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Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast ( runx2a, runx2b, sp7/osterix, bglap and postna ) and osteoclast related genes ( ctsk ) screened at 9 dpf and 12 dpf in chd7 +/+ and chd7 -/- larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline <t>Phosphatase</t> staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7 +/+ and n = 8 for chd7 -/- ). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (F) <t>TRAP</t> staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. * p < 0.05; ** p < 0.01 *** p < 0.001; *** p < 0.0001.
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Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast ( runx2a, runx2b, sp7/osterix, bglap and postna ) and osteoclast related genes ( ctsk ) screened at 9 dpf and 12 dpf in chd7 +/+ and chd7 -/- larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline <t>Phosphatase</t> staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7 +/+ and n = 8 for chd7 -/- ). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (F) <t>TRAP</t> staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. * p < 0.05; ** p < 0.01 *** p < 0.001; *** p < 0.0001.
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Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast ( runx2a, runx2b, sp7/osterix, bglap and postna ) and osteoclast related genes ( ctsk ) screened at 9 dpf and 12 dpf in chd7 +/+ and chd7 -/- larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline <t>Phosphatase</t> staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7 +/+ and n = 8 for chd7 -/- ). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (F) <t>TRAP</t> staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. * p < 0.05; ** p < 0.01 *** p < 0.001; *** p < 0.0001.
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Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast ( runx2a, runx2b, sp7/osterix, bglap and postna ) and osteoclast related genes ( ctsk ) screened at 9 dpf and 12 dpf in chd7 +/+ and chd7 -/- larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline <t>Phosphatase</t> staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7 +/+ and n = 8 for chd7 -/- ). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (F) <t>TRAP</t> staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. * p < 0.05; ** p < 0.01 *** p < 0.001; *** p < 0.0001.
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Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast ( runx2a, runx2b, sp7/osterix, bglap and postna ) and osteoclast related genes ( ctsk ) screened at 9 dpf and 12 dpf in chd7 +/+ and chd7 -/- larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline <t>Phosphatase</t> staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7 +/+ and n = 8 for chd7 -/- ). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (F) <t>TRAP</t> staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. * p < 0.05; ** p < 0.01 *** p < 0.001; *** p < 0.0001.
Tartrate Resistant Acid Phosphatase (Trap) Enzymatic Activity Detection Kit, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast ( runx2a, runx2b, sp7/osterix, bglap and postna ) and osteoclast related genes ( ctsk ) screened at 9 dpf and 12 dpf in chd7 +/+ and chd7 -/- larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7 +/+ and n = 8 for chd7 -/- ). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (F) TRAP staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. * p < 0.05; ** p < 0.01 *** p < 0.001; *** p < 0.0001.

Journal: Journal of Bone and Mineral Research

Article Title: CHD7 regulates craniofacial cartilage development via controlling HTR2B expression

doi: 10.1093/jbmr/zjae024

Figure Lengend Snippet: Osteoblasts and Osteoclasts in early development and adults. (A) RT-qPCR of osteoblast ( runx2a, runx2b, sp7/osterix, bglap and postna ) and osteoclast related genes ( ctsk ) screened at 9 dpf and 12 dpf in chd7 +/+ and chd7 -/- larvae. (B) Immunohistochemistry of osteoblast marker Runx2 (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (C) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 200 μm. (D) Alkaline Phosphatase staining (ALP; blue) for osteoblasts in adult scales and quantitative analysis determined by signal/area ratio (n = 6 for chd7 +/+ and n = 8 for chd7 -/- ). Scale bar = 100 μm. (E) Immunohistochemistry of osteoclast marker cathepsin K (red) and nuclei maker Hoechst (blue) in 3dpf chd7 +/+ (n = 5) and chd7 -/- (n = 5) larvae. Scale bar = 60 μm. (F) TRAP staining for osteoclasts in craniofacial region of 9dpf larvae (n = 10 for both genotypes). Scale bar = 100 μm. (G) TRAP staining for osteoclasts in adult scales and quantitative analysis determined by signal/area ratio (n = 10 for both genotypes). Scale bar = 100 μm. * p < 0.05; ** p < 0.01 *** p < 0.001; *** p < 0.0001.

Article Snippet: Osteoclast activity was assessed using a leukocytes acid phosphatase tartrate-resistant acid phosphatase (TRAP) detection kit (Sigma-Aldrich, 387A) following the manufacturer’s protocol.

Techniques: Quantitative RT-PCR, Immunohistochemistry, Marker, Staining