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Bio-Rad tris buffered saline tbs
Tris Buffered Saline Tbs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tris buffered saline tbs/product/Bio-Rad
Average 99 stars, based on 134 article reviews
Price from $9.99 to $1999.99
tris buffered saline tbs - by Bioz Stars, 2020-11
99/100 stars

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Related Articles

Electrophoresis:

Article Title: Annexin A2 is a Robo4 ligand that modulates ARF6 activation-associated cerebral trans-endothelial permeability
Article Snippet: .. After electrophoresis and blotting onto polyvinylidene difluoride membranes, the membranes were blocked in Tris-buffered saline containing 5% nonfat milk for 60 min at room temperature. .. The membranes were then probed with primary antibodies against ZO-1 (96594, Abcam), VE-cadherin (33169, Abcam), Occludin (64482, Abcam), Claudin-5 (15106, Abcam), and Na+ K+ATPase (198366, Abcam) at 4℃ overnight.

Incubation:

Article Title: MicroRNA-Based Prophylaxis in a Mouse Model of Cirrhosis and Liver Cancer
Article Snippet: .. Rabbit antibodies against mTOR (7C10, 2983; Cell Signaling Technology, Danvers, MA, USA), PAK4 (3242; Cell Signaling Technology), PTEN (9552; Cell Signaling Technology), and p27 Kip1 (2552; Cell Signaling Technology) were diluted in 5% w/v BSA (A4503, Sigma-Aldrich), 1× Tris-buffered saline (TBS), and 0.1% Tween20 (Bio-Rad) and incubated at 4°C for 16 hr. .. An anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody (clone 2D9, TA802519; OriGene) was used as a housekeeper.

Article Title: The evolutionarily conserved C-terminal peptide of troponin I is an independently configured regulatory structure to function as a myofilament Ca2+-desensitizer
Article Snippet: .. The membranes were then blocked with 1% bovine serum albumin (BSA) in Tris-buffered saline (TBS) at room temperature for 30 minutes, and incubated with mAb TnI-1 against an epitope in the C-terminal end segment of TnI [ ] or mAb 3C11 against the Tx3 tag [ ] at 4°C overnight. .. Washed with 0.05% Triton X-100 and 0.1% SDS in TBS, the membranes were further incubated with alkaline phosphatase-conjugated goat anti-mouse IgG secondary antibody, washed again, and developed in BCIP-NTB substrate solution as described previously [ ].

Article Title: Cyclocarya paliurus ethanol leaf extracts protect against diabetic cardiomyopathy in db/db mice via regulating PI3K/Akt/NF-κB signaling
Article Snippet: .. The resultant blots were blocked for 2 h with 5% non-fat milk in tris-buffered saline with Tween 20 (TBST), followed by overnight incubation at 4°C with appropriate primary antibodies. .. Blots were then washed thrice with TBST prior to staining with appropriate secondary antibodies for 2 h. A GelDox XR System (Bio-Rad, CA, USA) and the Quantity One software were used for protein band detection.

other:

Article Title: Hypersalinity drives physiological and morphological changes in Limia perugiae (Poeciliidae)
Article Snippet: The protein was then transferred to a polyvinylidene fluoride (PVDF) membrane, blocked with casein (#161-0782; Bio-Rad, Hercules, CA USA), and probed with Complex I, ATP synthase, and Na+ /K+ ATPase antibodies for two hours at room temperature.

Article Title: NAD(P)H oxidase modulates angiogenesis and the development of portosystemic collaterals and splanchnic hyperaemia in portal hypertensive rats
Article Snippet: Membranes were blocked in Tris‐buffered saline (TBS) with 0.05% Tween 20 (TBS‐T buffer) containing 10% (wt/vol) non‐fat dry milk.

Article Title: A Polyphenol-Rich Extract From Muscadine Grapes Inhibits Triple-Negative Breast Tumor Growth
Article Snippet: Primary antibodies were diluted in 5% bovine serum albumin or 5% Blotting-Grade Blocker in Tris-buffered saline with 0.1% Tween and applied to membranes overnight at 4°C with gentle agitation.

