tris hcl buffer (Millipore)

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Magnesium chloride

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m4880

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Millipore tris hcl buffer
Magnesium chloride

https://www.bioz.com/result/tris hcl buffer/product/Millipore
Average 97 stars, based on 1 article reviews
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tris hcl buffer - by Bioz Stars, 2021-03

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1) Product Images from "Influence of Conformation of M. tuberculosis RNase P Protein Subunit on Its Function"

Article Title: Influence of Conformation of M. tuberculosis RNase P Protein Subunit on Its Function

Journal: PLoS ONE

doi: 10.1371/journal.pone.0153798

Activity of protein preparations on pre-tRNA at low ammonium acetate. The pre-tRNA processing activity with different amounts of RNase P protein preparations alone, in the absence of RNA component, was assayed in 50 mM Tris-HCl (pH 7.4), 10 mM magnesium chloride and 100 mM ammonium acetate. S denotes the substrate alone reaction.

Figure Legend Snippet: Activity of protein preparations on pre-tRNA at low ammonium acetate. The pre-tRNA processing activity with different amounts of RNase P protein preparations alone, in the absence of RNA component, was assayed in 50 mM Tris-HCl (pH 7.4), 10 mM magnesium chloride and 100 mM ammonium acetate. S denotes the substrate alone reaction.

Techniques Used: Activity Assay

other:

Article Title: In vitro evaluation of the toxic effects of MgO nanostructure in Hela cell line
Article Snippet: Chemicals and Synthesis of MgO Nanotubes Magnesium chloride (MgCl2 ), sodium hydroxide (NaOH), polyvinyl alcohol (PVA), and ethanol were purchased from Sigma Aldrich and used as chemical reagents.

Incubation:

Article Title: Inducible dissociation of SCFMet30 ubiquitin ligase mediates a rapid transcriptional response to cadmium
Article Snippet: Recombinant His6-HA Met4 produced in insect cells was affinity purified on nickel resin and treated with lambda phosphatase. .. Immobilized SCFMet30 was washed three times with 1 ml of ubiquitination buffer (50 mM Tris–HCl pH 7.5, 10 mM MgCl2 , 2 mM ATP, 50 μM DTT), incubated with His6 Uba1 (E1), His6 Cdc34 (E2), ubiquitin (Sigma) or methylated ubiquitin (Affiniti) and Met4 in 10 μl ubiquitylation buffer at 30°C for 1 h. Products were separated by SDS–PAGE and immunoblotted with anti-HA (12CA5) antibody. ..

Article Title: Antitumour potential of BPT: a dual inhibitor of cdk4 and tubulin polymerization
Article Snippet: .. The plates were further incubated for 30 min, the cell monolayers were washed two times with sterile PBS at room temperature and then 100 μl tubulin extraction buffer (1 mM MgCl2 , 2 mM EGTA, 0.5% NP40 and 20 mM Tris-HCl (pH 6.8)) supplemented with 2 mM phenylmethylsulfonyl fluoride, and a protease inhibitor cocktail (Sigma-Aldrich; cat. no. P8340) was added per well. .. After a short and vigorous vortex mixing, the cell lysates were incubated at room temperature for 5 min and then centrifuged at 16 000 r.p.m. for 10 min to separate the soluble and polymerized tubulin fractions.

Methylation:

SDS Page:

Recombinant:

Article Title: Targeting of Dicer-2 and RNA by a Viral RNA Silencing Suppressor in Drosophila Cells
Article Snippet: RNase III-mediated cleavage assays were conducted according to our standard protocol , using T7-transcribed 500-bp dsRNA derived from GFP as precursor. .. Reactions were performed in 20-μl reaction mixture volumes containing 0.1 μM dsRNA, 1× RNase III buffer (20 mM Tris-HCl, 0.5 mM EDTA, 5 mM MgCl2 , 1 mM DTT [Sigma, SaintLouis, MO], 140 mM NaCl, 2.7 mM KCl [pH 7.9]), and 1 U recombinant RNase III (a generous gift from Yi Zhang). .. After incubation at 37°C for 30 min, reaction products were resolved by Tris-borate-EDTA (TBE)–agarose gel electrophoresis on 1.2% gels, and the RNA was stained with ethidium bromide at 1 μg/ml.

