tri reagent  (Millipore)

 
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    Name:
    TRI Reagent
    Description:
    TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA DNA and protein from liquid samples of human animal plant yeast bacterial and viral origin A convenient single step liquid phase separation results in the simultaneous isolation of RNA DNA and protein This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation and permits fast and efficient processing of liquid samples TRI Reagent LS performs well with large or small sample volumes and many samples can be simultaneously extracted TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution When a biological sample is homogenized or lysed with it and chloroform or 1 bromo 3 chloropropane is added the mixture separates into 3 phases an aqueous phase containing RNA the interphase containing DNA and an organic phase containing proteins Each component can then be isolated after separating the phases 0 75 ml of TRI Reagent LS processes 0 25 ml of a liquid sample such as amniotic fluid This is one of the most effective methods for isolating total RNA from fresh samples in only one hour The procedure is very effective for isolating RNA molecules of all types from 0 1 15 kb in length The resulting RNA is intact with little or no contaminating DNA and protein
    Catalog Number:
    T3934
    Price:
    None
    Applications:
    TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
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    Structured Review

    Millipore tri reagent
    TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA DNA and protein from liquid samples of human animal plant yeast bacterial and viral origin A convenient single step liquid phase separation results in the simultaneous isolation of RNA DNA and protein This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation and permits fast and efficient processing of liquid samples TRI Reagent LS performs well with large or small sample volumes and many samples can be simultaneously extracted TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution When a biological sample is homogenized or lysed with it and chloroform or 1 bromo 3 chloropropane is added the mixture separates into 3 phases an aqueous phase containing RNA the interphase containing DNA and an organic phase containing proteins Each component can then be isolated after separating the phases 0 75 ml of TRI Reagent LS processes 0 25 ml of a liquid sample such as amniotic fluid This is one of the most effective methods for isolating total RNA from fresh samples in only one hour The procedure is very effective for isolating RNA molecules of all types from 0 1 15 kb in length The resulting RNA is intact with little or no contaminating DNA and protein
    https://www.bioz.com/result/tri reagent/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tri reagent - by Bioz Stars, 2021-05
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Valproic acid inhibits A? production, neuritic plaque formation, and behavioral deficits in Alzheimer's disease mouse models
    Article Snippet: .. RNA was isolated from cells using TRI-Reagent (Sigma-Aldrich). .. PowerScript reverse transcription (Invitrogen) was used to synthesize the first-strand cDNA from an equal amount of the RNA sample following the manufacturer's instruction.

    Article Title: Self-Protection against Gliotoxin--A Component of the Gliotoxin Biosynthetic Cluster, GliT, Completely Protects Aspergillus fumigatus Against Exogenous Gliotoxin
    Article Snippet: In order to obtain homokaryotic transformants, colonies from single homokaryotic spores were picked and single genomic integration was confirmed by PCR (data not shown) and Southern blot analysis. .. Northern analysis RNA was isolated using TRI-Reagent (Sigma-Aldrich). .. Equal concentrations of total RNA (10 µg) were size-separated on 1.2% agarose-2.2 M formaldehyde gels and blotted onto Hybond N+ membranes (Amersham Biosciences).

    Article Title: The effect of fucoxanthin rich power on the lipid metabolism in rats with a high fat diet
    Article Snippet: .. Total RNA isolation Total liver RNA was isolated using TRI-reagent (Sigma aldrich, MO, USA) according to the manufacturer's protocol [ ]. ..

    Article Title: Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells
    Article Snippet: .. Quantitative Polymerase Chain Reaction (PCR) Total RNA was isolated using the trizol method from HMCs, according to the manufacturer's protocol (Sigma-Aldrich, T9424). ..

    Article Title: High maysin corn silk extract reduces body weight and fat deposition in C57BL/6J mice fed high-fat diets
    Article Snippet: Total RNA isolation, reverse transcription, and real-time PCR The detailed methods for total RNA isolation, reverse transcription, and real-time PCR for measuring mRNA expression of genes related to adipocyte differentiation, triglyceride synthesis, and fat oxidation in liver or epididymal fat pad were performed as previously described [ ]. .. Total liver or adipose tissue RNA was isolated using TRI-reagent (Sigma Aldrich, MO, USA) according to the manufacturer's protocol. ..

