trioplex real time rt pcr assay  (Thermo Fisher)


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    Thermo Fisher trioplex real time rt pcr assay
    Analytical performance comparison between <t>Trioplex</t> assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per <t>PCR</t> reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Trioplex Real Time Rt Pcr Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trioplex real time rt pcr assay/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    trioplex real time rt pcr assay - by Bioz Stars, 2020-05
    93/100 stars

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    1) Product Images from "Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses"

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    Journal: Nature Communications

    doi: 10.1038/s41467-018-03772-1

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Figure Legend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Techniques Used: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    2) Product Images from "Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses"

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    Journal: Nature Communications

    doi: 10.1038/s41467-018-03772-1

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Figure Legend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Techniques Used: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    Related Articles

    Diagnostic Assay:

    Article Title: Smoking and female sex as key risk factors associated with severe arthralgia in acute and chronic phases of Chikungunya virus infection
    Article Snippet: .. Patient's blood samples were sent to the Public Health Laboratory of the Health Department of the State of Colima (Colima, Mexico) for serum analysis and CHIKV diagnostic by using the Trioplex Real-time RT-PCR Assay (Biosystems™ TaqMan™ Arbovirus Triplex Assay; Thermo Fisher Scientific, Inc., Waltham, MA, USA), according to manufacturer's instructions for detection and differentiation of RNA from dengue, chikungunya or zika ( ). .. The study sample included 140 patients ≥18 years old (average age, 39.9±14.5; 71.4% women) who were positive for CHIKV through RT-qPCR evaluation and provided clinical and personal data during their acute phase, as described above.

    Clone Assay:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Briefly, DNA templates were generated from each dengue virus serotype, DENV-1 Puerto Rico strain 1998, DENV-2 Puerto Rico strain 1998, DENV-3 Puerto Rico strain 2004, DENV-4 Puerto Rico strain 1998, CHIKV Puerto Rico strain 2014, and ZIKV French Polynesia strain 2013 obtained from the CDC Dengue Branch archival reference collection, amplified with the Trioplex real-time RT-PCR assay, and cloned into the pCR-II TOPO TA vector (ThermoFisher). .. Target RNA was transcribed with T7 RNA polymerase using AmpliScribe T7 Flash transcription kit (Lucigen Corp.).

    Amplification:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Briefly, DNA templates were generated from each dengue virus serotype, DENV-1 Puerto Rico strain 1998, DENV-2 Puerto Rico strain 1998, DENV-3 Puerto Rico strain 2004, DENV-4 Puerto Rico strain 1998, CHIKV Puerto Rico strain 2014, and ZIKV French Polynesia strain 2013 obtained from the CDC Dengue Branch archival reference collection, amplified with the Trioplex real-time RT-PCR assay, and cloned into the pCR-II TOPO TA vector (ThermoFisher). .. Target RNA was transcribed with T7 RNA polymerase using AmpliScribe T7 Flash transcription kit (Lucigen Corp.).

    Quantitative RT-PCR:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Briefly, DNA templates were generated from each dengue virus serotype, DENV-1 Puerto Rico strain 1998, DENV-2 Puerto Rico strain 1998, DENV-3 Puerto Rico strain 2004, DENV-4 Puerto Rico strain 1998, CHIKV Puerto Rico strain 2014, and ZIKV French Polynesia strain 2013 obtained from the CDC Dengue Branch archival reference collection, amplified with the Trioplex real-time RT-PCR assay, and cloned into the pCR-II TOPO TA vector (ThermoFisher). .. Target RNA was transcribed with T7 RNA polymerase using AmpliScribe T7 Flash transcription kit (Lucigen Corp.).

    Article Title: Smoking and female sex as key risk factors associated with severe arthralgia in acute and chronic phases of Chikungunya virus infection
    Article Snippet: .. Patient's blood samples were sent to the Public Health Laboratory of the Health Department of the State of Colima (Colima, Mexico) for serum analysis and CHIKV diagnostic by using the Trioplex Real-time RT-PCR Assay (Biosystems™ TaqMan™ Arbovirus Triplex Assay; Thermo Fisher Scientific, Inc., Waltham, MA, USA), according to manufacturer's instructions for detection and differentiation of RNA from dengue, chikungunya or zika ( ). .. The study sample included 140 patients ≥18 years old (average age, 39.9±14.5; 71.4% women) who were positive for CHIKV through RT-qPCR evaluation and provided clinical and personal data during their acute phase, as described above.

