transfection reagent  (Bio-Rad)

 
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    Name:
    TransFectin Lipid Reagent
    Description:
    0 5 ml 1 5 mg ml lipid transfection reagent for a variety of applications including plasmid DNA siRNA and shRNA delivery
    Catalog Number:
    1703350
    Price:
    None
    Category:
    Lipid Transfection
    Buy from Supplier


    Structured Review

    Bio-Rad transfection reagent
    TransFectin Lipid Reagent
    0 5 ml 1 5 mg ml lipid transfection reagent for a variety of applications including plasmid DNA siRNA and shRNA delivery
    https://www.bioz.com/result/transfection reagent/product/Bio-Rad
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    transfection reagent - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro"

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro

    Journal: BMC Medical Genetics

    doi: 10.1186/1471-2350-8-65

    Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A
    Figure Legend Snippet: Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A", B" and C") are shown. In HL-1 cells immunostaining with monoclonal desmoglein antibody DG 3.10 showed both the presence of well-assembled desmosomes (panel D', E' and F') and the reduced co-localisation between endogenous dsg and mutated DSC2 (yellow dots in panel E", F").

    Techniques Used: Transfection, Cell Culture, Immunostaining, Staining

    2) Product Images from "The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation"

    Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34422-7

    Inhibition of ribosomal assembly by the CRE. ( a ) Diagram of the HCV transcripts harbouring the IRES and the CRE regions used in the polysome profiling assays. ( b ) The CRE region hinders ribosome association with the subgenomic HCV RNA transcripts. Huh-7 cells were transfected with the different RNA constructs shown in ( a ). At 4 h post-transfection, cell lysates were subjected to polysome separation followed by RT-qPCR for quantification of the viral subgenomic transcripts. The histogram represents relative HCV RNA levels normalised to those obtained for the cellular GAPDH mRNA. NPF, non-polysomal fractions; PF, polysomal fractions.
    Figure Legend Snippet: Inhibition of ribosomal assembly by the CRE. ( a ) Diagram of the HCV transcripts harbouring the IRES and the CRE regions used in the polysome profiling assays. ( b ) The CRE region hinders ribosome association with the subgenomic HCV RNA transcripts. Huh-7 cells were transfected with the different RNA constructs shown in ( a ). At 4 h post-transfection, cell lysates were subjected to polysome separation followed by RT-qPCR for quantification of the viral subgenomic transcripts. The histogram represents relative HCV RNA levels normalised to those obtained for the cellular GAPDH mRNA. NPF, non-polysomal fractions; PF, polysomal fractions.

    Techniques Used: Inhibition, Transfection, Construct, Quantitative RT-PCR

    Related Articles

    Electroporation:

    Article Title: Comparative Analysis of Non-viral Transfection Methods in Mouse Embryonic Fibroblast Cells
    Article Snippet: .. In this study, we compared the transfection efficiency of several non-viral transfection reagents, including lipid based [Lipofectamine LTX (Thermo Fisher Scientific, Waltham, MA, USA), TransFectin (Bio-Rad Laboratories, Hercules, CA, USA), GenJet In Vitro DNA Transfection Reagent (Ver II; SignaGen Laboratories, Rockville, MD, USA), DreamFect Gold (OZ Biosciences, San Diego, CA, USA)], dendrimer based [NanoJuice (EMD Millipore, Billerica, MA, USA)], polyamine based [GeneJuice (EMD Millipore)], and electroporation. .. We also tested the effect of magnetofection [CombiMag (OZ Biosciences)] on the transfection efficiency of the lipid reagents.

    Transfection:

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation
    Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of transfection reagent (TransFectinTM ; Bio-Rad) and added to cell cultures. ..

    Article Title: Comparative Analysis of Non-viral Transfection Methods in Mouse Embryonic Fibroblast Cells
    Article Snippet: .. In this study, we compared the transfection efficiency of several non-viral transfection reagents, including lipid based [Lipofectamine LTX (Thermo Fisher Scientific, Waltham, MA, USA), TransFectin (Bio-Rad Laboratories, Hercules, CA, USA), GenJet In Vitro DNA Transfection Reagent (Ver II; SignaGen Laboratories, Rockville, MD, USA), DreamFect Gold (OZ Biosciences, San Diego, CA, USA)], dendrimer based [NanoJuice (EMD Millipore, Billerica, MA, USA)], polyamine based [GeneJuice (EMD Millipore)], and electroporation. .. We also tested the effect of magnetofection [CombiMag (OZ Biosciences)] on the transfection efficiency of the lipid reagents.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

    Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells
    Article Snippet: .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the TransFectin lipid reagent (Bio-Rad Laboratories, Hercules, CA, USA). .. Lipofectamine 3000, or 1.0 mg/mL polyethyleneimine "Max" (PEI-Max) (Polysciences, Inc., Warrington, PA, USA) according to the manufacturer's instructions.

    Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
    Article Snippet: .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    Luciferase:

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    In Vitro:

    Article Title: Comparative Analysis of Non-viral Transfection Methods in Mouse Embryonic Fibroblast Cells
    Article Snippet: .. In this study, we compared the transfection efficiency of several non-viral transfection reagents, including lipid based [Lipofectamine LTX (Thermo Fisher Scientific, Waltham, MA, USA), TransFectin (Bio-Rad Laboratories, Hercules, CA, USA), GenJet In Vitro DNA Transfection Reagent (Ver II; SignaGen Laboratories, Rockville, MD, USA), DreamFect Gold (OZ Biosciences, San Diego, CA, USA)], dendrimer based [NanoJuice (EMD Millipore, Billerica, MA, USA)], polyamine based [GeneJuice (EMD Millipore)], and electroporation. .. We also tested the effect of magnetofection [CombiMag (OZ Biosciences)] on the transfection efficiency of the lipid reagents.

    Mutagenesis:

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    Construct:

    Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation
    Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of transfection reagent (TransFectinTM ; Bio-Rad) and added to cell cultures. ..

    Article Title: Structure and function analysis of the essential 3′X domain of hepatitis C virus
    Article Snippet: .. A mixture containing 1 µg of wild-type or 3′X mutant ICU RNA construct containing firefly luciferase mRNA flanked by the HCV genomic ends, and 0.25 µg of cap-dependent RLuc-mRNA, was used for cell transfection with TransFectin lipid reagent (Bio-Rad). .. Translational efficiency was determined by measuring firefly and Renilla luciferase activities using the Dual-Luciferase Reporter Assay System (Promega).

    Transient Transfection Assay:

    Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
    Article Snippet: .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

    Plasmid Preparation:

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
    Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

    Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells
    Article Snippet: .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the TransFectin lipid reagent (Bio-Rad Laboratories, Hercules, CA, USA). .. Lipofectamine 3000, or 1.0 mg/mL polyethyleneimine "Max" (PEI-Max) (Polysciences, Inc., Warrington, PA, USA) according to the manufacturer's instructions.

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
    Article Snippet: .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

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  • 94
    Bio-Rad transfection reagents
    siRNA-mediated PKCδ knockdown blocks PMA-induced NHE2 promoter activity. C2BBe1 cells were transiently transfected with PKCδ siRNA before <t>transfection</t> with p-415/+150. Cells were incubated in serum-reduced media for 16 h before PMA treatment. Cells were either treated with 100 nM PMA or vehicle. Cells were collected 48 h after transfection, and luciferase activities were determined in 10 μg of total cell proteins. The results are presented as average of 3 independent experiments performed in triplicate ( n = 3). Asterisk indicates differences between luciferase activities of the PMA-treated control siRNA transfected cells vs. similarly treated cells transfected with PKCδ siRNA, P
    Transfection Reagents, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection reagents/product/Bio-Rad
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    transfection reagents - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    85
    Bio-Rad silenfect biorad transfection reagent
    Functional effects of knocking out MSN in three glioblastoma and one control cell line . Four MSN targeting siRNAs at a final concentration of 13 nM were transfected with <t>Silenfect</t> (BioRad) <t>transfection</t> reagent to A172, LN405 and U87MG glioma cell lines and the SVGp12 control cell line. (a) Cell proliferation was assayed 72 h after transfection using CellTiter-Glo Cell Viability assay. (b) Induction of caspase-3 and -7 activities was detected 48 h after transfection with homogeneous Apo-ONE assay (Promega). Loess normalized signals from the proliferation and caspase-3/7 assays are presented as relative scores to the mean of lipid-containing wells. Significant P -values
    Silenfect Biorad Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/silenfect biorad transfection reagent/product/Bio-Rad
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    silenfect biorad transfection reagent - by Bioz Stars, 2020-11
    85/100 stars
      Buy from Supplier

