tris edta  (Millipore)

 
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    Name:
    Tris EDTA buffer solution
    Description:
    TE buffer is the most commonly used buffer solution in molecular biology TE is a component of Tris a common pH buffer and EDTA molecule that chelate cations TE Tris EDTA Buffer solubilizes DNA and RNA while protecting it from degradation TE Buffer is used in nucleic acid isolation which may be done prior to Northern or Southern blot hybridization
    Catalog Number:
    T9285
    Price:
    None
    Applications:
    Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.. Tris-EDTA buffer solution has been used in culture medium.. Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release β-lactamases from bacterial cell.. It has been used for the suspension of bacterial spores.. It has also been used as a buffer in immunohistochemistry.
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    Structured Review

    Millipore tris edta
    Tris EDTA buffer solution
    TE buffer is the most commonly used buffer solution in molecular biology TE is a component of Tris a common pH buffer and EDTA molecule that chelate cations TE Tris EDTA Buffer solubilizes DNA and RNA while protecting it from degradation TE Buffer is used in nucleic acid isolation which may be done prior to Northern or Southern blot hybridization
    https://www.bioz.com/result/tris edta/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris edta - by Bioz Stars, 2021-05
    99/100 stars

    Images

    1) Product Images from "Long-chain carboxychromanols, metabolites of vitamin E, are potent inhibitors of cyclooxygenases"

    Article Title: Long-chain carboxychromanols, metabolites of vitamin E, are potent inhibitors of cyclooxygenases

    Journal:

    doi: 10.1073/pnas.0810962106

    13′-COOH is a competitive inhibitor of the COX-1 cyclooxygenase activity. 13′-COOH (square, 0 μM; triangle, 7.5 μM; circle, 15 μM; diamond, 25 μM) and COX-1 were mixed in 0.1 M Tris (pH 8.0) with 5 mM EDTA,
    Figure Legend Snippet: 13′-COOH is a competitive inhibitor of the COX-1 cyclooxygenase activity. 13′-COOH (square, 0 μM; triangle, 7.5 μM; circle, 15 μM; diamond, 25 μM) and COX-1 were mixed in 0.1 M Tris (pH 8.0) with 5 mM EDTA,

    Techniques Used: Activity Assay

    2) Product Images from "Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples"

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    Journal: Sensors (Basel, Switzerland)

    doi: 10.3390/s150510088

    ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.
    Figure Legend Snippet: ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Techniques Used: Concentration Assay

    ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.
    Figure Legend Snippet: ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Techniques Used: Raman Spectroscopy

    Related Articles

    Fluorescence:

    Article Title: Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes
    Article Snippet: FACS analysis For cell cycle analysis, 106 cells were fixed using the FoxP3 Staining Kit (00-5523-00 eBioscience) for 30 min at room temperature in the dark. .. Samples were then resuspended in permeabilization buffer containing 20 μg of RNase A (R6513 Sigma) and 1 μg of propidium iodide (PI) (130-093-233 Miltenyi Biotech) for 30 min. PI fluorescence was analyzed using a BD Accuri C6 cell analyzer with BD CSampler Analysis Software. .. Results were analyzed with FlowJo software version 10 (TreeStar).

    Software:

    Article Title: Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes
    Article Snippet: FACS analysis For cell cycle analysis, 106 cells were fixed using the FoxP3 Staining Kit (00-5523-00 eBioscience) for 30 min at room temperature in the dark. .. Samples were then resuspended in permeabilization buffer containing 20 μg of RNase A (R6513 Sigma) and 1 μg of propidium iodide (PI) (130-093-233 Miltenyi Biotech) for 30 min. PI fluorescence was analyzed using a BD Accuri C6 cell analyzer with BD CSampler Analysis Software. .. Results were analyzed with FlowJo software version 10 (TreeStar).

    Incubation:

    Article Title: In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160
    Article Snippet: .. In some experiments, cells were incubated in permeabilization buffer containing either 5 mM ATP (Sigma-Aldrich), GTP (Sigma-Aldrich), AMP-PNP (Roche), or ADP (Calbiochem) for at least 5 min before FRAP measurements. ..

