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(A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 <t>nM</t> <t>Torin-1</t> (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .
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torin 1 stratech scientific  (Stratech Scientific Ltd)
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(A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 <t>nM</t> <t>Torin-1</t> (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .
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torin 2  (MedChemExpress)
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(A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 <t>nM</t> <t>Torin-1</t> (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .
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torin 1  (MedChemExpress)
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(A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 <t>nM</t> <t>Torin-1</t> (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .
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(A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 <t>nM</t> <t>Torin-1</t> (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .
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torin 1  (Tocris)
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Tocris torin 1
(A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 <t>nM</t> <t>Torin-1</t> (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .
Torin 1, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/torin/pm41968870-199-20-21?v=Tocris
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torin 1 - by Bioz Stars, 2026-07
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(A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 nM Torin-1 (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .

Journal: Life Science Alliance

Article Title: A microRNA generated via lysosomal processing of ribosomal RNA suppresses proinflammatory responses

doi: 10.26508/lsa.202503536

Figure Lengend Snippet: (A, B, C, D) HFF cells were kept in basal conditions (t = 0) or starved (t = 4) in the absence (CTRL) or presence of either (A) 0.3 μM SAR405, (B) 50 nM bafilomycin A (BAF), (C) 20 μM chloroquine (CQ), or (D) cells were not starved but instead treated with 200 nM Torin-1 (depicting results only at t = 4). The expression of miR-21-5p and miR-4488 was quantified by RT–qPCR. Relative quantity (RQ) was calculated by normalizing the relative expression of the miRNA in starved cells to its expression in control cells (t = 0). For statistical analysis procedures, see legend to .

Article Snippet: Chloroquine (C6628; Sigma-Aldrich), bafilomycin A1 (BML-CM110-0100; Enzo), SAR405 (533063; Sigma-Aldrich), and Torin-1 (inh-tor1; InvivoGen) were added to the fresh medium at the onset of the treatment.

Techniques: Expressing, Quantitative RT-PCR, Control

(A) Schematic representation of the pre-miR-4488 sequence, highlighting in blue circles the first 18 nucleotides of the mature miRNA and in red circles the sequence of the primer detecting the pre-miR-4488. (B) Control HFFs or HFFs treated with 200 nM Torin-1 for 4 h were harvested and processed for subcellular fractionation as described in the Materials and Methods section. Cytosolic and lysosome-containing fractions were subjected to RNA extraction, and the relative amounts of pre-miR-4488 were determined by RT–qPCR, using the pre-miR-4488–derived primer.

Journal: Life Science Alliance

Article Title: A microRNA generated via lysosomal processing of ribosomal RNA suppresses proinflammatory responses

doi: 10.26508/lsa.202503536

Figure Lengend Snippet: (A) Schematic representation of the pre-miR-4488 sequence, highlighting in blue circles the first 18 nucleotides of the mature miRNA and in red circles the sequence of the primer detecting the pre-miR-4488. (B) Control HFFs or HFFs treated with 200 nM Torin-1 for 4 h were harvested and processed for subcellular fractionation as described in the Materials and Methods section. Cytosolic and lysosome-containing fractions were subjected to RNA extraction, and the relative amounts of pre-miR-4488 were determined by RT–qPCR, using the pre-miR-4488–derived primer.

Article Snippet: Chloroquine (C6628; Sigma-Aldrich), bafilomycin A1 (BML-CM110-0100; Enzo), SAR405 (533063; Sigma-Aldrich), and Torin-1 (inh-tor1; InvivoGen) were added to the fresh medium at the onset of the treatment.

Techniques: Sequencing, Control, Fractionation, RNA Extraction, Quantitative RT-PCR, Derivative Assay