tris acetate ethylenediaminetetraacetic acid edta buffer  (Fisher Scientific)

 
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    Fisher Scientific tris acetate ethylenediaminetetraacetic acid edta buffer
    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM <t>bis-tris</t> HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.
    Tris Acetate Ethylenediaminetetraacetic Acid Edta Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris acetate ethylenediaminetetraacetic acid edta buffer/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris acetate ethylenediaminetetraacetic acid edta buffer - by Bioz Stars, 2021-05
    86/100 stars

    Images

    1) Product Images from "Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami"

    Article Title: Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19092513

    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.
    Figure Legend Snippet: AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Techniques Used: Incubation

    2) Product Images from "Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami"

    Article Title: Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19092513

    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.
    Figure Legend Snippet: AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Techniques Used: Incubation

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
    Article Snippet: Gel electrophoresis for PCR analysis PCR product was determined through gel electrophoresis and fluorescent imaging of gel. .. PCR products were applied into 2% low melting agarose gel (catalog number E-3126-25; ISC BioExpress, Kaysville, UT, USA), using a Fisher Scientific (catalog number FB200) power supply at 80 V and 1 A for 60 min in a 1x tris-acetate-EDTA (TAE) buffer solution (catalog number BP13324; Fischer Scientific). ..

    Article Title: Inhibition of Hippocampal Synaptic Activity by ATP, Hypoxia or Oxygen-Glucose Deprivation Does Not Require CD73
    Article Snippet: The ‘slowdown PCR’ program was used with cDNA because it had been found previously to reduce primer-dimers and give cleaner results. .. A 1% agarose gel, containing ethidium bromide for visualization, was run in Tris acetate-EDTA (TAE) buffer (40 mM Tris acetate and 1 mM EDTA) for 30 −90 min at 105 V and the PCR products were cut from the gel while being illuminated by a FBTI-88 Transilluminator (Fisher Scientific). .. Quantitative PCR for TNAP For quantitative PCR reaction mixtures, with a final volume of 50 µl, consisted of 1 µl reverse transcribed cDNA, 0.5 µM primers, 2.5 mM MgCl2 , 0.2 mM dNTPs, 0.1× SYBR Green I, 0.25 U Platinum Taq polymerase and10 nM fluorescent calibration dye.

    Article Title: ACP5 (Uteroferrin): Phylogeny of an Ancient and Conserved Gene Expressed in the Endometrium of Mammals 1
    Article Snippet: .. PCR amplification consisted of 40 cycles at 94°C for 15 sec for denaturation, 55°C for 30 sec for annealing, and 72°C for 1 min for extension, followed by a final extension cycle at 72°C for 5 min. Amplicons were electrophoresed on 3% (w/v) agarose gel containing 1 μg/ml of ethidium bromide in Tris acetate-ethylenediaminetetra-acetic acid (EDTA) buffer (40 mM Tris-acetate and 2 mM EDTA), visualized on an ultraviolet transluminator (Fisher Scientific), and photographed with a Canon G-7 power shot digital camera (Canon, Inc.) using the Digi-Doc-IT imaging system (UVP, LLC). .. For further sequencing, purification of amplicons was achieved with the QIAquick Extraction Kit (Qiagen, Inc.) following the manufacturer's guidelines.

    Agarose Gel Electrophoresis:

    Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
    Article Snippet: Gel electrophoresis for PCR analysis PCR product was determined through gel electrophoresis and fluorescent imaging of gel. .. PCR products were applied into 2% low melting agarose gel (catalog number E-3126-25; ISC BioExpress, Kaysville, UT, USA), using a Fisher Scientific (catalog number FB200) power supply at 80 V and 1 A for 60 min in a 1x tris-acetate-EDTA (TAE) buffer solution (catalog number BP13324; Fischer Scientific). ..

    Article Title: Inhibition of Hippocampal Synaptic Activity by ATP, Hypoxia or Oxygen-Glucose Deprivation Does Not Require CD73
    Article Snippet: The ‘slowdown PCR’ program was used with cDNA because it had been found previously to reduce primer-dimers and give cleaner results. .. A 1% agarose gel, containing ethidium bromide for visualization, was run in Tris acetate-EDTA (TAE) buffer (40 mM Tris acetate and 1 mM EDTA) for 30 −90 min at 105 V and the PCR products were cut from the gel while being illuminated by a FBTI-88 Transilluminator (Fisher Scientific). .. Quantitative PCR for TNAP For quantitative PCR reaction mixtures, with a final volume of 50 µl, consisted of 1 µl reverse transcribed cDNA, 0.5 µM primers, 2.5 mM MgCl2 , 0.2 mM dNTPs, 0.1× SYBR Green I, 0.25 U Platinum Taq polymerase and10 nM fluorescent calibration dye.

    Article Title: ACP5 (Uteroferrin): Phylogeny of an Ancient and Conserved Gene Expressed in the Endometrium of Mammals 1
    Article Snippet: .. PCR amplification consisted of 40 cycles at 94°C for 15 sec for denaturation, 55°C for 30 sec for annealing, and 72°C for 1 min for extension, followed by a final extension cycle at 72°C for 5 min. Amplicons were electrophoresed on 3% (w/v) agarose gel containing 1 μg/ml of ethidium bromide in Tris acetate-ethylenediaminetetra-acetic acid (EDTA) buffer (40 mM Tris-acetate and 2 mM EDTA), visualized on an ultraviolet transluminator (Fisher Scientific), and photographed with a Canon G-7 power shot digital camera (Canon, Inc.) using the Digi-Doc-IT imaging system (UVP, LLC). .. For further sequencing, purification of amplicons was achieved with the QIAquick Extraction Kit (Qiagen, Inc.) following the manufacturer's guidelines.

