trichostatin a  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Trichostatin A
    Description:

    Catalog Number:
    ab120850
    Price:
    None
    Buy from Supplier


    Structured Review

    Abcam trichostatin a
    Induction of MT1 receptor and ARHI expression in MCF-7 breast cancer cells in response to <t>trichostatin</t> A and MLT, and the impact of elevated MT1 receptor and MLT on PTX-resistant MCF-7 breast cancer cells. ( A ) MCF-7 breast cancer cells were administered TSA (0.7 μM), MLT (10 nM), or TSA + MLT for 24 h after which the expression of MT1 and ARHI protein levels were measured by Western blot. β-actin was used as a control for equal loading. ( B ) MCF-7/PAC cells were plated at a density of 20 × 10 5 cells per ml in six-well plates and 5 hours after seeding, cells were treated with PTX (5 μM/ml), PTX, TCA (0.7 μM), MLT (10 nM), or TCA + MLT. On specific days total and viable cells counted on a haemocytometer. n = 3 independent studies, and * - p

    https://www.bioz.com/result/trichostatin a/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trichostatin a - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer"

    Article Title: Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer

    Journal: Journal of pineal research

    doi: 10.1111/jpi.12586

    Induction of MT1 receptor and ARHI expression in MCF-7 breast cancer cells in response to trichostatin A and MLT, and the impact of elevated MT1 receptor and MLT on PTX-resistant MCF-7 breast cancer cells. ( A ) MCF-7 breast cancer cells were administered TSA (0.7 μM), MLT (10 nM), or TSA + MLT for 24 h after which the expression of MT1 and ARHI protein levels were measured by Western blot. β-actin was used as a control for equal loading. ( B ) MCF-7/PAC cells were plated at a density of 20 × 10 5 cells per ml in six-well plates and 5 hours after seeding, cells were treated with PTX (5 μM/ml), PTX, TCA (0.7 μM), MLT (10 nM), or TCA + MLT. On specific days total and viable cells counted on a haemocytometer. n = 3 independent studies, and * - p
    Figure Legend Snippet: Induction of MT1 receptor and ARHI expression in MCF-7 breast cancer cells in response to trichostatin A and MLT, and the impact of elevated MT1 receptor and MLT on PTX-resistant MCF-7 breast cancer cells. ( A ) MCF-7 breast cancer cells were administered TSA (0.7 μM), MLT (10 nM), or TSA + MLT for 24 h after which the expression of MT1 and ARHI protein levels were measured by Western blot. β-actin was used as a control for equal loading. ( B ) MCF-7/PAC cells were plated at a density of 20 × 10 5 cells per ml in six-well plates and 5 hours after seeding, cells were treated with PTX (5 μM/ml), PTX, TCA (0.7 μM), MLT (10 nM), or TCA + MLT. On specific days total and viable cells counted on a haemocytometer. n = 3 independent studies, and * - p

    Techniques Used: Expressing, Western Blot

    2) Product Images from "Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness"

    Article Title: Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness

    Journal: Nature Communications

    doi: 10.1038/ncomms11047

    Chromatin accessibility regulated STAT3 dimer-to-tetramer transition (cpCOMB analysis). ( a ) Chromatin organization in HeLa cells stained with Hoechst 33342 after no treatment, 18 h after addition of 400 nM of trichostatin A (loosened chromatin) and 30 min after addition of 5 μg ml −1 of actinomycin D (compacted chromatin). ( b ) Merged STAT3-GFP and STAT3-mCherry intensity images of control, trichostatin A- and actinomycin D-treated live HeLa cells. Scale bar, 5 μm. ( c ) STAT3-GFP and STAT3-mCherry fluorescence intensity within the plane in which the line scan was acquired. The nuclear boundary is indicated by the Hoechst 33342 stain. ( d ) Pseudocoloured brightness maps for STAT3-GFP in control, trichostatin A- and actinomycin D-treated HeLa cells. Dark green pixels represent monomers, light green pixels dimers and red pixels tetramer. ( e ) cpCOMB carpets in control, trichostatin A- and actinomycin D-treated live HeLa cells. White dashed circles highlight tetramer formation. CTYO, cytoplasm; Max, maximum; Min, minimum; NUC, nucleus.
    Figure Legend Snippet: Chromatin accessibility regulated STAT3 dimer-to-tetramer transition (cpCOMB analysis). ( a ) Chromatin organization in HeLa cells stained with Hoechst 33342 after no treatment, 18 h after addition of 400 nM of trichostatin A (loosened chromatin) and 30 min after addition of 5 μg ml −1 of actinomycin D (compacted chromatin). ( b ) Merged STAT3-GFP and STAT3-mCherry intensity images of control, trichostatin A- and actinomycin D-treated live HeLa cells. Scale bar, 5 μm. ( c ) STAT3-GFP and STAT3-mCherry fluorescence intensity within the plane in which the line scan was acquired. The nuclear boundary is indicated by the Hoechst 33342 stain. ( d ) Pseudocoloured brightness maps for STAT3-GFP in control, trichostatin A- and actinomycin D-treated HeLa cells. Dark green pixels represent monomers, light green pixels dimers and red pixels tetramer. ( e ) cpCOMB carpets in control, trichostatin A- and actinomycin D-treated live HeLa cells. White dashed circles highlight tetramer formation. CTYO, cytoplasm; Max, maximum; Min, minimum; NUC, nucleus.

