top10 host cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher top10 host cells
    Top10 Host Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 host cells/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    top10 host cells - by Bioz Stars, 2020-04
    86/100 stars

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    pbad/his-b vector

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    Related Articles

    Clone Assay:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen). .. Typical mutant libraries for each FACS screen consisted of approximately 107 independent clones.

    Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
    Article Snippet: Protein characterization in vitro For expression in bacteria, the miRFPs were cloned into pBAD/His-B vector (Life Technologies/Invitrogen). .. LMG194 or TOP10 host cells (Invitrogen) were used for protein expression.

    Amplification:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: For FACS screening, a coding region of GFP variants was PCR amplified as a Bgl II– Eco RI fragment and inserted into a pBAD/His-B vector (Invitrogen). .. After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen).

    In Vitro:

    Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
    Article Snippet: Paragraph title: Protein characterization in vitro ... LMG194 or TOP10 host cells (Invitrogen) were used for protein expression.

    Ligation:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: .. After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen). .. Typical mutant libraries for each FACS screen consisted of approximately 107 independent clones.

    Library Screening:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: Paragraph title: Library Screening ... After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen).

    Mutagenesis:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: Random mutagenesis was performed with a GeneMorph II random mutagenesis kit (Stratagene) under conditions resulting in a mutation frequency up to 16 mutations per 1000 base pairs. .. After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen).

    Incubation:

    Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
    Article Snippet: LMG194 or TOP10 host cells (Invitrogen) were used for protein expression. .. Bacterial cells were incubated overnight at 37 °C in RM minimal medium with ampicillin and kanamycin.

    Expressing:

    Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
    Article Snippet: .. LMG194 or TOP10 host cells (Invitrogen) were used for protein expression. .. A pWA23h plasmid encoding HO from Bradyrhizobium ORS278 (hmuO) under the rhamnose promoter was co-transformed with a pBAD/His-B plasmid encoding a FP.

    Polymerase Chain Reaction:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: For FACS screening, a coding region of GFP variants was PCR amplified as a Bgl II– Eco RI fragment and inserted into a pBAD/His-B vector (Invitrogen). .. After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen).

    FACS:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: For FACS screening, a coding region of GFP variants was PCR amplified as a Bgl II– Eco RI fragment and inserted into a pBAD/His-B vector (Invitrogen). .. After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen).

    Plasmid Preparation:

    Article Title: The First Mutant of the Aequorea victoria Green Fluorescent Protein That Forms a Red Chromophore †
    Article Snippet: .. After ligation into pBAD/His-B vector (Invitrogen), a mixture of the random mutants was electroporated into TOP10 host cells (Invitrogen). .. Typical mutant libraries for each FACS screen consisted of approximately 107 independent clones.

    Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
    Article Snippet: Protein characterization in vitro For expression in bacteria, the miRFPs were cloned into pBAD/His-B vector (Life Technologies/Invitrogen). .. LMG194 or TOP10 host cells (Invitrogen) were used for protein expression.

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    Thermo Fisher top10 escherichia coli
    DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into <t>TOP10</t> <t>Escherichia</t> coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).
    Top10 Escherichia Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 escherichia coli/product/Thermo Fisher
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    top10 escherichia coli - by Bioz Stars, 2020-04
    93/100 stars
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    DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into TOP10 Escherichia coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).

    Journal: Clinical and Experimental Immunology

    Article Title: Tissue‐resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer

    doi: 10.1111/cei.13183

    Figure Lengend Snippet: DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into TOP10 Escherichia coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).

    Article Snippet: Next, the PCR amplicons were TA‐cloned into pCR4‐TOPO vector (ThermoFisher) and transformed into TOP10 Escherichia coli (ThermoFisher) by heat shock, according to the manufacturer’s protocol.

    Techniques: DNA Methylation Assay, Sequencing, Cell Culture, Recombinant, In Vitro, Concentration Assay, Incubation, Flow Cytometry, Cytometry, Expressing, Clone Assay, Plasmid Preparation, Transformation Assay