treponema denticola strains atcc 35404  (ATCC)


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    Name:
    Treponema denticola
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    Catalog Number:
    35404
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    Structured Review

    ATCC treponema denticola strains atcc 35404
    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. <t>denticola</t> <t>ATCC</t> 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

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    treponema denticola strains atcc 35404 - by Bioz Stars, 2020-04
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    Related Products / Commonly Used Together

    hemolysin genes
    holt s c. cloning
    karunakaran t

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    1) Product Images from "Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola"

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola

    Journal: Infection and Immunity

    doi: 10.1128/IAI.69.7.4465-4472.2001

    Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.
    Figure Legend Snippet: Northern blot analyses of the hbpA and hbpB mRNAs. (A) Total RNA was isolated from T. denticola ATCC 35404 grown in GM-1 medium plus 200 μM BPD or in GM-1 medium. The indicated amounts of RNA were separated on a 1% agarose gel and stained with ethidium bromide. (B and C) The RNA was then transferred to a membrane and hybridized with an hbpA probe (a 333-bp Hin dIII fragment internal to the hbpA gene) (B) or an hbpB probe (a PCR product internal to the hbpB gene) (C). Each sample in panel C contained 3 μg of total RNA.

    Techniques Used: Northern Blot, Isolation, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction

    Related Articles

    Clone Assay:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: .. Karunakaran T, Holt S C. Cloning and expression of hemolysin genes from Treponema denticola strains ATCC 35404 (TD-4) and human clinical isolate GM-1 in Escherichia coli . ..

    DNA Synthesis:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: Research with other cationic peptides suggests that secondary targets, such as cytochromes or components of DNA synthesis, may be necessary for killing ( , ). .. To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ).

    Blocking Assay:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE. .. The nitrocellulose sheets were dried and then incubated for 4 h at 25°C in a blocking solution (1% bovine serum albumin in 10 mM Tris-buffered saline).

    Two Tailed Test:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. Two independent experiments demonstrate that T. denticola was not killed by hβD-2 under these assay conditions: untreated treponemes produced 7.2 × 106 ± 4.9 × 106 CFU/ml, while treponemes incubated in the presence of peptides produced 1.8 × 107 ± 1.4 × 107 CFU/ml ( P = 0.55, Student's two-tailed t test assuming unequal variances).

    Activity Assay:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: To test the hypothesis that T. denticola can degrade hβD-2, thus preventing its activity, we examined the effect of protease inhibitors on survival of T. denticola exposed to hβD-2. .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ).

    Article Title: Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity
    Article Snippet: Strain BEM9 was used as the platform for engineering the butyrate pathway containing atoB from E. coli , hbd, crt, ptb and buk from C. acetobutylicum (ATCC 824), and ter from Treponema denticola (ATCC 35404). .. The Ter protein from ATCC 35404 had a slightly higher specific activity (82 units; µmoles per min. mg protein) compared to the 73 units reported in the literature for the protein from T. denticola ATCC 35405 [ ] when both proteins were expressed and purified from E. coli .

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: A 1/10 volume of Alamar Blue (Biosource, Camarillo, Calif.) was then added, and the bacteria were incubated for an additional 20 h. E. coli was used as a control for hβD-2 activity. .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. E. coli incubated in GM-1 medium was used as a control for hβD-2 activity and was quantitated by serial dilution on LB agar.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: This method has been used successfully to monitor antimicrobial activity against Mycobacterium tuberculosis ( ). .. As there was little difference in hβD-2 sensitivity among strains, T. denticola ATCC 35404 was used for all experiments.

    Expressing:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: Chu L, Holt S C. Purification and characterization of a 45 kDa hemolysin from Treponema denticola ATCC 35404. .. Chu L, Song M, Holt S C. Effect of iron regulation on expression and hemin-binding function of outer-sheath proteins from Treponema denticola .

    Article Title: Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment
    Article Snippet: Paragraph title: Conservation and expression of oppA in oral treponemes. ... The OppA peptide of T. denticola ATCC 35404 had a slightly higher apparent molecular mass than that of the other OppA peptides (Fig. B, lane 2).

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: .. Karunakaran T, Holt S C. Cloning and expression of hemolysin genes from Treponema denticola strains ATCC 35404 (TD-4) and human clinical isolate GM-1 in Escherichia coli . ..

