tnfsf11 rankl picokine elisa kit  (Boster Bio)


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    Boster Bio tnfsf11 rankl picokine elisa kit
    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and <t>RANKL.</t> After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of <t>RANKL</t> and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p
    Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnfsf11 rankl picokine elisa kit/product/Boster Bio
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    Price from $9.99 to $1999.99
    tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-08
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    Images

    1) Product Images from "Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling. Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling"

    Article Title: Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling. Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling

    Journal: MedComm

    doi: 10.1002/mco2.131

    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p
    Figure Legend Snippet: Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

    Techniques Used: Mouse Assay, Staining, Isolation, Cell Culture, Incubation, Micro-CT

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    Boster Bio tnfsf11 rankl picokine elisa kit
    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and <t>RANKL.</t> After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of <t>RANKL</t> and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p
    Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-08
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    Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human <t>RANKL</t> ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.
    Human Tnfsf11 Rankl Elisa Kit Picokine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Influence of <t>Syndecan-1</t> deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by <t>ELISA.</t> Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p
    Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

    Journal: MedComm

    Article Title: Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling. Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling

    doi: 10.1002/mco2.131

    Figure Lengend Snippet: Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

    Article Snippet: ATP production was quantified using a CellTiter‐Glo Luminescent Cell Viability Assay Kit (Promega, USA)., Serum levels of OPG and RANKL were measured by mouse OPG ELISA Kit (Boster Biological Technology) and TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) according to the manufacturer's instructions.

    Techniques: Mouse Assay, Staining, Isolation, Cell Culture, Incubation, Micro-CT

    Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Journal: Scientific Reports

    Article Title: Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation

    doi: 10.1038/s41598-021-03484-5

    Figure Lengend Snippet: Characterisation of ameloblastoma cells in 3D culture. Spheroid formation of ( a) AM-1 cells and ( b) AM-3 cells in 3D tumouroids at day 7, red = Phalloidin, blue = DAPI, 20 × Magnification Scale Bar = 50 μm. ( c) Histology H E staining of plexiform patient samples, ( d) 3D AM-1 tumouroids, e follicular patient samples, and (f) 3D AM-3 tumouroids. Similar anastomosing cords and branches were highlighted ( c, d) 10 × Magnification Scale Bar = 100 μm. The formation of the odontogenic islands was highlighted ( e,f) . ( g) CellTiter-Glo 3D Viability-Assay of AM-1, AM-3 and MG-63 cells in 3D tumouroids. Distance of invasion (μm) AM-1, AM-3 and MG-63 cells from the tumour mass to the surrounding stroma within the 3D tumouroids. ( h) Human MMP-2 ELISA. Expression of MMP2. ( i) Human MMP-9 ELISA. Expression of MMP9. ( j) Expression of TNFRSF11A (RANK). Human RANKL ELISA. One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Article Snippet: Total MMP-2 Quantikine ELISA Kit (MMP200, R & D Systems, Abingdon, UK), Human MMP-9 Quantikine ELISA Kit (DMP900, R & D Systems, Abingdon, UK) and Human TNFSF11/RANKL ELISA Kit PicoKine (EK0842, BosterBio, CA, USA) were used based on each of the manufacturer’s protocol.

    Techniques: Staining, Viability Assay, Enzyme-linked Immunosorbent Assay, Expressing

    Introduction of ameloblastoma tumour mass to the 3D bone stroma prior to day 9 completely inhibits bone nodule formation. (a) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 6 inhibits bone nodule formation. Images taken at day 6, 9 and 21, 4 × Magnification, scale bar = 100 μm. ALPL expression. TNFSF11 (RANKL) Expression. ( b) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 9 restricts bone formation by limiting bone nodule number and bone nodule surface area. (c) RT2 Profiler PCR Array was conducted to screen osteogenesis gene of osteoblasts in the 3D bone stroma model and in AM-3 tumour mass introduced 3D bone stroma model at day 8. The AM-3 tumour mass was introduced at day 6 of 3D bone stroma model. Volcano plot shows under-expressed, unchanged and over-expressed genes. The table represents > 3.5-fold under-expressed gene. Horizontal line p-value threshold (0.05). One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Journal: Scientific Reports

    Article Title: Bioengineering the ameloblastoma tumour to study its effect on bone nodule formation

    doi: 10.1038/s41598-021-03484-5

    Figure Lengend Snippet: Introduction of ameloblastoma tumour mass to the 3D bone stroma prior to day 9 completely inhibits bone nodule formation. (a) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 6 inhibits bone nodule formation. Images taken at day 6, 9 and 21, 4 × Magnification, scale bar = 100 μm. ALPL expression. TNFSF11 (RANKL) Expression. ( b) Introduction of AM-1 and AM-3 tumour masses to 3D bone at day 9 restricts bone formation by limiting bone nodule number and bone nodule surface area. (c) RT2 Profiler PCR Array was conducted to screen osteogenesis gene of osteoblasts in the 3D bone stroma model and in AM-3 tumour mass introduced 3D bone stroma model at day 8. The AM-3 tumour mass was introduced at day 6 of 3D bone stroma model. Volcano plot shows under-expressed, unchanged and over-expressed genes. The table represents > 3.5-fold under-expressed gene. Horizontal line p-value threshold (0.05). One-Way ANOVA, Dunnet’s Post Hoc; p-values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = ****.

    Article Snippet: Total MMP-2 Quantikine ELISA Kit (MMP200, R & D Systems, Abingdon, UK), Human MMP-9 Quantikine ELISA Kit (DMP900, R & D Systems, Abingdon, UK) and Human TNFSF11/RANKL ELISA Kit PicoKine (EK0842, BosterBio, CA, USA) were used based on each of the manufacturer’s protocol.

    Techniques: Expressing, Polymerase Chain Reaction

    Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p

    Journal: bioRxiv

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1101/852590

    Figure Lengend Snippet: Influence of Syndecan-1 deficiency on serum concentration of RANKL and OPG and bone structure in mice during aging. A, B, C : Serum was collected from female mice (WT (all), Syndecan-1 KO (only in B , C )) of 4, 12 and 18 month of age and concentration of Syndecan-1 ( A ), RANKL ( B ) and OPG ( C ) was determined by ELISA. Data are displayed as mean ± SD, Kruskal-Wallis test with Dunn’s post hoc test, n =9-13 per group. *p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Concentration Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM+ medium (day 7). A : mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. B, C : The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann-Whitney-U test, *p

    Journal: bioRxiv

    Article Title: The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis

    doi: 10.1101/852590

    Figure Lengend Snippet: Syndecan-1, RANKL and OPG expression was analysed after stimulation of osteoblastic cells with DM or DM+ medium (day 7). A : mRNA expression of Syndecan-1 (in WT cells) was determined by real time PCR. B, C : The concentration of soluble Syndecan-1 protein in the supernatant ( B ) and cell layer ( C ) of cells (WT) was analysed by ELISA. Experiments were performed in triplicates. Mann-Whitney-U test, *p

    Article Snippet: The concentration of Syndecan-1, RANKL and OPG was determined using an ELISA Kit (Syndecan-1: Boster; RANKL/OPG: R & D).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY