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Bioteke Corporation total ribonucleic acid rna
Total Ribonucleic Acid Rna, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway
Article Snippet: Paragraph title: Quantitative real-time polymerase chain reaction (qRT-PCR) ... Total ribonucleic acid (RNA) was extracted with a High-purity Total RNA Fast Extraction Kit (BioTeke, Beijing, China) and reversely transcribed to complementary deoxyribonucleic acid (cDNA).

Quantitative RT-PCR:

Article Title: Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway
Article Snippet: Paragraph title: Quantitative real-time polymerase chain reaction (qRT-PCR) ... Total ribonucleic acid (RNA) was extracted with a High-purity Total RNA Fast Extraction Kit (BioTeke, Beijing, China) and reversely transcribed to complementary deoxyribonucleic acid (cDNA).

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    Bioteke Corporation real time pcr analysis total rna
    Relative expression levels analysis of transcripts positively associated with dormancy release ( CYP707A , GA20ox2 , GA20ox3 , IAAH , IAAS , BRRK ,) in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification processes. RS, radical just sprouted; RG, radical growing up to 1.5 cm. Expression is shown relative to GAPDH as the reference gene. <t>RNA</t> was extracted from three biological samples and <t>qRT-PCR</t> was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P
    Real Time Pcr Analysis Total Rna, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr analysis total rna/product/Bioteke Corporation
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    real time pcr analysis total rna - by Bioz Stars, 2020-04
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    88
    Bioteke Corporation quantitative real time reverse transcription pcr qrt pcr total rna
    Expression of SmDCLs in flowers (Fl), leaves (Le), stems (St) and roots (Rt) of S. miltiorrhiza. Expression levels were quantified by <t>qRT-PCR.</t> The levels in roots were arbitrarily set to 1. Error bars represent the standard deviations of three technical PCR replicates.
    Quantitative Real Time Reverse Transcription Pcr Qrt Pcr Total Rna, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time reverse transcription pcr qrt pcr total rna/product/Bioteke Corporation
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    quantitative real time reverse transcription pcr qrt pcr total rna - by Bioz Stars, 2020-04
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    92
    Bioteke Corporation quantitative reverse transcription polymerase chain reaction qrt pcr total rna
    Ang II induces a rapid upregulation of CTGF expression independently of TGF- β in LX-2 cells. ( A ) Serum-starved LX-2 cells were stimulated with Ang II (10 −8 –10 −6 M) for 4 h. Whole cell lysates were immunoblotted with CTGF antibody and a representative immunoblot band of CTGF from 3 independent experiments is shown. β -Actin was used as an internal control for equal protein loading. ( B ) Results of total CTGF expression were obtained from densitometric analysis and expressed as ratio CTGF/ β -actin, as n -fold increase over that in unstimulated control cells. ( C,F ) Serum-starved LX-2 cells were exposed to Ang II (10 −7 M) for 0, 0.25, 0.5, 1, 2 and 4 h. The mRNA expression of the TGF-β1 and CTGF gene was measured by the <t>qRT-PCR</t> method as described in the Materials and Methods section. ( D,G ) Serum-starved LX-2 cells were exposed to Ang II (10 −7 M) for 0, 0.5, 1, 2, 4, 8, 16, 24 and 48 h. Whole cell lysates were prepared and immunoblotted with anti-CTGF or anti-TGF- β 1 antibodies, respectively. Anti- β -actin antibody was used to demonstrate equal protein loading. Similar results were observed in 3 independent experiments, and representative immunoblot bands of CTGF and TGF- β 1 are shown. ( E,H ) Quantitative determination of CTGF and TGF- β 1 protein levels at various time points as indicated, which were converted to arbitrary densitometric units, normalized by the value of β -actin and finally expressed as n -fold change over that of unstimulated control cells (defined as 1.0). ( I,J ) TGF- β signaling was blocked by pretreatment for 1 h with SB-431542 (a TGF- β receptor kinase inhibitor; 10 −5 M), ( K,L ) or by transfection with TGF- β 1 siRNA, before incubation with or without Ang II (10 −7 M) for 1, 4 and 24 h. The CTGF and TGF- β 1 protein levels were analyzed by Western blotting. As a positive control of CTGF production, TGF- β 1 (10 ng/mL) was used. Data are presented as mean ± SD of 3 independent experiments. # P
    Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr Total Rna, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative reverse transcription polymerase chain reaction qrt pcr total rna/product/Bioteke Corporation
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative reverse transcription polymerase chain reaction qrt pcr total rna - by Bioz Stars, 2020-04
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    93
    Bioteke Corporation q rt pcr analysis total rna
    Metabolism and regulation of ABA and SL biosynthesis in N. incisum seeds. Up-regulated genes are shown in red, down-regulated genes are shown in blue, genes with no significant change in expression are shown in black, and genes not identified are shown in gray. The inset graphs show relative expression levels of the major ABA genes in FL compared to Con detected by <t>RT-PCR</t> ( a ) or <t>RNA-Seq</t> ( b ), and the major SLs genes in FL and CS compared to Con detected by RT-PCR ( c ). Data represent means ± SD ( n = 3)
    Q Rt Pcr Analysis Total Rna, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q rt pcr analysis total rna/product/Bioteke Corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q rt pcr analysis total rna - by Bioz Stars, 2020-04
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    Relative expression levels analysis of transcripts positively associated with dormancy release ( CYP707A , GA20ox2 , GA20ox3 , IAAH , IAAS , BRRK ,) in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification processes. RS, radical just sprouted; RG, radical growing up to 1.5 cm. Expression is shown relative to GAPDH as the reference gene. RNA was extracted from three biological samples and qRT-PCR was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P

