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Corning Life Sciences transwell 6 well plates
Figure 4.  BI6727 retains pro-apoptotic effects also in the presence of a microenvironment of bone marrow stromal cells. ( A ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, than MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. The viability of treated cell lines grown alone and co-coltured was then evaluated by MTT assays. CTRL, untreated cells. ( B ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, then MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. Then, cells were separately collected, lysed, and analyzed by western blot for PARP cleavage. Molecular weights are indicated on the left. CTRL, untreated cells. ( C ) MOLT-4 cells were directly seeded on top of MS-5 cells and treated with BI6727 (40 nM). After 48 h, cells were harvested with trypsin/EDTA, washed, and resuspended in binding buffer containing Annexin V-FITC. Cells were counterstained with either a PE-conjugated anti-CD45 antibody or with an irrelevant isotypic control antibody and analyzed by flow cytometry after electronic gating on CD45. CTRL, untreated cells.
Transwell 6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Therapeutic targeting of Polo-like kinase-1 and Aurora kinases in T-cell acute lymphoblastic leukemia"

Article Title: Therapeutic targeting of Polo-like kinase-1 and Aurora kinases in T-cell acute lymphoblastic leukemia

Journal: Cell Cycle

doi: 10.4161/cc.29267

Figure 4.  BI6727 retains pro-apoptotic effects also in the presence of a microenvironment of bone marrow stromal cells. ( A ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, than MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. The viability of treated cell lines grown alone and co-coltured was then evaluated by MTT assays. CTRL, untreated cells. ( B ) The MS-5 cell line was grown in the lower chamber of Transwell ®  6-well plates, then MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. Then, cells were separately collected, lysed, and analyzed by western blot for PARP cleavage. Molecular weights are indicated on the left. CTRL, untreated cells. ( C ) MOLT-4 cells were directly seeded on top of MS-5 cells and treated with BI6727 (40 nM). After 48 h, cells were harvested with trypsin/EDTA, washed, and resuspended in binding buffer containing Annexin V-FITC. Cells were counterstained with either a PE-conjugated anti-CD45 antibody or with an irrelevant isotypic control antibody and analyzed by flow cytometry after electronic gating on CD45. CTRL, untreated cells.
Figure Legend Snippet: Figure 4. BI6727 retains pro-apoptotic effects also in the presence of a microenvironment of bone marrow stromal cells. ( A ) The MS-5 cell line was grown in the lower chamber of Transwell ® 6-well plates, than MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. The viability of treated cell lines grown alone and co-coltured was then evaluated by MTT assays. CTRL, untreated cells. ( B ) The MS-5 cell line was grown in the lower chamber of Transwell ® 6-well plates, then MOLT-4 cells were added to the upper chamber containing a 0.4-µm-polyester membrane and treated with BI6727 (40 nM) for 48 h. Then, cells were separately collected, lysed, and analyzed by western blot for PARP cleavage. Molecular weights are indicated on the left. CTRL, untreated cells. ( C ) MOLT-4 cells were directly seeded on top of MS-5 cells and treated with BI6727 (40 nM). After 48 h, cells were harvested with trypsin/EDTA, washed, and resuspended in binding buffer containing Annexin V-FITC. Cells were counterstained with either a PE-conjugated anti-CD45 antibody or with an irrelevant isotypic control antibody and analyzed by flow cytometry after electronic gating on CD45. CTRL, untreated cells.

Techniques Used: Mass Spectrometry, MTT Assay, Western Blot, Binding Assay, Flow Cytometry, Cytometry

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Centrifugation:

Article Title: Intraperitoneal cancer-immune microenvironment promotes peritoneal dissemination of gastric cancer
Article Snippet: .. Cytokine and chemokine assays TDMs (3 × 10⁵) were indirectly co-cultured with GCIY (1 × 10⁵) for 72 hours using transwell 6-well plates with 4-μm pore PET track-etched membranes (Corning), and then the cell culture supernatant mixture was collected by centrifugation. ..

Positron Emission Tomography:

Article Title: Intraperitoneal cancer-immune microenvironment promotes peritoneal dissemination of gastric cancer
Article Snippet: .. Cytokine and chemokine assays TDMs (3 × 10⁵) were indirectly co-cultured with GCIY (1 × 10⁵) for 72 hours using transwell 6-well plates with 4-μm pore PET track-etched membranes (Corning), and then the cell culture supernatant mixture was collected by centrifugation. ..