    Techniques Used: Staining, Fluorescence

    3) Product Images from "Involvement of phosphorylated Apis mellifera CREB in gating a honeybee's behavioral response to an external stimulus"

    Article Title: Involvement of phosphorylated Apis mellifera CREB in gating a honeybee's behavioral response to an external stimulus

    Journal: Learning & Memory

    doi: 10.1101/lm.040964.115

    Inhibition of histone deacetylases enhances CS responsiveness. ( A ) Schematic representation of the behavioral experiments. Bees were injected with the histone deacetylase inhibitor Trichostatin A (TSA), the histone acetyl transferase inhibitor, Garcinol or the respective vehicles 2, 5, or 24 h prior to unpaired training ( B ) Percentage of bees responding to the CS during unpaired training after injection of Trichostatin A ( B ) or Garcinol ( C ). (*) Significant differences: P
    Figure Legend Snippet: Inhibition of histone deacetylases enhances CS responsiveness. ( A ) Schematic representation of the behavioral experiments. Bees were injected with the histone deacetylase inhibitor Trichostatin A (TSA), the histone acetyl transferase inhibitor, Garcinol or the respective vehicles 2, 5, or 24 h prior to unpaired training ( B ) Percentage of bees responding to the CS during unpaired training after injection of Trichostatin A ( B ) or Garcinol ( C ). (*) Significant differences: P

    Techniques Used: Inhibition, Injection, Histone Deacetylase Assay

    Related Articles

    Transfection:

    Article Title: Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness
    Article Snippet: .. Loosening of chromatin was carried out by treating the HeLa cells transiently transfected with STAT3-GFP and STAT3-mCherry, with trichostatin A (400 nM, Abcam) for 18 h. Compacting of chromatin was carried out by treating the HeLa cells transiently transfected with STAT3-GFP and STAT3-mCherry, with actinomycin D (5 μg ml−1 , a concentration known to stop class III transcription, Sigma Aldrich) for 30 min. .. The compaction status of chromatin was assessed by staining the DNA of HeLa cells with Hoechst 33342 (1 μg ml−1 , Sigma Aldrich) 15 min before imaging.

    Isolation:

    Article Title: Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer
    Article Snippet: .. Impact of MT1 receptor expression and MLT on ARHI expression and PTX-resistance in breast cancer cells in vitro.To determine if elevated expression of the MT1 MLT receptor could enhance the sensitivity of MCF-7 cells to MLT and enhance MLT mediated expression of ARH1, MCF-7 cells were treated with diluent (0.01% ethanolic cell culture media), MLT (10 nM), trichostatin A (TSA, 0.7 μM), or TSA + MLT for 24 h. Cells were then harvested in lysis buffer after and total cellular protein isolated and analyzed by Western blot analysis for the expression of MT1 (ab87639, Abcam, Cambridge, MA) and ARHI protein levels as described above. ..

    Cell Culture:

    Article Title: Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer
    Article Snippet: .. Impact of MT1 receptor expression and MLT on ARHI expression and PTX-resistance in breast cancer cells in vitro.To determine if elevated expression of the MT1 MLT receptor could enhance the sensitivity of MCF-7 cells to MLT and enhance MLT mediated expression of ARH1, MCF-7 cells were treated with diluent (0.01% ethanolic cell culture media), MLT (10 nM), trichostatin A (TSA, 0.7 μM), or TSA + MLT for 24 h. Cells were then harvested in lysis buffer after and total cellular protein isolated and analyzed by Western blot analysis for the expression of MT1 (ab87639, Abcam, Cambridge, MA) and ARHI protein levels as described above. ..

