Journal: International Journal of Oncology

Article Title: A disintegrin and metalloprotease 12 contributes to colorectal cancer metastasis by regulating epithelial-mesenchymal transition

doi: 10.3892/ijo.2023.5498

Figure Lengend Snippet: Effects of ADAM12 on oncogenic signaling pathways in the human colorectal cancer cells. Representative western blot images and semi-quantification graphs for the phosphorylation levels of PDK1, GSK-3β and AKT in AV-transfected or AS-transfected DLD1 or SW480 cells. Data are presented as the mean ± SD (n=3). * P<0.05. ADAM12, a disintegrin and metalloprotease 12; PDK1, phosphoinositide-dependent protein kinase 1; GSK-3β, glycogen synthase kinase-3β; EV, empty pcDNA6-myc vector; AV, ADAM12-pcDNA6-myc construct; SS, scrambled siRNA; AS, ADAM12 siRNA; siRNA, small interfering RNA; p-, phosphorylated; T-, total.

Article Snippet: Antibodies against E-cadherin (cat. no. #14472), Snail (cat. no. #3879), vimentin (cat. no. #5741), claudin-1 (cat. no. #4933), integrin α5 (cat. no. #4705), integrin β1 (cat. no. #9699), integrin β3 (cat. no. #13166), phosphorylated (p)-AKT (S473) (cat. no. #4060), p-phosphoinositide-dependent protein kinase 1 (PDK1) (S241) (cat. no. #3438), p-glycogen synthase kinase-3β (GSK-3β) (S9) (cat. no. #9323), total AKT (cat. no. #4691), total PDK1 (cat. no. #3062), total GSK-3β (cat. no. #9832) and Myc-tag (cat. no. #2278) were obtained from Cell Signaling Technology, Inc. Antibodies against β-tubulin (cat. no. sc-9104) and GAPDH (cat. no. sc-25778) were purchased from Santa Cruz Biotechnology, Inc.

Techniques: Western Blot, Transfection, Plasmid Preparation, Construct, Small Interfering RNA

Journal: Cancers

Article Title: 2-Arachidonoylglycerol Modulates CXCL12-Mediated Chemotaxis in Mantle Cell Lymphoma and Chronic Lymphocytic Leukemia

doi: 10.3390/cancers15051585

Figure Lengend Snippet: Activation of signaling pathways. ( A ) JeKo-1, ( B ) Granta519 and ( C ) JVM-2 were incubated for 2 min with CXCL12 (200 ng/mL) or 2-AG (100 nM) or the combination, and the activation of the signaling pathways ERK1/2, Akt and p38 was assessed by Western blotting, normalizing phospho/GAPDH band intensity to total/GAPDH as described in , bars represent an average of the ratio of at least four repeats and error bars are standard error of the mean; paired t -test, * p < 0.05, ** p < 0.01, *** p < 0.001, ns: non-significant, a representative blot for each experiment is shown. Original blot see .

Article Snippet: Antibodies used for Western blotting: anti-phospho ERK1/2 (Thr202/Tyr204), anti-total ERK1/2, anti-phospho p38 (Thr180/Tyr182), anti-total p38, anti-phospho Akt (Ser473) and anti-total Akt were all from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activation Assay, Incubation, Western Blot

Journal: Cancers

Article Title: 2-Arachidonoylglycerol Modulates CXCL12-Mediated Chemotaxis in Mantle Cell Lymphoma and Chronic Lymphocytic Leukemia

doi: 10.3390/cancers15051585

Figure Lengend Snippet: Effects of inhibiting the cannabinoid receptors on Akt and p38 activation. JeKo-1 cells were treated for 20 min with the CB1 antagonist SR141716 (10 nM), or with the CB2 inverse agonist SR1445282 (10 nM), prior to be incubated for 2 min with 2-AG. Activation of the signaling pathways ( A ) Akt and ( B ) p38 was assessed by Western blotting, normalizing phospho/GAPDH band intensity to total/GAPDH as described in , bars represent an average of the ratio of three repeats and error bars are standard error of the mean; 2-AG condition is shown as normalized to incubation with vehicle set as 1, unpaired t -test with Welch’s correction, # p < 0.05.

Article Snippet: Antibodies used for Western blotting: anti-phospho ERK1/2 (Thr202/Tyr204), anti-total ERK1/2, anti-phospho p38 (Thr180/Tyr182), anti-total p38, anti-phospho Akt (Ser473) and anti-total Akt were all from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Activation Assay, Incubation, Western Blot