trek 1 shrna lentiviral particles  (Santa Cruz Biotechnology)

 
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    Name:
    TREK 1 shRNA m Lentiviral Particles
    Description:
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of TREK 1 gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody TREK 1 Antibody F 6 sc 398449 is recommended as control antibody for monitoring of TREK 1 expression knockdown by Western blotting or immunofluorescence
    Catalog Number:
    SC-37181-V
    Price:
    None
    Category:
    Gene Editing siRNA shRNA Gene Silencers Channel Protein TREK 1 siRNA shRNA Plasmid and Lentiviral Particle Gene Silencers TREK 1 siRNA and shRNA Plasmids m
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    Structured Review

    Santa Cruz Biotechnology trek 1 shrna lentiviral particles
    <t>Trek-1</t> deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 <t>shRNA-transfected</t> cells after 48 h of culture at room air ( P
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of TREK 1 gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody TREK 1 Antibody F 6 sc 398449 is recommended as control antibody for monitoring of TREK 1 expression knockdown by Western blotting or immunofluorescence
    https://www.bioz.com/result/trek 1 shrna lentiviral particles/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trek 1 shrna lentiviral particles - by Bioz Stars, 2021-09
    85/100 stars

    Images

    1) Product Images from "Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells"

    Article Title: Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    doi: 10.1152/ajplung.00078.2011

    Trek-1 deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 shRNA-transfected cells after 48 h of culture at room air ( P
    Figure Legend Snippet: Trek-1 deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 shRNA-transfected cells after 48 h of culture at room air ( P

    Techniques Used: shRNA, Transfection

    Targeted knockdown of Trek-1 expression in shRNA-transfected MLE-12 cells. A : decreased Trek-1 expression in shRNA-treated cells in a representative Western blot and a 50% decrease in Trek-1 expression using densitometry analysis ( n = 10, * P
    Figure Legend Snippet: Targeted knockdown of Trek-1 expression in shRNA-transfected MLE-12 cells. A : decreased Trek-1 expression in shRNA-treated cells in a representative Western blot and a 50% decrease in Trek-1 expression using densitometry analysis ( n = 10, * P

    Techniques Used: Expressing, shRNA, Transfection, Western Blot

    Related Articles

    shRNA:

    Article Title: Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells
    Article Snippet: .. A stable Trek-1 knockdown cell line was developed in our laboratory using commercially available Trek-1 shRNA Lentiviral particles (cat. no. SC-37181-V; control scrambled shRNA cat. no. 108080; Santa Cruz Biotechnology, Santa Cruz, CA) following the manufacturer's instructions. ..

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  • 85
    Santa Cruz Biotechnology trek 1 shrna lentiviral particles
    <t>Trek-1</t> deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 <t>shRNA-transfected</t> cells after 48 h of culture at room air ( P
    Trek 1 Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trek 1 shrna lentiviral particles/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trek 1 shrna lentiviral particles - by Bioz Stars, 2021-09
    85/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology acute trek 1 knock down
    β IV -Spectrin is required for <t>TREK-1</t> membrane expression in ventricular cardiomyocytes. Permeabilized adult ventricular cardiomyocytes from WT and qv 4J hearts were immunostained for N-cadherin ( green ) and ( A ) TREK-1 ( red ), ( B ) TREK-2 ( red ), or ( C ) TASK-1 ( red ). The region of the intercalated disc is indicated by the white arrows . Nuclei are indicated by DAPI staining in merged images ( blue ). Scale bar = 10 µm. qv 4J ventricular cardiomyocytes show an increase in peri-nuclear localization of TREK-1 ( yellow arrow ) without any difference in TASK-1 or the homologous TREK-2.
    Acute Trek 1 Knock Down, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acute trek 1 knock down/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acute trek 1 knock down - by Bioz Stars, 2021-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Trek-1 deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 shRNA-transfected cells after 48 h of culture at room air ( P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells

    doi: 10.1152/ajplung.00078.2011

    Figure Lengend Snippet: Trek-1 deficiency increases MLE-12 cell proliferation. A : increase in absolute cell numbers in Trek-1 shRNA-transfected cells after 48 h of culture at room air ( P

    Article Snippet: A stable Trek-1 knockdown cell line was developed in our laboratory using commercially available Trek-1 shRNA Lentiviral particles (cat. no. SC-37181-V; control scrambled shRNA cat. no. 108080; Santa Cruz Biotechnology, Santa Cruz, CA) following the manufacturer's instructions.