    Western Blot:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: Paragraph title: Western immunoblotting. ... T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE.

    Southern Blot:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: The msp gene, which encodes the major OMP of T. denticola , differs between strains, although there are regions of strong identity among the msp genes from the different strains ( ); by Southern blot analysis, there are at least two restriction fragment length polymorphism patterns around the msp gene among strains. .. The restriction fragment length polymorphism pattern around the hemolysin gene from T. denticola ATCC 35404 is different from that of the hemolysin gene from a clinical isolate, GM-1 ( ).

    Protease Inhibitor:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). .. Similar results were obtained with other strains of T. denticola and with aprotinin (Sigma) and Complete protease inhibitor cocktail (Roche Molecular Biochemicals, Indianapolis, Ind.) (data not shown).

    Infection:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE. .. Transferred proteins were probed with 1:2,000 dilutions of three individual mouse sera, from (i) primary infection, (ii) primary infection plus reinfection, and (iii) active immunization plus primary infection, by overnight incubation at 25°C.

    Sequencing:

    Article Title: Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity
    Article Snippet: Strain BEM9 was used as the platform for engineering the butyrate pathway containing atoB from E. coli , hbd, crt, ptb and buk from C. acetobutylicum (ATCC 824), and ter from Treponema denticola (ATCC 35404). .. Based on DNA sequence, Ter from strain ATCC 35404 had two amino acid changes (D354E and S358G) compared to the enzyme from T. denticola ATCC 35405.

    Recombinant:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). .. The activity of the protease inhibitors was confirmed by measuring the degradation of recombinant interleukin 8 (R & D Systems, Minneapolis, Minn.) by T. denticola in the presence or absence of the protease inhibitors; chymostatin effectively inhibited > 90% of interleukin 8 degradation by T. denticola (data not shown).

    Isolation:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: T. denticola ATCC 35404 (TD-4) was used as the source of genomic DNA and in the RNA studies. .. T. denticola strains GM-1 and MS25 were originally isolated from human periodontal pockets ( ).

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: Karunakaran T, Holt S C. Cloning and expression of hemolysin genes from Treponema denticola strains ATCC 35404 (TD-4) and human clinical isolate GM-1 in Escherichia coli . .. Karunakaran T, Madden T, Kuramitsu H. Isolation and characterization of a hemin-regulated gene, hemR , from Porphyromonas gingivalis .

    Microscopy:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Purification:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: .. Chu L, Holt S C. Purification and characterization of a 45 kDa hemolysin from Treponema denticola ATCC 35404. ..

    Article Title: Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity
    Article Snippet: Strain BEM9 was used as the platform for engineering the butyrate pathway containing atoB from E. coli , hbd, crt, ptb and buk from C. acetobutylicum (ATCC 824), and ter from Treponema denticola (ATCC 35404). .. The Ter protein from ATCC 35404 had a slightly higher specific activity (82 units; µmoles per min. mg protein) compared to the 73 units reported in the literature for the protein from T. denticola ATCC 35405 [ ] when both proteins were expressed and purified from E. coli .

    Metabolic Labelling:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: These results confirm that under these assay conditions, T. denticola remains metabolically active after treatment with hβD-2. .. To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: .. T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE. .. The proteins were electrophoretically transferred onto nitrocellulose paper (0.25-μm pore size; Schleicher & Schuell, Inc., Keene, N.H.) by using 25 mM Tris–192 mM glycine–20% methanol buffer (pH 8.3) at 8 V/cm for 3 h at 4°C ( ).

    Activated Clotting Time Assay:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: In some cases, transcriptional regulation by iron is due to Fur or DtxR proteins, which act as iron-responsive DNA-binding repressor proteins ( , , , ). .. The restriction fragment length polymorphism pattern around the hemolysin gene from T. denticola ATCC 35404 is different from that of the hemolysin gene from a clinical isolate, GM-1 ( ).

    Functional Assay:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: A functional tonB gene is required for both utilization of heme and virulence expression by Haemophilus influenzae type b. Infect Immun. .. Karunakaran T, Holt S C. Cloning and expression of hemolysin genes from Treponema denticola strains ATCC 35404 (TD-4) and human clinical isolate GM-1 in Escherichia coli .