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Standard curves were generated and tested with the Trioplex real-time RT-PCR assay on each qPCR instrument, ABI 7500 Fast Dx (ThermoFisher), and QuantStudio Dx (ThermoFisher). .. A linear regression was calculated assuming that every PCR amplicon is equivalent to a complete virus genome and the copy number is directly expressed as GCE per PCR reaction (GCE/rxn).

    Real-time Polymerase Chain Reaction:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Standard curves were generated and tested with the Trioplex real-time RT-PCR assay on each qPCR instrument, ABI 7500 Fast Dx (ThermoFisher), and QuantStudio Dx (ThermoFisher). .. A linear regression was calculated assuming that every PCR amplicon is equivalent to a complete virus genome and the copy number is directly expressed as GCE per PCR reaction (GCE/rxn).

    Generated:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Briefly, DNA templates were generated from each dengue virus serotype, DENV-1 Puerto Rico strain 1998, DENV-2 Puerto Rico strain 1998, DENV-3 Puerto Rico strain 2004, DENV-4 Puerto Rico strain 1998, CHIKV Puerto Rico strain 2014, and ZIKV French Polynesia strain 2013 obtained from the CDC Dengue Branch archival reference collection, amplified with the Trioplex real-time RT-PCR assay, and cloned into the pCR-II TOPO TA vector (ThermoFisher). .. Target RNA was transcribed with T7 RNA polymerase using AmpliScribe T7 Flash transcription kit (Lucigen Corp.).

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Standard curves were generated and tested with the Trioplex real-time RT-PCR assay on each qPCR instrument, ABI 7500 Fast Dx (ThermoFisher), and QuantStudio Dx (ThermoFisher). .. A linear regression was calculated assuming that every PCR amplicon is equivalent to a complete virus genome and the copy number is directly expressed as GCE per PCR reaction (GCE/rxn).

    Polymerase Chain Reaction:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Briefly, DNA templates were generated from each dengue virus serotype, DENV-1 Puerto Rico strain 1998, DENV-2 Puerto Rico strain 1998, DENV-3 Puerto Rico strain 2004, DENV-4 Puerto Rico strain 1998, CHIKV Puerto Rico strain 2014, and ZIKV French Polynesia strain 2013 obtained from the CDC Dengue Branch archival reference collection, amplified with the Trioplex real-time RT-PCR assay, and cloned into the pCR-II TOPO TA vector (ThermoFisher). .. Target RNA was transcribed with T7 RNA polymerase using AmpliScribe T7 Flash transcription kit (Lucigen Corp.).

    Plasmid Preparation:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. Briefly, DNA templates were generated from each dengue virus serotype, DENV-1 Puerto Rico strain 1998, DENV-2 Puerto Rico strain 1998, DENV-3 Puerto Rico strain 2004, DENV-4 Puerto Rico strain 1998, CHIKV Puerto Rico strain 2014, and ZIKV French Polynesia strain 2013 obtained from the CDC Dengue Branch archival reference collection, amplified with the Trioplex real-time RT-PCR assay, and cloned into the pCR-II TOPO TA vector (ThermoFisher). .. Target RNA was transcribed with T7 RNA polymerase using AmpliScribe T7 Flash transcription kit (Lucigen Corp.).

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  • 93
    Thermo Fisher trioplex real time rt pcr assay
    Analytical performance comparison between <t>Trioplex</t> assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per <t>PCR</t> reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Trioplex Real Time Rt Pcr Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trioplex real time rt pcr assay/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    trioplex real time rt pcr assay - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

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    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: Briefly, DNA templates were generated from each dengue virus serotype, DENV-1 Puerto Rico strain 1998, DENV-2 Puerto Rico strain 1998, DENV-3 Puerto Rico strain 2004, DENV-4 Puerto Rico strain 1998, CHIKV Puerto Rico strain 2014, and ZIKV French Polynesia strain 2013 obtained from the CDC Dengue Branch archival reference collection, amplified with the Trioplex real-time RT-PCR assay, and cloned into the pCR-II TOPO TA vector (ThermoFisher).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation