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Millipore top10
Top10, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/top10/product/Millipore
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
top10 - by Bioz Stars, 2020-02
99/100 stars

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TUNEL Assay:

Article Title: A Hybrid Protein–Polymer Nanoworm Potentiates Apoptosis Better than a Monoclonal Antibody
Article Snippet: Cloning vector (Pet25b(+)), Top10, and Origami B(DE3) were purchased from Novagen (Darmstadt, Germany). .. SYBR safe DNA stain, low and high melting point agarose, annexin V/propidium iodide apoptosis kit, and TUNEL staining kit were purchased from Invitrogen (Grand Island, NY).

Clone Assay:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: Ninety-eight percent of the dxr coding sequence, located between the BanII and BbvCi restriction sites, was removed by restriction enzyme digestion and replaced, via blunt-end ligation cloning, with sequence amplified from the proprietary Gateway destination vector pDEST17 (Life Technologies). .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library.

Article Title: A Hybrid Protein–Polymer Nanoworm Potentiates Apoptosis Better than a Monoclonal Antibody
Article Snippet: .. Cloning vector (Pet25b(+)), Top10, and Origami B(DE3) were purchased from Novagen (Darmstadt, Germany). .. Terrific broth dry powder was purchased from Mo-bio Laboratories (Carlsbad, CA).

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: Fragments were amplified by PCR from chromosomal M. hyopneumoniae strain 232 DNA using Pwo polymerase (Roche Applied Science) and cloned into the pET161/GW/D-TOPO vector (Invitrogen). .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Positron Emission Tomography:

Article Title: Different Antibody Response against the Coxsackievirus A16 VP1 Capsid Protein: Specific or Non-Specific
Article Snippet: .. Vectors, bacterial strains and reagents The prokaryotic expression plasmids pET-32a and pET21b and two E . coli host strains (BL21 (DE3) and Top10) were purchased from Novagen (Darmstadt, Germany). ..

Article Title: Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA
Article Snippet: .. Vectors, bacterial strain and reagents The prokaryotic expression plasmid pET-32a and two E. coli host strains, BL21 (DE3) and Top10, were purchased from Novagen (Darmstadt, Germany). .. HRP-LD5 consists of HRP conjugated to LD5, which is a novel evolved immunoglobulin-binding molecule (NEIBM) with a characteristic structure of alternating B3 domain of Finegoldia magna protein L and D domain of staphylococcal protein A that creates synergistic double binding sites to the VH3 and Vk regions of Fab as well as to IgG Fc .

Article Title: Non-neutralizing Antibody Responses against VP1 in Enterovirus A, B, C and Rhinovirus A species among Infants and Children in Shanghai
Article Snippet: .. The prokaryotic expression plasmid pET-21b and two E. coli host strains, Top10 and BL21 (DE3), were purchased from Novagen (Darmstadt, Germany). .. The NEIBM LD5, a novel evolved immunoglobulin-binding molecule with synergistic double binding sites to the VH3 and VƘ regions of the Fab and to the Fc region of IgG , was conjugated with Horseradish peroxidase (HRP-LD5) and used as a labeled secondary antibody.

Binding Assay:

Article Title: Different Antibody Response against the Coxsackievirus A16 VP1 Capsid Protein: Specific or Non-Specific
Article Snippet: Vectors, bacterial strains and reagents The prokaryotic expression plasmids pET-32a and pET21b and two E . coli host strains (BL21 (DE3) and Top10) were purchased from Novagen (Darmstadt, Germany). .. This configuration creates synergistic double-binding sites for fragment of antigen binding (Fab) VH3 and Vk regions and IgG Fc [ ].

Article Title: Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals
Article Snippet: Vectors, bacterial strains and reagents The prokaryotic expression plasmid pET32a and two E. coli host strains, BL21 (DE3) and Top10, were purchased from Novagen (Darmstadt, Germany). .. HRP-LD5 consists of HRP conjugated to LD5, which is a novel evolved immunoglobulin-binding molecule (NEIBM) with a characteristic structure of an alternating B3 domain of Finegoldia magna protein Land the D domain of staphylococcal protein A that creates synergistic double binding sites to the VH3 and Vk regions of Fab as well as to IgG Fc .

