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Toyobo total ribonucleic acid rna
Total Ribonucleic Acid Rna, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total ribonucleic acid rna/product/Toyobo
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
total ribonucleic acid rna - by Bioz Stars, 2020-04
94/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer
Article Snippet: .. Real-time PCR TRIzol® Reagent (Life Technologies, Carlsbad, CA) was used to isolate total ribonucleic acid (RNA) using the universal complementary deoxyribonucleic acid (cDNA) synthesis kit (Toyobo, Tokyo, JP). .. The RNA was then reverse-transcribed to obtain cDNA by the universal cDNA synthesis kit (Toyobo, Tokyo, JP) at 37 °C for 50 min. Real-time PCR was performed using the SYBR Green Realtime PCR Master Mix (Toyobo, Tokyo, JP) as previous described [ ].

Article Title: ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer
Article Snippet: Paragraph title: Real-time PCR ... TRIzol® Reagent (Life Technologies, Carlsbad, CA) was used to isolate total ribonucleic acid (RNA) using the universal complementary deoxyribonucleic acid (cDNA) synthesis kit (Toyobo, Tokyo, JP).

SYBR Green Assay:

Article Title: ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer
Article Snippet: Real-time PCR TRIzol® Reagent (Life Technologies, Carlsbad, CA) was used to isolate total ribonucleic acid (RNA) using the universal complementary deoxyribonucleic acid (cDNA) synthesis kit (Toyobo, Tokyo, JP). .. The RNA was then reverse-transcribed to obtain cDNA by the universal cDNA synthesis kit (Toyobo, Tokyo, JP) at 37 °C for 50 min. Real-time PCR was performed using the SYBR Green Realtime PCR Master Mix (Toyobo, Tokyo, JP) as previous described [ ].

Article Title: ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer
Article Snippet: TRIzol® Reagent (Life Technologies, Carlsbad, CA) was used to isolate total ribonucleic acid (RNA) using the universal complementary deoxyribonucleic acid (cDNA) synthesis kit (Toyobo, Tokyo, JP). .. The RNA was then reverse-transcribed to obtain cDNA by the universal cDNA synthesis kit (Toyobo, Tokyo, JP) at 37 °C for 50 min. Real-time PCR was performed using the SYBR Green Realtime PCR Master Mix (Toyobo, Tokyo, JP) as previous described [ ].

Polymerase Chain Reaction:

Article Title: ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer
Article Snippet: Real-time PCR TRIzol® Reagent (Life Technologies, Carlsbad, CA) was used to isolate total ribonucleic acid (RNA) using the universal complementary deoxyribonucleic acid (cDNA) synthesis kit (Toyobo, Tokyo, JP). .. The RNA was then reverse-transcribed to obtain cDNA by the universal cDNA synthesis kit (Toyobo, Tokyo, JP) at 37 °C for 50 min. Real-time PCR was performed using the SYBR Green Realtime PCR Master Mix (Toyobo, Tokyo, JP) as previous described [ ].

Article Title: ACTL6A expression promotes invasion, metastasis and epithelial mesenchymal transition of colon cancer
Article Snippet: TRIzol® Reagent (Life Technologies, Carlsbad, CA) was used to isolate total ribonucleic acid (RNA) using the universal complementary deoxyribonucleic acid (cDNA) synthesis kit (Toyobo, Tokyo, JP). .. The RNA was then reverse-transcribed to obtain cDNA by the universal cDNA synthesis kit (Toyobo, Tokyo, JP) at 37 °C for 50 min. Real-time PCR was performed using the SYBR Green Realtime PCR Master Mix (Toyobo, Tokyo, JP) as previous described [ ].

