snippet: For Western blot analysis cells were washed with ice‐cold PBS and incubated with a lysis buffer (150 mM NaCl, 10 mM NaH2 PO4 , 1 mM EDTA, 1% TritonX100, 0.5% SDS) with protease inhibitor cocktail and phosphatase inhibitor (Sigma‐Aldrich). .. Equivalent amounts of protein were analyzed by 4‐15% Tris‐HCl gel electrophoresis (Bio‐Rad, Hercules, CA). ..

SDS Page:

Article Title: Fibroblast Growth Factor 21 (FGF21) Protects against High Fat Diet Induced Inflammation and Islet Hyperplasia in Pancreas
Article Snippet: .. Protein (20 μg) was analyzed by SDS-PAGE on a 4–15% Criterion Tris/HCl gel (Bio-Rad Laboratories) and transferred onto a 45 μM nitrocellulose membrane (Protran; Schleicher & Schuell, Keene, NH). .. Blots were then probed with each specified primary antibody and an HRP-conjugated secondary antibody (Jackson Immunoresearch Laboratories, West Grove, PA).

Article Title: CCN3/CCN2 regulation and the fibrosis of diabetic renal disease
Article Snippet: .. Twenty μl of each prepared sample were then subjected to SDS–PAGE on a 4–15% Tris-HCL gradient gel (Bio-Rad) and transferred to a PVDF membrane (Millipore, Bedford, MA). .. The membrane was blocked with 5% nonfat dried milk in TBS + 0.1% Tween 20 for 1 h at room temperature and then incubated with a specific polyclonal anti-CCN3 antibody (K19 at 1:1,000 dilution) produced by us, against a 19 amino acid sequence in C-terminal module.

Electrophoresis:

Article Title: Moderate Alcohol Exposure during the Rat Equivalent to the Third Trimester of Human Pregnancy Alters Regulation of GABAA Receptor-Mediated Synaptic Transmission by Dopamine in the Basolateral Amygdala
Article Snippet: Control experiments demonstrated that this concentration of protein was within the linear dynamic range for the western blot assay (not shown). .. Electrophoresis was performed in 4–15% Tris–HCl precast gels (BioRad) at 140 V for 60 min at 4°C. ..

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  • 96
    Bio-Rad tris hcl gradient gel
    Western blot analysis of nuclear extracts from macrophages of pIpC-treated PPARγ-MXCre + mice and similarly treated PPARγ-MXCre − mice. A total of 10 μg of protein from nuclear extracts of macrophages and 10 μg of total protein from Hepa-1 cells transfected with an expression vector for PPARγ1 (pSG5-PPARγ cDNA) were subjected to electrophoresis on a 4 to 15% <t>Tris-HCl</t> gradient gel (Bio-Rad), transferred to <t>Immobilon-P</t> membranes (Millipore), and probed as recommended by the manufacturer with anti-PPARγ antibodies (Santa Cruz Biotechnologies) specific for the N terminus (E-8, PPARγ antibody) (A) and the C terminus (H-100, PPARγ antibody) (B) of the PPARγ protein. Detection of immunoreactive proteins was done by using an enhanced chemiluminescence blot detection system (Amersham).
    Tris Hcl Gradient Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris hcl gradient gel/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris hcl gradient gel - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

