Structured Review

Biomol GmbH triacsin c
(A) The effects of agents that elevate cAMP on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM for 4–10 experiments for Plin1 cultures incubated for 1 hour with: Control media (Co); 10 µg/ml Isoproterenol (Iso); 10 µM Forskolin (Forsk); 10 µM Adenosine (Aden); or 500 µM 8-(4-Chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-CPT-cAMP). The lower case letter a indicates values that are statistically different from control values, p<0.001. (B) The effects of kinase and triglyceride synthesis inhibitors on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM (3 experiments) for Plin1 cultures preincubated without addition (-), or with 20 µM H89, 1 mM AICAR, or 8 µM <t>Triacsin</t> <t>C</t> (TriC) for 30 minutes, and then incubated in control media (Co) or with media containing 10 µg/ml isoproterenol (Iso) for 1 hour. Statistical significance is indicated by lower case letters a and b: a, values differ from their respective controls (p<0.001); b, values for cultures that were preincubated with H89 before being treated with isoproterenol differ from those that were not pretreated before isoproterenol (p<0.001).
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Dynamics and Molecular Determinants of Cytoplasmic Lipid Droplet Clustering and Dispersion"

Article Title: Dynamics and Molecular Determinants of Cytoplasmic Lipid Droplet Clustering and Dispersion

Journal: PLoS ONE

doi: 10.1371/journal.pone.0066837

(A) The effects of agents that elevate cAMP on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM for 4–10 experiments for Plin1 cultures incubated for 1 hour with: Control media (Co); 10 µg/ml Isoproterenol (Iso); 10 µM Forskolin (Forsk); 10 µM Adenosine (Aden); or 500 µM 8-(4-Chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-CPT-cAMP). The lower case letter a indicates values that are statistically different from control values, p<0.001. (B) The effects of kinase and triglyceride synthesis inhibitors on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM (3 experiments) for Plin1 cultures preincubated without addition (-), or with 20 µM H89, 1 mM AICAR, or 8 µM Triacsin C (TriC) for 30 minutes, and then incubated in control media (Co) or with media containing 10 µg/ml isoproterenol (Iso) for 1 hour. Statistical significance is indicated by lower case letters a and b: a, values differ from their respective controls (p<0.001); b, values for cultures that were preincubated with H89 before being treated with isoproterenol differ from those that were not pretreated before isoproterenol (p<0.001).
Figure Legend Snippet: (A) The effects of agents that elevate cAMP on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM for 4–10 experiments for Plin1 cultures incubated for 1 hour with: Control media (Co); 10 µg/ml Isoproterenol (Iso); 10 µM Forskolin (Forsk); 10 µM Adenosine (Aden); or 500 µM 8-(4-Chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-CPT-cAMP). The lower case letter a indicates values that are statistically different from control values, p<0.001. (B) The effects of kinase and triglyceride synthesis inhibitors on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM (3 experiments) for Plin1 cultures preincubated without addition (-), or with 20 µM H89, 1 mM AICAR, or 8 µM Triacsin C (TriC) for 30 minutes, and then incubated in control media (Co) or with media containing 10 µg/ml isoproterenol (Iso) for 1 hour. Statistical significance is indicated by lower case letters a and b: a, values differ from their respective controls (p<0.001); b, values for cultures that were preincubated with H89 before being treated with isoproterenol differ from those that were not pretreated before isoproterenol (p<0.001).