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  • rna  (Toyobo)
    94
    Toyobo rna
    Expression of Tax mRNA and protein in FPM1V.EFGFP/8R cells was analyzed by <t>RT-PCR</t> and Western blotting. (A) Total RNAs were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 1 μg of each <t>RNA</t> was subjected to RT-PCR analysis for the expression of Tax and G3PDH mRNAs. (B) Whole-cell extracts were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 50 μg of each protein was subjected to Western blotting analysis for the expression of the Tax and α-tubulin proteins.
    Rna, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna/product/Toyobo
    Average 94 stars, based on 255 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    86
    Toyobo quantitative real time pcr qrt pcr isolated total rna
    Changes in the mRNA expression levels of Bmras1 , Bmras2 and Bmras3 during development. mRNA samples were harvested from various organs of various developmental stages from the embryo to adult of B. mori at 24-hours interval. Transcripts of Bmras in these samples were quantified by <t>qRT-PCR.</t> Relative expression levels in whole body (A) and tissues (B) of Bmras1 , whole body (C) and tissues (D) of Bmras2 , whole body (E) and tissues (F) of Bmras3 against Bmrp49 are shown. Expression levels in whole body samples are indicated by solid squares. Changes in organs, namely, the epidermis, fat body, silk gland, muscle, Malpighian tubles and gut are shown by solid circles, solid triangles, open circles, solid diamonds, open squares and open triangles, respectively.
    Quantitative Real Time Pcr Qrt Pcr Isolated Total Rna, supplied by Toyobo, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time pcr qrt pcr isolated total rna/product/Toyobo
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative real time pcr qrt pcr isolated total rna - by Bioz Stars, 2020-04
    86/100 stars
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    Expression of Tax mRNA and protein in FPM1V.EFGFP/8R cells was analyzed by RT-PCR and Western blotting. (A) Total RNAs were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 1 μg of each RNA was subjected to RT-PCR analysis for the expression of Tax and G3PDH mRNAs. (B) Whole-cell extracts were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 50 μg of each protein was subjected to Western blotting analysis for the expression of the Tax and α-tubulin proteins.

    Journal: Journal of Virology

    Article Title: Correlation of Major Histocompatibility Complex Class I Downregulation with Resistance of Human T-Cell Leukemia Virus Type 1-Infected T Cells to Cytotoxic T-Lymphocyte Killing in a Rat Model

    doi: 10.1128/JVI.76.14.7010-7019.2002

    Figure Lengend Snippet: Expression of Tax mRNA and protein in FPM1V.EFGFP/8R cells was analyzed by RT-PCR and Western blotting. (A) Total RNAs were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 1 μg of each RNA was subjected to RT-PCR analysis for the expression of Tax and G3PDH mRNAs. (B) Whole-cell extracts were isolated from FPM1V.EFGFP or FPM1V.EFGFP/8R cells, and 50 μg of each protein was subjected to Western blotting analysis for the expression of the Tax and α-tubulin proteins.

    Article Snippet: One microgram of total RNA was subjected to RT-PCR with an RT-PCR High kit (Toyobo Co.).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Isolation

    Clone and sequence analysis of HongrES2 cDNA. (A) Schematic representation of the 1.3-kb EST screened from a rat epididymal cDNA library, and the new 328 bp sequence obtained from two round 5′ RACE by a BD and Ambion kit, respectively. ( B ) Left panel: Northern blot analysis for HongrES2 with 32 P-labeled 504-bp HongrES2 EST probe1 (seqNo861-1364) . E: epididymis; T: testis; 20 µg total RNA per lane; middle panel: Northern blot analysis for HongrES2 with 32 P-labeled 1978 bp CES7 EST probe2 (seqNo116-2094); right panel: Northern blot analysis for HongrES2 with 32 P-labeled 1191 bp CES7 EST probe3 (seqNo498-1689). ( C ) Upper panel: Chromosomal localization of the HongrES2 gene. The dark gray box is exon 1 from rat chromosome 5, and the light gray box is exon 2 from chromosome 19. The small white box represents the 7-nucleotide overlap from these two exons. e1, exon1; e2, exon2. Lower panel: A sequence alignment of CES7 and HongrES2 . Twelve exons of the CES7 cDNA are depicted in the red rectangle. The same 216- bp fragment of the 3′ end of CES7 exon 12 and HongrES2 exon 2 is depicted by a purple shadow. The location of probe1, probe2 and probe3 for Northern blot analysis in figure1B is also marked out. ( D ) Left panel: RT-PCR validation using primers spanning the chimeric junction portion of HongrES2 cDNA. The product in lane1was got by the primer pairs of upper primer lane1 and common lower primer showed on the right panel. The product in lane2 was got by the primer pairs of upper primer lane2 and common lower primer; right panel: the schematic representation of the location of the two primer pairs used in the RT-PCR. Lane3: negative control; lane4: positive control. (E) Upper panel: ORF analysis of HongrES2 cDNA on line. Three short frames were displayed by rectangles and their sequential number and length were listed. Low panel: The full-length gene sequence of the HongrES2 cDNA. The gray box represents the poly(A) addition signal. The line labels the probe1 sequence used for the northern blot and in situ hybridization in Figure 3A .

