Structured Review

Santa Cruz Biotechnology total p38 mapk sc 7149
A) Seminiferous tubule cells from rats injected with 50 mg/kg of BPA or NP were isolated as described in the section and incubated with and antibody against the active form of ADAM17. The graph shows that the percentage of cells labeled with the antibody increases in rats treated with BPA or NP, but is reduced when rats were treated in the presence of GW280264X, a pharmacological inhibitor of ADAM17 (GW), or PD169316, a pharmacological inhibitor of <t>p38</t> <t>MAPK</t> (PD). * p<0.05, n = 3.
Total P38 Mapk Sc 7149, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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1) Product Images from "A Mechanism of Male Germ Cell Apoptosis Induced by Bisphenol-A and Nonylphenol Involving ADAM17 and p38 MAPK Activation"

Article Title: A Mechanism of Male Germ Cell Apoptosis Induced by Bisphenol-A and Nonylphenol Involving ADAM17 and p38 MAPK Activation

Journal: PLoS ONE

doi: 10.1371/journal.pone.0113793

A) Seminiferous tubule cells from rats injected with 50 mg/kg of BPA or NP were isolated as described in the section and incubated with and antibody against the active form of ADAM17. The graph shows that the percentage of cells labeled with the antibody increases in rats treated with BPA or NP, but is reduced when rats were treated in the presence of GW280264X, a pharmacological inhibitor of ADAM17 (GW), or PD169316, a pharmacological inhibitor of p38 MAPK (PD). * p<0.05, n = 3.
Figure Legend Snippet: A) Seminiferous tubule cells from rats injected with 50 mg/kg of BPA or NP were isolated as described in the section and incubated with and antibody against the active form of ADAM17. The graph shows that the percentage of cells labeled with the antibody increases in rats treated with BPA or NP, but is reduced when rats were treated in the presence of GW280264X, a pharmacological inhibitor of ADAM17 (GW), or PD169316, a pharmacological inhibitor of p38 MAPK (PD). * p<0.05, n = 3.

Techniques Used: Injection, Isolation, Incubation, Labeling

A) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of BPA. B) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of NP. C) Intra-testicular application of 5 µM PD169316 reduces the phosphorylation of p38 MAPK 1 or 2 h after in vivo treatment with BPA or NP. D) The pharmacological inhibitor of p38 MAPK (PD169316) prevents the increase of TUNEL-positive cells in testes of 21-day-old rats treated with 50 mg/kg of BPA or NP. * p<0.05, n = 3.
Figure Legend Snippet: A) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of BPA. B) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of NP. C) Intra-testicular application of 5 µM PD169316 reduces the phosphorylation of p38 MAPK 1 or 2 h after in vivo treatment with BPA or NP. D) The pharmacological inhibitor of p38 MAPK (PD169316) prevents the increase of TUNEL-positive cells in testes of 21-day-old rats treated with 50 mg/kg of BPA or NP. * p<0.05, n = 3.

Techniques Used: In Vivo, TUNEL Assay


Structured Review

Santa Cruz Biotechnology total p38 sc 7149 mapks
Total P38 Sc 7149 Mapks, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total p38 sc 7149 mapks/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
total p38 sc 7149 mapks - by Bioz Stars, 2024-05
86/100 stars

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    Santa Cruz Biotechnology total p38 mapk sc 7149
    A) Seminiferous tubule cells from rats injected with 50 mg/kg of BPA or NP were isolated as described in the section and incubated with and antibody against the active form of ADAM17. The graph shows that the percentage of cells labeled with the antibody increases in rats treated with BPA or NP, but is reduced when rats were treated in the presence of GW280264X, a pharmacological inhibitor of ADAM17 (GW), or PD169316, a pharmacological inhibitor of <t>p38</t> <t>MAPK</t> (PD). * p<0.05, n = 3.
    Total P38 Mapk Sc 7149, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total p38 mapk sc 7149/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total p38 mapk sc 7149 - by Bioz Stars, 2024-05
    95/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology total p38 sc 7149 mapks
    A) Seminiferous tubule cells from rats injected with 50 mg/kg of BPA or NP were isolated as described in the section and incubated with and antibody against the active form of ADAM17. The graph shows that the percentage of cells labeled with the antibody increases in rats treated with BPA or NP, but is reduced when rats were treated in the presence of GW280264X, a pharmacological inhibitor of ADAM17 (GW), or PD169316, a pharmacological inhibitor of <t>p38</t> <t>MAPK</t> (PD). * p<0.05, n = 3.
    Total P38 Sc 7149 Mapks, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total p38 sc 7149 mapks/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total p38 sc 7149 mapks - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