    Binding Assay:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: Although the transcription of hbpA is regulated by iron, we did not find any matches to the Fur or DtxR consensus binding sequences in the hbpA promoter region. .. The restriction fragment length polymorphism pattern around the hemolysin gene from T. denticola ATCC 35404 is different from that of the hemolysin gene from a clinical isolate, GM-1 ( ).

    Incubation:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE. .. The nitrocellulose sheets were dried and then incubated for 4 h at 25°C in a blocking solution (1% bovine serum albumin in 10 mM Tris-buffered saline).

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. E. coli incubated in GM-1 medium was used as a control for hβD-2 activity and was quantitated by serial dilution on LB agar.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: As there was little difference in hβD-2 sensitivity among strains, T. denticola ATCC 35404 was used for all experiments. .. Organisms (final concentration, 107 /ml) were incubated with 10 μg of hβD-2 per ml or 0.1% sodium dodecyl sulfate for 4 h at 37°C and 5% CO2 .

    Produced:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. Two independent experiments demonstrate that T. denticola was not killed by hβD-2 under these assay conditions: untreated treponemes produced 7.2 × 106 ± 4.9 × 106 CFU/ml, while treponemes incubated in the presence of peptides produced 1.8 × 107 ± 1.4 × 107 CFU/ml ( P = 0.55, Student's two-tailed t test assuming unequal variances).

    Concentration Assay:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: Organisms (final concentration, 107 /ml) were incubated with 10 μg of hβD-2 per ml or 0.1% sodium dodecyl sulfate for 4 h at 37°C and 5% CO2 . .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2.

    Serial Dilution:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. E. coli incubated in GM-1 medium was used as a control for hβD-2 activity and was quantitated by serial dilution on LB agar.

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    ATCC t denticola atcc 35405
    Construction of T. <t>denticola</t> mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain <t>ATCC</t> 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .
    T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola atcc 35405/product/ATCC
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    t denticola atcc 35405 - by Bioz Stars, 2020-04
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    99
    ATCC t denticola atcc 35404
    T. <t>denticola</t> proteases are not responsible for resistance to hβD-2. (A) T. denticola <t>ATCC</t> 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    T Denticola Atcc 35404, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola atcc 35404/product/ATCC
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t denticola atcc 35404 - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Journal: Journal of microbiological methods

    Article Title: A simplified erythromycin resistance cassette for Treponema denticola mutagenesis

    doi: 10.1016/j.mimet.2010.07.020

    Figure Lengend Snippet: Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Article Snippet: T. denticola ATCC 35405 (the Type strain, which has been passaged extensively in various laboratories) was electroporated with the resulting linear DNA s and plated in NOS-GN soft agar medium containing erythromycin (40 μg ml−1 , Sigma Chemical Co., St. Louis, MO) as described previously.

    Techniques:

    Mean areas of lesions at infection sites after challenge with T. denticola ATCC 35405 and prtP mutant K1. Mice were injected with live T. denticola ATCC 35405 (open bars) or mutant K1 (hatched bars), and lesion areas were determined at the indicated times following infection.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Mean areas of lesions at infection sites after challenge with T. denticola ATCC 35405 and prtP mutant K1. Mice were injected with live T. denticola ATCC 35405 (open bars) or mutant K1 (hatched bars), and lesion areas were determined at the indicated times following infection.

    Article Snippet: Zymography with gelatin as a substrate (Fig. ) indicated that a proteolytic band of approximately 100 kDa could be readily detected for wild-type strain ATCC 35405 but that this band was absent in mutant K1.

    Techniques: Infection, Mutagenesis, Mouse Assay, Injection

    Immunoblot analysis of T. denticola ATCC 35405 and K1 with anti- T. denticola ATCC 35405 whole-cell serum. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Immunoblot analysis of T. denticola ATCC 35405 and K1 with anti- T. denticola ATCC 35405 whole-cell serum. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Article Snippet: Zymography with gelatin as a substrate (Fig. ) indicated that a proteolytic band of approximately 100 kDa could be readily detected for wild-type strain ATCC 35405 but that this band was absent in mutant K1.