In Vitro:

Article Title: Intestinal Drug Absorption Enhancement by Aloe vera Gel and Whole Leaf Extract: In Vitro Investigations into the Mechanisms of Action
Article Snippet: .. In Vitro Permeation Studies Caco-2 cells were cultured in Transwell® 6-well plates (Corning Costar® ) on insert membranes, with a surface area of 4.67 cm2 and a pore size of 0.4 µm, to form confluent monolayers. .. For the in vitro permeation study, four different FITC-dextran (i.e., FD-4, FD-10, FD-20 and FD-40) solutions were prepared, each in a concentration of 125 μg/mL, in serum-free DMEM.

Cell Culture:

Article Title: Intestinal Drug Absorption Enhancement by Aloe vera Gel and Whole Leaf Extract: In Vitro Investigations into the Mechanisms of Action
Article Snippet: .. In Vitro Permeation Studies Caco-2 cells were cultured in Transwell® 6-well plates (Corning Costar® ) on insert membranes, with a surface area of 4.67 cm2 and a pore size of 0.4 µm, to form confluent monolayers. .. For the in vitro permeation study, four different FITC-dextran (i.e., FD-4, FD-10, FD-20 and FD-40) solutions were prepared, each in a concentration of 125 μg/mL, in serum-free DMEM.

Article Title: Human in vitro Model Reveals the Effects of Collagen Cross-linking on Keratoconus Pathogenesis
Article Snippet: .. 3-D Cell Culture and ECM Assembly HCFs and HKCs were seeded on a transwell 6-well plates with polycarbonate membrane inserts with 0.4-μm pores (Transwell; Corning Costar; Charlotte, NC) at a density of 1 × 106 cells/well and cultured in a 10% FBS EMEM medium and 1% Antibiotic, stimulated with 0.5 mM 2-O-α-D-Glucopyranosyl-L-Ascorbic Acid (Vitamin C, American Custom Chemicals Corporation, San Diego, CA). .. Corneal Collagen Cross-Linking We further developed our 3-D in vitro model to accommodate Riboflavin-UVA-CXL, establishing a setup mimicking the current clinical treatment of KC.

Article Title: Intraperitoneal cancer-immune microenvironment promotes peritoneal dissemination of gastric cancer
Article Snippet: .. Cytokine and chemokine assays TDMs (3 × 10⁵) were indirectly co-cultured with GCIY (1 × 10⁵) for 72 hours using transwell 6-well plates with 4-μm pore PET track-etched membranes (Corning), and then the cell culture supernatant mixture was collected by centrifugation. ..

Article Title: Mesenchymal stem cells cultured under hypoxic conditions had a greater therapeutic effect on mice with liver cirrhosis compared to those cultured under normal oxygen conditions
Article Snippet: .. 2.4 Co-culture of id-BMMs and norMSCs or hypoMSCs The id-BMMs were cultured with or without MSCs at 37 °C under 5% CO2 in Transwell 6-well plates (Corning) for 72 h. The id-BMMs were then harvested and their tumor necrosis factor-α (Tnfα ), Ym-1 , Fizz-1 , Cd206 , and MCP- 1 mRNA levels were compared to those of the controls. .. Prostaglandin E synthase (Ptges ) from hypoMSCs was knocked down with HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

Article Title: The garlic compound ajoene covalently binds vimentin, disrupts the vimentin network and exerts anti-metastatic activity in cancer cells
Article Snippet: .. Transwell 6-well plates with permeable cell culture inserts of 12 mm diameter with 8 μm pores (Corning) were also chilled to 4 °C. ..

Article Title: Collagen cross-linking impact on keratoconus extracellular matrix
Article Snippet: .. Briefly, HCFs and HKCs were seeded on transwell 6-well plates with polycarbonate membrane inserts with 0.4-μm pores (Transwell; Corning Costar; Charlotte, NC) at a density of 1 × 106 cells/well and cultured in 1.5 mL of 10% FBS EMEM medium and 1% Antibiotic, stimulated with 0.5 mM 2-O-α-D-Glucopyranosyl-L-Ascorbic Acid (Vitamin C, American Custom Chemicals Corporation, San Diego, CA) in both top and bottom wells. ..