Blocking Assay:

Article Title: Uncoupling Protein-2 Up-regulation and Enhanced Cyanide Toxicity are Mediated by PPARα Activation and Oxidative Stress
Article Snippet: .. After blocking with Tris-buffered saline containing 5% nonfat dry milk and 0.1% Tween 20, the membrane was exposed to primary UCP-2 antibody or β-actin antibody for 3 h at room temperature on a shaker. .. The UCP-2 antibody was a rabbit anti-mouse polyclonal antibody (1:2000) directed toward the C-terminal domain of UCP-2 (Alpha Diagnostic International, Inc., San Antonio, TX).

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  • 92
    Bio-Rad rabbit anti cd31
    Representative and quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment and MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). ( A – F , S ) The number of <t>CD31-positive</t> endothelial cells significantly increased in the sEH KO before and after MCAO and post-MCAO AUDA-treated mice, while ( G – L ) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO and AUDA-treated mice (arrows) ( T ). The percentage of NeuN- and p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. ( M – R , U – V ) GFAP-positive astrogliosis and Iba1-positive microglial infiltration decreased in post-MCAO sEH KO and AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. * p
    Rabbit Anti Cd31, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad clarity western ecl substrate
    Results of analysis using the KinomeView kit upon treatment of IGR-N-91 with the compounds ST013881 and ST022328 identifies alterations in the PKA, PKC, ATM/ATR, AMPK, Akt, and MAPK/CDK signaling pathways. Immunoblotting was performed using 30 µg of protein lysates from the IGR-N-91 cell line treated with the ST013881 and ST022328 compounds for 2, 4, 6, and 12 h. Membranes were blocked and primary antibodies were diluted 1:1000 in <t>TBS-T.</t> Upon horseradish peroxidase (HRP)-coupled secondary antibody incubation, membranes were developed using the Clarity Western <t>ECL</t> Substrate (Bio-Rad). PKA Protein kinase A, PKC protein kinase C, ATM/ATR ataxia-telangiectasia mutated/ATM- and Rad3-related, AMPK 5′ AMP-activated protein kinase, Akt protein kinase B, MAPK/CDK mitogen-activated protein kinase/cyclin-dependent kinase
    Clarity Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2096 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clarity western ecl substrate/product/Bio-Rad
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    85
    Bio-Rad sds brain lysates
    Immunoblot analysis of Aβ species from sequentially extracted human prefrontal cortical tissue lysates . Human hippocampi from Alzheimer's disease (AD), pathological aging (PA) and normal (N) cohorts were sequentially extracted with <t>TBS,</t> 2% <t>SDS</t> extracted lysates from human subjects. Representative immunoblots probed with 82E1 antibody are shown. Control lanes includes cell lysates expressing C99 (CTFβ) and recombinant Aβ1-42 (43 pmol). Aβ, amyloid β; SDS, sodium dodecyl sulfate; TBS, Tris buffered saline.
    Sds Brain Lysates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bio-Rad m tb h37rv
    Effect of altered expression of Rv1211 on growth kinetics of M. tb in vitro . Cultures were induced with 0.2% acetamide and growth was monitored by measuring A 600nm (A) and by CFU assay (B). Graph depicts growth kinetics of M. tb <t>H37Rv</t> (WT) , M. tuberculosis-pJFR19 (EV), M.tb-Rv1211S and M.tb-Rv1211AS for period of 21 days. Data was considered significant (*) if p
    M Tb H37rv, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative and quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment and MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). ( A – F , S ) The number of CD31-positive endothelial cells significantly increased in the sEH KO before and after MCAO and post-MCAO AUDA-treated mice, while ( G – L ) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO and AUDA-treated mice (arrows) ( T ). The percentage of NeuN- and p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. ( M – R , U – V ) GFAP-positive astrogliosis and Iba1-positive microglial infiltration decreased in post-MCAO sEH KO and AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. * p

