Transfections Baby Hamster Kidney Bhk 21 Cells, supplied by Boster Bio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge"
Article Title: Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge
Journal: PLoS ONE
Figure Legend Snippet: Detection of the FMDV structure protein and FMDV capsid. Immunofluoresence was used to determine the expression levels of the FMDV structure protein following transfection with either pcDNA3.1(+) (a), pA (b) pB (c) or pC (d). FMDV capsid was observed by TEM of BHK-21 cells transfected with pA (e) or pC (d).
Techniques Used: Expressing, Transfection, Transmission Electron Microscopy
Figure Legend Snippet: Characterization of FMDV protein and IL-6 production in BHK-21 cells. (A)Two days after transfection with either pA, pB or pC, and negative control (PBS) and irrelevant control (Bovine Serum Albumin, BSA) were added; BHK-21 cells were analyzed for expression of FMDV proteins by sandwich-ELISA. BHK-21 cell lysates were diluted twofold. The data are expressed as the mean OD for each dilution. (B) Expression of IL-6 was determined by assessing IL-6 levels in BHK-21 cell lysates by ELISA two-days after transfection. The data are expressed as the mean OD ± SEM, measured in duplicate. Means were compared by non-parametric ANOVA.
Techniques Used: Transfection, Negative Control, Expressing, Sandwich ELISA, Enzyme-linked Immunosorbent Assay