    97
    Bio-Rad gradient sds polyacrylamide tris hcl gels
    2-DE separation of proteins extracted from leaves of healthy of Las-infected lemon plants. ( A ) Representative leaf and gel containing extracted proteins separated via 2-DE of a healthy lemon plant. ( B ) Representative leaf and gel containing extracted proteins separated via 2-DE of a Las-infected lemon plant. Two-year old healthy plants were either graft-inoculated with side shoots from PCR-confirmed Las-infected bud sticks or uninoculated and leaf samples were analyzed at six months post-inoculation. A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient <t>SDS-polyacrylamide</t> <t>Tris-HCl</t> gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r , relative molecular mass; pI, isoelectric point.
    Gradient Sds Polyacrylamide Tris Hcl Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gradient sds polyacrylamide tris hcl gels/product/Bio-Rad
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gradient sds polyacrylamide tris hcl gels - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    96
    Bio-Rad sds page tris hcl criterion gradients gels
    Tuberin was phosphorylated at threonine 1462 via the Akt–PTEN pathway in the frontal cortex of AD and PD/DLB patients. Total cell lysates were subjected to <t>SDS-PAGE</t> and then immunoblotted with antibodies to p-tuberin, tuberin, p-Akt, Akt, p-PTEN,
    Sds Page Tris Hcl Criterion Gradients Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sds page tris hcl criterion gradients gels/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sds page tris hcl criterion gradients gels - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

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    Western blot analysis of nuclear extracts from macrophages of pIpC-treated PPARγ-MXCre + mice and similarly treated PPARγ-MXCre − mice. A total of 10 μg of protein from nuclear extracts of macrophages and 10 μg of total protein from Hepa-1 cells transfected with an expression vector for PPARγ1 (pSG5-PPARγ cDNA) were subjected to electrophoresis on a 4 to 15% Tris-HCl gradient gel (Bio-Rad), transferred to Immobilon-P membranes (Millipore), and probed as recommended by the manufacturer with anti-PPARγ antibodies (Santa Cruz Biotechnologies) specific for the N terminus (E-8, PPARγ antibody) (A) and the C terminus (H-100, PPARγ antibody) (B) of the PPARγ protein. Detection of immunoreactive proteins was done by using an enhanced chemiluminescence blot detection system (Amersham).

    Journal: Molecular and Cellular Biology

    Article Title: Conditional Disruption of the Peroxisome Proliferator-Activated Receptor ? Gene in Mice Results in Lowered Expression of ABCA1, ABCG1, and apoE in Macrophages and Reduced Cholesterol Efflux

    doi: 10.1128/MCB.22.8.2607-2619.2002

    Figure Lengend Snippet: Western blot analysis of nuclear extracts from macrophages of pIpC-treated PPARγ-MXCre + mice and similarly treated PPARγ-MXCre − mice. A total of 10 μg of protein from nuclear extracts of macrophages and 10 μg of total protein from Hepa-1 cells transfected with an expression vector for PPARγ1 (pSG5-PPARγ cDNA) were subjected to electrophoresis on a 4 to 15% Tris-HCl gradient gel (Bio-Rad), transferred to Immobilon-P membranes (Millipore), and probed as recommended by the manufacturer with anti-PPARγ antibodies (Santa Cruz Biotechnologies) specific for the N terminus (E-8, PPARγ antibody) (A) and the C terminus (H-100, PPARγ antibody) (B) of the PPARγ protein. Detection of immunoreactive proteins was done by using an enhanced chemiluminescence blot detection system (Amersham).

    Article Snippet: Then, 10 μg of total protein from Hepa-1 cells and 10 μg of protein from nuclear extracts of macrophages were subjected to electrophoresis on a 4 to 15% Tris-HCl gradient gel (Bio-Rad, Hercules, Calif.), transferred to Immobilon-P membranes (Millipore, Bedford, Mass.), and probed according to the manufacturer's recommendations with anti-PPARγ antibodies (E-8 and H-100; Santa Cruz Biotechnology, Inc., Santa Cruz, Calif.) as indicated.

    Techniques: Western Blot, Mouse Assay, Transfection, Expressing, Plasmid Preparation, Electrophoresis

    2-DE separation of proteins extracted from leaves of healthy of Las-infected lemon plants. ( A ) Representative leaf and gel containing extracted proteins separated via 2-DE of a healthy lemon plant. ( B ) Representative leaf and gel containing extracted proteins separated via 2-DE of a Las-infected lemon plant. Two-year old healthy plants were either graft-inoculated with side shoots from PCR-confirmed Las-infected bud sticks or uninoculated and leaf samples were analyzed at six months post-inoculation. A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient SDS-polyacrylamide Tris-HCl gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r , relative molecular mass; pI, isoelectric point.