Co-Culture Assay:

Article Title: Mesenchymal stem cells cultured under hypoxic conditions had a greater therapeutic effect on mice with liver cirrhosis compared to those cultured under normal oxygen conditions
Article Snippet: .. 2.4 Co-culture of id-BMMs and norMSCs or hypoMSCs The id-BMMs were cultured with or without MSCs at 37 °C under 5% CO2 in Transwell 6-well plates (Corning) for 72 h. The id-BMMs were then harvested and their tumor necrosis factor-α (Tnfα ), Ym-1 , Fizz-1 , Cd206 , and MCP- 1 mRNA levels were compared to those of the controls. .. Prostaglandin E synthase (Ptges ) from hypoMSCs was knocked down with HiPerFect Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.

Mass Spectrometry:

Article Title: Therapeutic targeting of Polo-like kinase-1 and Aurora kinases in T-cell acute lymphoblastic leukemia
Article Snippet: .. In another set of experiments, MS-5 cells were grown in the lower chamber of Transwell® 6-well plates (Corning) containing a 0.4-µm polyester membrane, then MOLT-4 cells were added to the upper chamber and treated with BI6727 (40 nM). ..

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  • 92
    Corning Life Sciences transwell inserts
    DC-SIGNR promotes colon cancer cell migration and invasion in vitro. a Crystal violet staining showed that DC-SIGNR positively promoted the migration and invasion ability of the three colon cancer cell lines. Scale bars , 100 μm. b The in vitro migration and invasion promoting activities of colon cancer cells treated with the DC-SIGNR protein or control IgG were measured by determining the number of cells that passed through the <t>Transwell</t> membrane. c Morphology micrographs of the three types of colon cancer cells treated with the DC-SIGNR protein or control IgG for 24 h. The DC-SIGNR protein induced scattering of the three types of colon cancer cells. Scale bars, 50 μm. d Pictures of the wound healing assay in LoVo cells incubated with the DC-SIGNR protein or control IgG at 24 h after the cells were scratched. The LoVo cells treated with the DC-SIGNR protein migrated more quickly than those treated with control IgG. Scale bars , 100 μm. e The relative cell migrations of the three colon cancer cell lines at 24 h after the cells were scratched are shown in this panel. The error bars in all graphs represent SD, and the results were quantified from three experiments
    Transwell Inserts, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transwell inserts/product/Corning Life Sciences
    Average 92 stars, based on 335 article reviews
    Price from $9.99 to $1999.99
    transwell inserts - by Bioz Stars, 2020-09
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    88
    Corning Life Sciences polyester transwell membranes
    Transendothelial electrical resistance on successive days after seeding (Day 0) of PBMEC on <t>transwell</t> system. TEER is expressed as total resistance – blank (cell free) resistance. Total resistance is 180–200 ± 8 ohms, blank resistance is 102±2 ohms. The arrow indicates the addition of culture medium supplemented with 250 μM CPT-cAMP and 17.5 μM of RO-20-1724. Values represent the mean of three measurements
    Polyester Transwell Membranes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 9 article reviews
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    89
    Corning Life Sciences 12 well transwell plates
    Evaluation of the BBB-penetration ability of CPDs in vitro. (A–C) Schematic diagram of the preparation and application of the in vitro BBB model: (A) the <t>12-well</t> plates were seeded with C6 cells; (B) HUVEC were seeded into the <t>transwell</t> inserts; (C) CPDs were added into the transwell lumen, and the fluid in the lower compartment containing the CPDs could be detected. (D) Time-dependent TEER values of the in vitro BBB model ( n = 3, mean ± SD). (E) Accumulated percentages of CPDs that crossed the BBB ( n = 6, mean ± SD).
    12 Well Transwell Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DC-SIGNR promotes colon cancer cell migration and invasion in vitro. a Crystal violet staining showed that DC-SIGNR positively promoted the migration and invasion ability of the three colon cancer cell lines. Scale bars , 100 μm. b The in vitro migration and invasion promoting activities of colon cancer cells treated with the DC-SIGNR protein or control IgG were measured by determining the number of cells that passed through the Transwell membrane. c Morphology micrographs of the three types of colon cancer cells treated with the DC-SIGNR protein or control IgG for 24 h. The DC-SIGNR protein induced scattering of the three types of colon cancer cells. Scale bars, 50 μm. d Pictures of the wound healing assay in LoVo cells incubated with the DC-SIGNR protein or control IgG at 24 h after the cells were scratched. The LoVo cells treated with the DC-SIGNR protein migrated more quickly than those treated with control IgG. Scale bars , 100 μm. e The relative cell migrations of the three colon cancer cell lines at 24 h after the cells were scratched are shown in this panel. The error bars in all graphs represent SD, and the results were quantified from three experiments