    Journal: BMC Genomics

    Article Title: Mining genes involved in the stratification of Paris Polyphylla seeds using high-throughput embryo Transcriptome sequencing

    doi: 10.1186/1471-2164-14-358

    Figure Lengend Snippet: Relative expression levels analysis of transcripts positively associated with dormancy release ( CYP707A , GA20ox2 , GA20ox3 , IAAH , IAAS , BRRK ,) in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification processes. RS, radical just sprouted; RG, radical growing up to 1.5 cm. Expression is shown relative to GAPDH as the reference gene. RNA was extracted from three biological samples and qRT-PCR was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P

    Article Snippet: Real-time PCR analysis Total RNA was extracted from embryos and endosperms from warm and cold seed stratification stages using the RNeasy Plant kit (BioTeke, Beijing China).

    Techniques: Expressing, Quantitative RT-PCR

    Relative expression levels analysis of transcripts negatively associated with dormancy release (NCED, ABI2, PP2C, ARP3 , ARP7 , DRM ) levels in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification process. RS, radical just sprouted; RG, radical growing up to 2 cm. Expression is shown relative to GAPDH as the reference gene. RNA was extracted from three biological samples and qRT-PCR was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P

    Journal: BMC Genomics

    Article Title: Mining genes involved in the stratification of Paris Polyphylla seeds using high-throughput embryo Transcriptome sequencing

    doi: 10.1186/1471-2164-14-358

    Figure Lengend Snippet: Relative expression levels analysis of transcripts negatively associated with dormancy release (NCED, ABI2, PP2C, ARP3 , ARP7 , DRM ) levels in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification process. RS, radical just sprouted; RG, radical growing up to 2 cm. Expression is shown relative to GAPDH as the reference gene. RNA was extracted from three biological samples and qRT-PCR was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P

    Article Snippet: Real-time PCR analysis Total RNA was extracted from embryos and endosperms from warm and cold seed stratification stages using the RNeasy Plant kit (BioTeke, Beijing China).