    Expressing:

    Article Title: Adhesive protein-mediated cross-talk between Candida albicans and Porphyromonas gingivalis in dual species biofilm protects the anaerobic bacterium in unfavorable oxic environment
    Article Snippet: The biofilms were then stained with CFW and analyzed microscopically using an excitation wavelength of 405 nm to visualize CFW-stained fungal cells, and 488 nm to detect bacterial cells labeled with CFSE. .. Expression of genes encoding selected yeast virulence factors After 3 hours of mutual contact between both microbes under anoxia and normoxia, total RNA was extracted with a TRI® Reagent (Sigma Aldrich) in accordance with the protocol provided by the manufacturer. .. For cDNA synthesis, each 20-µl reaction mixture consisted of 2 µg of RNA, 0.5 µg of oligonucleotide (dT)18 primer and 200 U of MLV reverse transcriptase (Moloney murine leukemia virus reverse transcriptase; Promega, Madison, WI).

    RNA Extraction:

    Article Title: Exercise Training Induced Improvement in Skeletal Muscle PGC-1α Mediated Fat Metabolism is Independent of Dietary Glycemic Index
    Article Snippet: .. RNA Extraction Muscle RNA was extracted with TRI Reagent (Sigma, St Louis, MO). .. Briefly, 10–20 mg of tissue was homogenized in TRI Reagent with repetitive short bursts, and incubated at room temperature for 5–10 minutes, followed by centrifugation at 12,000xg for 10 minutes at 4°C.

    Article Title: RNA extraction from self-assembling peptide hydrogels to allow qPCR analysis of encapsulated cells
    Article Snippet: For cell-only controls, cells were treated as above but re-suspended in 90 μL of Dulbecco’s phosphate-buffered saline (dPBS) (without Ca2+ and Mg2+ ; Sigma-Aldrich, Dorset, UK) with no media incubation. .. RNA extraction RNA was extracted using the following commercial kits: RNeasy Mini Kit® (Qiagen, Manchester, UK) or TRI Reagent® (Sigma-Aldrich, Dorset, UK). .. The cell culture medium was then removed before each hydrogel was washed three times in dPBS for 5 minutes.

    Northern Blot:

    Article Title: Self-Protection against Gliotoxin--A Component of the Gliotoxin Biosynthetic Cluster, GliT, Completely Protects Aspergillus fumigatus Against Exogenous Gliotoxin
    Article Snippet: In order to obtain homokaryotic transformants, colonies from single homokaryotic spores were picked and single genomic integration was confirmed by PCR (data not shown) and Southern blot analysis. .. Northern analysis RNA was isolated using TRI-Reagent (Sigma-Aldrich). .. Equal concentrations of total RNA (10 µg) were size-separated on 1.2% agarose-2.2 M formaldehyde gels and blotted onto Hybond N+ membranes (Amersham Biosciences).

    Real-time Polymerase Chain Reaction:

    Article Title: Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells
    Article Snippet: .. Quantitative Polymerase Chain Reaction (PCR) Total RNA was isolated using the trizol method from HMCs, according to the manufacturer's protocol (Sigma-Aldrich, T9424). ..

    Polymerase Chain Reaction:

    Article Title: Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells
    Article Snippet: .. Quantitative Polymerase Chain Reaction (PCR) Total RNA was isolated using the trizol method from HMCs, according to the manufacturer's protocol (Sigma-Aldrich, T9424). ..

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  • 97
    Millipore tri reagent
    Effect of fucoxanthin on mRNA expression of CPT1 in the liver of rats. Total <t>RNA</t> was isolated using <t>TRI-reagenet</t> and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. CPT1, Carnitine palmitoyltransferase-1.
    Tri Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tri reagent/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tri reagent - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

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    Effect of fucoxanthin on mRNA expression of CPT1 in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. CPT1, Carnitine palmitoyltransferase-1.