Techniques Used: Incubation


Structured Review

Biomol GmbH triacsin c
(A) Representative autoradiogram of TLC plate of WT and KO NSC34 cells treated with 14 C-OA for the indicated time points. (B) Quantification of TAG accumulation over time. n = 3, unpaired t test, * P < 0.05, ** P < 0.01. (C) Representative autoradiogram of TLC plates showing TAG levels of WT and KO cells or media (m) after 24-h treatment with 14 C-OA (0 h) followed by 6-h incubation with <t>triacsin</t> <t>C</t> (6 h). (D) Quantification of the TAG content of experiments shown in (C), normalized to WT cells at 0 h. n = 3, unpaired t test, ** P < 0.01, *** P < 0.001. (E) Quantification of the decrease in TAG content after 6 h of triacsin C treatment in WT and KO cells (dotted line represents levels at the beginning of treatment, 0 h, of the respective genotype). (F) WT and spastin KO NSC34 cells were either mock treated or down-regulated with two different siRNAs for Atgl (si Atgl 1 and si Atgl 2) and PLIN2 levels were analyzed by Western blot. (G) Quantification of PLIN2 level normalized to GAPDH are expressed relative to WT mock sample (n = 5, paired t test * P < 0.05, ** P < 0.01). See also .
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Microtubule-dependent and independent roles of spastin in lipid droplet dispersion and biogenesis"

Article Title: Microtubule-dependent and independent roles of spastin in lipid droplet dispersion and biogenesis

Journal: Life Science Alliance

doi: 10.26508/lsa.202000715

(A) Representative autoradiogram of TLC plate of WT and KO NSC34 cells treated with 14 C-OA for the indicated time points. (B) Quantification of TAG accumulation over time. n = 3, unpaired t test, * P < 0.05, ** P < 0.01. (C) Representative autoradiogram of TLC plates showing TAG levels of WT and KO cells or media (m) after 24-h treatment with 14 C-OA (0 h) followed by 6-h incubation with triacsin C (6 h). (D) Quantification of the TAG content of experiments shown in (C), normalized to WT cells at 0 h. n = 3, unpaired t test, ** P < 0.01, *** P < 0.001. (E) Quantification of the decrease in TAG content after 6 h of triacsin C treatment in WT and KO cells (dotted line represents levels at the beginning of treatment, 0 h, of the respective genotype). (F) WT and spastin KO NSC34 cells were either mock treated or down-regulated with two different siRNAs for Atgl (si Atgl 1 and si Atgl 2) and PLIN2 levels were analyzed by Western blot. (G) Quantification of PLIN2 level normalized to GAPDH are expressed relative to WT mock sample (n = 5, paired t test * P < 0.05, ** P < 0.01). See also .
Figure Legend Snippet: (A) Representative autoradiogram of TLC plate of WT and KO NSC34 cells treated with 14 C-OA for the indicated time points. (B) Quantification of TAG accumulation over time. n = 3, unpaired t test, * P < 0.05, ** P < 0.01. (C) Representative autoradiogram of TLC plates showing TAG levels of WT and KO cells or media (m) after 24-h treatment with 14 C-OA (0 h) followed by 6-h incubation with triacsin C (6 h). (D) Quantification of the TAG content of experiments shown in (C), normalized to WT cells at 0 h. n = 3, unpaired t test, ** P < 0.01, *** P < 0.001. (E) Quantification of the decrease in TAG content after 6 h of triacsin C treatment in WT and KO cells (dotted line represents levels at the beginning of treatment, 0 h, of the respective genotype). (F) WT and spastin KO NSC34 cells were either mock treated or down-regulated with two different siRNAs for Atgl (si Atgl 1 and si Atgl 2) and PLIN2 levels were analyzed by Western blot. (G) Quantification of PLIN2 level normalized to GAPDH are expressed relative to WT mock sample (n = 5, paired t test * P < 0.05, ** P < 0.01). See also .

Techniques Used: Incubation, Western Blot


Structured Review

Biomol GmbH triacsin c
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Structured Review

Biomol GmbH triacsin c
After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of <t>triacsin</t> <t>C</t> (10 μmol/l) before measuring GSIS (5 determinations/experiment). *p<0.05, **p<0.01 for effect of 17 mmol/l glucose effect. ## p<0.01; ### p<0.001 for the chronically elevated glucose effect.
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Biomol GmbH
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1) Product Images from "SREBP1 is required for the induction by glucose of pancreatic β-cell genes involved in glucose sensing"

Article Title: SREBP1 is required for the induction by glucose of pancreatic β-cell genes involved in glucose sensing

Journal: Journal of lipid research

doi: 10.1194/jlr.M700533-JLR200

After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before measuring GSIS (5 determinations/experiment). *p<0.05, **p<0.01 for effect of 17 mmol/l glucose effect. ## p<0.01; ### p<0.001 for the chronically elevated glucose effect.
Figure Legend Snippet: After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before measuring GSIS (5 determinations/experiment). *p<0.05, **p<0.01 for effect of 17 mmol/l glucose effect. ## p<0.01; ### p<0.001 for the chronically elevated glucose effect.