Article Title: Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA
Article Snippet: Vectors, bacterial strain and reagents The prokaryotic expression plasmid pET-32a and two E. coli host strains, BL21 (DE3) and Top10, were purchased from Novagen (Darmstadt, Germany). .. HRP-LD5 consists of HRP conjugated to LD5, which is a novel evolved immunoglobulin-binding molecule (NEIBM) with a characteristic structure of alternating B3 domain of Finegoldia magna protein L and D domain of staphylococcal protein A that creates synergistic double binding sites to the VH3 and Vk regions of Fab as well as to IgG Fc .

Article Title: Non-neutralizing Antibody Responses against VP1 in Enterovirus A, B, C and Rhinovirus A species among Infants and Children in Shanghai
Article Snippet: The prokaryotic expression plasmid pET-21b and two E. coli host strains, Top10 and BL21 (DE3), were purchased from Novagen (Darmstadt, Germany). .. The NEIBM LD5, a novel evolved immunoglobulin-binding molecule with synergistic double binding sites to the VH3 and VƘ regions of the Fab and to the Fc region of IgG , was conjugated with Horseradish peroxidase (HRP-LD5) and used as a labeled secondary antibody.

Plasmid Preparation:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: Ninety-eight percent of the dxr coding sequence, located between the BanII and BbvCi restriction sites, was removed by restriction enzyme digestion and replaced, via blunt-end ligation cloning, with sequence amplified from the proprietary Gateway destination vector pDEST17 (Life Technologies). .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library.

Article Title: Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals
Article Snippet: .. Vectors, bacterial strains and reagents The prokaryotic expression plasmid pET32a and two E. coli host strains, BL21 (DE3) and Top10, were purchased from Novagen (Darmstadt, Germany). .. HRP-LD5 consists of HRP conjugated to LD5, which is a novel evolved immunoglobulin-binding molecule (NEIBM) with a characteristic structure of an alternating B3 domain of Finegoldia magna protein Land the D domain of staphylococcal protein A that creates synergistic double binding sites to the VH3 and Vk regions of Fab as well as to IgG Fc .

Article Title: Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA
Article Snippet: .. Vectors, bacterial strain and reagents The prokaryotic expression plasmid pET-32a and two E. coli host strains, BL21 (DE3) and Top10, were purchased from Novagen (Darmstadt, Germany). .. HRP-LD5 consists of HRP conjugated to LD5, which is a novel evolved immunoglobulin-binding molecule (NEIBM) with a characteristic structure of alternating B3 domain of Finegoldia magna protein L and D domain of staphylococcal protein A that creates synergistic double binding sites to the VH3 and Vk regions of Fab as well as to IgG Fc .

Article Title: A Hybrid Protein–Polymer Nanoworm Potentiates Apoptosis Better than a Monoclonal Antibody
Article Snippet: .. Cloning vector (Pet25b(+)), Top10, and Origami B(DE3) were purchased from Novagen (Darmstadt, Germany). .. Terrific broth dry powder was purchased from Mo-bio Laboratories (Carlsbad, CA).

Article Title: Non-neutralizing Antibody Responses against VP1 in Enterovirus A, B, C and Rhinovirus A species among Infants and Children in Shanghai
Article Snippet: .. The prokaryotic expression plasmid pET-21b and two E. coli host strains, Top10 and BL21 (DE3), were purchased from Novagen (Darmstadt, Germany). .. The NEIBM LD5, a novel evolved immunoglobulin-binding molecule with synergistic double binding sites to the VH3 and VƘ regions of the Fab and to the Fc region of IgG , was conjugated with Horseradish peroxidase (HRP-LD5) and used as a labeled secondary antibody.

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: Fragments were amplified by PCR from chromosomal M. hyopneumoniae strain 232 DNA using Pwo polymerase (Roche Applied Science) and cloned into the pET161/GW/D-TOPO vector (Invitrogen). .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Ligation:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: Ninety-eight percent of the dxr coding sequence, located between the BanII and BbvCi restriction sites, was removed by restriction enzyme digestion and replaced, via blunt-end ligation cloning, with sequence amplified from the proprietary Gateway destination vector pDEST17 (Life Technologies). .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library.

Mutagenesis:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: Paragraph title: Generation of mutant libraries of E. coli dxr and ispD . ... TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library.