    Journal: Scientific Reports

    Article Title: Blockade of soluble epoxide hydrolase attenuates post-ischemic neuronal hyperexcitation and confers resilience against stroke with TrkB activation

    doi: 10.1038/s41598-017-18558-6

    Figure Lengend Snippet: Representative and quantitative immunohistochemistry of the peri-infarct cortex before the bregma (coordinate + 0.58) in normal WT, normal sEH KO, MCAO WT, MCAO sEH KO, MCAO with vehicle treatment and MCAO with AUDA treatment mice (n = 2 for normal groups, n = 5–6 for all groups subjected to MCAO). ( A – F , S ) The number of CD31-positive endothelial cells significantly increased in the sEH KO before and after MCAO and post-MCAO AUDA-treated mice, while ( G – L ) Note the increased phosphorylated TrkB (p-TrkB) immunoreactivity that partially co-localized with NeuN-positive neurons, particularly in the sEH KO and AUDA-treated mice (arrows) ( T ). The percentage of NeuN- and p-TrkB-positive double labeling in the neuronal population was significantly increased compared to that in the corresponding controls. ( M – R , U – V ) GFAP-positive astrogliosis and Iba1-positive microglial infiltration decreased in post-MCAO sEH KO and AUDA treatment groups relative to that in the corresponding controls. The scale bar represents 50 µm. * p

    Article Snippet: The slides were incubated in a blocking solution containing 3% donkey serum albumin (Abcam) and 0.3% Triton X-100 (Sigma-Aldrich) in TBS for 1 hour at room temperature and immunostained with primary antibodies including rabbit anti-CD31 (1:200, BioRad, MCA23886A), anti-p-TrkB (1:200, Abcam, ab109684), anti-NeuN (1:200, Abcam, ab104224), anti-GFAP (1:500, Abcam, ab7260), and anti-Iba1 (1:200, Novus, NB100-1028) overnight at 4 °C.

    Techniques: Immunohistochemistry, Mouse Assay, Labeling

    Results of analysis using the KinomeView kit upon treatment of IGR-N-91 with the compounds ST013881 and ST022328 identifies alterations in the PKA, PKC, ATM/ATR, AMPK, Akt, and MAPK/CDK signaling pathways. Immunoblotting was performed using 30 µg of protein lysates from the IGR-N-91 cell line treated with the ST013881 and ST022328 compounds for 2, 4, 6, and 12 h. Membranes were blocked and primary antibodies were diluted 1:1000 in TBS-T. Upon horseradish peroxidase (HRP)-coupled secondary antibody incubation, membranes were developed using the Clarity Western ECL Substrate (Bio-Rad). PKA Protein kinase A, PKC protein kinase C, ATM/ATR ataxia-telangiectasia mutated/ATM- and Rad3-related, AMPK 5′ AMP-activated protein kinase, Akt protein kinase B, MAPK/CDK mitogen-activated protein kinase/cyclin-dependent kinase

    Journal: Oncology and Therapy

    Article Title: Identification of Novel Small-Molecule Kinase Modulators for the Treatment of Neuroblastoma

    doi: 10.1007/s40487-020-00113-5

    Figure Lengend Snippet: Results of analysis using the KinomeView kit upon treatment of IGR-N-91 with the compounds ST013881 and ST022328 identifies alterations in the PKA, PKC, ATM/ATR, AMPK, Akt, and MAPK/CDK signaling pathways. Immunoblotting was performed using 30 µg of protein lysates from the IGR-N-91 cell line treated with the ST013881 and ST022328 compounds for 2, 4, 6, and 12 h. Membranes were blocked and primary antibodies were diluted 1:1000 in TBS-T. Upon horseradish peroxidase (HRP)-coupled secondary antibody incubation, membranes were developed using the Clarity Western ECL Substrate (Bio-Rad). PKA Protein kinase A, PKC protein kinase C, ATM/ATR ataxia-telangiectasia mutated/ATM- and Rad3-related, AMPK 5′ AMP-activated protein kinase, Akt protein kinase B, MAPK/CDK mitogen-activated protein kinase/cyclin-dependent kinase

    Article Snippet: After washing with TBS-T, the membranes were developed using Clarity Western ECL Substrate (Bio-Rad) on a Fusion Fx imaging system (Vilber Lourmat Sté, Collégien, France).

    Techniques: Incubation, Western Blot

    Immunoblot analysis of Aβ species from sequentially extracted human prefrontal cortical tissue lysates . Human hippocampi from Alzheimer's disease (AD), pathological aging (PA) and normal (N) cohorts were sequentially extracted with TBS, 2% SDS extracted lysates from human subjects. Representative immunoblots probed with 82E1 antibody are shown. Control lanes includes cell lysates expressing C99 (CTFβ) and recombinant Aβ1-42 (43 pmol). Aβ, amyloid β; SDS, sodium dodecyl sulfate; TBS, Tris buffered saline.

