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Results of the <t>transcriptome</t> analysis. (A) Heatmap of all genes with higher/lower fold change value than 1/-1 (log2), compared between unstimulated control cells and HEF stimulated cells after 7 and 28 days. (B) Heatmap of top 10 genes with highest and lowest fold changes (log2) each day. (C) Pathways influenced by the electric stimulation. Each node (circle) represents a distinct molecular or biological function, and edges (green lines) represent the number of overlapping genes, determined using a similarity coefficient . Yellow and orange circles represent upregulated molecular or biological functions after 7 (yellow) and 28 days (orange) of HEF stimulation, respectively. Green and blue circles represent downregulated molecular or biological functions after 7 (green) and 28 days (blue) of HEF stimulation, respectively.
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Results of the transcriptome analysis. (A) Heatmap of all genes with higher/lower fold change value than 1/-1 (log2), compared between unstimulated control cells and HEF stimulated cells after 7 and 28 days. (B) Heatmap of top 10 genes with highest and lowest fold changes (log2) each day. (C) Pathways influenced by the electric stimulation. Each node (circle) represents a distinct molecular or biological function, and edges (green lines) represent the number of overlapping genes, determined using a similarity coefficient . Yellow and orange circles represent upregulated molecular or biological functions after 7 (yellow) and 28 days (orange) of HEF stimulation, respectively. Green and blue circles represent downregulated molecular or biological functions after 7 (green) and 28 days (blue) of HEF stimulation, respectively.

Journal: Frontiers in Physiology

Article Title: Long-term stimulation with alternating electric fields modulates the differentiation and mineralization of human pre-osteoblasts

doi: 10.3389/fphys.2022.965181

Figure Lengend Snippet: Results of the transcriptome analysis. (A) Heatmap of all genes with higher/lower fold change value than 1/-1 (log2), compared between unstimulated control cells and HEF stimulated cells after 7 and 28 days. (B) Heatmap of top 10 genes with highest and lowest fold changes (log2) each day. (C) Pathways influenced by the electric stimulation. Each node (circle) represents a distinct molecular or biological function, and edges (green lines) represent the number of overlapping genes, determined using a similarity coefficient . Yellow and orange circles represent upregulated molecular or biological functions after 7 (yellow) and 28 days (orange) of HEF stimulation, respectively. Green and blue circles represent downregulated molecular or biological functions after 7 (green) and 28 days (blue) of HEF stimulation, respectively.

Article Snippet: In the transcriptome analysis data set provided by ATLAS Biolabs, the signal intensity of more than 55,335 annotated probe sets, hence RNA transcripts were determined.

Techniques: Control