    Concentration Assay:

    Article Title: Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness
    Article Snippet: .. Loosening of chromatin was carried out by treating the HeLa cells transiently transfected with STAT3-GFP and STAT3-mCherry, with trichostatin A (400 nM, Abcam) for 18 h. Compacting of chromatin was carried out by treating the HeLa cells transiently transfected with STAT3-GFP and STAT3-mCherry, with actinomycin D (5 μg ml−1 , a concentration known to stop class III transcription, Sigma Aldrich) for 30 min. .. The compaction status of chromatin was assessed by staining the DNA of HeLa cells with Hoechst 33342 (1 μg ml−1 , Sigma Aldrich) 15 min before imaging.

    Expressing:

    Article Title: Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer
    Article Snippet: .. Impact of MT1 receptor expression and MLT on ARHI expression and PTX-resistance in breast cancer cells in vitro.To determine if elevated expression of the MT1 MLT receptor could enhance the sensitivity of MCF-7 cells to MLT and enhance MLT mediated expression of ARH1, MCF-7 cells were treated with diluent (0.01% ethanolic cell culture media), MLT (10 nM), trichostatin A (TSA, 0.7 μM), or TSA + MLT for 24 h. Cells were then harvested in lysis buffer after and total cellular protein isolated and analyzed by Western blot analysis for the expression of MT1 (ab87639, Abcam, Cambridge, MA) and ARHI protein levels as described above. ..

    Protease Inhibitor:

    Article Title: Sirt6 inhibition delays the onset of experimental autoimmune encephalomyelitis by reducing dendritic cell migration
    Article Snippet: .. Splenocytes and DC were lysed in 50 mM Tris-HCl (pH 8), 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 10 mM trichostatin A, 10 mM nicotinamide, 0.5 mM DTT, and protease inhibitor cocktail. .. Lysates (30 μg proteins) were loaded on a 10% polyacrylamide gel and separated by SDS-PAGE, and proteins were transferred to nitrocellulose membranes.

    Western Blot:

    Article Title: Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer
    Article Snippet: .. Impact of MT1 receptor expression and MLT on ARHI expression and PTX-resistance in breast cancer cells in vitro.To determine if elevated expression of the MT1 MLT receptor could enhance the sensitivity of MCF-7 cells to MLT and enhance MLT mediated expression of ARH1, MCF-7 cells were treated with diluent (0.01% ethanolic cell culture media), MLT (10 nM), trichostatin A (TSA, 0.7 μM), or TSA + MLT for 24 h. Cells were then harvested in lysis buffer after and total cellular protein isolated and analyzed by Western blot analysis for the expression of MT1 (ab87639, Abcam, Cambridge, MA) and ARHI protein levels as described above. ..

    Lysis:

    Article Title: Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer
    Article Snippet: .. Impact of MT1 receptor expression and MLT on ARHI expression and PTX-resistance in breast cancer cells in vitro.To determine if elevated expression of the MT1 MLT receptor could enhance the sensitivity of MCF-7 cells to MLT and enhance MLT mediated expression of ARH1, MCF-7 cells were treated with diluent (0.01% ethanolic cell culture media), MLT (10 nM), trichostatin A (TSA, 0.7 μM), or TSA + MLT for 24 h. Cells were then harvested in lysis buffer after and total cellular protein isolated and analyzed by Western blot analysis for the expression of MT1 (ab87639, Abcam, Cambridge, MA) and ARHI protein levels as described above. ..

    Injection:

