tris edta  (Millipore)

 
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    Name:
    Tris EDTA buffer solution
    Description:
    TE buffer is the most commonly used buffer solution in molecular biology TE is a component of Tris a common pH buffer and EDTA molecule that chelate cations TE Tris EDTA Buffer solubilizes DNA and RNA while protecting it from degradation TE Buffer is used in nucleic acid isolation which may be done prior to Northern or Southern blot hybridization
    Catalog Number:
    T9285
    Price:
    None
    Applications:
    Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.. Tris-EDTA buffer solution has been used in culture medium.. Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release β-lactamases from bacterial cell.. It has been used for the suspension of bacterial spores.. It has also been used as a buffer in immunohistochemistry.
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    Structured Review

    Millipore tris edta
    Tris EDTA buffer solution
    TE buffer is the most commonly used buffer solution in molecular biology TE is a component of Tris a common pH buffer and EDTA molecule that chelate cations TE Tris EDTA Buffer solubilizes DNA and RNA while protecting it from degradation TE Buffer is used in nucleic acid isolation which may be done prior to Northern or Southern blot hybridization
    https://www.bioz.com/result/tris edta/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris edta - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples"

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    Journal: Sensors (Basel, Switzerland)

    doi: 10.3390/s150510088

    ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.
    Figure Legend Snippet: ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Techniques Used: Concentration Assay

    ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.
    Figure Legend Snippet: ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Techniques Used: Raman Spectroscopy

    2) Product Images from "Long-chain carboxychromanols, metabolites of vitamin E, are potent inhibitors of cyclooxygenases"

    Article Title: Long-chain carboxychromanols, metabolites of vitamin E, are potent inhibitors of cyclooxygenases

    Journal:

    doi: 10.1073/pnas.0810962106

    13′-COOH is a competitive inhibitor of the COX-1 cyclooxygenase activity. 13′-COOH (square, 0 μM; triangle, 7.5 μM; circle, 15 μM; diamond, 25 μM) and COX-1 were mixed in 0.1 M Tris (pH 8.0) with 5 mM EDTA,
    Figure Legend Snippet: 13′-COOH is a competitive inhibitor of the COX-1 cyclooxygenase activity. 13′-COOH (square, 0 μM; triangle, 7.5 μM; circle, 15 μM; diamond, 25 μM) and COX-1 were mixed in 0.1 M Tris (pH 8.0) with 5 mM EDTA,

    Techniques Used: Activity Assay

    Related Articles

    other:

    Article Title: RUNX1-mediated alphaherpesvirus-host trans-species chromatin interaction promotes viral transcription
    Article Snippet: The beads were washed with RIPA-LS and tris-EDTA (TE) buffer.

    Protease Inhibitor:

    Article Title: Impairment of a cyanobacterial glycosyltransferase that modifies a pilin results in biofilm development
    Article Snippet: .. The supernatant was removed and passed through 0.22 mm filter, desiccated, and resuspended in 1.1 ml TE buffer supplemented with a protease inhibitor cocktail (Sigma, P8465-5ML). ..

    Article Title: The Effect of a Novel Serine Protease Inhibitor on Inflammation and Intestinal Permeability in a Murine Colitis Transfer Model
    Article Snippet: .. The samples were stored on ice in a Tris-EDTA buffer [PBS containing 10 mM Tris, 1 mM EDTA, 0.5% v/v Tween20 containing a protease-inhibitor cocktail (Sigma-Aldrich)] at a ratio of 100 mg tissue per mL buffer. ..

    Incubation:

    Article Title: Targeting therapy-resistant lung cancer stem cells via disruption of the AKT/TSPYL5/PTEN positive-feedback loop
    Article Snippet: .. Following cell fixation, cells were incubated with anti-TSPYL5 antibody in a permeabilization solution of PBS containing 0.1% Triton X-100 and 1% bovine serum albumin at 4 °C overnight. ..

    In Utero:

    Article Title: Ire1α-Regulated Rate of mRNA Translation is Required for Acquisition of Identity and Polarity in Upper Layer Cortical Neurons
    Article Snippet: .. In utero electroporation (IUE)Prior to IUE, DNA was diluted in TE buffer and mixed with 0.1% Fast Green FCF (Sigma- Aldrich). ..

    Electroporation:

    Article Title: Ire1α-Regulated Rate of mRNA Translation is Required for Acquisition of Identity and Polarity in Upper Layer Cortical Neurons
    Article Snippet: .. In utero electroporation (IUE)Prior to IUE, DNA was diluted in TE buffer and mixed with 0.1% Fast Green FCF (Sigma- Aldrich). ..

