transfectin transfection reagent (Bio-Rad)
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Transfectin Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Transfection:Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential Article Snippet: The primers of TWIST1 for vector construction is shown in . .. Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and Article Title: Targeted correction of a thalassemia-associated ?-globin mutation induced by pseudo-complementary peptide nucleic acids Article Snippet: After incubation to allow plasmid:pcPNA complex formation, the samples were digested with PvuII to release a 400 bp fragment, and binding was assayed by gel mobility shift as described above. .. Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells Article Snippet: CYP expression in 293FT cells 293FT cells purchased from ThermoFisher Scientific were cultured in Dulbecco's modified Eagle's medium (Nacalai Tesque) containing 10% foetal bovine serum at 37 °C under 5% CO2 . .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro Article Snippet: Cellquest software (Version 5.1, Becton Dickinson, Franklin Lakes, NJ, USA) was used to determine the cell cycle profiles. .. Transient Transfection Assay The Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers Article Snippet: Studies of gene transfection in the primary neurons have mainly been based on fetal primary neuron culture and data on gene transfer to the adult cortical neurons are scarce. .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by Mutagenesis:Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of Plasmid Preparation:Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential Article Snippet: The primers of TWIST1 for vector construction is shown in . .. Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells Article Snippet: CYP expression in 293FT cells 293FT cells purchased from ThermoFisher Scientific were cultured in Dulbecco's modified Eagle's medium (Nacalai Tesque) containing 10% foetal bovine serum at 37 °C under 5% CO2 . .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers Article Snippet: Studies of gene transfection in the primary neurons have mainly been based on fetal primary neuron culture and data on gene transfer to the adult cortical neurons are scarce. .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by Construct:Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of Expressing:Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential Article Snippet: The primers of TWIST1 for vector construction is shown in . .. Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and Transient Transfection Assay:Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro Article Snippet: Cellquest software (Version 5.1, Becton Dickinson, Franklin Lakes, NJ, USA) was used to determine the cell cycle profiles. .. Transient Transfection Assay The |