    Article Title: Involvement of phosphorylated Apis mellifera CREB in gating a honeybee's behavioral response to an external stimulus
    Article Snippet: .. To examine the role of histone acetylation, bees were injected 2, 5, or 24 h prior to the unpaired training with 6 mM Garcinol (Abcam; dissolved in 100% DMSO), 1.65 mM Trichostatin A (TSA, Abcam; dissolved in 20% DMSO in PBS) or the appropriate solvent as control. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Abcam trichostatin a
    Induction of MT1 receptor and ARHI expression in MCF-7 breast cancer cells in response to <t>trichostatin</t> A and MLT, and the impact of elevated MT1 receptor and MLT on PTX-resistant MCF-7 breast cancer cells. ( A ) MCF-7 breast cancer cells were administered TSA (0.7 μM), MLT (10 nM), or TSA + MLT for 24 h after which the expression of MT1 and ARHI protein levels were measured by Western blot. β-actin was used as a control for equal loading. ( B ) MCF-7/PAC cells were plated at a density of 20 × 10 5 cells per ml in six-well plates and 5 hours after seeding, cells were treated with PTX (5 μM/ml), PTX, TCA (0.7 μM), MLT (10 nM), or TCA + MLT. On specific days total and viable cells counted on a haemocytometer. n = 3 independent studies, and * - p
    Trichostatin A, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trichostatin a/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trichostatin a - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    99
    Abcam dcfda cellular ros detection assay
    Dynamics of intracellular calcium and reactive oxygen species <t>(ROS)</t> in hTDCs cultured under short-term magnetic stimulation. ( a – d ) Detection of intracellular calcium (green) determined using the fluorescent dye Fluo-3 AM. Scale bar, 20 µm. ( e , f ) Quantification of fluorescence signal of intracellular calcium (cyt Ca 2+ ). A minimum of 35 cells were analysed per condition and data are expressed as scattered dot plot with mean. ( g , h ) ROS determination according to the <t>DCFDA</t> assay and results are expressed as mean ± SEM (n = 9 experimental replicates from 3 biological replicates).
    Dcfda Cellular Ros Detection Assay, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dcfda cellular ros detection assay/product/Abcam
    Average 99 stars, based on 103 article reviews
    Price from $9.99 to $1999.99
    dcfda cellular ros detection assay - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    sirt1  (Abcam)
    85
    Abcam sirt1
    Resveratrol (for 48 h) significantly decreases steady-state mitochondrial O 2 −  production ( A ; assessed by MitoSox fluorescence) in cultured coronary arterial endothelial cells (CAECs). Overexpression of silent information regulator 2/sirtuin 1
    Sirt1, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirt1/product/Abcam
    Average 85 stars, based on 142 article reviews
    Price from $9.99 to $1999.99
    sirt1 - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Induction of MT1 receptor and ARHI expression in MCF-7 breast cancer cells in response to trichostatin A and MLT, and the impact of elevated MT1 receptor and MLT on PTX-resistant MCF-7 breast cancer cells. ( A ) MCF-7 breast cancer cells were administered TSA (0.7 μM), MLT (10 nM), or TSA + MLT for 24 h after which the expression of MT1 and ARHI protein levels were measured by Western blot. β-actin was used as a control for equal loading. ( B ) MCF-7/PAC cells were plated at a density of 20 × 10 5 cells per ml in six-well plates and 5 hours after seeding, cells were treated with PTX (5 μM/ml), PTX, TCA (0.7 μM), MLT (10 nM), or TCA + MLT. On specific days total and viable cells counted on a haemocytometer. n = 3 independent studies, and * - p

    Journal: Journal of pineal research

    Article Title: Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer

    doi: 10.1111/jpi.12586

    Figure Lengend Snippet: Induction of MT1 receptor and ARHI expression in MCF-7 breast cancer cells in response to trichostatin A and MLT, and the impact of elevated MT1 receptor and MLT on PTX-resistant MCF-7 breast cancer cells. ( A ) MCF-7 breast cancer cells were administered TSA (0.7 μM), MLT (10 nM), or TSA + MLT for 24 h after which the expression of MT1 and ARHI protein levels were measured by Western blot. β-actin was used as a control for equal loading. ( B ) MCF-7/PAC cells were plated at a density of 20 × 10 5 cells per ml in six-well plates and 5 hours after seeding, cells were treated with PTX (5 μM/ml), PTX, TCA (0.7 μM), MLT (10 nM), or TCA + MLT. On specific days total and viable cells counted on a haemocytometer. n = 3 independent studies, and * - p

    Article Snippet: Impact of MT1 receptor expression and MLT on ARHI expression and PTX-resistance in breast cancer cells in vitro.To determine if elevated expression of the MT1 MLT receptor could enhance the sensitivity of MCF-7 cells to MLT and enhance MLT mediated expression of ARH1, MCF-7 cells were treated with diluent (0.01% ethanolic cell culture media), MLT (10 nM), trichostatin A (TSA, 0.7 μM), or TSA + MLT for 24 h. Cells were then harvested in lysis buffer after and total cellular protein isolated and analyzed by Western blot analysis for the expression of MT1 (ab87639, Abcam, Cambridge, MA) and ARHI protein levels as described above.

    Techniques: Expressing, Western Blot

    Dynamics of intracellular calcium and reactive oxygen species (ROS) in hTDCs cultured under short-term magnetic stimulation. ( a – d ) Detection of intracellular calcium (green) determined using the fluorescent dye Fluo-3 AM. Scale bar, 20 µm. ( e , f ) Quantification of fluorescence signal of intracellular calcium (cyt Ca 2+ ). A minimum of 35 cells were analysed per condition and data are expressed as scattered dot plot with mean. ( g , h ) ROS determination according to the DCFDA assay and results are expressed as mean ± SEM (n = 9 experimental replicates from 3 biological replicates).