Techniques Used: Isolation, Cell Culture

After isolation, islets from wild type mice (n=3/genotype) were cultured for 96h at 8 or 30 mmol/l glucose in the presence or absence of Triacsin C (10 μmol/l). TG content was measured as described in Material and Methods. p<0.05 for the effect of chronically elevated [glucose]. # p<0.05 for the Triacsin C effect.
Figure Legend Snippet: After isolation, islets from wild type mice (n=3/genotype) were cultured for 96h at 8 or 30 mmol/l glucose in the presence or absence of Triacsin C (10 μmol/l). TG content was measured as described in Material and Methods. p<0.05 for the effect of chronically elevated [glucose]. # p<0.05 for the Triacsin C effect.

Techniques Used: Isolation, Cell Culture

(A-F): After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before RNA extraction. *p<0.05 for the chronically elevated glucose effect. # p<0.05 for the triacsin C effect.
Figure Legend Snippet: (A-F): After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before RNA extraction. *p<0.05 for the chronically elevated glucose effect. # p<0.05 for the triacsin C effect.

Techniques Used: Isolation, Cell Culture, RNA Extraction


Structured Review

Biomol GmbH triacsin c
After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of <t>triacsin</t> <t>C</t> (10 μmol/l) before measuring GSIS (5 determinations/experiment). *p<0.05, **p<0.01 for effect of 17 mmol/l glucose effect. ## p<0.01; ### p<0.001 for the chronically elevated glucose effect.
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Biomol GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

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1) Product Images from "SREBP1 is required for the induction by glucose of pancreatic β-cell genes involved in glucose sensing"

Article Title: SREBP1 is required for the induction by glucose of pancreatic β-cell genes involved in glucose sensing

Journal: Journal of lipid research

doi: 10.1194/jlr.M700533-JLR200

After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before measuring GSIS (5 determinations/experiment). *p<0.05, **p<0.01 for effect of 17 mmol/l glucose effect. ## p<0.01; ### p<0.001 for the chronically elevated glucose effect.
Figure Legend Snippet: After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before measuring GSIS (5 determinations/experiment). *p<0.05, **p<0.01 for effect of 17 mmol/l glucose effect. ## p<0.01; ### p<0.001 for the chronically elevated glucose effect.

Techniques Used: Isolation, Cell Culture

After isolation, islets from wild type mice (n=3/genotype) were cultured for 96h at 8 or 30 mmol/l glucose in the presence or absence of Triacsin C (10 μmol/l). TG content was measured as described in Material and Methods. p<0.05 for the effect of chronically elevated [glucose]. # p<0.05 for the Triacsin C effect.
Figure Legend Snippet: After isolation, islets from wild type mice (n=3/genotype) were cultured for 96h at 8 or 30 mmol/l glucose in the presence or absence of Triacsin C (10 μmol/l). TG content was measured as described in Material and Methods. p<0.05 for the effect of chronically elevated [glucose]. # p<0.05 for the Triacsin C effect.

Techniques Used: Isolation, Cell Culture

(A-F): After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before RNA extraction. *p<0.05 for the chronically elevated glucose effect. # p<0.05 for the triacsin C effect.
Figure Legend Snippet: (A-F): After isolation, islets from wild type mice (n=3/genotype) were cultured for 96 h at 8 or 30 mmol/l glucose in the presence or absence of triacsin C (10 μmol/l) before RNA extraction. *p<0.05 for the chronically elevated glucose effect. # p<0.05 for the triacsin C effect.