    Techniques: Expressing, Quantitative RT-PCR

    Relative expression levels analysis of four circadian rhythms related transcipts ( ELF1 , ELF2 , SFR6 , SUS ) in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification process. RS, radical just sprouted; RG, radical growing up to 2 cm. Expression is shown relative to GAPDH as the reference gene. RNA was extracted from three biological samples and qRT-PCR was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P

    Journal: BMC Genomics

    Article Title: Mining genes involved in the stratification of Paris Polyphylla seeds using high-throughput embryo Transcriptome sequencing

    doi: 10.1186/1471-2164-14-358

    Figure Lengend Snippet: Relative expression levels analysis of four circadian rhythms related transcipts ( ELF1 , ELF2 , SFR6 , SUS ) in the Embryo (Em□) and endosperm (En■) under warm (20°C)/cold (4°C) seed stratification process. RS, radical just sprouted; RG, radical growing up to 2 cm. Expression is shown relative to GAPDH as the reference gene. RNA was extracted from three biological samples and qRT-PCR was performed. Data represent means ± SD. * indicate a significant difference between Em and En at P

    Article Snippet: Real-time PCR analysis Total RNA was extracted from embryos and endosperms from warm and cold seed stratification stages using the RNeasy Plant kit (BioTeke, Beijing China).

    Techniques: Expressing, Quantitative RT-PCR

    Expression of SmDCLs in flowers (Fl), leaves (Le), stems (St) and roots (Rt) of S. miltiorrhiza. Expression levels were quantified by qRT-PCR. The levels in roots were arbitrarily set to 1. Error bars represent the standard deviations of three technical PCR replicates.

    Journal: Scientific Reports

    Article Title: Comparative analysis of the Dicer-like gene family reveals loss of miR162 target site in SmDCL1 from Salvia miltiorrhiza

    doi: 10.1038/srep09891

    Figure Lengend Snippet: Expression of SmDCLs in flowers (Fl), leaves (Le), stems (St) and roots (Rt) of S. miltiorrhiza. Expression levels were quantified by qRT-PCR. The levels in roots were arbitrarily set to 1. Error bars represent the standard deviations of three technical PCR replicates.

    Article Snippet: Quantitative real-time reverse transcription-PCR (qRT-PCR) Total RNA was isolated from plant tissues using the plant total RNA extraction kit (BioTeke, Beijing, China) and genomic DNA was removed by treating with RNase-free DNase (Promega, Madison, WI, USA).

    Techniques: Expressing, Quantitative RT-PCR, Polymerase Chain Reaction

    The Sm-MIR397 precursor and complementarities between miRNAs and SmDCL1. ( a ) Predicted hairpin structures of Sm-MIR397 . Mature miRNA sequences are indicated in red. Vertical lines indicate G:C and A:U pairings. Circles indicate G:U pairings. ( b ) Complementarities between Sm-miR397, At-miR162a/b and SmDCL1. The heavy black line represents ORF. The lines flanking ORF represent nontranslated regions. MiRNA complementary sites with the nucleotide positions of SmDCL1 cDNA are indicated. The RNA sequence of each complementary site from 5′ to 3′ and the predicted miRNA sequence from 3′ to 5′ are shown in the expanded regions. Arrows indicate the 5′ termini of three cDNA fragments ( c ) with the frequency of clones (in parentheses) and the nucleotide positions of SmDCL1 cDNA shown. ( c ) Determination of the 5′ termini of truncated SmDCL1 cDNA fragments using the 5′-RACE method. Nested PCR products were separated in a 2% agarose gel.

    Journal: Scientific Reports

    Article Title: Comparative analysis of the Dicer-like gene family reveals loss of miR162 target site in SmDCL1 from Salvia miltiorrhiza

    doi: 10.1038/srep09891

    Figure Lengend Snippet: The Sm-MIR397 precursor and complementarities between miRNAs and SmDCL1. ( a ) Predicted hairpin structures of Sm-MIR397 . Mature miRNA sequences are indicated in red. Vertical lines indicate G:C and A:U pairings. Circles indicate G:U pairings. ( b ) Complementarities between Sm-miR397, At-miR162a/b and SmDCL1. The heavy black line represents ORF. The lines flanking ORF represent nontranslated regions. MiRNA complementary sites with the nucleotide positions of SmDCL1 cDNA are indicated. The RNA sequence of each complementary site from 5′ to 3′ and the predicted miRNA sequence from 3′ to 5′ are shown in the expanded regions. Arrows indicate the 5′ termini of three cDNA fragments ( c ) with the frequency of clones (in parentheses) and the nucleotide positions of SmDCL1 cDNA shown. ( c ) Determination of the 5′ termini of truncated SmDCL1 cDNA fragments using the 5′-RACE method. Nested PCR products were separated in a 2% agarose gel.

    Article Snippet: Quantitative real-time reverse transcription-PCR (qRT-PCR) Total RNA was isolated from plant tissues using the plant total RNA extraction kit (BioTeke, Beijing, China) and genomic DNA was removed by treating with RNase-free DNase (Promega, Madison, WI, USA).

    Techniques: Sequencing, Clone Assay, Nested PCR, Agarose Gel Electrophoresis

    Ang II induces a rapid upregulation of CTGF expression independently of TGF- β in LX-2 cells. ( A ) Serum-starved LX-2 cells were stimulated with Ang II (10 −8 –10 −6 M) for 4 h. Whole cell lysates were immunoblotted with CTGF antibody and a representative immunoblot band of CTGF from 3 independent experiments is shown. β -Actin was used as an internal control for equal protein loading. ( B ) Results of total CTGF expression were obtained from densitometric analysis and expressed as ratio CTGF/ β -actin, as n -fold increase over that in unstimulated control cells. ( C,F ) Serum-starved LX-2 cells were exposed to Ang II (10 −7 M) for 0, 0.25, 0.5, 1, 2 and 4 h. The mRNA expression of the TGF-β1 and CTGF gene was measured by the qRT-PCR method as described in the Materials and Methods section. ( D,G ) Serum-starved LX-2 cells were exposed to Ang II (10 −7 M) for 0, 0.5, 1, 2, 4, 8, 16, 24 and 48 h. Whole cell lysates were prepared and immunoblotted with anti-CTGF or anti-TGF- β 1 antibodies, respectively. Anti- β -actin antibody was used to demonstrate equal protein loading. Similar results were observed in 3 independent experiments, and representative immunoblot bands of CTGF and TGF- β 1 are shown. ( E,H ) Quantitative determination of CTGF and TGF- β 1 protein levels at various time points as indicated, which were converted to arbitrary densitometric units, normalized by the value of β -actin and finally expressed as n -fold change over that of unstimulated control cells (defined as 1.0). ( I,J ) TGF- β signaling was blocked by pretreatment for 1 h with SB-431542 (a TGF- β receptor kinase inhibitor; 10 −5 M), ( K,L ) or by transfection with TGF- β 1 siRNA, before incubation with or without Ang II (10 −7 M) for 1, 4 and 24 h. The CTGF and TGF- β 1 protein levels were analyzed by Western blotting. As a positive control of CTGF production, TGF- β 1 (10 ng/mL) was used. Data are presented as mean ± SD of 3 independent experiments. # P

    Journal: Scientific Reports

    Article Title: Angiotensin II induces connective tissue growth factor expression in human hepatic stellate cells by a transforming growth factor β-independent mechanism