    Journal: Scientific Reports

    Article Title: Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis

    doi: 10.1038/s41598-017-11253-6

    Figure Lengend Snippet: Dynamics of intracellular calcium and reactive oxygen species (ROS) in hTDCs cultured under short-term magnetic stimulation. ( a – d ) Detection of intracellular calcium (green) determined using the fluorescent dye Fluo-3 AM. Scale bar, 20 µm. ( e , f ) Quantification of fluorescence signal of intracellular calcium (cyt Ca 2+ ). A minimum of 35 cells were analysed per condition and data are expressed as scattered dot plot with mean. ( g , h ) ROS determination according to the DCFDA assay and results are expressed as mean ± SEM (n = 9 experimental replicates from 3 biological replicates).

    Article Snippet: Reactive oxygen species (ROS) ROS was quantified via DCFDA Cellular ROS Detection Assay (ab113851, Abcam).

    Techniques: Cell Culture, Fluorescence

    Reactive Oxygen Species (ROS) measured via 2′,7′-dichlorofluorescin diacetate (DCFDA) fluorophore in U87-MG ( A ) and MU1454 ( B ) after treatment with antioxidants (Vitamin D [Vit D], Lipoic acid [LA], or Melatonin [Mel]) and/or temozolomide (TMZ) at 100 uM. Dosages of antioxidants are specified on the graph. Positive control was 55 μM of tert-butylhydroperoxide (TBHP).

    Journal: Medicines

    Article Title: Do Anti-Oxidants Vitamin D3, Melatonin, and Alpha-Lipoic Acid Have Synergistic Effects with Temozolomide on Cultured Glioblastoma Cells?

    doi: 10.3390/medicines5020058

    Figure Lengend Snippet: Reactive Oxygen Species (ROS) measured via 2′,7′-dichlorofluorescin diacetate (DCFDA) fluorophore in U87-MG ( A ) and MU1454 ( B ) after treatment with antioxidants (Vitamin D [Vit D], Lipoic acid [LA], or Melatonin [Mel]) and/or temozolomide (TMZ) at 100 uM. Dosages of antioxidants are specified on the graph. Positive control was 55 μM of tert-butylhydroperoxide (TBHP).

    Article Snippet: Measurement of ROS Production Reactive oxidative species (ROS) production was measured by using the DCFDA Cellular ROS Detection Assay Kit from AbCam (ab113851).

    Techniques: Positive Control

    Resveratrol (for 48 h) significantly decreases steady-state mitochondrial O 2 −  production ( A ; assessed by MitoSox fluorescence) in cultured coronary arterial endothelial cells (CAECs). Overexpression of silent information regulator 2/sirtuin 1

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Resveratrol attenuates mitochondrial oxidative stress in coronary arterial endothelial cells

    doi: 10.1152/ajpheart.00375.2009

    Figure Lengend Snippet: Resveratrol (for 48 h) significantly decreases steady-state mitochondrial O 2 − production ( A ; assessed by MitoSox fluorescence) in cultured coronary arterial endothelial cells (CAECs). Overexpression of silent information regulator 2/sirtuin 1

    Article Snippet: In brief, this assay is based on the principle that upon NAD-dependent deacetylation of the specific substrate by SIRT1 (in the presence of trichostatin A, a potent inhibitor of SIRT1-independent histone deacetylases), the fluorosubstrate peptide is cleaved by a lysyl endopeptidase, separating the quencher from the fluorophore.

    Techniques: Fluorescence, Cell Culture, Over Expression

    A : original Western blot and densitometric results show that resveratrol (RES) induces MnSOD expression in CAECs. Knockdown of SIRT1 (siRNA) prevents the effect of resveratrol, whereas SIRT1 overexpression (Overexp) substantially augments expression.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Resveratrol attenuates mitochondrial oxidative stress in coronary arterial endothelial cells

    doi: 10.1152/ajpheart.00375.2009

    Figure Lengend Snippet: A : original Western blot and densitometric results show that resveratrol (RES) induces MnSOD expression in CAECs. Knockdown of SIRT1 (siRNA) prevents the effect of resveratrol, whereas SIRT1 overexpression (Overexp) substantially augments expression.

    Article Snippet: In brief, this assay is based on the principle that upon NAD-dependent deacetylation of the specific substrate by SIRT1 (in the presence of trichostatin A, a potent inhibitor of SIRT1-independent histone deacetylases), the fluorosubstrate peptide is cleaved by a lysyl endopeptidase, separating the quencher from the fluorophore.

    Techniques: Western Blot, Expressing, Over Expression