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: All in-frame TGA codons were mutated to TGG by using mutated primers or site-directed mutagenesis ( ). .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Synthesized:

Article Title: Steady-State and Pre-Steady-State Kinetic Analysis of Mycobacterium smegmatis Cysteine Ligase (MshC)
Article Snippet: Pfu DNA polymerase was from Stratagene. pET28a(+) and the Escherichia coli strains, Rosetta(DE3)2 and Top10, were obtained from Novagen. .. The oligonucleotide primers were synthesized by Invitrogen.

TA Cloning:

Article Title: Non-neutralizing Antibody Responses against VP1 in Enterovirus A, B, C and Rhinovirus A species among Infants and Children in Shanghai
Article Snippet: Vectors, bacterial strains and reagents The high-efficiency TA cloning vector, pMD18-T, was purchased from TAKARA BIOTECHNOLOGY (DALIAN) CO.,LTD. (Dalian, People’s Republic of China). .. The prokaryotic expression plasmid pET-21b and two E. coli host strains, Top10 and BL21 (DE3), were purchased from Novagen (Darmstadt, Germany).

Cell Culture:

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma). .. Positive colonies were cultured further in LB media containing 100 μg ml−1 ampicillin (Sigma) and plasmids extracted with the QIAprep Spin Miniprep Kit (Qiagen, Netherlands).

Sequencing:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: This sequence had been amplified using the forward primer 5′-CAGGATATCACAAGTTTGTACAAAAAAGCTGAAC-3′ and the reverse primer 5′-GCAGCCTCAGCGGTACCACTTTGTACAAGAAAGCTGAAC-3′. .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library.

Article Title: A Hybrid Protein–Polymer Nanoworm Potentiates Apoptosis Better than a Monoclonal Antibody
Article Snippet: Materials The DNA sequence encoding for anti-CD20 scFv ( ) was purchased from Integrated DNA Technologies (Coralville, IA). .. Cloning vector (Pet25b(+)), Top10, and Origami B(DE3) were purchased from Novagen (Darmstadt, Germany).

IA:

Article Title: A Hybrid Protein–Polymer Nanoworm Potentiates Apoptosis Better than a Monoclonal Antibody
Article Snippet: Materials The DNA sequence encoding for anti-CD20 scFv ( ) was purchased from Integrated DNA Technologies (Coralville, IA). .. Cloning vector (Pet25b(+)), Top10, and Origami B(DE3) were purchased from Novagen (Darmstadt, Germany).

Incubation:

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma). .. Positive colonies were cultured further in LB media containing 100 μg ml−1 ampicillin (Sigma) and plasmids extracted with the QIAprep Spin Miniprep Kit (Qiagen, Netherlands).

Amplification:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: This sequence had been amplified using the forward primer 5′-CAGGATATCACAAGTTTGTACAAAAAAGCTGAAC-3′ and the reverse primer 5′-GCAGCCTCAGCGGTACCACTTTGTACAAGAAAGCTGAAC-3′. .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library.

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: Fragments were amplified by PCR from chromosomal M. hyopneumoniae strain 232 DNA using Pwo polymerase (Roche Applied Science) and cloned into the pET161/GW/D-TOPO vector (Invitrogen). .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Labeling:

Article Title: Non-neutralizing Antibody Responses against VP1 in Enterovirus A, B, C and Rhinovirus A species among Infants and Children in Shanghai
Article Snippet: The prokaryotic expression plasmid pET-21b and two E. coli host strains, Top10 and BL21 (DE3), were purchased from Novagen (Darmstadt, Germany). .. The NEIBM LD5, a novel evolved immunoglobulin-binding molecule with synergistic double binding sites to the VH3 and VƘ regions of the Fab and to the Fc region of IgG , was conjugated with Horseradish peroxidase (HRP-LD5) and used as a labeled secondary antibody.

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: Fragments were labeled F1683 through F5683 and ranged from amino acids 42–308, 306–597, 595–803, 801–1017, and 1018–1194, respectively. .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Expressing:

Article Title: Different Antibody Response against the Coxsackievirus A16 VP1 Capsid Protein: Specific or Non-Specific
Article Snippet: .. Vectors, bacterial strains and reagents The prokaryotic expression plasmids pET-32a and pET21b and two E . coli host strains (BL21 (DE3) and Top10) were purchased from Novagen (Darmstadt, Germany). ..