    Journal: PLoS ONE

    Article Title: Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation

    doi: 10.1371/journal.pone.0026053

    Figure Lengend Snippet: Clone and sequence analysis of HongrES2 cDNA. (A) Schematic representation of the 1.3-kb EST screened from a rat epididymal cDNA library, and the new 328 bp sequence obtained from two round 5′ RACE by a BD and Ambion kit, respectively. ( B ) Left panel: Northern blot analysis for HongrES2 with 32 P-labeled 504-bp HongrES2 EST probe1 (seqNo861-1364) . E: epididymis; T: testis; 20 µg total RNA per lane; middle panel: Northern blot analysis for HongrES2 with 32 P-labeled 1978 bp CES7 EST probe2 (seqNo116-2094); right panel: Northern blot analysis for HongrES2 with 32 P-labeled 1191 bp CES7 EST probe3 (seqNo498-1689). ( C ) Upper panel: Chromosomal localization of the HongrES2 gene. The dark gray box is exon 1 from rat chromosome 5, and the light gray box is exon 2 from chromosome 19. The small white box represents the 7-nucleotide overlap from these two exons. e1, exon1; e2, exon2. Lower panel: A sequence alignment of CES7 and HongrES2 . Twelve exons of the CES7 cDNA are depicted in the red rectangle. The same 216- bp fragment of the 3′ end of CES7 exon 12 and HongrES2 exon 2 is depicted by a purple shadow. The location of probe1, probe2 and probe3 for Northern blot analysis in figure1B is also marked out. ( D ) Left panel: RT-PCR validation using primers spanning the chimeric junction portion of HongrES2 cDNA. The product in lane1was got by the primer pairs of upper primer lane1 and common lower primer showed on the right panel. The product in lane2 was got by the primer pairs of upper primer lane2 and common lower primer; right panel: the schematic representation of the location of the two primer pairs used in the RT-PCR. Lane3: negative control; lane4: positive control. (E) Upper panel: ORF analysis of HongrES2 cDNA on line. Three short frames were displayed by rectangles and their sequential number and length were listed. Low panel: The full-length gene sequence of the HongrES2 cDNA. The gray box represents the poly(A) addition signal. The line labels the probe1 sequence used for the northern blot and in situ hybridization in Figure 3A .

    Article Snippet: Real-Time PCR For mRNA quantification,a total of 2 ug of RNA prepared as described above was used for reverse transcription, which was performed with a ReverTra Ace-α-TM kit (Toyobo Co., Osaka, Japan) according to the manufacturer's instructions.

    Techniques: Sequencing, cDNA Library Assay, Northern Blot, Labeling, Reverse Transcription Polymerase Chain Reaction, Negative Control, Positive Control, In Situ Hybridization

    HongrES2 RNA is the precursor of a new miRNA-like small RNA. (A) Panel 1: the in situ hybridization shows the subcellular localization of the 1.6-kb HongrES2 transcript in the cauda region. Panel 2: the in situ hybridization shows the cytoplasmic localization of the Bin1b transcript in the caput region as a control (100×); bar = 125 µm. Panel 3: the in situ hybridization shows the nuclear location of U6 RNA in the corpus region as a positive control (40×); bar = 200 µm. ( B ) The secondary structure prediction of the HongrES2 RNA by mfold. The predicted stem loop structure and mature sequence are labeled with purple and yellow, respectively. ( C ) A schematic representation of the different cDNA fragments described in (B). ( D ) Northern blot analysis showing three forms of the HongrES2 RNA in the rat epididymis. CD: cauda; CO: corpus; CP: caput; marker: Ambion small RNA marker (10 nt–100 nt). The marker lane was exposed for a shorter time on the same membrane. The blastn search result of the mil-HongrES2 sequence against the miRBase database. ( E ) Upper panel: depiction of how the mil-HongrES2 was bailed from the small RNA library by selective primers (GSP 3′adaptor primer). Low pannel: the mil-HongrES2 sequences identified from the small RNA library and the miRNA:miRNA* like duplex sequence. Real-time PCR showed the camparative quantitation of the mil-HongrES2 molecules in the whole small RNA library. ( F ) HongrES2 is processed into mil-HongrES2 in PC1 cells. Lane1: RNA from PC1 cells transfected with the HongrES2 expression vector. Lane2: The 25 nt RNA oligo nucleotide used as a positive control and the size marker for the processed product. The strong band below pre form of mil-HongrES2 pointed out by arrow was analysed through poly(A) tailing PCR using the gene specific forward primer. ( G ) Northern blotting to detect mil-HongrES2 after anti-FLAG-Ago2 immunoprecipitation. The right panel shows PC1 cells that were transfected with HongrES2 expression plasmids alone (mock) or in combination with plasmids that express FLAG-tagged Argonaute2 (Flag-Ago2). The left panel shows a control experiment using a probe of a known mir-29a to demonstrate that the IP worked. ( H ) The left panel shows that the Dicer protein level was reduced by two different siRNAs. The right panel shows that mil-HongrES2 expression was inhibited in DCR knockdown cells. Mir-29a expression was also detected as a control to show that the whole RNAi experiment was effective. Nosi: control siRNA. siDCR1/2: two different sequences of the siRNA-targeting mouse DCR gene. ( I ) Upper panel :sequence alignment of the 100-bp conserved sequence between different species of rat, mouse, and human around the mil-HongrES2 encoding region. Red rectangles labeled out the mil-HongrES2 sequence. Lower panel: northern blot analysis of mouse mil-HongrES2 expression, using the rat LNA probe of mil-HongrES2 (24 nt). Hybridization was carried out at 42°C.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation