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    A) Seminiferous tubule cells from rats injected with 50 mg/kg of BPA or NP were isolated as described in the section and incubated with and antibody against the active form of ADAM17. The graph shows that the percentage of cells labeled with the antibody increases in rats treated with BPA or NP, but is reduced when rats were treated in the presence of GW280264X, a pharmacological inhibitor of ADAM17 (GW), or PD169316, a pharmacological inhibitor of p38 MAPK (PD). * p<0.05, n = 3.

    Journal: PLoS ONE

    Article Title: A Mechanism of Male Germ Cell Apoptosis Induced by Bisphenol-A and Nonylphenol Involving ADAM17 and p38 MAPK Activation

    doi: 10.1371/journal.pone.0113793

    Figure Lengend Snippet: A) Seminiferous tubule cells from rats injected with 50 mg/kg of BPA or NP were isolated as described in the section and incubated with and antibody against the active form of ADAM17. The graph shows that the percentage of cells labeled with the antibody increases in rats treated with BPA or NP, but is reduced when rats were treated in the presence of GW280264X, a pharmacological inhibitor of ADAM17 (GW), or PD169316, a pharmacological inhibitor of p38 MAPK (PD). * p<0.05, n = 3.

    Article Snippet: Rabbit polyclonal antibody against ADAM17 (ab39163), which reacts with an epitope located in the activation site (cysteine switch and furin cleavage site) of ADAM17 – , and p38 (phospho Y182+T 180) were purchased from Abcam (Cambridge, MA, USA), Rabbit polyclonal antibody against PARP-1/2 (sc-7150), clusterin-α (sc-8354), total-p38 MAPK (sc-7149) and mouse monoclonal SCP-3 (sc-74569) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Injection, Isolation, Incubation, Labeling

    A) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of BPA. B) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of NP. C) Intra-testicular application of 5 µM PD169316 reduces the phosphorylation of p38 MAPK 1 or 2 h after in vivo treatment with BPA or NP. D) The pharmacological inhibitor of p38 MAPK (PD169316) prevents the increase of TUNEL-positive cells in testes of 21-day-old rats treated with 50 mg/kg of BPA or NP. * p<0.05, n = 3.

    Journal: PLoS ONE

    Article Title: A Mechanism of Male Germ Cell Apoptosis Induced by Bisphenol-A and Nonylphenol Involving ADAM17 and p38 MAPK Activation

    doi: 10.1371/journal.pone.0113793

    Figure Lengend Snippet: A) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of BPA. B) Time course of p38 MAPK phosphorylation in 21-day-old rats treated with 50 mg/kg of NP. C) Intra-testicular application of 5 µM PD169316 reduces the phosphorylation of p38 MAPK 1 or 2 h after in vivo treatment with BPA or NP. D) The pharmacological inhibitor of p38 MAPK (PD169316) prevents the increase of TUNEL-positive cells in testes of 21-day-old rats treated with 50 mg/kg of BPA or NP. * p<0.05, n = 3.

    Article Snippet: Rabbit polyclonal antibody against ADAM17 (ab39163), which reacts with an epitope located in the activation site (cysteine switch and furin cleavage site) of ADAM17 – , and p38 (phospho Y182+T 180) were purchased from Abcam (Cambridge, MA, USA), Rabbit polyclonal antibody against PARP-1/2 (sc-7150), clusterin-α (sc-8354), total-p38 MAPK (sc-7149) and mouse monoclonal SCP-3 (sc-74569) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: In Vivo, TUNEL Assay