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TaKaRa transcription factor overexpression
Western blot to determine effect of formaldehyde and degree of <t>overexpression</t> compared to wild type
Transcription Factor Overexpression, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transcription factor overexpression/product/TaKaRa
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
transcription factor overexpression - by Bioz Stars, 2020-03
79/100 stars

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1) Product Images from "Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method"

Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method

Journal: Methods (San Diego, Calif.)

doi: 10.1016/j.ymeth.2014.10.029

Western blot to determine effect of formaldehyde and degree of overexpression compared to wild type
Figure Legend Snippet: Western blot to determine effect of formaldehyde and degree of overexpression compared to wild type

Techniques Used: Western Blot, Over Expression

Related Articles

Clone Assay:

Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
Article Snippet: .. Yeast strains (see below) Yeast growth medium (YEP, SC supplemented with appropriate carbon source) Plasmid for transcription factor overexpression (see below) In-Fusion HD cloning kit (Clontech) .. 37% formaldehyde (Fisher Scientific) 2.5 M glycine (Bio-Rad) or 3 M Tris base pH 8.0 (Sigma) Benoit Extraction Buffer (200 mM Tris-HCl (pH 8.0), 400 mM (NH4 )2 SO4 , 10 mM MgCl2 , 1 mM EDTA, 10% glycerol, 7 mM β-mercaptoethanol, and protease inhibitors as described below) Protease inhibitors: Roche Complete Protease Inhibitor Cocktail Tablet OR 1.0 mM phenylmethylsulfonyl fluoride, 2.0 mM benzamidine, 2.0 µM pepstatin, 0.6 µM leupeptin, and 2.0 µg of chymostatin per ml of buffer SDS polyacrylamide gel electrophoresis and transfer system (mini-PROTEAN tetra cell, Bio-Rad) Transcription factor-specific primary antibody ECL anti-rabbit or –mouse horseradish peroxidase linked whole antibody (GE Healthcare) Amersham ECL Prime western blotting detecting reagent (GE Healthcare)

Over Expression:

Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
Article Snippet: .. Yeast strains (see below) Yeast growth medium (YEP, SC supplemented with appropriate carbon source) Plasmid for transcription factor overexpression (see below) In-Fusion HD cloning kit (Clontech) .. 37% formaldehyde (Fisher Scientific) 2.5 M glycine (Bio-Rad) or 3 M Tris base pH 8.0 (Sigma) Benoit Extraction Buffer (200 mM Tris-HCl (pH 8.0), 400 mM (NH4 )2 SO4 , 10 mM MgCl2 , 1 mM EDTA, 10% glycerol, 7 mM β-mercaptoethanol, and protease inhibitors as described below) Protease inhibitors: Roche Complete Protease Inhibitor Cocktail Tablet OR 1.0 mM phenylmethylsulfonyl fluoride, 2.0 mM benzamidine, 2.0 µM pepstatin, 0.6 µM leupeptin, and 2.0 µg of chymostatin per ml of buffer SDS polyacrylamide gel electrophoresis and transfer system (mini-PROTEAN tetra cell, Bio-Rad) Transcription factor-specific primary antibody ECL anti-rabbit or –mouse horseradish peroxidase linked whole antibody (GE Healthcare) Amersham ECL Prime western blotting detecting reagent (GE Healthcare)

Plasmid Preparation:

Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method
Article Snippet: .. Yeast strains (see below) Yeast growth medium (YEP, SC supplemented with appropriate carbon source) Plasmid for transcription factor overexpression (see below) In-Fusion HD cloning kit (Clontech) .. 37% formaldehyde (Fisher Scientific) 2.5 M glycine (Bio-Rad) or 3 M Tris base pH 8.0 (Sigma) Benoit Extraction Buffer (200 mM Tris-HCl (pH 8.0), 400 mM (NH4 )2 SO4 , 10 mM MgCl2 , 1 mM EDTA, 10% glycerol, 7 mM β-mercaptoethanol, and protease inhibitors as described below) Protease inhibitors: Roche Complete Protease Inhibitor Cocktail Tablet OR 1.0 mM phenylmethylsulfonyl fluoride, 2.0 mM benzamidine, 2.0 µM pepstatin, 0.6 µM leupeptin, and 2.0 µg of chymostatin per ml of buffer SDS polyacrylamide gel electrophoresis and transfer system (mini-PROTEAN tetra cell, Bio-Rad) Transcription factor-specific primary antibody ECL anti-rabbit or –mouse horseradish peroxidase linked whole antibody (GE Healthcare) Amersham ECL Prime western blotting detecting reagent (GE Healthcare)

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    TaKaRa transcription factor overexpression
    Western blot to determine effect of formaldehyde and degree of <t>overexpression</t> compared to wild type
    Transcription Factor Overexpression, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcription factor overexpression/product/TaKaRa
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transcription factor overexpression - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    Image Search Results


    Western blot to determine effect of formaldehyde and degree of overexpression compared to wild type

    Journal: Methods (San Diego, Calif.)

    Article Title: Analysis of chromatin binding dynamics using the crosslinking kinetics (CLK) method

    doi: 10.1016/j.ymeth.2014.10.029

    Figure Lengend Snippet: Western blot to determine effect of formaldehyde and degree of overexpression compared to wild type

    Article Snippet: Yeast strains (see below) Yeast growth medium (YEP, SC supplemented with appropriate carbon source) Plasmid for transcription factor overexpression (see below) In-Fusion HD cloning kit (Clontech)

    Techniques: Western Blot, Over Expression