    doi: 10.1371/journal.pone.0026053

    Figure Lengend Snippet: HongrES2 RNA is the precursor of a new miRNA-like small RNA. (A) Panel 1: the in situ hybridization shows the subcellular localization of the 1.6-kb HongrES2 transcript in the cauda region. Panel 2: the in situ hybridization shows the cytoplasmic localization of the Bin1b transcript in the caput region as a control (100×); bar = 125 µm. Panel 3: the in situ hybridization shows the nuclear location of U6 RNA in the corpus region as a positive control (40×); bar = 200 µm. ( B ) The secondary structure prediction of the HongrES2 RNA by mfold. The predicted stem loop structure and mature sequence are labeled with purple and yellow, respectively. ( C ) A schematic representation of the different cDNA fragments described in (B). ( D ) Northern blot analysis showing three forms of the HongrES2 RNA in the rat epididymis. CD: cauda; CO: corpus; CP: caput; marker: Ambion small RNA marker (10 nt–100 nt). The marker lane was exposed for a shorter time on the same membrane. The blastn search result of the mil-HongrES2 sequence against the miRBase database. ( E ) Upper panel: depiction of how the mil-HongrES2 was bailed from the small RNA library by selective primers (GSP 3′adaptor primer). Low pannel: the mil-HongrES2 sequences identified from the small RNA library and the miRNA:miRNA* like duplex sequence. Real-time PCR showed the camparative quantitation of the mil-HongrES2 molecules in the whole small RNA library. ( F ) HongrES2 is processed into mil-HongrES2 in PC1 cells. Lane1: RNA from PC1 cells transfected with the HongrES2 expression vector. Lane2: The 25 nt RNA oligo nucleotide used as a positive control and the size marker for the processed product. The strong band below pre form of mil-HongrES2 pointed out by arrow was analysed through poly(A) tailing PCR using the gene specific forward primer. ( G ) Northern blotting to detect mil-HongrES2 after anti-FLAG-Ago2 immunoprecipitation. The right panel shows PC1 cells that were transfected with HongrES2 expression plasmids alone (mock) or in combination with plasmids that express FLAG-tagged Argonaute2 (Flag-Ago2). The left panel shows a control experiment using a probe of a known mir-29a to demonstrate that the IP worked. ( H ) The left panel shows that the Dicer protein level was reduced by two different siRNAs. The right panel shows that mil-HongrES2 expression was inhibited in DCR knockdown cells. Mir-29a expression was also detected as a control to show that the whole RNAi experiment was effective. Nosi: control siRNA. siDCR1/2: two different sequences of the siRNA-targeting mouse DCR gene. ( I ) Upper panel :sequence alignment of the 100-bp conserved sequence between different species of rat, mouse, and human around the mil-HongrES2 encoding region. Red rectangles labeled out the mil-HongrES2 sequence. Lower panel: northern blot analysis of mouse mil-HongrES2 expression, using the rat LNA probe of mil-HongrES2 (24 nt). Hybridization was carried out at 42°C.

