Structured Review

Millipore triacsin c
HASMC were treated with PA (250 µM) for 1 day in the presence or absence of EPA (15 µM), <t>triacsin</t> <t>C</t> (Tri C; 2 µM) or hypoestoxide (Hypo; 100 µM). The expression of BMP-2, Msx2 and osteopontin (OPN) mRNAs was determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by arbitrary unit (AU) are presented as the mean ± S.E. (n = 4). #p<0.05, ###p<0.001 vs. control, **p<0.01, ***p<0.001 vs. PA.
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "Palmitic Acid Induces Osteoblastic Differentiation in Vascular Smooth Muscle Cells through ACSL3 and NF-κB, Novel Targets of Eicosapentaenoic Acid"

Article Title: Palmitic Acid Induces Osteoblastic Differentiation in Vascular Smooth Muscle Cells through ACSL3 and NF-κB, Novel Targets of Eicosapentaenoic Acid

Journal: PLoS ONE

doi: 10.1371/journal.pone.0068197

HASMC were treated with PA (250 µM) for 1 day in the presence or absence of EPA (15 µM), triacsin C (Tri C; 2 µM) or hypoestoxide (Hypo; 100 µM). The expression of BMP-2, Msx2 and osteopontin (OPN) mRNAs was determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by arbitrary unit (AU) are presented as the mean ± S.E. (n = 4). #p<0.05, ###p<0.001 vs. control, **p<0.01, ***p<0.001 vs. PA.
Figure Legend Snippet: HASMC were treated with PA (250 µM) for 1 day in the presence or absence of EPA (15 µM), triacsin C (Tri C; 2 µM) or hypoestoxide (Hypo; 100 µM). The expression of BMP-2, Msx2 and osteopontin (OPN) mRNAs was determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by arbitrary unit (AU) are presented as the mean ± S.E. (n = 4). #p<0.05, ###p<0.001 vs. control, **p<0.01, ***p<0.001 vs. PA.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction


Structured Review

Millipore triacsin c
LRAT-mediated LD formation in the absence of preexisting LDs. (A) Confocal microscopy of CHO-k1 cells expressing LRAT-GFP. After preincubation with medium containing 1% FBS, cells were incubated overnight with or without 200 µM OA or 20 µM ROH in the presence or absence of 1 µg/ml triacsin C. Cells were stained with DAPI (blue) and LD540 (green). Scale bars indicate 10 µm (zoomed regions are 10 µm wide). CON, control. (B) Quantification of predominant RE species by LC-MS/MS of WT and 4Δ yeast cells expressing LRAT 2 h after incubation in the presence or absence of 2 mM ROH. Amounts are expressed in pmol RE per μg protein. Bar plot indicates mean ± SD. (C) Widefield microscopy of Erg6-mCherry expressing WT and 4Δ yeast cells, with or without expressing LRAT, 2 h after incubation with or without 2 mM ROH (− or +). Images of UV autofluorescence (green), Erg6-mCherry (red), and brightfield were taken. Scale bars indicate 5 µm. (D) EM of WT and 4Δ yeast cells, with or without expressing LRAT, incubated with or without 2 mM ROH. M, mitochondria; N, nucleus. Scale bars indicate 250 nm. (E) EM of yft2Δscs3Δ and 4Δ yeast cells expressing LRAT incubated with 2 mM ROH. Scale bars indicate 100 nm. ER is indicated by yellow lines in right panels; LDs are indicated by red lines in right panels. (F) Widefield microscopy of Erg6-mCherry expressing WT yeast cells expressing LRAT. Two time series of UV autofluorescence (white), Erg6-mCherry (red), and brightfield taken 2, 4, 6, and 8 min after addition of 2 mM ROH. Arrows indicate LDs that become UV + over time. Scale bars indicate 5 µm.
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "Retinyl esters form lipid droplets independently of triacylglycerol and seipin"

Article Title: Retinyl esters form lipid droplets independently of triacylglycerol and seipin

Journal: The Journal of Cell Biology

doi: 10.1083/jcb.202011071

LRAT-mediated LD formation in the absence of preexisting LDs. (A) Confocal microscopy of CHO-k1 cells expressing LRAT-GFP. After preincubation with medium containing 1% FBS, cells were incubated overnight with or without 200 µM OA or 20 µM ROH in the presence or absence of 1 µg/ml triacsin C. Cells were stained with DAPI (blue) and LD540 (green). Scale bars indicate 10 µm (zoomed regions are 10 µm wide). CON, control. (B) Quantification of predominant RE species by LC-MS/MS of WT and 4Δ yeast cells expressing LRAT 2 h after incubation in the presence or absence of 2 mM ROH. Amounts are expressed in pmol RE per μg protein. Bar plot indicates mean ± SD. (C) Widefield microscopy of Erg6-mCherry expressing WT and 4Δ yeast cells, with or without expressing LRAT, 2 h after incubation with or without 2 mM ROH (− or +). Images of UV autofluorescence (green), Erg6-mCherry (red), and brightfield were taken. Scale bars indicate 5 µm. (D) EM of WT and 4Δ yeast cells, with or without expressing LRAT, incubated with or without 2 mM ROH. M, mitochondria; N, nucleus. Scale bars indicate 250 nm. (E) EM of yft2Δscs3Δ and 4Δ yeast cells expressing LRAT incubated with 2 mM ROH. Scale bars indicate 100 nm. ER is indicated by yellow lines in right panels; LDs are indicated by red lines in right panels. (F) Widefield microscopy of Erg6-mCherry expressing WT yeast cells expressing LRAT. Two time series of UV autofluorescence (white), Erg6-mCherry (red), and brightfield taken 2, 4, 6, and 8 min after addition of 2 mM ROH. Arrows indicate LDs that become UV + over time. Scale bars indicate 5 µm.
Figure Legend Snippet: LRAT-mediated LD formation in the absence of preexisting LDs. (A) Confocal microscopy of CHO-k1 cells expressing LRAT-GFP. After preincubation with medium containing 1% FBS, cells were incubated overnight with or without 200 µM OA or 20 µM ROH in the presence or absence of 1 µg/ml triacsin C. Cells were stained with DAPI (blue) and LD540 (green). Scale bars indicate 10 µm (zoomed regions are 10 µm wide). CON, control. (B) Quantification of predominant RE species by LC-MS/MS of WT and 4Δ yeast cells expressing LRAT 2 h after incubation in the presence or absence of 2 mM ROH. Amounts are expressed in pmol RE per μg protein. Bar plot indicates mean ± SD. (C) Widefield microscopy of Erg6-mCherry expressing WT and 4Δ yeast cells, with or without expressing LRAT, 2 h after incubation with or without 2 mM ROH (− or +). Images of UV autofluorescence (green), Erg6-mCherry (red), and brightfield were taken. Scale bars indicate 5 µm. (D) EM of WT and 4Δ yeast cells, with or without expressing LRAT, incubated with or without 2 mM ROH. M, mitochondria; N, nucleus. Scale bars indicate 250 nm. (E) EM of yft2Δscs3Δ and 4Δ yeast cells expressing LRAT incubated with 2 mM ROH. Scale bars indicate 100 nm. ER is indicated by yellow lines in right panels; LDs are indicated by red lines in right panels. (F) Widefield microscopy of Erg6-mCherry expressing WT yeast cells expressing LRAT. Two time series of UV autofluorescence (white), Erg6-mCherry (red), and brightfield taken 2, 4, 6, and 8 min after addition of 2 mM ROH. Arrows indicate LDs that become UV + over time. Scale bars indicate 5 µm.

Techniques Used: Confocal Microscopy, Expressing, Incubation, Staining, Liquid Chromatography with Mass Spectroscopy, Microscopy


Structured Review

Millipore triacsin c from streptomyces sp
Oxfenicine (1 mM), or 4-bromo-crotonic acid (50 M) were present in the medium for 2 h whereas <t>triacsin</t> <t>C</t> (5 μM) was present for 24 h before the experiments. The enzyme targets of each inhibitor are illustrated by the FAO scheme on the right (dashed red lines). Cell medium was collected for measurements of acyl- and desacyl-ghrelin. Columns and bars are means ± SEM, number of experiments indicated in brackets. *P < 0.05, **P < 0.01 compared to vehicle (ANOVA followed by Tukey’s multiple comparison test.
Triacsin C From Streptomyces Sp, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c from streptomyces sp/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c from streptomyces sp - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "Targeting Peripheral CB 1 Receptors Reduces Ethanol Intake via a Gut-Brain Axis"

Article Title: Targeting Peripheral CB 1 Receptors Reduces Ethanol Intake via a Gut-Brain Axis

Journal: Cell metabolism

doi: 10.1016/j.cmet.2019.04.012

Oxfenicine (1 mM), or 4-bromo-crotonic acid (50 M) were present in the medium for 2 h whereas triacsin C (5 μM) was present for 24 h before the experiments. The enzyme targets of each inhibitor are illustrated by the FAO scheme on the right (dashed red lines). Cell medium was collected for measurements of acyl- and desacyl-ghrelin. Columns and bars are means ± SEM, number of experiments indicated in brackets. *P < 0.05, **P < 0.01 compared to vehicle (ANOVA followed by Tukey’s multiple comparison test.
Figure Legend Snippet: Oxfenicine (1 mM), or 4-bromo-crotonic acid (50 M) were present in the medium for 2 h whereas triacsin C (5 μM) was present for 24 h before the experiments. The enzyme targets of each inhibitor are illustrated by the FAO scheme on the right (dashed red lines). Cell medium was collected for measurements of acyl- and desacyl-ghrelin. Columns and bars are means ± SEM, number of experiments indicated in brackets. *P < 0.05, **P < 0.01 compared to vehicle (ANOVA followed by Tukey’s multiple comparison test.

Techniques Used:

KEY RESOURCES TABLE
Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: shRNA, Plasmid Preparation, Recombinant, Lysis, Amplification, SYBR Green Assay, Multiplex Assay, Injection, Ethanol Assay, Enzyme-linked Immunosorbent Assay, Mutagenesis, Software


Structured Review

Millipore triacsin c
Excess oleic acid fails to reverse the effect of FR179254 . Lipid droplets are not the targeted process of FR179254. Cells were cultured in normal medium or medium containing excess oleic acid (OA) to induce lipid droplets (A) and then infected and treated as indicated with either FR179254 or <t>Triacsin</t> <t>C</t> (TrC), a fatty acid synthase and lipid droplet inhibitor. (B) Chlamydial growth was assessed at 24 and 48 h post-infection. One set of samples were reactivated (React) by treating with indicated drugs for 24 h, washing three times, and then culturing in the presence or absence of excess oleic acid in fresh medium lacking drugs. Reactivated samples were collected after 24 h (48 h total culture time). All data were normalized to chlamydial growth in untreated cells at 24 h post-infection (arbitrarily 100%).
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "A Functional Slow Recycling Pathway of Transferrin is Required for Growth of Chlamydia"

Article Title: A Functional Slow Recycling Pathway of Transferrin is Required for Growth of Chlamydia

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2010.00112

Excess oleic acid fails to reverse the effect of FR179254 . Lipid droplets are not the targeted process of FR179254. Cells were cultured in normal medium or medium containing excess oleic acid (OA) to induce lipid droplets (A) and then infected and treated as indicated with either FR179254 or Triacsin C (TrC), a fatty acid synthase and lipid droplet inhibitor. (B) Chlamydial growth was assessed at 24 and 48 h post-infection. One set of samples were reactivated (React) by treating with indicated drugs for 24 h, washing three times, and then culturing in the presence or absence of excess oleic acid in fresh medium lacking drugs. Reactivated samples were collected after 24 h (48 h total culture time). All data were normalized to chlamydial growth in untreated cells at 24 h post-infection (arbitrarily 100%).
Figure Legend Snippet: Excess oleic acid fails to reverse the effect of FR179254 . Lipid droplets are not the targeted process of FR179254. Cells were cultured in normal medium or medium containing excess oleic acid (OA) to induce lipid droplets (A) and then infected and treated as indicated with either FR179254 or Triacsin C (TrC), a fatty acid synthase and lipid droplet inhibitor. (B) Chlamydial growth was assessed at 24 and 48 h post-infection. One set of samples were reactivated (React) by treating with indicated drugs for 24 h, washing three times, and then culturing in the presence or absence of excess oleic acid in fresh medium lacking drugs. Reactivated samples were collected after 24 h (48 h total culture time). All data were normalized to chlamydial growth in untreated cells at 24 h post-infection (arbitrarily 100%).

Techniques Used: Cell Culture, Infection


Structured Review

Millipore triacsin c
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Millipore triacsin c
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Millipore triacsin c
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Millipore triacsin c
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Millipore triacsin c
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Millipore triacsin c
Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

