tnfsf11 rankl picokine elisa kit  (Boster Bio)


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    Boster Bio tnfsf11 rankl picokine elisa kit
    Adipocytes are the principal source for <t>RANKL</t> after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P
    Tnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnfsf11 rankl picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-12
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    1) Product Images from "IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism"

    Article Title: IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism

    Journal: Redox Biology

    doi: 10.1016/j.redox.2022.102373

    Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P
    Figure Legend Snippet: Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Techniques Used: Expressing, Staining, Mouse Assay, Quantitative RT-PCR, Micro-CT

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    Boster Bio humantnfsf11 rankl picokine elisa kit
    Adipocytes are the principal source for <t>RANKL</t> after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P
    Humantnfsf11 Rankl Picokine Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/humantnfsf11 rankl picokine elisa kit/product/Boster Bio
    Average 94 stars, based on 1 article reviews
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    humantnfsf11 rankl picokine elisa kit - by Bioz Stars, 2022-12
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    Boster Bio elisa kits
    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and <t>RANKL.</t> After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of <t>RANKL</t> and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p
    Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/Boster Bio
    Average 94 stars, based on 1 article reviews
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    Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Journal: Redox Biology

    Article Title: IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism

    doi: 10.1016/j.redox.2022.102373

    Figure Lengend Snippet: Adipocytes are the principal source for RANKL after loss of IFT20 in MSCs, and IFT20 deficiency in adipocytes reverses bone phenotype by reducing RANKL expression . (A) Representative TRAP-stained image of femurs from 1-month-old Prx1-Cre; IFT20 f/f mice and controls. Scale bar, 200 μm. N = 5. High magnification image of red box area was at right. Scale bars, 100 μm. The red arrow indicates adipocytes that are found in close vicinity to TRAP positive osteoclasts. (B, C) qRT-PCR analysis of RANKL in whole bone marrow (B) and bone marrow adipose tissue (C). (D) The serum level of RANKL from 1-month-old Prx1-Cre; IFT20 f/f mice were significantly increased compared to age-mated controls. (E) The serum RANKL/OPG ratio was identified in 1-month-old Prx1-Cre; IFT20 f/f mice and controls as indicated. (F) Representative micro-CT image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 1 mm. N = 5. (G) Quantitative BMD measurements of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. (H) Representative H E-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bars, 200 μm. N = 5. (I) Representative TRAP-stained image of femurs from 1-month-old Adipoq-Cre; IFT20 f/f mice and controls. Scale bar, 100 μm. The corresponding quantitative analysis of TRAP staining were at right. (J, K) qRT-PCR analysis of RANKL in whole bone marrow (J) and bone marrow adipose tissue (K). (L) The serum levels of RANKL from 1-month-old Adipoq-Cre; IFT20 f/f mice were significantly decreased compared to controls. Error bars were the means ± SEM from three independent experiments. * P

    Article Snippet: Serum levels of OCN, OPG, RANKL and insulin were measured by mouse Osteocalcin ELISA Kit (BioVision), OPG ELISA Kit (Boster Biological Technology), TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) and Ultra-Sensitive Insulin ELISA Kit (Crystal Chem), respectively, according to the manufacturer's instructions.

    Techniques: Expressing, Staining, Mouse Assay, Quantitative RT-PCR, Micro-CT

    Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

    Journal: MedComm

    Article Title: Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling. Deletion of Trp53 and Rb1 in Ctsk‐expressing cells drives osteosarcoma progression by activating glucose metabolism and YAP signaling

    doi: 10.1002/mco2.131

    Figure Lengend Snippet: Osteosarcoma in Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice originates from mesenchymal cells. (A) Representative TRAP‐stained images. Monocytes were isolated from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls, and cultured in the presence of M‐CSF and RANKL. After incubation of 5 days, the cells were stained for TRAP. Scale bar: 100 μm. (B) The corresponding quantitative analysis of (A) was performed. (C and D) Pit formation was analyzed and quantified as indicated. Scale bar: 100 μm. (E and F) Serum levels of RANKL and OPG shown at 4‐month‐old mice. (G) Representative X‐ray images of Lysm‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 6 months. (H) Representative H E‐stained images of cortical bone from Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls as indicated time point. Scale bar: 100 μm. (I) Representative micro‐CT images of cortical bone from the Ctsk‐Cre;Trp53 f/f /Rb1 f/f mice and controls at age of 7 months. The red arrow indicates osteosarcoma in the bone. Scale bar: 1 mm. Error bars are the means ± SEM from three independent experiments. ** p

    Article Snippet: ATP production was quantified using a CellTiter‐Glo Luminescent Cell Viability Assay Kit (Promega, USA)., Serum levels of OPG and RANKL were measured by mouse OPG ELISA Kit (Boster Biological Technology) and TNFSF11/RANKL PicoKine ELISA Kit (Boster Biological Technology) according to the manufacturer's instructions.

    Techniques: Mouse Assay, Staining, Isolation, Cell Culture, Incubation, Micro-CT