Journal: BMC Biotechnology

Article Title: Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilactici UVA1

doi: 10.1186/1472-6750-7-55

Figure Lengend Snippet: Localization of the pedA gene on the plasmid by curing and Southern blotting . (a) Agarose gel electrophoresis of plasmid DNA. UVA1: P. acidilactici UVA1. UL5: P. acidilactici UL5. bac-: cured derivative of UVA1. M. Supercoiled DNA ladder in kb. ch: chromosomal DNA band. (b) Southern blot DNA hybridization of the DIG labeled pedA -probe with plasmidic DNA from P. acidilactici bac-, UVA1 and UL5. (c) and (d) Agar-well diffusion assay with CFS of cultures of P. acidilactici UVA1 (c) and its cured derivative P. acidilactici bac- (d).

Article Snippet: The low molecular weight DNA ladder and Tridye 100 bp DNA-ladder (New England BioLabs, Ipswich, MA, USA) were used as size standards.

Techniques: Plasmid Preparation, Southern Blot, Agarose Gel Electrophoresis, DNA Hybridization, Labeling, Diffusion-based Assay

Journal: BMC Biotechnology

Article Title: Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilactici UVA1

doi: 10.1186/1472-6750-7-55

Figure Lengend Snippet: Transcription analysis of pedA . pedA -reverse transcription-PCR on cDNA from P. acidilactici bac- (1, 2, 3) or UVA1 (7, 8, 9) after 1 h 30, 2 h 30 and 3 h 30 of growth, respectively, and from P. acidilactici DSM 20284 T (4) or UL5 (6) and P. pentosaceus DSM 20336 T (5) after 2 h 30 of growth. 10: water instead of DNA. lm: low molecular weight DNA ladder (in bp). h: Tridye 100-bp DNA ladder (in bp). Expected product size: 100 bp.

Article Snippet: The low molecular weight DNA ladder and Tridye 100 bp DNA-ladder (New England BioLabs, Ipswich, MA, USA) were used as size standards.

Techniques: Molecular Weight

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy

doi: 10.1007/978-1-62703-462-3_18

Figure Lengend Snippet: The brightfield illuminated (A), UV illuminated (B), and overlaid images (C) of a 0.5% 0.5X TBE agarose ethidium bromide stained gel. Lane 1 is New England Biolab’s Tridye 1kb DNA ladder while lanes 2 and 3 are the passivated and unpassivated particles, respectively. The passivated particles (red arrow) move with quickly into the gel while the unpassivated particles (black arrow) remain near the load well after 35 minutes at 6.5 V/cm.

Article Snippet: New England Biolabs Tridye 1kb DNA ladder.

Techniques: Staining