    Concentration Assay:

    Article Title: Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner
    Article Snippet: .. The cells were washed for 3 × 2 min with PBS, followed by a 2-min wash with permeabilization buffer, followed by a 5-min permeabilization with digitonin (Sigma–Aldrich) at a concentration of 50 μg/ml supplemented with an energy regenerating system of 100 µM ATP (Roche), 100 μM GTP (Roche), 4 mM creatine phosphate (Roche), and 20 U/ml creatine kinase (Roche) in permeabilization buffer. ..

    Protease Inhibitor:

    Article Title: Phenylarsine Oxide Binding Reveals Redox-Active and Potential Regulatory Vicinal Thiols on the Catalytic Subunit of Protein Phosphatase 2A
    Article Snippet: Whole brains from 7–8 week-old Sprague–Dawley rats were obtained on dry ice from Pel-Freez Biologicals (Rogers, AZ) and were stored until use at −80°C. .. For each S100 fraction prepared, one whole brain was partially thawed and homogenized in a glass-Teflon homogenizer in 15 mL of Tris–EDTA buffer (50 mM Tris, 1 mM EDTA, 1 mM benzamidine, pH 7.4) to which was added 50 μL of mammalian protease inhibitor cocktail (Sigma Chemical) per brain. ..

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  • 99
    Millipore tris edta
    ( a ) Concentration-dependent SERS spectra using AgNPs and <t>Tris-EDTA</t> in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.
    Tris Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris edta/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris edta - by Bioz Stars, 2021-05
    99/100 stars
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    95
    Millipore 10x concentration
    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, <t>10X</t> Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value
    10x Concentration, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x concentration/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x concentration - by Bioz Stars, 2021-05
    95/100 stars
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    98
    Millipore tbe buffer
    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× <t>TBE</t> buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At <t>PCNA2;</t> lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after
    Tbe Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbe buffer/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tbe buffer - by Bioz Stars, 2021-05
    98/100 stars
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    Image Search Results


    ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    doi: 10.3390/s150510088

    Figure Lengend Snippet: ( a ) Concentration-dependent SERS spectra using AgNPs and Tris-EDTA in the range of 0.005–10 μM of Cr(III); ( b ) A magnified view of the vibrational bands between 200 and 750 cm −1 in the Cr(III) concentration-dependent SERS spectra; ( c ) A calibration curve of the vibrational band intensities at ~563 cm −1 with respect to those at ~918 cm −1 . The inset shows a linear fitting result for the 0.05 and 1.0 μM regions.

    Article Snippet: Chemicals Cr(III) and the other ionic substances of NaCl, KNO3 , Mg(NO3 )2 , Ca(NO3 )2 , Cr(NO3 )3 , Mn(NO3 )2 , FeC2 O4 , Fe(NO3 )3 , Co(NO3 )2 , Ni(NO3 )2 , Cu(NO3 )2 , Zn(NO3 )2 , NH4 NO3 , Cd(NO3 )2 , Hg(NO3 )2 , Pb(NO3 )2 , and K2 Cr2 O7 (or Na2 CrO4 ) along with silver nitrate, sodium citrate, Tris-EDTA (pH 8.0), and EDTA acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Concentration Assay

    ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Silver Nanoparticle-Enhanced Resonance Raman Sensor of Chromium(III) in Seawater Samples

    doi: 10.3390/s150510088

    Figure Lengend Snippet: ( a ) Surface-enhanced Raman spectroscopy (SERS) spectra of detection of Cr(III) using Tris(hydroxymethyl)aminomethane (Tris)-EDTA on AgNPs. Selective tests were performed for 50 μM of Cr 3+ , K + , Cd 2+ , Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Na + , Cu 2+ , NH 4 + , Hg 2+ , Ni 2+ , Fe 3+ , Pb 2+ , Fe 2+ , and Zn 2+ ions; ( b ) Stick diagram of the intensities at ~563 cm −1 with respect to those at ~918 cm −1 versus 16 ionic species; ( c ) SERS spectra of the mixture of the 15 ions to test the competitive reactions; ( d ) Comparative SERS spectra of EDTA and EDTA-Cr 3+ on AgNPs at pH 4.0 and pH 8.0.