    Journal: Alzheimer's Research & Therapy

    Article Title: Overlapping profiles of A? peptides in the Alzheimer's disease and pathological aging brains

    doi: 10.1186/alzrt121

    Figure Lengend Snippet: Immunoblot analysis of Aβ species from sequentially extracted human prefrontal cortical tissue lysates . Human hippocampi from Alzheimer's disease (AD), pathological aging (PA) and normal (N) cohorts were sequentially extracted with TBS, 2% SDS extracted lysates from human subjects. Representative immunoblots probed with 82E1 antibody are shown. Control lanes includes cell lysates expressing C99 (CTFβ) and recombinant Aβ1-42 (43 pmol). Aβ, amyloid β; SDS, sodium dodecyl sulfate; TBS, Tris buffered saline.

    Article Snippet: Western blotting TBS, RIPA and 2% SDS brain lysates, heated at 50°C for three minutes in the presence of denaturing sample buffer, were separated on 4% to 12% Bis-Tris gel (Bio-Rad) in 1X 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Bio-Rad).

    Techniques: Western Blot, Expressing, Recombinant

    Biochemical analysis of Aβ from human brain lysates . Human prefrontal cortical tissue from Alzheimer's disease (AD), pathological aging (PA) and normal controls (NDC) was sequentially extracted with TBS ( A ), RIPA ( B ), 2% SDS ( C ) and 70% FA ( D ). End-specific sandwich ELISAs measuring Aβ1-40, Aβ1-42, Aβtotal and Aβx-42 are presented for each of these fractions. N = 16 (AD), 8 (PA) and 6 (NDC). (*** P

    Journal: Alzheimer's Research & Therapy

    Article Title: Overlapping profiles of A? peptides in the Alzheimer's disease and pathological aging brains

    doi: 10.1186/alzrt121

    Figure Lengend Snippet: Biochemical analysis of Aβ from human brain lysates . Human prefrontal cortical tissue from Alzheimer's disease (AD), pathological aging (PA) and normal controls (NDC) was sequentially extracted with TBS ( A ), RIPA ( B ), 2% SDS ( C ) and 70% FA ( D ). End-specific sandwich ELISAs measuring Aβ1-40, Aβ1-42, Aβtotal and Aβx-42 are presented for each of these fractions. N = 16 (AD), 8 (PA) and 6 (NDC). (*** P

    Article Snippet: Western blotting TBS, RIPA and 2% SDS brain lysates, heated at 50°C for three minutes in the presence of denaturing sample buffer, were separated on 4% to 12% Bis-Tris gel (Bio-Rad) in 1X 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Bio-Rad).

    Techniques:

    Effect of altered expression of Rv1211 on growth kinetics of M. tb in vitro . Cultures were induced with 0.2% acetamide and growth was monitored by measuring A 600nm (A) and by CFU assay (B). Graph depicts growth kinetics of M. tb H37Rv (WT) , M. tuberculosis-pJFR19 (EV), M.tb-Rv1211S and M.tb-Rv1211AS for period of 21 days. Data was considered significant (*) if p

    Journal: Scientific Reports

    Article Title: Calmodulin-like protein from M. tuberculosis H37Rv is required during infection

    doi: 10.1038/srep06861

    Figure Lengend Snippet: Effect of altered expression of Rv1211 on growth kinetics of M. tb in vitro . Cultures were induced with 0.2% acetamide and growth was monitored by measuring A 600nm (A) and by CFU assay (B). Graph depicts growth kinetics of M. tb H37Rv (WT) , M. tuberculosis-pJFR19 (EV), M.tb-Rv1211S and M.tb-Rv1211AS for period of 21 days. Data was considered significant (*) if p

    Article Snippet: The recombinant constructs were introduced into M. tb H37Rv via electroporation by using a cell porator (Bio-Rad) to obtain strains expressing the antisense and sense mRNA of Rv1211.