    Journal: Journal of Hematology & Oncology

    Article Title: Novel roles of DC-SIGNR in colon cancer cell adhesion, migration, invasion, and liver metastasis

    doi: 10.1186/s13045-016-0383-x

    Figure Lengend Snippet: DC-SIGNR promotes colon cancer cell migration and invasion in vitro. a Crystal violet staining showed that DC-SIGNR positively promoted the migration and invasion ability of the three colon cancer cell lines. Scale bars , 100 μm. b The in vitro migration and invasion promoting activities of colon cancer cells treated with the DC-SIGNR protein or control IgG were measured by determining the number of cells that passed through the Transwell membrane. c Morphology micrographs of the three types of colon cancer cells treated with the DC-SIGNR protein or control IgG for 24 h. The DC-SIGNR protein induced scattering of the three types of colon cancer cells. Scale bars, 50 μm. d Pictures of the wound healing assay in LoVo cells incubated with the DC-SIGNR protein or control IgG at 24 h after the cells were scratched. The LoVo cells treated with the DC-SIGNR protein migrated more quickly than those treated with control IgG. Scale bars , 100 μm. e The relative cell migrations of the three colon cancer cell lines at 24 h after the cells were scratched are shown in this panel. The error bars in all graphs represent SD, and the results were quantified from three experiments

    Article Snippet: The in vitro migration assay was performed in 24-well plates with Transwell inserts (6.5 mm in diameter, 8-μm pore size, Corning, USA).

    Techniques: Migration, In Vitro, Staining, Wound Healing Assay, Incubation

    Migration of transduced hCXCR4 positive (hCXCR4+) or negative (hCXCR4−) MSCs through transwells toward SDF-1α positive (SDF-1α+) or negative (SDF-1α−) medium from transduced fat tissue grafts ( n = 6 each group). A comparison of all groups showed statistically significant differences ( p

    Journal: Journal of biomedical materials research. Part A

    Article Title: Stem cell attraction via SDF-1? expressing fat tissue grafts

    doi: 10.1002/jbm.a.34512

    Figure Lengend Snippet: Migration of transduced hCXCR4 positive (hCXCR4+) or negative (hCXCR4−) MSCs through transwells toward SDF-1α positive (SDF-1α+) or negative (SDF-1α−) medium from transduced fat tissue grafts ( n = 6 each group). A comparison of all groups showed statistically significant differences ( p

    Article Snippet: The migration experiment was done using transwells (24-well plates, pore size 8.0 um, 6.5 mm insert, Corning).

    Techniques: Migration

    Transendothelial electrical resistance on successive days after seeding (Day 0) of PBMEC on transwell system. TEER is expressed as total resistance – blank (cell free) resistance. Total resistance is 180–200 ± 8 ohms, blank resistance is 102±2 ohms. The arrow indicates the addition of culture medium supplemented with 250 μM CPT-cAMP and 17.5 μM of RO-20-1724. Values represent the mean of three measurements

    Journal: Experimental brain research

    Article Title: Endomorphins exit the brain by a saturable efflux system at the basolateral surface of cerebral endothelial cells

    doi: 10.1007/s00221-003-1774-0

    Figure Lengend Snippet: Transendothelial electrical resistance on successive days after seeding (Day 0) of PBMEC on transwell system. TEER is expressed as total resistance – blank (cell free) resistance. Total resistance is 180–200 ± 8 ohms, blank resistance is 102±2 ohms. The arrow indicates the addition of culture medium supplemented with 250 μM CPT-cAMP and 17.5 μM of RO-20-1724. Values represent the mean of three measurements

    Article Snippet: The cells were applied to polyester transwell membranes (6.5 mm diameter, 0.4 μm pore size in 24 well plates; Corning, Corning, NY, USA), which were previously coated with 50 μg/ml rat tail collagen type I.