    Article Snippet: Chemicals Cr(III) and the other ionic substances of NaCl, KNO3 , Mg(NO3 )2 , Ca(NO3 )2 , Cr(NO3 )3 , Mn(NO3 )2 , FeC2 O4 , Fe(NO3 )3 , Co(NO3 )2 , Ni(NO3 )2 , Cu(NO3 )2 , Zn(NO3 )2 , NH4 NO3 , Cd(NO3 )2 , Hg(NO3 )2 , Pb(NO3 )2 , and K2 Cr2 O7 (or Na2 CrO4 ) along with silver nitrate, sodium citrate, Tris-EDTA (pH 8.0), and EDTA acetic acid were purchased from Sigma Aldrich (St. Louis, MO, USA).

    Techniques: Raman Spectroscopy

    Schematic diagram of human papillomavirus (hrHPV) assay workflow. MCA, melting curve analysis; PCR, polymerase chain reaction; TE, Tris-EDTA.

    Journal: Journal of Global Oncology

    Article Title: Implementation of Multicolor Melt Curve Analysis for High-Risk Human Papilloma Virus Detection in Low- and Middle-Income Countries: A Pilot Study for Expanded Cervical Cancer Screening in Honduras

    doi: 10.1200/JGO.17.00035

    Figure Lengend Snippet: Schematic diagram of human papillomavirus (hrHPV) assay workflow. MCA, melting curve analysis; PCR, polymerase chain reaction; TE, Tris-EDTA.

    Article Snippet: hrHPV Detection A crude lysate from swab A was obtained by submerging the flocked tip of the cytobrush into a clean microcentrifuge tube containing 200 μL of 1× Tris-EDTA buffer solution (pH 8.0; Sigma-Aldrich, St. Louis, MO) and boiled ( > 95°C) for 8 minutes.

    Techniques: Polymerase Chain Reaction

    C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Journal: Frontiers in Oncology

    Article Title: Effects of the Oncoprotein PAX3-FOXO1 on Modulation of Exosomes Function and Protein Content: Implications on Oxidative Stress Protection and Enhanced Plasticity

    doi: 10.3389/fonc.2020.01784

    Figure Lengend Snippet: C2C12-P3F-derived exosomes promote angiogenesis and induce stemness of recipient cells. (A) Western blot for the indicated proteins in MEFs treated with Exo-free (EF) media, 10X Ctrl-C2C12-derived exosomes, and 10X P3F-C2C12-derived exosomes. GAPDH is used as a loading control. Histograms presenting the levels of phosphorylation of ERK and 4EBP1 compared to Exo-free condition, quantified by ImageJ software from n = 3 different western blots. (B) Zymography image of digested regions showing MMP2 activity in MEFs treated with either Ctrl-C2C12-derived exosomes or P3F-C2C12-derived exosomes for 72 h. Relative MMP-2 activity from 3 independent experiments was quantified by ImageJ software and presented by a histogram. (C) Representative phase contrast photomicrographs of endothelial tube formation of HUVECs cultured on Matrigel with either Exo-Free medium (EF), 1X, or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. Quantitation of the total number of nodes, total length and total mesh area of the different conditions are shown in the histogram, which represent the mean of 3 independent experiments, each performed in triplicate. (D,E) Representative phase contrast photomicrographs and histograms showing the ratio of the spheres formed by p53−/− MEFs (D) or C2C12 cells (E) treated with either Exo-Free medium (EF) or 10X C2C12-MSCV-derived exosomes or C2C12-P3F-derived exosomes, as specified. All data are reported as means of at least three independent experiments ± SD. Asterisks (*) denote a statistically significant difference ( p -value

    Article Snippet: Then 40 h later, cells were treated simultaneously with 60 μM H2 O2 and Ctrl-C2C12 or P3F-C2C12 exosomes at 10X concentration.

    Techniques: Derivative Assay, Western Blot, Software, Zymography, Activity Assay, Cell Culture, Quantitation Assay

    Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment

    doi: 10.1002/pro.117

    Figure Lengend Snippet: Analysis of heterotrimerization. Purified recombinant proteins were separated by 4% native PAGE in 1× TBE buffer at 4°C. Lane 1: His At PCNA1; lane 2: At PCNA1; lane 3: His At PCNA2; lane 4: At PCNA2; lane 5: His At PCNA1 and At PCNA2 mixed after

    Article Snippet: His At PCNA1, At PCNA1, His At PCNA2, At PCNA2, and the heterotrimer were separated by 4% native PAGE in 1× TBE buffer and electrotransferred onto a PVDF membrane (0.2 μm) (Millipore), as described previously.

    Techniques: Purification, Recombinant, Clear Native PAGE