    Amplification:

    Article Title: ACP5 (Uteroferrin): Phylogeny of an Ancient and Conserved Gene Expressed in the Endometrium of Mammals 1
    Article Snippet: .. PCR amplification consisted of 40 cycles at 94°C for 15 sec for denaturation, 55°C for 30 sec for annealing, and 72°C for 1 min for extension, followed by a final extension cycle at 72°C for 5 min. Amplicons were electrophoresed on 3% (w/v) agarose gel containing 1 μg/ml of ethidium bromide in Tris acetate-ethylenediaminetetra-acetic acid (EDTA) buffer (40 mM Tris-acetate and 2 mM EDTA), visualized on an ultraviolet transluminator (Fisher Scientific), and photographed with a Canon G-7 power shot digital camera (Canon, Inc.) using the Digi-Doc-IT imaging system (UVP, LLC). .. For further sequencing, purification of amplicons was achieved with the QIAquick Extraction Kit (Qiagen, Inc.) following the manufacturer's guidelines.

    Size-exclusion Chromatography:

    Article Title: ACP5 (Uteroferrin): Phylogeny of an Ancient and Conserved Gene Expressed in the Endometrium of Mammals 1
    Article Snippet: .. PCR amplification consisted of 40 cycles at 94°C for 15 sec for denaturation, 55°C for 30 sec for annealing, and 72°C for 1 min for extension, followed by a final extension cycle at 72°C for 5 min. Amplicons were electrophoresed on 3% (w/v) agarose gel containing 1 μg/ml of ethidium bromide in Tris acetate-ethylenediaminetetra-acetic acid (EDTA) buffer (40 mM Tris-acetate and 2 mM EDTA), visualized on an ultraviolet transluminator (Fisher Scientific), and photographed with a Canon G-7 power shot digital camera (Canon, Inc.) using the Digi-Doc-IT imaging system (UVP, LLC). .. For further sequencing, purification of amplicons was achieved with the QIAquick Extraction Kit (Qiagen, Inc.) following the manufacturer's guidelines.

    Imaging:

    Article Title: ACP5 (Uteroferrin): Phylogeny of an Ancient and Conserved Gene Expressed in the Endometrium of Mammals 1
    Article Snippet: .. PCR amplification consisted of 40 cycles at 94°C for 15 sec for denaturation, 55°C for 30 sec for annealing, and 72°C for 1 min for extension, followed by a final extension cycle at 72°C for 5 min. Amplicons were electrophoresed on 3% (w/v) agarose gel containing 1 μg/ml of ethidium bromide in Tris acetate-ethylenediaminetetra-acetic acid (EDTA) buffer (40 mM Tris-acetate and 2 mM EDTA), visualized on an ultraviolet transluminator (Fisher Scientific), and photographed with a Canon G-7 power shot digital camera (Canon, Inc.) using the Digi-Doc-IT imaging system (UVP, LLC). .. For further sequencing, purification of amplicons was achieved with the QIAquick Extraction Kit (Qiagen, Inc.) following the manufacturer's guidelines.

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    Fisher Scientific tris acetate ethylenediaminetetraacetic acid edta buffer
    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM <t>bis-tris</t> HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.
    Tris Acetate Ethylenediaminetetraacetic Acid Edta Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris acetate ethylenediaminetetraacetic acid edta buffer/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris acetate ethylenediaminetetraacetic acid edta buffer - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Fisher Scientific tris hydroxymethyl aminomethane acetate edta buffer
    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM <t>bis-tris</t> HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.
    Tris Hydroxymethyl Aminomethane Acetate Edta Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris hydroxymethyl aminomethane acetate edta buffer/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris hydroxymethyl aminomethane acetate edta buffer - by Bioz Stars, 2021-05
    86/100 stars
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    Fisher Scientific tris acetate edta tae buffer
    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM <t>bis-tris</t> HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- <t>ethylenediaminetetraacetic</t> acid <t>(EDTA)</t> with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.
    Tris Acetate Edta Tae Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris acetate edta tae buffer/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris acetate edta tae buffer - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Journal: International Journal of Molecular Sciences

    Article Title: Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami

    doi: 10.3390/ijms19092513

    Figure Lengend Snippet: AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Article Snippet: The DNA scaffolds and corresponding staples were mixed in a 1:10 molar ratio, in a 1× tris-acetate-ethylenediaminetetraacetic acid (EDTA) buffer (Fisher Scientific, Hampton, NH, USA), with a pH of 8.3, and a [MgCl2 ] of 12.5 mM.

    Techniques: Incubation

    AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Journal: International Journal of Molecular Sciences

    Article Title: Boron-Implanted Silicon Substrates for Physical Adsorption of DNA Origami

    doi: 10.3390/ijms19092513

    Figure Lengend Snippet: AFM images of DNA origami triangles adsorbed onto boron-implanted silicon substrates ( a – d ) and thermally grown silicon dioxide (SiO 2 ) substrates ( e – h ) as a function of deposition buffer pH. The substrates were cleaned with Piranha + HF. For the pH values below 8.3, the deposition buffer was 10 mM bis-tris HCl with a [MgCl 2 ] of 35 mM. For the pH value of 8.3, 1× tris-acetate- ethylenediaminetetraacetic acid (EDTA) with a [MgCl 2 ] of 35 mM was selected to stay within the buffer range. For all conditions, the deposition incubation time was ~1 h. Scale bars are 500 nm.

    Article Snippet: The DNA scaffolds and corresponding staples were mixed in a 1:10 molar ratio, in a 1× tris-acetate-ethylenediaminetetraacetic acid (EDTA) buffer (Fisher Scientific, Hampton, NH, USA), with a pH of 8.3, and a [MgCl2 ] of 12.5 mM.

    Techniques: Incubation