    doi: 10.1038/s41598-017-08334-x

    Figure Lengend Snippet: Ang II induces a rapid upregulation of CTGF expression independently of TGF- β in LX-2 cells. ( A ) Serum-starved LX-2 cells were stimulated with Ang II (10 −8 –10 −6 M) for 4 h. Whole cell lysates were immunoblotted with CTGF antibody and a representative immunoblot band of CTGF from 3 independent experiments is shown. β -Actin was used as an internal control for equal protein loading. ( B ) Results of total CTGF expression were obtained from densitometric analysis and expressed as ratio CTGF/ β -actin, as n -fold increase over that in unstimulated control cells. ( C,F ) Serum-starved LX-2 cells were exposed to Ang II (10 −7 M) for 0, 0.25, 0.5, 1, 2 and 4 h. The mRNA expression of the TGF-β1 and CTGF gene was measured by the qRT-PCR method as described in the Materials and Methods section. ( D,G ) Serum-starved LX-2 cells were exposed to Ang II (10 −7 M) for 0, 0.5, 1, 2, 4, 8, 16, 24 and 48 h. Whole cell lysates were prepared and immunoblotted with anti-CTGF or anti-TGF- β 1 antibodies, respectively. Anti- β -actin antibody was used to demonstrate equal protein loading. Similar results were observed in 3 independent experiments, and representative immunoblot bands of CTGF and TGF- β 1 are shown. ( E,H ) Quantitative determination of CTGF and TGF- β 1 protein levels at various time points as indicated, which were converted to arbitrary densitometric units, normalized by the value of β -actin and finally expressed as n -fold change over that of unstimulated control cells (defined as 1.0). ( I,J ) TGF- β signaling was blocked by pretreatment for 1 h with SB-431542 (a TGF- β receptor kinase inhibitor; 10 −5 M), ( K,L ) or by transfection with TGF- β 1 siRNA, before incubation with or without Ang II (10 −7 M) for 1, 4 and 24 h. The CTGF and TGF- β 1 protein levels were analyzed by Western blotting. As a positive control of CTGF production, TGF- β 1 (10 ng/mL) was used. Data are presented as mean ± SD of 3 independent experiments. # P

    Article Snippet: Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) Total RNA was isolated from unstimulated control or Ang II-stimulated cell pellets using a RNApure high-purity total RNA rapid extraction kit (spin-column, BioTeke, Beijing, China) according to the manufacturer’s protocols.

    Techniques: Expressing, Quantitative RT-PCR, Transfection, Incubation, Western Blot, Positive Control

    Metabolism and regulation of ABA and SL biosynthesis in N. incisum seeds. Up-regulated genes are shown in red, down-regulated genes are shown in blue, genes with no significant change in expression are shown in black, and genes not identified are shown in gray. The inset graphs show relative expression levels of the major ABA genes in FL compared to Con detected by RT-PCR ( a ) or RNA-Seq ( b ), and the major SLs genes in FL and CS compared to Con detected by RT-PCR ( c ). Data represent means ± SD ( n = 3)

    Journal: BMC Plant Biology

    Article Title: Molecular mechanism of seed dormancy release induced by fluridone compared with cod stratification in Notopterygium incisum

    doi: 10.1186/s12870-018-1333-2

    Figure Lengend Snippet: Metabolism and regulation of ABA and SL biosynthesis in N. incisum seeds. Up-regulated genes are shown in red, down-regulated genes are shown in blue, genes with no significant change in expression are shown in black, and genes not identified are shown in gray. The inset graphs show relative expression levels of the major ABA genes in FL compared to Con detected by RT-PCR ( a ) or RNA-Seq ( b ), and the major SLs genes in FL and CS compared to Con detected by RT-PCR ( c ). Data represent means ± SD ( n = 3)

    Article Snippet: Q-RT-PCR analysis Total RNA was isolated from embryos using a Universal Plant RNeasy kit (BioTeke, China).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, RNA Sequencing Assay

    Comparison of relative gene expression levels detected by RT-PCR vs. RNA-Seq (read count). The data are log 2 -transformed values of the fold changes in FL compared to Con for N. incisum seeds

    Journal: BMC Plant Biology

    Article Title: Molecular mechanism of seed dormancy release induced by fluridone compared with cod stratification in Notopterygium incisum

    doi: 10.1186/s12870-018-1333-2

    Figure Lengend Snippet: Comparison of relative gene expression levels detected by RT-PCR vs. RNA-Seq (read count). The data are log 2 -transformed values of the fold changes in FL compared to Con for N. incisum seeds

    Article Snippet: Q-RT-PCR analysis Total RNA was isolated from embryos using a Universal Plant RNeasy kit (BioTeke, China).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, RNA Sequencing Assay, Transformation Assay