    Journal: Nutrition Research and Practice

    Article Title: The effect of fucoxanthin rich power on the lipid metabolism in rats with a high fat diet

    doi: 10.4162/nrp.2013.7.4.287

    Figure Lengend Snippet: Effect of fucoxanthin on mRNA expression of CPT1 in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. CPT1, Carnitine palmitoyltransferase-1.

    Article Snippet: Total RNA isolation Total liver RNA was isolated using TRI-reagent (Sigma aldrich, MO, USA) according to the manufacturer's protocol [ ].

    Techniques: Expressing, Isolation, Synthesized, Polymerase Chain Reaction, SYBR Green Assay

    Effect of fucoxanthin on mRNA expression of transcription factor and enzymes related to lipid metabolism in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. SREBP-1C, Sterol regulatory element binding protein; G6PDH, Glucose-6-phosphate dehydrogenase; FAS, Fatty acid synthase; ACC, Acetyl-CoA carboxylase.

    Journal: Nutrition Research and Practice

    Article Title: The effect of fucoxanthin rich power on the lipid metabolism in rats with a high fat diet

    doi: 10.4162/nrp.2013.7.4.287

    Figure Lengend Snippet: Effect of fucoxanthin on mRNA expression of transcription factor and enzymes related to lipid metabolism in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. SREBP-1C, Sterol regulatory element binding protein; G6PDH, Glucose-6-phosphate dehydrogenase; FAS, Fatty acid synthase; ACC, Acetyl-CoA carboxylase.

    Article Snippet: Total RNA isolation Total liver RNA was isolated using TRI-reagent (Sigma aldrich, MO, USA) according to the manufacturer's protocol [ ].

    Techniques: Expressing, Isolation, Synthesized, Polymerase Chain Reaction, SYBR Green Assay, Binding Assay

    Effect of fucoxanthin on mRNA expression of enzymes related to cholesterol synthesis in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. HMG-CoA, Hydroxy-3-methylglutaryl coenzyme A; ACAT, Acyl-CoA cholesterol acyltransferase; LCAT, lecithin-cholesterol acyltransferase.

    Journal: Nutrition Research and Practice

    Article Title: The effect of fucoxanthin rich power on the lipid metabolism in rats with a high fat diet

    doi: 10.4162/nrp.2013.7.4.287

    Figure Lengend Snippet: Effect of fucoxanthin on mRNA expression of enzymes related to cholesterol synthesis in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. HMG-CoA, Hydroxy-3-methylglutaryl coenzyme A; ACAT, Acyl-CoA cholesterol acyltransferase; LCAT, lecithin-cholesterol acyltransferase.

    Article Snippet: Total RNA isolation Total liver RNA was isolated using TRI-reagent (Sigma aldrich, MO, USA) according to the manufacturer's protocol [ ].

    Techniques: Expressing, Isolation, Synthesized, Polymerase Chain Reaction, SYBR Green Assay

    Effect of fucoxanthin on the mRNA expression of CYP7A1 in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. CYP7A1, Cholesterol 7α-hydroxylase1.

    Journal: Nutrition Research and Practice

    Article Title: The effect of fucoxanthin rich power on the lipid metabolism in rats with a high fat diet

    doi: 10.4162/nrp.2013.7.4.287

    Figure Lengend Snippet: Effect of fucoxanthin on the mRNA expression of CYP7A1 in the liver of rats. Total RNA was isolated using TRI-reagenet and cDNA was synthesized using 3 µg of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± SD of three independent experiments. Different letters above each bar indicate significant differences among groups at α = 0.05 as determined by Duncan's multiple range test. CYP7A1, Cholesterol 7α-hydroxylase1.

    Article Snippet: Total RNA isolation Total liver RNA was isolated using TRI-reagent (Sigma aldrich, MO, USA) according to the manufacturer's protocol [ ].