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library. ..

Article Title: Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals
Article Snippet: .. Vectors, bacterial strains and reagents The prokaryotic expression plasmid pET32a and two E. coli host strains, BL21 (DE3) and Top10, were purchased from Novagen (Darmstadt, Germany). .. HRP-LD5 consists of HRP conjugated to LD5, which is a novel evolved immunoglobulin-binding molecule (NEIBM) with a characteristic structure of an alternating B3 domain of Finegoldia magna protein Land the D domain of staphylococcal protein A that creates synergistic double binding sites to the VH3 and Vk regions of Fab as well as to IgG Fc .

Article Title: Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA
Article Snippet: .. Vectors, bacterial strain and reagents The prokaryotic expression plasmid pET-32a and two E. coli host strains, BL21 (DE3) and Top10, were purchased from Novagen (Darmstadt, Germany). .. HRP-LD5 consists of HRP conjugated to LD5, which is a novel evolved immunoglobulin-binding molecule (NEIBM) with a characteristic structure of alternating B3 domain of Finegoldia magna protein L and D domain of staphylococcal protein A that creates synergistic double binding sites to the VH3 and Vk regions of Fab as well as to IgG Fc .

Article Title: Non-neutralizing Antibody Responses against VP1 in Enterovirus A, B, C and Rhinovirus A species among Infants and Children in Shanghai
Article Snippet: .. The prokaryotic expression plasmid pET-21b and two E. coli host strains, Top10 and BL21 (DE3), were purchased from Novagen (Darmstadt, Germany). .. The NEIBM LD5, a novel evolved immunoglobulin-binding molecule with synergistic double binding sites to the VH3 and VƘ regions of the Fab and to the Fc region of IgG , was conjugated with Horseradish peroxidase (HRP-LD5) and used as a labeled secondary antibody.

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: Paragraph title: Expression of Recombinant Proteins and Creation of Polyclonal Antisera ... The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Polymerase Chain Reaction:

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: Fragments were amplified by PCR from chromosomal M. hyopneumoniae strain 232 DNA using Pwo polymerase (Roche Applied Science) and cloned into the pET161/GW/D-TOPO vector (Invitrogen). .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Staining:

Article Title: A Hybrid Protein–Polymer Nanoworm Potentiates Apoptosis Better than a Monoclonal Antibody
Article Snippet: Cloning vector (Pet25b(+)), Top10, and Origami B(DE3) were purchased from Novagen (Darmstadt, Germany). .. SYBR safe DNA stain, low and high melting point agarose, annexin V/propidium iodide apoptosis kit, and TUNEL staining kit were purchased from Invitrogen (Grand Island, NY).

Transformation Assay:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library. ..

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: .. The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma). .. Positive colonies were cultured further in LB media containing 100 μg ml−1 ampicillin (Sigma) and plasmids extracted with the QIAprep Spin Miniprep Kit (Qiagen, Netherlands).

Recombinant:

Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *
Article Snippet: Paragraph title: Expression of Recombinant Proteins and Creation of Polyclonal Antisera ... The reaction mixture was then transformed into TOP10 chemically competent E. coli and incubated overnight at 37 °C in LB agar containing 100 μg ml−1 ampicillin (Sigma).

Derivative Assay:

Article Title: Resistance to the Antimicrobial Agent Fosmidomycin and an FR900098 Prodrug through Mutations in the Deoxyxylulose Phosphate Reductoisomerase Gene (dxr)
Article Snippet: In order to create a Gateway destination plasmid, with expression under the control of the dxr promoter, a 1,531-bp sequence flanked by the BamHI and EagI sites, and including the entire E. coli -derived sequence (1,455 bp), was cut out of the pCR2.1-derived vector and inserted into pBR322 (New England BioLabs) between the BamHI and EagI restriction sites. .. TOP10 chemically competent cells were transformed with the new expression library and plated on LB agar plates containing 100 μg/ml ampicillin (Sigma-Aldrich) (LB/amp plates), yielding approximately 11,000 colonies in the dxr library and 8,000 colonies in the ispD library.

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