    Techniques: shRNA, Transfection

    Targeted knockdown of Trek-1 expression in shRNA-transfected MLE-12 cells. A : decreased Trek-1 expression in shRNA-treated cells in a representative Western blot and a 50% decrease in Trek-1 expression using densitometry analysis ( n = 10, * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells

    doi: 10.1152/ajplung.00078.2011

    Figure Lengend Snippet: Targeted knockdown of Trek-1 expression in shRNA-transfected MLE-12 cells. A : decreased Trek-1 expression in shRNA-treated cells in a representative Western blot and a 50% decrease in Trek-1 expression using densitometry analysis ( n = 10, * P

    Article Snippet: A stable Trek-1 knockdown cell line was developed in our laboratory using commercially available Trek-1 shRNA Lentiviral particles (cat. no. SC-37181-V; control scrambled shRNA cat. no. 108080; Santa Cruz Biotechnology, Santa Cruz, CA) following the manufacturer's instructions.

    Techniques: Expressing, shRNA, Transfection, Western Blot

    β IV -Spectrin is required for TREK-1 membrane expression in ventricular cardiomyocytes. Permeabilized adult ventricular cardiomyocytes from WT and qv 4J hearts were immunostained for N-cadherin ( green ) and ( A ) TREK-1 ( red ), ( B ) TREK-2 ( red ), or ( C ) TASK-1 ( red ). The region of the intercalated disc is indicated by the white arrows . Nuclei are indicated by DAPI staining in merged images ( blue ). Scale bar = 10 µm. qv 4J ventricular cardiomyocytes show an increase in peri-nuclear localization of TREK-1 ( yellow arrow ) without any difference in TASK-1 or the homologous TREK-2.

    Journal: Cardiovascular Research

    Article Title: βIV-Spectrin regulates TREK-1 membrane targeting in the heart

    doi: 10.1093/cvr/cvu008

    Figure Lengend Snippet: β IV -Spectrin is required for TREK-1 membrane expression in ventricular cardiomyocytes. Permeabilized adult ventricular cardiomyocytes from WT and qv 4J hearts were immunostained for N-cadherin ( green ) and ( A ) TREK-1 ( red ), ( B ) TREK-2 ( red ), or ( C ) TASK-1 ( red ). The region of the intercalated disc is indicated by the white arrows . Nuclei are indicated by DAPI staining in merged images ( blue ). Scale bar = 10 µm. qv 4J ventricular cardiomyocytes show an increase in peri-nuclear localization of TREK-1 ( yellow arrow ) without any difference in TASK-1 or the homologous TREK-2.

    Article Snippet: Acute TREK-1 knock-down was achieved by adding shRNA lentiviral particles (Santa Cruz, 5 × 104 MOI) to each well and incubating for 0, 12, or 24 h. After incubation, cells were rinsed three times with PBS, and fixed with 95% ethanol and 5% formic acid.

    Techniques: Expressing, Staining

    β IV -Spectrin is required for TREK-1 activity in ventricular cardiomyocytes. ( A and B ) Summary data and representative current traces of arachidonic acid-sensitive current (TREK-1) in WT and qv 4J ventricular myocytes ( n = 9 from three different preparations for WT and n = 8 from two different preparations for qv 4J , * P

    Journal: Cardiovascular Research

    Article Title: βIV-Spectrin regulates TREK-1 membrane targeting in the heart

    doi: 10.1093/cvr/cvu008

    Figure Lengend Snippet: β IV -Spectrin is required for TREK-1 activity in ventricular cardiomyocytes. ( A and B ) Summary data and representative current traces of arachidonic acid-sensitive current (TREK-1) in WT and qv 4J ventricular myocytes ( n = 9 from three different preparations for WT and n = 8 from two different preparations for qv 4J , * P

    Article Snippet: Acute TREK-1 knock-down was achieved by adding shRNA lentiviral particles (Santa Cruz, 5 × 104 MOI) to each well and incubating for 0, 12, or 24 h. After incubation, cells were rinsed three times with PBS, and fixed with 95% ethanol and 5% formic acid.

    Techniques: Activity Assay