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Bio-Rad transfectin transfection reagent
Transfectin Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transfectin transfection reagent/product/Bio-Rad
Average 96 stars, based on 1 article reviews
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transfectin transfection reagent - by Bioz Stars, 2021-03
96/100 stars

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Transfection:

Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation
Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of transfection reagent (TransFectinTM ; Bio-Rad) and added to cell cultures. ..

Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential
Article Snippet: The primers of TWIST1 for vector construction is shown in . .. Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and transfection process was performed by Gene Pulser X-cell (Bio-Rad) according to Gene Pulser Electroprotocal. .. Twenty-four to 72 hours after transfection, cells were trypsinized and collected for RNA purification or protein sample preparation.

Article Title: Targeted correction of a thalassemia-associated ?-globin mutation induced by pseudo-complementary peptide nucleic acids
Article Snippet: After incubation to allow plasmid:pcPNA complex formation, the samples were digested with PvuII to release a 400 bp fragment, and binding was assayed by gel mobility shift as described above. .. Transfection Total 1 × 106 cells in 100 μl of media were mixed with selected pcPNAs (6 μM) and GFP-DNA (12 μM) oligonucleotides and electroporated in 0.4 cm cuvettes using a Bio-Rad Gene Pulser (280 V, 960 μFd, Hercules, CA). .. Cells were replated in 60 mm dishes following electroporation and allowed to expand to ∼90% confluency (2–3 days).

Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells
Article Snippet: CYP expression in 293FT cells 293FT cells purchased from ThermoFisher Scientific were cultured in Dulbecco's modified Eagle's medium (Nacalai Tesque) containing 10% foetal bovine serum at 37 °C under 5% CO2 . .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the TransFectin lipid reagent (Bio-Rad Laboratories, Hercules, CA, USA). .. Lipofectamine 3000, or 1.0 mg/mL polyethyleneimine "Max" (PEI-Max) (Polysciences, Inc., Warrington, PA, USA) according to the manufacturer's instructions.

Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
Article Snippet: Cellquest software (Version 5.1, Becton Dickinson, Franklin Lakes, NJ, USA) was used to determine the cell cycle profiles. .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
Article Snippet: Studies of gene transfection in the primary neurons have mainly been based on fetal primary neuron culture and data on gene transfer to the adult cortical neurons are scarce. .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

Mutagenesis:

Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

Plasmid Preparation:

Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro
Article Snippet: .. Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad). .. After 24 hours of transfection, cells were fixed for 20 minutes with 4% paraformaldehyde in phosphate-buffered saline, and then permeabilized with 0.1% Triton X-100 for 10 minutes.

Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential
Article Snippet: The primers of TWIST1 for vector construction is shown in . .. Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and transfection process was performed by Gene Pulser X-cell (Bio-Rad) according to Gene Pulser Electroprotocal. .. Twenty-four to 72 hours after transfection, cells were trypsinized and collected for RNA purification or protein sample preparation.

Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
Article Snippet: .. A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad). .. Transfection was carried out in a 60 mm plate containing eight cover slips with adult neuronal cells and 4 ml of antibiotic free media.

Article Title: Heterologous expression of high-activity cytochrome P450 in mammalian cells
Article Snippet: CYP expression in 293FT cells 293FT cells purchased from ThermoFisher Scientific were cultured in Dulbecco's modified Eagle's medium (Nacalai Tesque) containing 10% foetal bovine serum at 37 °C under 5% CO2 . .. Cells were plated at a density of 2.2 × 106 cells/100-mm dish; 24 h after plating, cells were transfected with a plasmid encoding each CYP , CPR , and CYB cDNA using the TransFectin lipid reagent (Bio-Rad Laboratories, Hercules, CA, USA). .. Lipofectamine 3000, or 1.0 mg/mL polyethyleneimine "Max" (PEI-Max) (Polysciences, Inc., Warrington, PA, USA) according to the manufacturer's instructions.

Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers
Article Snippet: Studies of gene transfection in the primary neurons have mainly been based on fetal primary neuron culture and data on gene transfer to the adult cortical neurons are scarce. .. Transfection of pmaxGFP plasmid to the adult neuron culture described herein, by lipid based transfection reagent resulted in a significant appearance of green fluorescent protein after thirty-six hours. .. Approximately 25-30% transfection efficiency was determined by manual counting of cells.

Construct:

Article Title: The HCV genome domains 5BSL3.1 and 5BSL3.3 act as managers of translation
Article Snippet: .. 5 µg of the constructs IC, ICU, IC_d3.1, IC-d3.2 or IC_d3.3 and 1 µg of the RNA667 were then mixed with 50 µl of Opti-MEM® (Invitrogen) and 2 µl of transfection reagent (TransFectinTM ; Bio-Rad) and added to cell cultures. ..

Expressing:

Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential
Article Snippet: The primers of TWIST1 for vector construction is shown in . .. Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and transfection process was performed by Gene Pulser X-cell (Bio-Rad) according to Gene Pulser Electroprotocal. .. Twenty-four to 72 hours after transfection, cells were trypsinized and collected for RNA purification or protein sample preparation.

Transient Transfection Assay:

Article Title: Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro
Article Snippet: Cellquest software (Version 5.1, Becton Dickinson, Franklin Lakes, NJ, USA) was used to determine the cell cycle profiles. .. Transient Transfection Assay The Transfectin reagent (BioRad, Munich, Germany) was used in the transient transfection assays. .. Ad-MSCs were cultured on plastic or on the fd-ECM.

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    Bio-Rad transfection reagent
    <t>Transfection</t> studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A
    Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection reagent/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transfection reagent - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

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    Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A

    Journal: BMC Medical Genetics

    Article Title: Missense mutations in Desmocollin-2 N-terminus, associated with arrhythmogenic right ventricular cardiomyopathy, affect intracellular localization of desmocollin-2 in vitro

    doi: 10.1186/1471-2350-8-65

    Figure Lengend Snippet: Transfection studies in cultured neonatal rat cardiomyocytes (top panels) and HL-1 cells (bottom panels). Note the WT-DSC2a-GFP was localised at the cell membrane between two cardiomyocytes and HL-1 cells (panel A and D), whereas E102K and I345T-DSC2a-GFP were mainly detected in the cytoplasm (panel B, C, E and F). As a reference, in cardiomyocytes immunostaining of the same cells with a monoclonal anti-alpha-actinin (panel A', B' and C') and overlay of the DSC2a-GFP and alpha-actinin staining (panel A", B" and C") are shown. In HL-1 cells immunostaining with monoclonal desmoglein antibody DG 3.10 showed both the presence of well-assembled desmosomes (panel D', E' and F') and the reduced co-localisation between endogenous dsg and mutated DSC2 (yellow dots in panel E", F").

    Article Snippet: Transfection of neonatal rat cardiomyocytes and HL-1 cells with wild-type and mutant DSC2a-pcDNA3.1/CT After 1 day in culture, cardiomyocytes were transfected with 3 μg of plasmid DNA per well and 8 μl of transfection reagent (transfectin lipid reagent Biorad).

    Techniques: Transfection, Cell Culture, Immunostaining, Staining

    Effect of hypoxia on EOC stem cell differentiation and TWIST-1 expression ( A CD44+/MyD88+ EOC stem cells treated with Cobalt chloride hexahydrate (CCH) showed a significant increase in HIF1a, TWIST-1, and E12 in a time-dependent manner; ( B 5 days in hypoxic and starvation conditions (as described in the Materials and Methods section) promote CD44+/MyD88+ EOC stem cell differentiation as detected by loss of CD44; ( C Stable transfection of shRNA TWIST1 in CD44+/MyD88+ EOC stem cells prevented loss of CD44 when exposed to conditions in B .

