dylight 594 tomato lectin  (Vector Laboratories)


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    Vector Laboratories dylight 594 tomato lectin
    Dylight 594 Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dylight 594 tomato lectin  (Vector Laboratories)


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    Vector Laboratories dylight 594 tomato lectin
    Dylight 594 Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 594 tomato lectin/product/Vector Laboratories
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    lycopersicon esculentum tomato lectin  (Vector Laboratories)


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    Vector Laboratories lycopersicon esculentum tomato lectin
    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with <t>lectin</t> to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lycopersicon esculentum tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lycopersicon esculentum tomato lectin - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Macroscopic changes in aquaporin-4 underlie blast traumatic brain injury-related impairment in glymphatic function"

    Article Title: Macroscopic changes in aquaporin-4 underlie blast traumatic brain injury-related impairment in glymphatic function

    Journal: Brain

    doi: 10.1093/brain/awae065

    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Figure Legend Snippet: Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.

    Techniques Used: Slice Preparation, Immunostaining, Fluorescence, Expressing, Staining, Generated

    lycopersicon esculentum tomato lectin  (Vector Laboratories)


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    Vector Laboratories lycopersicon esculentum tomato lectin
    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with <t>lectin</t> to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lycopersicon esculentum tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lycopersicon esculentum tomato lectin - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Macroscopic changes in aquaporin-4 underlie blast traumatic brain injury-related impairment in glymphatic function"

    Article Title: Macroscopic changes in aquaporin-4 underlie blast traumatic brain injury-related impairment in glymphatic function

    Journal: Brain

    doi: 10.1093/brain/awae065

    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Figure Legend Snippet: Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.

    Techniques Used: Slice Preparation, Immunostaining, Fluorescence, Expressing, Staining, Generated

    lycopersicon esculentum tomato lectin  (Vector Laboratories)


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    Vector Laboratories lycopersicon esculentum tomato lectin
    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with <t>lectin</t> to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lycopersicon esculentum tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lycopersicon esculentum tomato lectin - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Macroscopic changes in aquaporin-4 underlie blast traumatic brain injury-related impairment in glymphatic function"

    Article Title: Macroscopic changes in aquaporin-4 underlie blast traumatic brain injury-related impairment in glymphatic function

    Journal: Brain

    doi: 10.1093/brain/awae065

    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Figure Legend Snippet: Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.

    Techniques Used: Slice Preparation, Immunostaining, Fluorescence, Expressing, Staining, Generated

    lycopersicon esculentum tomato lectin  (Vector Laboratories)


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    Vector Laboratories lycopersicon esculentum tomato lectin
    Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lycopersicon esculentum tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
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    tomato lectin  (Vector Laboratories)


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    Vector Laboratories tomato lectin
    Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
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    lectin tomato  (Vector Laboratories)


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    Vector Laboratories lectin tomato
    Lectin Tomato, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lectin tomato/product/Vector Laboratories
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    lea tomato lectin  (Vector Laboratories)


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    Vector Laboratories lea tomato lectin
    Lea Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dylight 594 tomato lectin  (Vector Laboratories)


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    Vector Laboratories dylight 594 tomato lectin
    Dylight 594 Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 594 tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
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    Vector Laboratories dylight 594 tomato lectin
    Dylight 594 Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dylight 594 tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
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    Vector Laboratories lycopersicon esculentum tomato lectin
    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with <t>lectin</t> to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lycopersicon esculentum tomato lectin/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
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    lycopersicon esculentum tomato lectin - by Bioz Stars, 2024-06
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    Vector Laboratories tomato lectin
    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with <t>lectin</t> to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with <t>lectin</t> to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
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    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with <t>lectin</t> to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.
    Lea Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.

    Journal: Brain

    Article Title: Macroscopic changes in aquaporin-4 underlie blast traumatic brain injury-related impairment in glymphatic function

    doi: 10.1093/brain/awae065

    Figure Lengend Snippet: Increased AQP4 in the murine brain following mild repetitive blast traumatic brain injury. (A) Representative whole brain slice images of AQP4 immunostaining in mouse brains of Sham group and 7 (7D Blast) and 28 days (28D Blast) post mild repetitive blast traumatic brain injury (mTBI). (B) Representative dorsal cortex images of AQP4 immunostaining in Sham, 7D Blast and 28D Blast mouse brains. Scale bar = 200 μm. Increased AQP4, particularly at the cortical surface, was observed at 28 days post mTBI. (C) Representative images of the regions of interest drawn for regional shell analysis of AQP4 in outer grey, inner grey and white matter and representative dorsal and ventral lines drawn for AQP4 line analysis generate intensity plots of AQP4 across the tissue. (D) AQP4 fluorescence intensity from regional shell analysis showing AQP4 immunoreactivity was significantly increased in the outer grey matter at 28 days post mTBI. Data are mean ± SEM from n = 8–10 per group and were analysed with two-way repeated measure ANOVA followed by Sidak’s post hoc test (*P < 0.05, 28 days versus 7 days post mTBI; *P < 0.05, 28 days post mTBI versus Sham). (E) Averaged fluorescence intensity plots from the dorsal surface through the grey matter (as shown in D) and into the subcortical white matter. Data are mean ± SEM from n = 9–10 mice/group. (F) Averaged fluorescence intensity plots extending from the ventral surface through the grey matter (as shown in D) for n = 9 per group. (G) Quantification of AQP4 expression and localization in the dorsal cortex. AQP4 was co-stained with lectin to label blood vessels, a vessel mask was generated and AQP4 fluorescence intensity was measured inside or outside the vessel mask to quantify perivascular (PV) and non-PV AQP4, respectively. AQP4 ratios were calculated as PV/non-PV. (H) Quantification and localization of AQP4 in the ventral cortex. Data were analysed with two-sided t-test. GM = grey matter; WM = white matter.

    Article Snippet: Following overnight primary incubation, sections were incubated with secondary antibodies donkey anti-rabbit Alexa Fluor 647 (1:500; Invitrogen, Cat. No. #A21207) and DyLight 488-labelled Lycopersicon esculentum (Tomato) lectin (Vector Labs, Cat. No. #DL-1177) or donkey anti-mouse Alexa Fluor 488 (1:250; Invitrogen, Cat. No. #A21202) for 2 h at room temperature.

    Techniques: Slice Preparation, Immunostaining, Fluorescence, Expressing, Staining, Generated