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  • 86
    Millipore triacsin c
    HASMC were treated with PA (250 µM) for 1 day in the presence or absence of EPA (15 µM), <t>triacsin</t> <t>C</t> (Tri C; 2 µM) or hypoestoxide (Hypo; 100 µM). The expression of BMP-2, Msx2 and osteopontin (OPN) mRNAs was determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by arbitrary unit (AU) are presented as the mean ± S.E. (n = 4). #p<0.05, ###p<0.001 vs. control, **p<0.01, ***p<0.001 vs. PA.
    Triacsin C, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    86
    Millipore triacsin c from streptomyces sp
    Oxfenicine (1 mM), or 4-bromo-crotonic acid (50 M) were present in the medium for 2 h whereas <t>triacsin</t> <t>C</t> (5 μM) was present for 24 h before the experiments. The enzyme targets of each inhibitor are illustrated by the FAO scheme on the right (dashed red lines). Cell medium was collected for measurements of acyl- and desacyl-ghrelin. Columns and bars are means ± SEM, number of experiments indicated in brackets. *P < 0.05, **P < 0.01 compared to vehicle (ANOVA followed by Tukey’s multiple comparison test.
    Triacsin C From Streptomyces Sp, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c from streptomyces sp/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c from streptomyces sp - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

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    HASMC were treated with PA (250 µM) for 1 day in the presence or absence of EPA (15 µM), triacsin C (Tri C; 2 µM) or hypoestoxide (Hypo; 100 µM). The expression of BMP-2, Msx2 and osteopontin (OPN) mRNAs was determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by arbitrary unit (AU) are presented as the mean ± S.E. (n = 4). #p<0.05, ###p<0.001 vs. control, **p<0.01, ***p<0.001 vs. PA.

    Journal: PLoS ONE

    Article Title: Palmitic Acid Induces Osteoblastic Differentiation in Vascular Smooth Muscle Cells through ACSL3 and NF-κB, Novel Targets of Eicosapentaenoic Acid

    doi: 10.1371/journal.pone.0068197

    Figure Lengend Snippet: HASMC were treated with PA (250 µM) for 1 day in the presence or absence of EPA (15 µM), triacsin C (Tri C; 2 µM) or hypoestoxide (Hypo; 100 µM). The expression of BMP-2, Msx2 and osteopontin (OPN) mRNAs was determined by real-time PCR and normalized to GAPDH. The control values are shown as 1.0. All values expressed by arbitrary unit (AU) are presented as the mean ± S.E. (n = 4). #p<0.05, ###p<0.001 vs. control, **p<0.01, ***p<0.001 vs. PA.

    Article Snippet: For experiments related to calcium deposition and caspase activation, HASMC were grown until confluent in Humedia-SG2 medium, and then treated with 1 mM PA in the presence or absence of 15 µM EPA, 2 µM triacsin C, 100 µM hypoestoxide and 100 µM Z-VAD-FMK (Calbiochem) in Humedia-SG2 medium containing with 0.3% BSA and 3% PBS for 1 day.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Oxfenicine (1 mM), or 4-bromo-crotonic acid (50 M) were present in the medium for 2 h whereas triacsin C (5 μM) was present for 24 h before the experiments. The enzyme targets of each inhibitor are illustrated by the FAO scheme on the right (dashed red lines). Cell medium was collected for measurements of acyl- and desacyl-ghrelin. Columns and bars are means ± SEM, number of experiments indicated in brackets. *P < 0.05, **P < 0.01 compared to vehicle (ANOVA followed by Tukey’s multiple comparison test.

    Journal: Cell metabolism

    Article Title: Targeting Peripheral CB 1 Receptors Reduces Ethanol Intake via a Gut-Brain Axis

    doi: 10.1016/j.cmet.2019.04.012

    Figure Lengend Snippet: Oxfenicine (1 mM), or 4-bromo-crotonic acid (50 M) were present in the medium for 2 h whereas triacsin C (5 μM) was present for 24 h before the experiments. The enzyme targets of each inhibitor are illustrated by the FAO scheme on the right (dashed red lines). Cell medium was collected for measurements of acyl- and desacyl-ghrelin. Columns and bars are means ± SEM, number of experiments indicated in brackets. *P < 0.05, **P < 0.01 compared to vehicle (ANOVA followed by Tukey’s multiple comparison test.

    Article Snippet: Triacsin C from Streptomyces sp. , MilliporeSigma , T4540.

    Techniques:

    KEY RESOURCES TABLE

    Journal: Cell metabolism

    Article Title: Targeting Peripheral CB 1 Receptors Reduces Ethanol Intake via a Gut-Brain Axis

    doi: 10.1016/j.cmet.2019.04.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Triacsin C from Streptomyces sp. , MilliporeSigma , T4540.

    Techniques: shRNA, Plasmid Preparation, Recombinant, Lysis, Amplification, SYBR Green Assay, Multiplex Assay, Injection, Ethanol Assay, Enzyme-linked Immunosorbent Assay, Mutagenesis, Software