Activity Assay:

Article Title: Photosynthetic Membranes of Synechocystis or Plants Convert Sunlight to Photocurrent through Different Pathways due to Different Architectures
Article Snippet: .. Oxygen evolution activity of the preparations was determined using a Clark-type electrode (Hansatech Instruments, UK) with 10 μg chl of the isolated thylakoid preparations in buffer B (50mM MES/NaOH pH = 6.0, 15mM NaCl, 5mM MgCl, 2mM CaCl2 ) and 0.3 mM 2,6-dichlorobenzoquinone (DCBQ, Sigma) as the exogenous electron acceptor. ..

Isolation:

Protease Inhibitor:

Crystallization Assay:

Article Title: Structural basis of Mycobacterium tuberculosistranscription and transcription inhibition
Article Snippet: For Mtb RPitc2, the complex for crystallization was prepared by mixing 16 μl 50 μM Mtb RNAP holoenzyme (in 20 mM Tris-HCl, pH 8.0, 75 mM NaCl, 5 mM dithiothreitol, 5 mM MgCl2 ), 4 μl 0.4 mM of nucleic-acid scaffold RPo (in 5 mM Tris-HCl, pH 7.7, 0.2 M NaCl, and 10 mM MgCl2 ), and 1 μl 25 mM GpA (in water), and incubating 1 h at 25°C. .. For Mtb RPo, RPitc3, and RPitc4 in complex with Rif, complexes for crystallization were prepared by incubating 16 μl 50 μM Mtb RNAP σA holoenzyme (in 20 mM Tris-HCl, pH 8.0, 75 mM NaCl, 5 mM MgCl2 , and 5 mM dithiothreitol,) and 0.5 μl 8 mM Rif (Sigma-Aldrich, Inc.), 0.5 h at 25°C, and then adding 4 μl 0.4 mM of nucleic-acid scaffold RPo, RPitc3, or RPitc4 (in 5 mM Tris-HCl, pH 7.7, 0.2 M NaCl, and 10 mM MgCl2 ), and incubating 1 h at 25°C. .. For Mtb RPitc,2 in complex with Rif, the complex for crystallization was prepared by incubating 16 μl 50 μM Mtb RNAP σA holoenzyme (in 20 mM Tris-HCl, pH 8.0, 75 mM NaCl, 5 mM MgCl2 , and 5 mM dithiothreitol) and 0.5 μl 8 mM Rif, 0.5 h at 25°C, and then adding 4 μl 0.4 mM of nucleic-acid scaffold RPo (in 5 mM Tris-HCl, pH 7.7, 0.2 M NaCl, and 10 mM MgCl2 ) and 1 μl 25 mM GpA (in water) and incubating 1 h at 25°C.

Immunoprecipitation:

Article Title: OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3
Article Snippet: For chain cleavage assays, DUBs (30 ng μl−1 ) were incubated in DUB buffer (50 mM Tris–HCl pH 7.5, 5 mM DTT, 100 mM NaCl) with the indicated ubiquitin chains (0.13 μg μl−1 , K48 ub2–7 , K63 ub2–7 (Boston Biochem), K48 ub2 (Axel Knebel)) on an IP shaker for 1 h at 30 °C. .. Ubiquitylation assays of SMAD2 or FLAG-SMAD2/3/4 (immunoprecipitated from HEK293 cells treated with 50 pM TGFβ for 1 h before lysis) were performed in ubiquitylation assay buffer (50 mM Tris–HCl pH 7.5, 5 mM MgCl2, 2 μM ATP) with His6 -UBE1 (0.1 μM), UBE2D1 (1 μM or indicated concentrations), His6 -NEDD4L (1 μM) and ubiquitin (50 μM; Sigma) for 1 h at 30 °C on an IP shaker. ..