    Techniques: Expressing, In Vitro, Colony-forming Unit Assay, IF-P

    Expression level of Rv1211 in M. tuberculosis Rv1211AS. Expression levels of Rv1211 was determined in wild type M. tb H37Rv and uninduced (UI) and acetamide induced (I) M. tuberculosis Rv1211AS by qRT PCR. Graph depicts fold change in expression of Rv1211 in M. tb Rv1211AS (induced and uninduced) relative to Rv1211 expression in wild type M. tb H37Rv. Values represent mean ± SD of duplicates from two independent experiments.

    Journal: Scientific Reports

    Article Title: Calmodulin-like protein from M. tuberculosis H37Rv is required during infection

    doi: 10.1038/srep06861

    Figure Lengend Snippet: Expression level of Rv1211 in M. tuberculosis Rv1211AS. Expression levels of Rv1211 was determined in wild type M. tb H37Rv and uninduced (UI) and acetamide induced (I) M. tuberculosis Rv1211AS by qRT PCR. Graph depicts fold change in expression of Rv1211 in M. tb Rv1211AS (induced and uninduced) relative to Rv1211 expression in wild type M. tb H37Rv. Values represent mean ± SD of duplicates from two independent experiments.

    Article Snippet: The recombinant constructs were introduced into M. tb H37Rv via electroporation by using a cell porator (Bio-Rad) to obtain strains expressing the antisense and sense mRNA of Rv1211.

    Techniques: Expressing, Quantitative RT-PCR

    Effect of altered expression of Rv1211 on growth of M. tb ex vivo . THP-1 cells were infected with induced cultures of M. tb H37Rv (WT) , M. tb-pJFR19 (EV), M.tb-Rv1211S and M.tb-Rv1211AS separately. Intracellular growth and survival was monitored by enumerating CFUs as given in the materials and methods. Graph depicts intracellular growth kinetics of M. tb H37Rv (WT) , M. tbs-pJFR19 (EV), M.tb-Rv1211S and M.tb-Rv1211AS over a period of 7 days post infection. Data was considered significant (*) if p

    Journal: Scientific Reports

    Article Title: Calmodulin-like protein from M. tuberculosis H37Rv is required during infection

    doi: 10.1038/srep06861

    Figure Lengend Snippet: Effect of altered expression of Rv1211 on growth of M. tb ex vivo . THP-1 cells were infected with induced cultures of M. tb H37Rv (WT) , M. tb-pJFR19 (EV), M.tb-Rv1211S and M.tb-Rv1211AS separately. Intracellular growth and survival was monitored by enumerating CFUs as given in the materials and methods. Graph depicts intracellular growth kinetics of M. tb H37Rv (WT) , M. tbs-pJFR19 (EV), M.tb-Rv1211S and M.tb-Rv1211AS over a period of 7 days post infection. Data was considered significant (*) if p

    Article Snippet: The recombinant constructs were introduced into M. tb H37Rv via electroporation by using a cell porator (Bio-Rad) to obtain strains expressing the antisense and sense mRNA of Rv1211.

    Techniques: Expressing, Ex Vivo, Infection, IF-P

    Effect of reduced expression of Rv1211 on growth of M. tb ex vivo . MDMs were infected with both M. tb H37Rv and M. tb-Rv1211AS separately. Intracellular growth and survival was monitored by enumerating CFUs as given in the materials and methods. Graph depicts intracellular growth kinetics of M. tb H37Rv and M. tb-Rv1211AS over a period of 3 days post infection. Data was considered significant (*) if p

    Journal: Scientific Reports

    Article Title: Calmodulin-like protein from M. tuberculosis H37Rv is required during infection

    doi: 10.1038/srep06861

    Figure Lengend Snippet: Effect of reduced expression of Rv1211 on growth of M. tb ex vivo . MDMs were infected with both M. tb H37Rv and M. tb-Rv1211AS separately. Intracellular growth and survival was monitored by enumerating CFUs as given in the materials and methods. Graph depicts intracellular growth kinetics of M. tb H37Rv and M. tb-Rv1211AS over a period of 3 days post infection. Data was considered significant (*) if p

    Article Snippet: The recombinant constructs were introduced into M. tb H37Rv via electroporation by using a cell porator (Bio-Rad) to obtain strains expressing the antisense and sense mRNA of Rv1211.

    Techniques: Expressing, Ex Vivo, Infection, IF-P