    99
    Bio-Rad transfection reagent
    <t>Transfection</t> studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A
    Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection reagent/product/Bio-Rad
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    transfection reagent - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    siRNA-mediated PKCδ knockdown blocks PMA-induced NHE2 promoter activity. C2BBe1 cells were transiently transfected with PKCδ siRNA before transfection with p-415/+150. Cells were incubated in serum-reduced media for 16 h before PMA treatment. Cells were either treated with 100 nM PMA or vehicle. Cells were collected 48 h after transfection, and luciferase activities were determined in 10 μg of total cell proteins. The results are presented as average of 3 independent experiments performed in triplicate ( n = 3). Asterisk indicates differences between luciferase activities of the PMA-treated control siRNA transfected cells vs. similarly treated cells transfected with PKCδ siRNA, P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: PKC?-dependent activation of ERK1/2 leads to upregulation of the human NHE2 transcriptional activity in intestinal epithelial cell line C2BBe1

    doi: 10.1152/ajpgi.00363.2011

    Figure Lengend Snippet: siRNA-mediated PKCδ knockdown blocks PMA-induced NHE2 promoter activity. C2BBe1 cells were transiently transfected with PKCδ siRNA before transfection with p-415/+150. Cells were incubated in serum-reduced media for 16 h before PMA treatment. Cells were either treated with 100 nM PMA or vehicle. Cells were collected 48 h after transfection, and luciferase activities were determined in 10 μg of total cell proteins. The results are presented as average of 3 independent experiments performed in triplicate ( n = 3). Asterisk indicates differences between luciferase activities of the PMA-treated control siRNA transfected cells vs. similarly treated cells transfected with PKCδ siRNA, P

    Article Snippet: For small RNA interference studies, C2BBe1 cells were transfected with PKCδ-specific siRNA (20 nM) and control nontargeting siRNA (20 nM) (Santa Cruz Biotechnology) using transfection reagents from Bio-Rad as recommended by the manufacturer.

    Techniques: Activity Assay, Transfection, Incubation, Luciferase

    Functional effects of knocking out MSN in three glioblastoma and one control cell line . Four MSN targeting siRNAs at a final concentration of 13 nM were transfected with Silenfect (BioRad) transfection reagent to A172, LN405 and U87MG glioma cell lines and the SVGp12 control cell line. (a) Cell proliferation was assayed 72 h after transfection using CellTiter-Glo Cell Viability assay. (b) Induction of caspase-3 and -7 activities was detected 48 h after transfection with homogeneous Apo-ONE assay (Promega). Loess normalized signals from the proliferation and caspase-3/7 assays are presented as relative scores to the mean of lipid-containing wells. Significant P -values

    Journal: Genome Medicine

    Article Title: Large-scale data integration framework provides a comprehensive view on glioblastoma multiforme

    doi: 10.1186/gm186

    Figure Lengend Snippet: Functional effects of knocking out MSN in three glioblastoma and one control cell line . Four MSN targeting siRNAs at a final concentration of 13 nM were transfected with Silenfect (BioRad) transfection reagent to A172, LN405 and U87MG glioma cell lines and the SVGp12 control cell line. (a) Cell proliferation was assayed 72 h after transfection using CellTiter-Glo Cell Viability assay. (b) Induction of caspase-3 and -7 activities was detected 48 h after transfection with homogeneous Apo-ONE assay (Promega). Loess normalized signals from the proliferation and caspase-3/7 assays are presented as relative scores to the mean of lipid-containing wells. Significant P -values

    Article Snippet: Control siRNAs (13 nM final concentration) were transfected with Silenfect (BioRad) transfection reagent to A172, LN405 and U87MG glioma cell lines and the SVGp12 control cell line.

    Techniques: Functional Assay, Concentration Assay, Transfection, Viability Assay

    Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A

    Journal: BMC Medical Genetics

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro

    doi: 10.1186/1471-2350-8-65

    Figure Lengend Snippet: Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A", B" and C") are shown. In HL-1 cells immunostaining with monoclonal desmoglein antibody DG 3.10 showed both the presence of well-assembled desmosomes (panel D', E' and F') and the reduced co-localisation between endogenous dsg and mutated DSC2 (yellow dots in panel E", F").

    Article Snippet: Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad).

    Techniques: Transfection, Cell Culture, Immunostaining, Staining