    Sonication:

    Article Title: Identification of PAX6 and NFAT4 as the transcriptional regulators of lncRNA Mrhl in neuronal progenitors
    Article Snippet: .. For monitoring sonication efficiency and DNA quantification, 10 μl aliquots was kept aside, diluted in 10 μl of TE buffer and treated with 10 μg of RNaseA (Sigma, R6513) for 30 minutes at 37°C followed by 20 μg of proteinase K (Thermo Fisher Scientific, EO0491) for 1 hour at 55 °C. ..

    Blocking Assay:

    Article Title: Reduced neutralizing activity of post-SARS-CoV-2 vaccination serum against variants B.1.617.2, B.1.351, B.1.1.7+E484K and a sub-variant of C.37
    Article Snippet: .. Permeabilization solution was removed, plates were washed with 200 μl/well of PBS twice and 200 μl/well of blocking solution consisting of PBS 3% milk (American Bio) were added for 1 h at RT. ..

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  • 99
    Millipore tris edta
    ( a ) Concentration-dependent SERS spectra using AgNPs and <t>Tris-EDTA</t> in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.
    Tris Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris edta/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris edta - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    95
    Millipore 10x concentration
    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, <t>10X</t> Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value
    10x Concentration, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x concentration/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x concentration - by Bioz Stars, 2021-09
    95/100 stars
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    98
    Millipore tbe buffer
    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× <t>TBE</t> buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At <t>PCNA2;</t> lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after
    Tbe Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbe buffer/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tbe buffer - by Bioz Stars, 2021-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    doi: 10.3390/s150510088

    Figure Lengend Snippet: ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Article Snippet: Chemicals Cr(III) and the other ionic substances of NaCl, KNO3 , Mg(NO3 )2 , Ca(NO3 )2 , Cr(NO3 )3 , Mn(NO3 )2 , FeC2 O4 , Fe(NO3 )3 , Co(NO3 )2 , Ni(NO3 )2 , Cu(NO3 )2 , Zn(NO3 )2 , NH4 NO3 , Cd(NO3 )2 , Hg(NO3 )2 , Pb(NO3 )2 , and K2 Cr2 O7 (or Na2 CrO4 ) along with silver nitrate, sodium citrate, Tris-EDTA (pH 8.0), and EDTA acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Concentration Assay

    ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    doi: 10.3390/s150510088

    Figure Lengend Snippet: ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Article Snippet: Chemicals Cr(III) and the other ionic substances of NaCl, KNO3 , Mg(NO3 )2 , Ca(NO3 )2 , Cr(NO3 )3 , Mn(NO3 )2 , FeC2 O4 , Fe(NO3 )3 , Co(NO3 )2 , Ni(NO3 )2 , Cu(NO3 )2 , Zn(NO3 )2 , NH4 NO3 , Cd(NO3 )2 , Hg(NO3 )2 , Pb(NO3 )2 , and K2 Cr2 O7 (or Na2 CrO4 ) along with silver nitrate, sodium citrate, Tris-EDTA (pH 8.0), and EDTA acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Raman Spectroscopy

    Schematic diagram of human papillomavirus (hrHPV) assay workflow. MCA, melting curve analysis; PCR, polymerase chain reaction; TE, Tris-EDTA.

    Journal: Journal of Global Oncology

    Article Title: Implementation of Multicolor Melt Curve Analysis for High-Risk Human Papilloma Virus Detection in Low- and Middle-Income Countries: A Pilot Study for Expanded Cervical Cancer Screening in Honduras

    doi: 10.1200/JGO.17.00035

    Figure Lengend Snippet: Schematic diagram of human papillomavirus (hrHPV) assay workflow. MCA, melting curve analysis; PCR, polymerase chain reaction; TE, Tris-EDTA.

    Article Snippet: hrHPV Detection A crude lysate from swab A was obtained by submerging the flocked tip of the cytobrush into a clean microcentrifuge tube containing 200 μL of 1× Tris-EDTA buffer solution (pH 8.0; Sigma-Aldrich, St. Louis, MO) and boiled ( > 95°C) for 8 minutes.

    Techniques: Polymerase Chain Reaction

    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Journal: Frontiers in Oncology

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity

    doi: 10.3389/fonc.2020.01784

    Figure Lengend Snippet: C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Article Snippet: Then 40 h later, cells were treated simultaneously with 60 μM H2 O2 and Ctrl-C2C12 or P3F-C2C12 exosomes at 10X concentration.

    Techniques: Derivative Assay, Western Blot, Software, Zymography, Activity Assay, Cell Culture, Quantitation Assay

    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment

    doi: 10.1002/pro.117

    Figure Lengend Snippet: Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Article Snippet: His At PCNA1, At PCNA1, His At PCNA2, At PCNA2, and the heterotrimer were separated by 4% native PAGE in 1× TBE buffer and electrotransferred onto a PVDF membrane (0.2 μm) (Millipore), as described previously.

    Techniques: Purification, Recombinant, Clear Native PAGE