Techniques Used: Isolation, Cell Culture, RNA Extraction


Structured Review

Biomol GmbH triacsin c
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Biomol GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

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Structured Review

Biomol GmbH triacsin c
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Biomol GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

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Structured Review

Biomol GmbH triacsin c
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Biomol GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

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Structured Review

Biomol GmbH triacsin c
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Biomol GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
86/100 stars

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Structured Review

Biomol GmbH triacsin c
Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triacsin c/product/Biomol GmbH
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
triacsin c - by Bioz Stars, 2023-02
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    Biomol GmbH triacsin c
    (A) The effects of agents that elevate cAMP on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM for 4–10 experiments for Plin1 cultures incubated for 1 hour with: Control media (Co); 10 µg/ml Isoproterenol (Iso); 10 µM Forskolin (Forsk); 10 µM Adenosine (Aden); or 500 µM 8-(4-Chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-CPT-cAMP). The lower case letter a indicates values that are statistically different from control values, p<0.001. (B) The effects of kinase and triglyceride synthesis inhibitors on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM (3 experiments) for Plin1 cultures preincubated without addition (-), or with 20 µM H89, 1 mM AICAR, or 8 µM <t>Triacsin</t> <t>C</t> (TriC) for 30 minutes, and then incubated in control media (Co) or with media containing 10 µg/ml isoproterenol (Iso) for 1 hour. Statistical significance is indicated by lower case letters a and b: a, values differ from their respective controls (p<0.001); b, values for cultures that were preincubated with H89 before being treated with isoproterenol differ from those that were not pretreated before isoproterenol (p<0.001).
    Triacsin C, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triacsin c/product/Biomol GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triacsin c - by Bioz Stars, 2023-02
    86/100 stars
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    (A) The effects of agents that elevate cAMP on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM for 4–10 experiments for Plin1 cultures incubated for 1 hour with: Control media (Co); 10 µg/ml Isoproterenol (Iso); 10 µM Forskolin (Forsk); 10 µM Adenosine (Aden); or 500 µM 8-(4-Chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-CPT-cAMP). The lower case letter a indicates values that are statistically different from control values, p<0.001. (B) The effects of kinase and triglyceride synthesis inhibitors on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM (3 experiments) for Plin1 cultures preincubated without addition (-), or with 20 µM H89, 1 mM AICAR, or 8 µM Triacsin C (TriC) for 30 minutes, and then incubated in control media (Co) or with media containing 10 µg/ml isoproterenol (Iso) for 1 hour. Statistical significance is indicated by lower case letters a and b: a, values differ from their respective controls (p<0.001); b, values for cultures that were preincubated with H89 before being treated with isoproterenol differ from those that were not pretreated before isoproterenol (p<0.001).

    Journal: PLoS ONE

    Article Title: Dynamics and Molecular Determinants of Cytoplasmic Lipid Droplet Clustering and Dispersion

    doi: 10.1371/journal.pone.0066837

    Figure Lengend Snippet: (A) The effects of agents that elevate cAMP on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM for 4–10 experiments for Plin1 cultures incubated for 1 hour with: Control media (Co); 10 µg/ml Isoproterenol (Iso); 10 µM Forskolin (Forsk); 10 µM Adenosine (Aden); or 500 µM 8-(4-Chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-CPT-cAMP). The lower case letter a indicates values that are statistically different from control values, p<0.001. (B) The effects of kinase and triglyceride synthesis inhibitors on CLD dispersion as determined by the number of Plin1 objects/cell. The values are means ± SEM (3 experiments) for Plin1 cultures preincubated without addition (-), or with 20 µM H89, 1 mM AICAR, or 8 µM Triacsin C (TriC) for 30 minutes, and then incubated in control media (Co) or with media containing 10 µg/ml isoproterenol (Iso) for 1 hour. Statistical significance is indicated by lower case letters a and b: a, values differ from their respective controls (p<0.001); b, values for cultures that were preincubated with H89 before being treated with isoproterenol differ from those that were not pretreated before isoproterenol (p<0.001).

    Article Snippet: Triacsin C was purchased from BioMol (Plymouth Meeting, PA), H-89 was purchased from InvivoGen (San Diego, CA), and 8-(4-Chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate was purchased from Tocris Bioscience (Bristol, UK).

    Techniques: Incubation