    Journal: Oncogene

    Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential

    doi: 10.1038/onc.2012.33

    Figure Lengend Snippet: Effect of hypoxia on EOC stem cell differentiation and TWIST-1 expression ( A CD44+/MyD88+ EOC stem cells treated with Cobalt chloride hexahydrate (CCH) showed a significant increase in HIF1a, TWIST-1, and E12 in a time-dependent manner; ( B 5 days in hypoxic and starvation conditions (as described in the Materials and Methods section) promote CD44+/MyD88+ EOC stem cell differentiation as detected by loss of CD44; ( C Stable transfection of shRNA TWIST1 in CD44+/MyD88+ EOC stem cells prevented loss of CD44 when exposed to conditions in B .

    Article Snippet: Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and transfection process was performed by Gene Pulser X-cell (Bio-Rad) according to Gene Pulser Electroprotocal.

    Techniques: Cell Differentiation, Expressing, Stable Transfection, shRNA

    TWIST-1 expression during EMT ( A Immunofluorescence show specific expression of TWIST-1 only in cells undergoing EMT; (i) CD44+/MyD88+ EOC stem cell monolayer is negative for TWIST-1; (ii) TWIST-1 is expressed in cells with fibroblast/mesenchymal morphology; (iii) TWIST-1 is highly expressed in cells forming spheroids; (iv) Western blot analysis showing TWIST-1 expression is mainly nuclear. EOCSC - CD44+/MyD88+ EOC stem cells; MSFC - mesenchymal-like spheroid forming cells. ( B (i) Transfection of pEMSV- TWIST1 into CD44+/MyD88+ EOC stem cells is not able to induce expression of TWIST-1 protein; (ii-iii) the proteasome inhibitor, MG132 is able to increase endogenous and ectopically expressed TWIST-1. ( C (i) Co-transfection of E12 and TWIST-1 significantly increased TWIST-1 expression in the CD44+/MyD88+ EOC stem cells; ii) parallel increase in E12 and TWIST-1 was observed in the MSFCs and in mouse xenograft. EOCSC - CD44+/MyD88+ EOC stem cells; MSFCs - mesenchymal spheroids forming cells.

    Journal: Oncogene

    Article Title: Constitutive Proteasomal Degradation of TWIST-1 in Epithelial Ovarian Cancer Stem Cells Impacts Differentiation and Metastatic Potential

    doi: 10.1038/onc.2012.33

    Figure Lengend Snippet: TWIST-1 expression during EMT ( A Immunofluorescence show specific expression of TWIST-1 only in cells undergoing EMT; (i) CD44+/MyD88+ EOC stem cell monolayer is negative for TWIST-1; (ii) TWIST-1 is expressed in cells with fibroblast/mesenchymal morphology; (iii) TWIST-1 is highly expressed in cells forming spheroids; (iv) Western blot analysis showing TWIST-1 expression is mainly nuclear. EOCSC - CD44+/MyD88+ EOC stem cells; MSFC - mesenchymal-like spheroid forming cells. ( B (i) Transfection of pEMSV- TWIST1 into CD44+/MyD88+ EOC stem cells is not able to induce expression of TWIST-1 protein; (ii-iii) the proteasome inhibitor, MG132 is able to increase endogenous and ectopically expressed TWIST-1. ( C (i) Co-transfection of E12 and TWIST-1 significantly increased TWIST-1 expression in the CD44+/MyD88+ EOC stem cells; ii) parallel increase in E12 and TWIST-1 was observed in the MSFCs and in mouse xenograft. EOCSC - CD44+/MyD88+ EOC stem cells; MSFCs - mesenchymal spheroids forming cells.

    Article Snippet: Plasmid transfection Ovarian cancer stem cells (5*105 ) were mixed with 10 µg of the indicated expression vectors, and transfection process was performed by Gene Pulser X-cell (Bio-Rad) according to Gene Pulser Electroprotocal.

    Techniques: Expressing, Immunofluorescence, Western Blot, Transfection, Cotransfection

    DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Reporter Gene Assay, Luciferase, Expressing, Construct

    DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Fluorescence, Microscopy, Expressing