    Article Snippet: Real-Time PCR For mRNA quantification,a total of 2 ug of RNA prepared as described above was used for reverse transcription, which was performed with a ReverTra Ace-α-TM kit (Toyobo Co., Osaka, Japan) according to the manufacturer's instructions.

    Techniques: In Situ Hybridization, Positive Control, Sequencing, Labeling, Northern Blot, Marker, Real-time Polymerase Chain Reaction, Quantitation Assay, Transfection, Expressing, Plasmid Preparation, Polymerase Chain Reaction, Immunoprecipitation, Hybridization

    Over-expression of mil-HongrES2 caused by inflammation of the rat epididymitis. ( A ) The upper panel showed Northern blot analysis of the RNA expression of HongrES2 .18S rRNA was used as the loading control. The lower panel was the Real-time PCR analysis of Ncp2 besides HongrES2 . ( B,C ) Northern blot analysis to detect the mil-HongrES2 expression. U6 probe was used on the stripped membrane as an internal loading control.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation

    doi: 10.1371/journal.pone.0026053

    Figure Lengend Snippet: Over-expression of mil-HongrES2 caused by inflammation of the rat epididymitis. ( A ) The upper panel showed Northern blot analysis of the RNA expression of HongrES2 .18S rRNA was used as the loading control. The lower panel was the Real-time PCR analysis of Ncp2 besides HongrES2 . ( B,C ) Northern blot analysis to detect the mil-HongrES2 expression. U6 probe was used on the stripped membrane as an internal loading control.

    Article Snippet: Real-Time PCR For mRNA quantification,a total of 2 ug of RNA prepared as described above was used for reverse transcription, which was performed with a ReverTra Ace-α-TM kit (Toyobo Co., Osaka, Japan) according to the manufacturer's instructions.

    Techniques: Over Expression, Northern Blot, RNA Expression, Real-time Polymerase Chain Reaction, Expressing

    Regional and temporal expression of HongrES2 RNA. ( A ) The tissue distribution of HongrES2 RNA. ( B, C ) The region-specific expression pattern, as shown by northern blot analysis, QRT-PCR ,and in situ hybridization. CP:caput; CO:corpus; CD: cauda; an 18S probe was used on the stripped membrane as an internal loading control. Bar = 2.0 mm. The primers used for RT-PCR ,Real-time PCR was listed in Table1. ( D ) The upper panel shows the northern blot analysis of HongrES2 RNA and 18S rRNA during development. The lower panel shows the relative amounts of HongrES2 RNA in the rat epididymis at different developmental stages. ( E ) The upper panel shows Northern blot analysis of adult rat epididymal HongrES2 RNA. level from pre-castration (d0) and castration for 1, 3, 5, and 7 days (d1, d3, d5, and d7) as well as for 1, 3, 5, and 7 days after the initial injection of testosterone propionate applied to the 7d-castrated rats (d7+1, d7+3, d7 +5, and d7 +7). Injections were continued every 2 days. The lower panel showed the relative expression levels of HongrES2 RNA (hybridization density of HongrES2 RNA/18S ribosomal RNA) in the rat.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation

    doi: 10.1371/journal.pone.0026053

    Figure Lengend Snippet: Regional and temporal expression of HongrES2 RNA. ( A ) The tissue distribution of HongrES2 RNA. ( B, C ) The region-specific expression pattern, as shown by northern blot analysis, QRT-PCR ,and in situ hybridization. CP:caput; CO:corpus; CD: cauda; an 18S probe was used on the stripped membrane as an internal loading control. Bar = 2.0 mm. The primers used for RT-PCR ,Real-time PCR was listed in Table1. ( D ) The upper panel shows the northern blot analysis of HongrES2 RNA and 18S rRNA during development. The lower panel shows the relative amounts of HongrES2 RNA in the rat epididymis at different developmental stages. ( E ) The upper panel shows Northern blot analysis of adult rat epididymal HongrES2 RNA. level from pre-castration (d0) and castration for 1, 3, 5, and 7 days (d1, d3, d5, and d7) as well as for 1, 3, 5, and 7 days after the initial injection of testosterone propionate applied to the 7d-castrated rats (d7+1, d7+3, d7 +5, and d7 +7). Injections were continued every 2 days. The lower panel showed the relative expression levels of HongrES2 RNA (hybridization density of HongrES2 RNA/18S ribosomal RNA) in the rat.