    Techniques:

    Southern blot analysis of T. denticola ATCC 35405 and prtP mutant K1. Chromosomal DNAs from T. denticola ATCC 35405 (lanes 1 and 3) and prtP mutant K1 (lanes 2 and 4) were digested with Hin dIII and hybridized with a digoxigenin-labeled Kpn I- Pst I fragment from the prtP gene (lanes 1 and 2) or the Kpn I- Bam HI fragment from pVA2198 (lanes 3 and 4). Numbers at right are kilobase pairs.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Southern blot analysis of T. denticola ATCC 35405 and prtP mutant K1. Chromosomal DNAs from T. denticola ATCC 35405 (lanes 1 and 3) and prtP mutant K1 (lanes 2 and 4) were digested with Hin dIII and hybridized with a digoxigenin-labeled Kpn I- Pst I fragment from the prtP gene (lanes 1 and 2) or the Kpn I- Bam HI fragment from pVA2198 (lanes 3 and 4). Numbers at right are kilobase pairs.

    Article Snippet: Zymography with gelatin as a substrate (Fig. ) indicated that a proteolytic band of approximately 100 kDa could be readily detected for wild-type strain ATCC 35405 but that this band was absent in mutant K1.

    Techniques: Southern Blot, Mutagenesis, Labeling

    Gelatin zymography of sonicates of T. denticola ATCC 35405 (lane 1) and K1 (lane 2).

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Gelatin zymography of sonicates of T. denticola ATCC 35405 (lane 1) and K1 (lane 2).

    Article Snippet: Zymography with gelatin as a substrate (Fig. ) indicated that a proteolytic band of approximately 100 kDa could be readily detected for wild-type strain ATCC 35405 but that this band was absent in mutant K1.

    Techniques: Zymography

    SDS-PAGE analysis of sonicates of T. denticola ATCC 35405 and K1. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). After electrophoresis, the gel was stained with Coomassie brilliant blue R-250. Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: SDS-PAGE analysis of sonicates of T. denticola ATCC 35405 and K1. Samples from lanes 1 to 4 were treated with PMSF. Lanes 1 and 5, T. denticola ATCC 35405 (without boiling); lanes 2 and 6, T. denticola K1 (without boiling); lanes 3 and 7, T. denticola ATCC 35405 (with boiling); lanes 4 and 8, T. denticola K1 (with boiling). After electrophoresis, the gel was stained with Coomassie brilliant blue R-250. Arrowheads indicate the high-molecular-mass oligomeric protein and the Msp band.

    Article Snippet: Zymography with gelatin as a substrate (Fig. ) indicated that a proteolytic band of approximately 100 kDa could be readily detected for wild-type strain ATCC 35405 but that this band was absent in mutant K1.

    Techniques: SDS Page, Electrophoresis, Staining

    Immunoblot analysis of T. denticola ATCC 35405 (lane 1; boiled) and K1 (lane 2; boiled) with antidentilisin serum.

    Journal: Journal of Bacteriology

    Article Title: Dentilisin Activity Affects the Organization of the Outer Sheath of Treponema denticola

    doi:

    Figure Lengend Snippet: Immunoblot analysis of T. denticola ATCC 35405 (lane 1; boiled) and K1 (lane 2; boiled) with antidentilisin serum.

    Article Snippet: Zymography with gelatin as a substrate (Fig. ) indicated that a proteolytic band of approximately 100 kDa could be readily detected for wild-type strain ATCC 35405 but that this band was absent in mutant K1.

    Techniques:

    Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Journal: Infection and Immunity

    Article Title: Cytopathic Effects of the Major Surface Protein and the Chymotrypsinlike Protease of Treponema denticola

    doi:

    Figure Lengend Snippet: Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Article Snippet: The objective of the present study was to quantify and compare the adherence activity and cytotoxic effects of two important outer membrane proteins of the T. denticola type strain ATCC 35405: the pore-forming adhesin Msp, and the chymotrypsinlike protease CTLP.

    Techniques: Transmission Assay, Sonication

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Journal: Infection and Immunity

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    doi: 10.1128/IAI.70.7.3982-3984.2002

    Figure Lengend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Article Snippet: As there was little difference in hβD-2 sensitivity among strains, T. denticola ATCC 35404 was used for all experiments.

    Techniques: Incubation