    Techniques: Expressing, Isolation, Synthesized, Polymerase Chain Reaction, SYBR Green Assay

    ERK 1/2 inhibition attenuated the CsA/SRL-induced alterations in HMCs. HMCs were grown to confluency and pretreated with UO126 10 μ M for 1-hour prior to treatment with vehicle control, 4.2 μ M CsA, 1 μ M SRL, or CsA/SRL 4.2 μ M + 1 μ M, for 24 hours. phase contrast micrographs were taken using a CCD camera mounted on a Nikon microscope. (a) Magnification 200X. HMC viability was measured using the Resazurin Conversion Assay. (b) TGF- β 1 secretion was detected using a TGF- β 1 ELISA. (c) 24-hour CTGF gene expression and 24-hour MMP-9 gene expression. Gene expression was detected by quantitative PCR. Each column represents the mean ± SEM of a minimum of 3 independent experiments performed in duplicate. Data was analysed by ANOVA and comparisons between control and multiple treatment groups were Mmade using the Dunnet Posttest.* P

    Journal: Journal of Transplantation

    Article Title: Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells

    doi: 10.1155/2012/980910

    Figure Lengend Snippet: ERK 1/2 inhibition attenuated the CsA/SRL-induced alterations in HMCs. HMCs were grown to confluency and pretreated with UO126 10 μ M for 1-hour prior to treatment with vehicle control, 4.2 μ M CsA, 1 μ M SRL, or CsA/SRL 4.2 μ M + 1 μ M, for 24 hours. phase contrast micrographs were taken using a CCD camera mounted on a Nikon microscope. (a) Magnification 200X. HMC viability was measured using the Resazurin Conversion Assay. (b) TGF- β 1 secretion was detected using a TGF- β 1 ELISA. (c) 24-hour CTGF gene expression and 24-hour MMP-9 gene expression. Gene expression was detected by quantitative PCR. Each column represents the mean ± SEM of a minimum of 3 independent experiments performed in duplicate. Data was analysed by ANOVA and comparisons between control and multiple treatment groups were Mmade using the Dunnet Posttest.* P

    Article Snippet: Quantitative Polymerase Chain Reaction (PCR) Total RNA was isolated using the trizol method from HMCs, according to the manufacturer's protocol (Sigma-Aldrich, T9424).

    Techniques: Inhibition, Microscopy, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction

    CsA/SRL cotreatment increased CTGF and decreased MMP-9 gene expression in HMCs. HMCs were grown to confluency and treated with vehicle, 4.2 μ M CsA, 1 μ M SRL or CsA/SRL, 4.2 μ M CsA + 1 μ M SRL for 24 hours. CTGF and MMP-9 expression was detected by quantitative PCR. (a) 24-hour CTGF gene expression and (b) MMP-9 gene expression. Each column represents the mean ± SEM of a minimum of 3 independent experiments performed in duplicate. Data was analysed by ANOVA and comparisons between control and multiple treatment groups were made using the Dunnet posttest. *Indicates statistical difference compared to control. * P

    Journal: Journal of Transplantation

    Article Title: Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells

    doi: 10.1155/2012/980910

    Figure Lengend Snippet: CsA/SRL cotreatment increased CTGF and decreased MMP-9 gene expression in HMCs. HMCs were grown to confluency and treated with vehicle, 4.2 μ M CsA, 1 μ M SRL or CsA/SRL, 4.2 μ M CsA + 1 μ M SRL for 24 hours. CTGF and MMP-9 expression was detected by quantitative PCR. (a) 24-hour CTGF gene expression and (b) MMP-9 gene expression. Each column represents the mean ± SEM of a minimum of 3 independent experiments performed in duplicate. Data was analysed by ANOVA and comparisons between control and multiple treatment groups were made using the Dunnet posttest. *Indicates statistical difference compared to control. * P

    Article Snippet: Quantitative Polymerase Chain Reaction (PCR) Total RNA was isolated using the trizol method from HMCs, according to the manufacturer's protocol (Sigma-Aldrich, T9424).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    The effect of exercise combined with high and low glycemic diet interventions on the expression of genes involved in fat transport and oxidation. A biopsy of the vastus lateralis muscle was performed after an overnight fast before and after the lifestyle intervention and RNA was extracted from 10–20 mg of the sample using the TRI-method. The expression of genes was analyzed by quantitative–real time PCR analysis, calculated by the ΔΔCt method, and expressed as fold induction relative to pre-exercise. Data are mean ± SE. *Significant difference pre- vs. post-intervention ( P

