torula yeast tyrna  (Millipore)


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    Structured Review

    Millipore torula yeast tyrna
    Torula Yeast Tyrna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/torula yeast tyrna/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    torula yeast tyrna - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Nucleic Acid Content in Crustacean Zooplankton: Bridging Metabolic and Stoichiometric Predictions
    Article Snippet: .. Fluorescence measurements were converted into RNA and DNA concentrations by using standard curves for RNA (16S and 23S from Escherichia coli , component C of the RiboGreen Kit) and DNA (calf thymus; Sigma-Aldrich). ..

    Isolation:

    Article Title: H3K27 demethylation by JMJD3 at a poised enhancer of anti-apoptotic gene BCL2 determines ER? ligand dependency
    Article Snippet: .. Total RNA was isolated from MCF7 cells grown in E2-free medium using the GenElute mammalian total RNA miniprep kit (Sigma). .. RNA was reverse transcribed into first-strand cDNA using MMLV reverse transcriptase (Promega).

    Immunoprecipitation:

    Article Title: A novel miR-371a-5p-mediated pathway, leading to BAG3 upregulation in cardiomyocytes in response to epinephrine, is lost in Takotsubo cardiomyopathy
    Article Snippet: .. HEK293 cells were lysed and the RNA-associated proteins were immunoprecipitated (IP) with anti-AGO2 Antibody (Millipore, 03-110). .. The precipitated RNA was retro-transcribed and measured by quantitative real-time PCR (Roche, Universal Probe Library System) by normalization to the non-IP control (Input).

    Article Title: ICF-specific DNMT3B dysfunction interferes with intragenic regulation of mRNA transcription and alternative splicing
    Article Snippet: .. RNA immunoprecipitation (RIP) sequencing and data processing Native and cross-linked RNA immunoprecipitation experiments were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore) and the Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit (Millipore), respectively, according to the manufacturer's instructions. .. Antibodies for RIP assays were anti-DNMT3B (Diagenode), anti-DNMT3A (Abcam) and anti-hnRNP-LL (Aviva).

    Article Title: ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer
    Article Snippet: .. RNA immunoprecipitation RNA immunoprecipitation was performed using Magna RIP RNA-Binding Protein Immunoprecipitation Kit (17-701, Millipore) according to manufacturer’s instructions. .. mRNA stability analysis DLD1 cells were treated with scramble or ILF3 shRNA followed by treatment of Actinomycin D (10 mg/mL) for 0, 3, 6, 9, or 12 h followed by Trizol RNA extraction.

    other:

    Article Title: Refined RIP-seq protocol for epitranscriptome analysis with low input materials
    Article Snippet: We next examined the m6 A MeRIP efficiency by testing different amounts of RNA (32 μg, 12 μg, 6 μg, 2 μg, 1 μg, and 0.5 μg) as starting material using the Millipore antibody.

    Article Title: TGF-β induces miR-100 and miR-125b but blocks let-7a through LIN28B controlling PDAC progression
    Article Snippet: Small nuclear RNA U44 (001094), U47 (001223), or U6 (001973) were used as endogenous controls.

    Sequencing:

    Article Title: ICF-specific DNMT3B dysfunction interferes with intragenic regulation of mRNA transcription and alternative splicing
    Article Snippet: .. RNA immunoprecipitation (RIP) sequencing and data processing Native and cross-linked RNA immunoprecipitation experiments were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore) and the Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit (Millipore), respectively, according to the manufacturer's instructions. .. Antibodies for RIP assays were anti-DNMT3B (Diagenode), anti-DNMT3A (Abcam) and anti-hnRNP-LL (Aviva).

    RNA Binding Assay:

    Article Title: ICF-specific DNMT3B dysfunction interferes with intragenic regulation of mRNA transcription and alternative splicing
    Article Snippet: .. RNA immunoprecipitation (RIP) sequencing and data processing Native and cross-linked RNA immunoprecipitation experiments were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore) and the Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit (Millipore), respectively, according to the manufacturer's instructions. .. Antibodies for RIP assays were anti-DNMT3B (Diagenode), anti-DNMT3A (Abcam) and anti-hnRNP-LL (Aviva).

    Article Title: ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer
    Article Snippet: .. RNA immunoprecipitation RNA immunoprecipitation was performed using Magna RIP RNA-Binding Protein Immunoprecipitation Kit (17-701, Millipore) according to manufacturer’s instructions. .. mRNA stability analysis DLD1 cells were treated with scramble or ILF3 shRNA followed by treatment of Actinomycin D (10 mg/mL) for 0, 3, 6, 9, or 12 h followed by Trizol RNA extraction.

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    Millipore dnp labeled probes
    Co-expression of TAK1/MKK/p38 pathway components. Fluorescent double WISH of mkk6-1/p38-1, pp6/cyld-1, p38-1/cyld-1 , and pp6/mkk6-1 in ( A ) whole worms and ( B ) posterior gut tissue (n = 6–8 worms).( C ) Fluorescent double WISH of pp6/cyld-1 and p38-1/cyld-1 in the anterior mesenchyme (n = 6–8 worms). Identical settings used for posterior gut and anterior mesenchyme for comparison of cells surrounding the gut. Negative controls treated identically but lacking <t>DIG</t> and <t>DNP</t> labeled probes were used to determine non-specific background staining. DOI: http://dx.doi.org/10.7554/eLife.16793.021
    Dnp Labeled Probes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnp labeled probes/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnp labeled probes - by Bioz Stars, 2020-07
    99/100 stars
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    Co-expression of TAK1/MKK/p38 pathway components. Fluorescent double WISH of mkk6-1/p38-1, pp6/cyld-1, p38-1/cyld-1 , and pp6/mkk6-1 in ( A ) whole worms and ( B ) posterior gut tissue (n = 6–8 worms).( C ) Fluorescent double WISH of pp6/cyld-1 and p38-1/cyld-1 in the anterior mesenchyme (n = 6–8 worms). Identical settings used for posterior gut and anterior mesenchyme for comparison of cells surrounding the gut. Negative controls treated identically but lacking DIG and DNP labeled probes were used to determine non-specific background staining. DOI: http://dx.doi.org/10.7554/eLife.16793.021

    Journal: eLife

    Article Title: Pathogenic shifts in endogenous microbiota impede tissue regeneration via distinct activation of TAK1/MKK/p38

    doi: 10.7554/eLife.16793

    Figure Lengend Snippet: Co-expression of TAK1/MKK/p38 pathway components. Fluorescent double WISH of mkk6-1/p38-1, pp6/cyld-1, p38-1/cyld-1 , and pp6/mkk6-1 in ( A ) whole worms and ( B ) posterior gut tissue (n = 6–8 worms).( C ) Fluorescent double WISH of pp6/cyld-1 and p38-1/cyld-1 in the anterior mesenchyme (n = 6–8 worms). Identical settings used for posterior gut and anterior mesenchyme for comparison of cells surrounding the gut. Negative controls treated identically but lacking DIG and DNP labeled probes were used to determine non-specific background staining. DOI: http://dx.doi.org/10.7554/eLife.16793.021

    Article Snippet: DIG-labeled and/or DNP-labeled probes were denatured 5 min at 70°C, and incubated O/N at 56°C in a hybe solution identical to prehybe solution but with Deionized Formamide in place of Formamide, Calbiochem Yeast RNA 0.25 mg/ml in place of Sigma Torula Yeast RNA, and an addition of 5% Dextran Sulfate.

    Techniques: Expressing, Labeling, Staining