    Techniques: Cycling Probe Technology

    Transport of 125 I-EM-1 and 125 I-lactalbumin from the basolateral to apical direction on mouse endothelial cells grown on collagen-coated transwell. In some experiments, unlabeled EM-1 or EM-2 (100 ng/ml or 1,000 ng/ml) was added to the transport buffer. This resulted in significant self-inhibition characteristic of a saturable system. Each data point represents the mean ± SEM of three determinations

    Journal: Experimental brain research

    Article Title: Endomorphins exit the brain by a saturable efflux system at the basolateral surface of cerebral endothelial cells

    doi: 10.1007/s00221-003-1774-0

    Figure Lengend Snippet: Transport of 125 I-EM-1 and 125 I-lactalbumin from the basolateral to apical direction on mouse endothelial cells grown on collagen-coated transwell. In some experiments, unlabeled EM-1 or EM-2 (100 ng/ml or 1,000 ng/ml) was added to the transport buffer. This resulted in significant self-inhibition characteristic of a saturable system. Each data point represents the mean ± SEM of three determinations

    Article Snippet: The cells were applied to polyester transwell membranes (6.5 mm diameter, 0.4 μm pore size in 24 well plates; Corning, Corning, NY, USA), which were previously coated with 50 μg/ml rat tail collagen type I.

    Techniques: Inhibition

    Transport of 125 I-EM-2 and 125 I-lactalbumin from the basolateral to apical side in mouse endothelial cells grown on collagen-coated transwell. In some experiments, unlabeled EM-1 or EM-2 (100 ng/ml or 1,000 ng/ml) was added to the transport buffer. This resulted in self-inhibition characteristic of a saturable system. P

    Journal: Experimental brain research

    Article Title: Endomorphins exit the brain by a saturable efflux system at the basolateral surface of cerebral endothelial cells

    doi: 10.1007/s00221-003-1774-0

    Figure Lengend Snippet: Transport of 125 I-EM-2 and 125 I-lactalbumin from the basolateral to apical side in mouse endothelial cells grown on collagen-coated transwell. In some experiments, unlabeled EM-1 or EM-2 (100 ng/ml or 1,000 ng/ml) was added to the transport buffer. This resulted in self-inhibition characteristic of a saturable system. P

    Article Snippet: The cells were applied to polyester transwell membranes (6.5 mm diameter, 0.4 μm pore size in 24 well plates; Corning, Corning, NY, USA), which were previously coated with 50 μg/ml rat tail collagen type I.

    Techniques: Inhibition

    Evaluation of the BBB-penetration ability of CPDs in vitro. (A–C) Schematic diagram of the preparation and application of the in vitro BBB model: (A) the 12-well plates were seeded with C6 cells; (B) HUVEC were seeded into the transwell inserts; (C) CPDs were added into the transwell lumen, and the fluid in the lower compartment containing the CPDs could be detected. (D) Time-dependent TEER values of the in vitro BBB model ( n = 3, mean ± SD). (E) Accumulated percentages of CPDs that crossed the BBB ( n = 6, mean ± SD).

    Journal: ACS Omega

    Article Title: Noninvasive Brain Tumor Imaging Using Red Emissive Carbonized Polymer Dots across the Blood–Brain Barrier

    doi: 10.1021/acsomega.8b01169

    Figure Lengend Snippet: Evaluation of the BBB-penetration ability of CPDs in vitro. (A–C) Schematic diagram of the preparation and application of the in vitro BBB model: (A) the 12-well plates were seeded with C6 cells; (B) HUVEC were seeded into the transwell inserts; (C) CPDs were added into the transwell lumen, and the fluid in the lower compartment containing the CPDs could be detected. (D) Time-dependent TEER values of the in vitro BBB model ( n = 3, mean ± SD). (E) Accumulated percentages of CPDs that crossed the BBB ( n = 6, mean ± SD).

    Article Snippet: First, the polycarbonate membranes of 12-well transwell plates (Corning Incorporated) were coated with rat-tail type I collagen before use.

    Techniques: In Vitro