Journal: medRxiv

Article Title: External validation of plasma CSF1 as a preoperative prognostic marker in patients with resectable intrahepatic cholangiocarcinoma

doi: 10.1101/2024.11.27.24318028

Figure Lengend Snippet:

Article Snippet: Quantitative Proximity Extension Assay (PEA) was used to determine preoperative CSF1 and TRAIL concentrations and was performed by the Affinity Proteomics Stockholm core facility (SciLifeLab, Stockholm, Sweden), blinded to all clinical and outcome data, using the Olink® Target 48 Cytokine panel (Olink Proteomics AB, Uppsala, Sweden).

Techniques: Comparison, Concentration Assay

Journal: medRxiv

Article Title: External validation of plasma CSF1 as a preoperative prognostic marker in patients with resectable intrahepatic cholangiocarcinoma

doi: 10.1101/2024.11.27.24318028

Figure Lengend Snippet: CSF1, colony stimulating factor 1. TRAIL, TNF-related apoptosis inducing ligand. P-values by log-rank test.

Article Snippet: Quantitative Proximity Extension Assay (PEA) was used to determine preoperative CSF1 and TRAIL concentrations and was performed by the Affinity Proteomics Stockholm core facility (SciLifeLab, Stockholm, Sweden), blinded to all clinical and outcome data, using the Olink® Target 48 Cytokine panel (Olink Proteomics AB, Uppsala, Sweden).

Techniques:

Journal: medRxiv

Article Title: External validation of plasma CSF1 as a preoperative prognostic marker in patients with resectable intrahepatic cholangiocarcinoma

doi: 10.1101/2024.11.27.24318028

Figure Lengend Snippet: CSF1, colony stimulating factor 1. TRAIL, TNF-related apoptosis inducing ligand. P-values by log-rank test.

Article Snippet: Quantitative Proximity Extension Assay (PEA) was used to determine preoperative CSF1 and TRAIL concentrations and was performed by the Affinity Proteomics Stockholm core facility (SciLifeLab, Stockholm, Sweden), blinded to all clinical and outcome data, using the Olink® Target 48 Cytokine panel (Olink Proteomics AB, Uppsala, Sweden).

Techniques:

Journal: PeerJ

Article Title: The role of andrographolide as a potential anticancer agent against gastric cancer cell lines: a systematic review

doi: 10.7717/peerj.18513

Figure Lengend Snippet: Reports of in vitro effects of andrographolide on gastric cancer cells.

Article Snippet: 10–50 µM andrographolide (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) alone or with 5–20 ng/ml recombinant human TRAIL (rhTRAIL; a gift from T.H. Kim, Department of Biochemistry and Molecular Biology, Chosun University, Korea) , Human GC cell lines SNU601, SNU638 and AGS were obtained from the Korean Cell Line Bank (Seoul, Korea) , In vitro cytotoxic activity was assessed by MTT assay. Apoptosis was detected by Hoechst 33,342 (HO)/propidium iodide (PI) double staining. Immunoblotting was done to measure the expression levels of cell cycle inhibitory protein and apoptosis-inducing proteins. Cell proliferating activity was assessed by clonogenic assay. Detection of reactive oxygen species (ROS) generation , Cell growth and cell viability decreased. Maximum apoptotic death occurred when 20 µM of andrographolide was present, while andrographolide triggered non-apoptotic cell death when the concentration exceeded 20 µM. Expression of cyclin inhibitor p21, p27 protein in SNU638 cells, membrane death receptors DR4 (10–20 µm), and DR5 (30–40 µm) were induced. Andrographolide increased p53 levels in AGS and SNU638 cells was increased, but not in SNU601 cells. Clonogenic activity was significantly reduced when a combination of rhTRAIL and andrographolide. ROS increased. , .

Techniques: In Vitro, CCK-8 Assay, Protein Extraction, Protein Concentration, Concentration Assay, Mass Spectrometry, Expressing, Flow Cytometry, Comparison, Activity Assay, MTT Assay, Migration, Wound Healing Assay, Transwell Assay, Western Blot, Zymography, Control, Isolation, Activation Assay, Recombinant, Double Staining, Clonogenic Assay, Membrane, Purification, WST Assay, Inhibition, Cell Cycle Assay, Transmission Assay, Electron Microscopy