    Journal: PLoS ONE

    Article Title: Study on Citrus Response to Huanglongbing Highlights a Down-Regulation of Defense-Related Proteins in Lemon Plants Upon 'Ca. Liberibacter asiaticus' Infection

    doi: 10.1371/journal.pone.0067442

    Figure Lengend Snippet: 2-DE separation of proteins extracted from leaves of healthy of Las-infected lemon plants. ( A ) Representative leaf and gel containing extracted proteins separated via 2-DE of a healthy lemon plant. ( B ) Representative leaf and gel containing extracted proteins separated via 2-DE of a Las-infected lemon plant. Two-year old healthy plants were either graft-inoculated with side shoots from PCR-confirmed Las-infected bud sticks or uninoculated and leaf samples were analyzed at six months post-inoculation. A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient SDS-polyacrylamide Tris-HCl gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r , relative molecular mass; pI, isoelectric point.

    Article Snippet: Second dimension electrophoresis was performed in 8-16% gradient SDS-polyacrylamide Tris-HCl gels (Criterion precast gels, Bio-Rad) in a twelve-gel cell system (Criterion Dodeca Cell, Bio-Rad).

    Techniques: Infection, Polymerase Chain Reaction, Stripping Membranes, Staining

    PDQuest-generated master gel image showing the general spot pattern of matched protein spots from the total leaf proteome of healthy or Las-infected lemon plants. Labeled spots were differentially produced in response to Las-infection and described in Table 1 . A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient SDS-polyacrylamide Tris-HCl gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r , relative molecular mass; pI, isoelectric point.

    Journal: PLoS ONE

    Article Title: Study on Citrus Response to Huanglongbing Highlights a Down-Regulation of Defense-Related Proteins in Lemon Plants Upon 'Ca. Liberibacter asiaticus' Infection

    doi: 10.1371/journal.pone.0067442

    Figure Lengend Snippet: PDQuest-generated master gel image showing the general spot pattern of matched protein spots from the total leaf proteome of healthy or Las-infected lemon plants. Labeled spots were differentially produced in response to Las-infection and described in Table 1 . A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient SDS-polyacrylamide Tris-HCl gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r , relative molecular mass; pI, isoelectric point.

    Article Snippet: Second dimension electrophoresis was performed in 8-16% gradient SDS-polyacrylamide Tris-HCl gels (Criterion precast gels, Bio-Rad) in a twelve-gel cell system (Criterion Dodeca Cell, Bio-Rad).

    Techniques: Generated, Infection, Labeling, Produced, Stripping Membranes, Staining

    Differentially produced protein spots from 2-DE analysis of total leaf proteins from healthy or Las-infected lemon plants. Panels A-M show magnified views of protein spots in representative 2-DE gels containing separated total proteins from leaves of healthy or Las-infected lemon plants. Labeled spots showed significant changes and correspond to the spots presented in in Figure 2 and Tables 2 and 3 . Two-year old healthy plants were either graft-inoculated with side shoots from PCR-confirmed Las-infected bud sticks or uninoculated and leaf samples were analyzed at six months post-inoculation. A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient SDS-polyacrylamide Tris-HCl gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r , relative molecular mass; pI, isoelectric point.