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Exercise Training Induced Improvement in Skeletal Muscle PGC-1α Mediated Fat Metabolism is Independent of Dietary Glycemic Index

    doi: 10.1002/oby.21799

    Figure Lengend Snippet: The effect of exercise combined with high and low glycemic diet interventions on the expression of genes involved in fat transport and oxidation. A biopsy of the vastus lateralis muscle was performed after an overnight fast before and after the lifestyle intervention and RNA was extracted from 10–20 mg of the sample using the TRI-method. The expression of genes was analyzed by quantitative–real time PCR analysis, calculated by the ΔΔCt method, and expressed as fold induction relative to pre-exercise. Data are mean ± SE. *Significant difference pre- vs. post-intervention ( P

    Article Snippet: RNA Extraction Muscle RNA was extracted with TRI Reagent (Sigma, St Louis, MO).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Transformation of A. nidulans and S. cerevisiae with gliT facilitates resistance to exogenous gliotoxin. (A) Southern analysis confirms that two A. nidulans transformants contain gliT ( An gliT 7 and 8) compared to A. nidulans wild-type (An-wt). wt: A. fumigatus (positive control for gliT ). Genomic DNA was digested with Pst I and probed for the presence of A. fumigatus gliT . (B) Northern analysis of gliT in An-wt, An gliT (7 and 8) and A. fumigatus wild-type (wt). RNA was isolated using TRI-reagent and 10 µg of total RNA were probed with a gliT -specific probe. (C) Plate assay of A. nidulans wild-type and A. nidulans expressing gliT ( An gliT 7 and 8). A. nidulans wild-type and An gliT (10 4 ) conidia were dotted on AMM and on AMM containing gliotoxin (50 µg/ml). Growth was monitored over a period of 72 h at 37°C. Genotypes of strains are described in Table 1 . (D) GliT confers gliotoxin resistance on S. cerevisiae . Spots represent equal numbers of yeast cells plated onto medium containing gliotoxin at the concentrations indicated. Plates were incubated at 30°C for two days followed by a further three days at room temperature.

    Journal: PLoS Pathogens

    Article Title: Self-Protection against Gliotoxin--A Component of the Gliotoxin Biosynthetic Cluster, GliT, Completely Protects Aspergillus fumigatus Against Exogenous Gliotoxin

    doi: 10.1371/journal.ppat.1000952

    Figure Lengend Snippet: Transformation of A. nidulans and S. cerevisiae with gliT facilitates resistance to exogenous gliotoxin. (A) Southern analysis confirms that two A. nidulans transformants contain gliT ( An gliT 7 and 8) compared to A. nidulans wild-type (An-wt). wt: A. fumigatus (positive control for gliT ). Genomic DNA was digested with Pst I and probed for the presence of A. fumigatus gliT . (B) Northern analysis of gliT in An-wt, An gliT (7 and 8) and A. fumigatus wild-type (wt). RNA was isolated using TRI-reagent and 10 µg of total RNA were probed with a gliT -specific probe. (C) Plate assay of A. nidulans wild-type and A. nidulans expressing gliT ( An gliT 7 and 8). A. nidulans wild-type and An gliT (10 4 ) conidia were dotted on AMM and on AMM containing gliotoxin (50 µg/ml). Growth was monitored over a period of 72 h at 37°C. Genotypes of strains are described in Table 1 . (D) GliT confers gliotoxin resistance on S. cerevisiae . Spots represent equal numbers of yeast cells plated onto medium containing gliotoxin at the concentrations indicated. Plates were incubated at 30°C for two days followed by a further three days at room temperature.

    Article Snippet: Northern analysis RNA was isolated using TRI-Reagent (Sigma-Aldrich).

    Techniques: Transformation Assay, Positive Control, Northern Blot, Isolation, Expressing, Incubation