    Article Snippet: Real-Time PCR For mRNA quantification,a total of 2 ug of RNA prepared as described above was used for reverse transcription, which was performed with a ReverTra Ace-α-TM kit (Toyobo Co., Osaka, Japan) according to the manufacturer's instructions.

    Techniques: Expressing, Northern Blot, Quantitative RT-PCR, In Situ Hybridization, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Injection, Hybridization

    mRNA levels of ICP4 (a) and ICP0 (b) in EHV-1 attB-, EHV-1∆VP22- and EHV-1∆VP22R-infected cells. MDBK cells were inoculated with EHV-1 attB, EHV-1∆VP22 and EHV-1∆VP22R at an MOI of 3. After 0 and 1 hrpi, total RNA and DNA was extracted. Quantification of mRNA was carried out by real-time quantitative RT-PCR. Expression levels of ICP4 and ICP0 mRNA were normalized with the GAPDH mRNA levels and genome DNA, and expressed relatively as the ratio against the mRNA levels in EHV-1 attB-infected cells at 0 hrpi. There are no significant (n.s.) differences between EHV-1 attB and EHV-1∆VP22. * means “below the threshold”.

    Journal: The Journal of Veterinary Medical Science

    Article Title: Decreased expression of the immediate early protein, ICP4, by deletion of the tegument protein VP22 of equine herpesvirus type 1

    doi: 10.1292/jvms.17-0380

    Figure Lengend Snippet: mRNA levels of ICP4 (a) and ICP0 (b) in EHV-1 attB-, EHV-1∆VP22- and EHV-1∆VP22R-infected cells. MDBK cells were inoculated with EHV-1 attB, EHV-1∆VP22 and EHV-1∆VP22R at an MOI of 3. After 0 and 1 hrpi, total RNA and DNA was extracted. Quantification of mRNA was carried out by real-time quantitative RT-PCR. Expression levels of ICP4 and ICP0 mRNA were normalized with the GAPDH mRNA levels and genome DNA, and expressed relatively as the ratio against the mRNA levels in EHV-1 attB-infected cells at 0 hrpi. There are no significant (n.s.) differences between EHV-1 attB and EHV-1∆VP22. * means “below the threshold”.

    Article Snippet: Total RNA and DNA of infected cells were extracted using AllPrep DNA/RNA Mini Kit (QIAGEN K.K., Hilden, Germany) at 0 and 1 hrpi. cDNA was synthesized from 0.5 µ g of total RNA using ReverTra Ace (TOYOBO, Osaka, Japan).

    Techniques: Infection, Quantitative RT-PCR, Expressing

    Changes in the mRNA expression levels of Bmras1 , Bmras2 and Bmras3 during development. mRNA samples were harvested from various organs of various developmental stages from the embryo to adult of B. mori at 24-hours interval. Transcripts of Bmras in these samples were quantified by qRT-PCR. Relative expression levels in whole body (A) and tissues (B) of Bmras1 , whole body (C) and tissues (D) of Bmras2 , whole body (E) and tissues (F) of Bmras3 against Bmrp49 are shown. Expression levels in whole body samples are indicated by solid squares. Changes in organs, namely, the epidermis, fat body, silk gland, muscle, Malpighian tubles and gut are shown by solid circles, solid triangles, open circles, solid diamonds, open squares and open triangles, respectively.

    Journal: PLoS ONE

    Article Title: Identification and Expression Analysis of Ras Gene in Silkworm, Bombyx mori

    doi: 10.1371/journal.pone.0008030

    Figure Lengend Snippet: Changes in the mRNA expression levels of Bmras1 , Bmras2 and Bmras3 during development. mRNA samples were harvested from various organs of various developmental stages from the embryo to adult of B. mori at 24-hours interval. Transcripts of Bmras in these samples were quantified by qRT-PCR. Relative expression levels in whole body (A) and tissues (B) of Bmras1 , whole body (C) and tissues (D) of Bmras2 , whole body (E) and tissues (F) of Bmras3 against Bmrp49 are shown. Expression levels in whole body samples are indicated by solid squares. Changes in organs, namely, the epidermis, fat body, silk gland, muscle, Malpighian tubles and gut are shown by solid circles, solid triangles, open circles, solid diamonds, open squares and open triangles, respectively.

    Article Snippet: Quantitative Real-Time PCR (qRT-PCR) Isolated total RNA was reverse transcribed using ReverTra -Plus - (TOYOBO) following the manufacturer's instruction.

    Techniques: Expressing, Quantitative RT-PCR