    Journal: PLoS ONE

    Article Title: Study on Citrus Response to Huanglongbing Highlights a Down-Regulation of Defense-Related Proteins in Lemon Plants Upon 'Ca. Liberibacter asiaticus' Infection

    doi: 10.1371/journal.pone.0067442

    Figure Lengend Snippet: Differentially produced protein spots from 2-DE analysis of total leaf proteins from healthy or Las-infected lemon plants. Panels A-M show magnified views of protein spots in representative 2-DE gels containing separated total proteins from leaves of healthy or Las-infected lemon plants. Labeled spots showed significant changes and correspond to the spots presented in in Figure 2 and Tables 2 and 3 . Two-year old healthy plants were either graft-inoculated with side shoots from PCR-confirmed Las-infected bud sticks or uninoculated and leaf samples were analyzed at six months post-inoculation. A sum of 200 µg of total protein was separated according to charge on a pH 4-7 IpG strip and according to mass on 8-16% gradient SDS-polyacrylamide Tris-HCl gels. Protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M r , relative molecular mass; pI, isoelectric point.

    Article Snippet: Second dimension electrophoresis was performed in 8-16% gradient SDS-polyacrylamide Tris-HCl gels (Criterion precast gels, Bio-Rad) in a twelve-gel cell system (Criterion Dodeca Cell, Bio-Rad).

    Techniques: Produced, Infection, Labeling, Polymerase Chain Reaction, Stripping Membranes, Staining

    Experimental strategy for comparing peptide- vs protein-level enrichments. (A) Experimental design applying on-resin iTRAQ-labeling. (B) Silver-staining image of SDS–PAGE of enriched Cys-peptides. For each lane of the 4–20% Tris–HCl precast gel, 5 of 30 μl of DTT-eluted Cys-peptides was loaded. The higher protein bands in protein-level enrichment samples indicate incomplete on-resin digestion. (C) An MS/MS spectrum of the Cys-peptide GVLECQVSR from obscurin protein and its zoom-in spectrum of reporter-ion region showing the SNO abundance increased in response to GSNO treatment.

    Journal: Free radical biology & medicine

    Article Title: Quantitative site-specific reactivity profiling of S-nitrosylation in mouse skeletal muscle using cysteinyl peptide enrichment coupled with mass spectrometry

    doi: 10.1016/j.freeradbiomed.2012.12.010

    Figure Lengend Snippet: Experimental strategy for comparing peptide- vs protein-level enrichments. (A) Experimental design applying on-resin iTRAQ-labeling. (B) Silver-staining image of SDS–PAGE of enriched Cys-peptides. For each lane of the 4–20% Tris–HCl precast gel, 5 of 30 μl of DTT-eluted Cys-peptides was loaded. The higher protein bands in protein-level enrichment samples indicate incomplete on-resin digestion. (C) An MS/MS spectrum of the Cys-peptide GVLECQVSR from obscurin protein and its zoom-in spectrum of reporter-ion region showing the SNO abundance increased in response to GSNO treatment.

    Article Snippet: SDS–PAGE was carried out with a 4–20% precast linear gradient Tris–HCl polyacrylamide gel (Bio-Rad) to assess enrichment efficiency and total SNO levels.

    Techniques: Labeling, Silver Staining, SDS Page, Mass Spectrometry

    Tuberin was phosphorylated at threonine 1462 via the Akt–PTEN pathway in the frontal cortex of AD and PD/DLB patients. Total cell lysates were subjected to SDS-PAGE and then immunoblotted with antibodies to p-tuberin, tuberin, p-Akt, Akt, p-PTEN,

    Journal:

    Article Title: Role of Tuberin in Neuronal Degeneration

    doi: 10.1007/s11064-007-9558-8

    Figure Lengend Snippet: Tuberin was phosphorylated at threonine 1462 via the Akt–PTEN pathway in the frontal cortex of AD and PD/DLB patients. Total cell lysates were subjected to SDS-PAGE and then immunoblotted with antibodies to p-tuberin, tuberin, p-Akt, Akt, p-PTEN,

    Article Snippet: A total of 60 μg of protein from each sample was loaded on 4–20% SDS-PAGE Tris–HCl Criterion gradients gels (Bio-Rad) and samples were analyzed by western blot with antibodies as indicated in the figures.

    Techniques: SDS Page