transfections baby hamster kidney bhk 21 cells  (Boster Bio)

 
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    Boster Bio transfections baby hamster kidney bhk 21 cells
    Detection of the FMDV structure protein and FMDV capsid. Immunofluoresence was used to determine the expression levels of the FMDV structure protein following <t>transfection</t> with either pcDNA3.1(+) (a), pA (b) pB (c) or pC (d). FMDV capsid was observed by TEM of <t>BHK-21</t> cells transfected with pA (e) or pC (d).
    Transfections Baby Hamster Kidney Bhk 21 Cells, supplied by Boster Bio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfections baby hamster kidney bhk 21 cells/product/Boster Bio
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transfections baby hamster kidney bhk 21 cells - by Bioz Stars, 2020-09
    85/100 stars

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    1) Product Images from "Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge"

    Article Title: Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0027605

    Detection of the FMDV structure protein and FMDV capsid. Immunofluoresence was used to determine the expression levels of the FMDV structure protein following transfection with either pcDNA3.1(+) (a), pA (b) pB (c) or pC (d). FMDV capsid was observed by TEM of BHK-21 cells transfected with pA (e) or pC (d).
    Figure Legend Snippet: Detection of the FMDV structure protein and FMDV capsid. Immunofluoresence was used to determine the expression levels of the FMDV structure protein following transfection with either pcDNA3.1(+) (a), pA (b) pB (c) or pC (d). FMDV capsid was observed by TEM of BHK-21 cells transfected with pA (e) or pC (d).

    Techniques Used: Expressing, Transfection, Transmission Electron Microscopy

    Characterization of FMDV protein and IL-6 production in BHK-21 cells. (A)Two days after transfection with either pA, pB or pC, and negative control (PBS) and irrelevant control (Bovine Serum Albumin, BSA) were added; BHK-21 cells were analyzed for expression of FMDV proteins by sandwich-ELISA. BHK-21 cell lysates were diluted twofold. The data are expressed as the mean OD for each dilution. (B) Expression of IL-6 was determined by assessing IL-6 levels in BHK-21 cell lysates by ELISA two-days after transfection. The data are expressed as the mean OD ± SEM, measured in duplicate. Means were compared by non-parametric ANOVA.
    Figure Legend Snippet: Characterization of FMDV protein and IL-6 production in BHK-21 cells. (A)Two days after transfection with either pA, pB or pC, and negative control (PBS) and irrelevant control (Bovine Serum Albumin, BSA) were added; BHK-21 cells were analyzed for expression of FMDV proteins by sandwich-ELISA. BHK-21 cell lysates were diluted twofold. The data are expressed as the mean OD for each dilution. (B) Expression of IL-6 was determined by assessing IL-6 levels in BHK-21 cell lysates by ELISA two-days after transfection. The data are expressed as the mean OD ± SEM, measured in duplicate. Means were compared by non-parametric ANOVA.

    Techniques Used: Transfection, Negative Control, Expressing, Sandwich ELISA, Enzyme-linked Immunosorbent Assay

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    Article Snippet: Transfections Baby hamster kidney (BHK-21) cells (Boster, Wuhan, China) (4×105 ) were seeded onto cover slips on six-well plates and incubated at 37°C in a CO2 incubator until the cells were 80% confluent.

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    Boster Bio transfections baby hamster kidney bhk 21 cells
    Detection of the FMDV structure protein and FMDV capsid. Immunofluoresence was used to determine the expression levels of the FMDV structure protein following <t>transfection</t> with either pcDNA3.1(+) (a), pA (b) pB (c) or pC (d). FMDV capsid was observed by TEM of <t>BHK-21</t> cells transfected with pA (e) or pC (d).
    Transfections Baby Hamster Kidney Bhk 21 Cells, supplied by Boster Bio, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfections baby hamster kidney bhk 21 cells/product/Boster Bio
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transfections baby hamster kidney bhk 21 cells - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Detection of the FMDV structure protein and FMDV capsid. Immunofluoresence was used to determine the expression levels of the FMDV structure protein following transfection with either pcDNA3.1(+) (a), pA (b) pB (c) or pC (d). FMDV capsid was observed by TEM of BHK-21 cells transfected with pA (e) or pC (d).

    Journal: PLoS ONE

    Article Title: Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge

    doi: 10.1371/journal.pone.0027605

    Figure Lengend Snippet: Detection of the FMDV structure protein and FMDV capsid. Immunofluoresence was used to determine the expression levels of the FMDV structure protein following transfection with either pcDNA3.1(+) (a), pA (b) pB (c) or pC (d). FMDV capsid was observed by TEM of BHK-21 cells transfected with pA (e) or pC (d).

    Article Snippet: Transfections Baby hamster kidney (BHK-21) cells (Boster, Wuhan, China) (4×105 ) were seeded onto cover slips on six-well plates and incubated at 37°C in a CO2 incubator until the cells were 80% confluent.

    Techniques: Expressing, Transfection, Transmission Electron Microscopy

    Characterization of FMDV protein and IL-6 production in BHK-21 cells. (A)Two days after transfection with either pA, pB or pC, and negative control (PBS) and irrelevant control (Bovine Serum Albumin, BSA) were added; BHK-21 cells were analyzed for expression of FMDV proteins by sandwich-ELISA. BHK-21 cell lysates were diluted twofold. The data are expressed as the mean OD for each dilution. (B) Expression of IL-6 was determined by assessing IL-6 levels in BHK-21 cell lysates by ELISA two-days after transfection. The data are expressed as the mean OD ± SEM, measured in duplicate. Means were compared by non-parametric ANOVA.

    Journal: PLoS ONE

    Article Title: Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge

    doi: 10.1371/journal.pone.0027605

    Figure Lengend Snippet: Characterization of FMDV protein and IL-6 production in BHK-21 cells. (A)Two days after transfection with either pA, pB or pC, and negative control (PBS) and irrelevant control (Bovine Serum Albumin, BSA) were added; BHK-21 cells were analyzed for expression of FMDV proteins by sandwich-ELISA. BHK-21 cell lysates were diluted twofold. The data are expressed as the mean OD for each dilution. (B) Expression of IL-6 was determined by assessing IL-6 levels in BHK-21 cell lysates by ELISA two-days after transfection. The data are expressed as the mean OD ± SEM, measured in duplicate. Means were compared by non-parametric ANOVA.

    Article Snippet: Transfections Baby hamster kidney (BHK-21) cells (Boster, Wuhan, China) (4×105 ) were seeded onto cover slips on six-well plates and incubated at 37°C in a CO2 incubator until the cells were 80% confluent.

    Techniques: Transfection, Negative Control, Expressing, Sandwich ELISA, Enzyme-linked Immunosorbent Assay