tnfa  (Sino Biological)


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  • 91
    Name:
    TNF alpha TNFA TNFSF2 TNFSF2 Antibody Rabbit PAb Antigen Affinity Purified
    Description:
    Produced in rabbits immunized with purified recombinant Human TNF alpha TNFa TNFSF2 rh TNF alpha TNFa TNFSF2 Catalog 10602 HNAE NP 000585 2 Val77 Leu233 TNF alpha TNFa TNFSF2 specific IgG was purified by Human TNF alpha TNFa TNFSF2 affinity chromatography
    Catalog Number:
    10602-RP02
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    Human
    Applications:
    WB,ELISA,IP
    Immunogen:
    Recombinant Human TNF-alpha / TNFa / TNFSF2 protein (Catalog#10602-HNAE)
    Antibody Type:
    PAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological tnfa
    <t>FoxM1</t> regulates ADAM-17 expression promoting <t>TNFa</t> expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P
    Produced in rabbits immunized with purified recombinant Human TNF alpha TNFa TNFSF2 rh TNF alpha TNFa TNFSF2 Catalog 10602 HNAE NP 000585 2 Val77 Leu233 TNF alpha TNFa TNFSF2 specific IgG was purified by Human TNF alpha TNFa TNFSF2 affinity chromatography
    https://www.bioz.com/result/tnfa/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfa - by Bioz Stars, 2021-06
    91/100 stars

    Images

    1) Product Images from "ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1"

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4294-9

    FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P
    Figure Legend Snippet: FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Techniques Used: Expressing, Stable Transfection, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, shRNA, Over Expression

    2) Product Images from "ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1"

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4294-9

    FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P
    Figure Legend Snippet: FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Techniques Used: Expressing, Stable Transfection, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, shRNA, Over Expression

    Related Articles

    Western Blot:

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1
    Article Snippet: .. Western blot analysis was used to detect the protein levels of FoxM1 (K19, Santa Cruz Biotechnology), ADAM-17 (H300, Santa Cruz Biotechnology), and TNFa (10602-RP02, Sino Biological, Inc.) according to standard procedures [ – ]. .. The levels of TNFa were measured in cell-free supernatants of stably transfected FoxM1-overexpressing RBE cells, FoxM1-shRNA QBC939 cells, and the control cells.

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1
    Article Snippet: .. Western blot analysisWestern blot analysis was used to detect the protein levels of FoxM1 (K19, Santa Cruz Biotechnology), ADAM-17 (H300, Santa Cruz Biotechnology), and TNFa (10602-RP02, Sino Biological, Inc.) according to standard procedures [ – ]. .. Measurement of TNFαreleaseThe levels of TNFa were measured in cell-free supernatants of stably transfected FoxM1-overexpressing RBE cells, FoxM1-shRNA QBC939 cells, and the control cells.

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  • 92
    Sino Biological tnf α
    Serine/threonine kinase 24 (Stk24) deficiency inhibits interleukin 17 (IL-17)-induced inflammation in vivo . (A,B) Wild-type (WT) ( n = 5) and Stk24 h/h ( n = 5) mice were treated by intraperitoneal injection of PBS or IL-17 (0.5 µg in 200 µl PBS) for 24 h. The infiltration of total cells or neutrophils (Gr-1 + CD11b + ) into peritoneal lavage fluid was assessed by FACS (A) , and peritoneal mesothelial cells were isolated to determine IL-6, CXCL2, CCL20 , and <t>TNF-α</t> mRNA expression (B) . (C–G) Infiltration of total cells or neutrophils (Gr-1 + CD11b + ) into the bronchoalveolar lavage fluid (BALF) of WT and Stk24 h/h mice ( n = 5 per group) following intranasal injection of PBS (30 µl) or IL-17 (2 µg in 30 µl PBS), assessed 24 h after injection (C) . (D) Histology of lung tissue from mice treated as described in panel (C) . (E) Enumeration of the inflammatory infiltrates in lung tissue of panel (D) . (F) Lung tissue was examined for IL-6, CXCL2, CCL20 , and TNF-α mRNA expression. (G) ELISA of IL-6, CCL20, and TNF-α in the BALF isolated from mice treated as described in panel (C) (* P
    Tnf α, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α - by Bioz Stars, 2021-06
    92/100 stars
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    91
    Sino Biological tnfa
    <t>FoxM1</t> regulates ADAM-17 expression promoting <t>TNFa</t> expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P
    Tnfa, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnfa/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfa - by Bioz Stars, 2021-06
    91/100 stars
      Buy from Supplier

    93
    Sino Biological tumor necrosis factor alpha tnf α
    AMMSCs in PL showed superior immunosuppression effects with UCMSCs. When primed by IFN-γ and/or <t>TNF-α</t> induction, PGE 2 ( a ) and TGF-β1 ( b ) expression were analyzed. IDO activity ( c ) was evaluated by kynurenine levels. ( d ) UCMSCs suppressed allogeneic lymphocyte proliferation. Bars represented means ± SD, n = 5; * P
    Tumor Necrosis Factor Alpha Tnf α, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tumor necrosis factor alpha tnf α/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tumor necrosis factor alpha tnf α - by Bioz Stars, 2021-06
    93/100 stars
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    N/A
    A DNA sequence encoding the cynomolgus TNF NP 001272206 1 Val 77 Leu233 was expressed and purified with an initial Met
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    Image Search Results


    Serine/threonine kinase 24 (Stk24) deficiency inhibits interleukin 17 (IL-17)-induced inflammation in vivo . (A,B) Wild-type (WT) ( n = 5) and Stk24 h/h ( n = 5) mice were treated by intraperitoneal injection of PBS or IL-17 (0.5 µg in 200 µl PBS) for 24 h. The infiltration of total cells or neutrophils (Gr-1 + CD11b + ) into peritoneal lavage fluid was assessed by FACS (A) , and peritoneal mesothelial cells were isolated to determine IL-6, CXCL2, CCL20 , and TNF-α mRNA expression (B) . (C–G) Infiltration of total cells or neutrophils (Gr-1 + CD11b + ) into the bronchoalveolar lavage fluid (BALF) of WT and Stk24 h/h mice ( n = 5 per group) following intranasal injection of PBS (30 µl) or IL-17 (2 µg in 30 µl PBS), assessed 24 h after injection (C) . (D) Histology of lung tissue from mice treated as described in panel (C) . (E) Enumeration of the inflammatory infiltrates in lung tissue of panel (D) . (F) Lung tissue was examined for IL-6, CXCL2, CCL20 , and TNF-α mRNA expression. (G) ELISA of IL-6, CCL20, and TNF-α in the BALF isolated from mice treated as described in panel (C) (* P

    Journal: Frontiers in Immunology

    Article Title: Protein Kinase Serine/Threonine Kinase 24 Positively Regulates Interleukin 17-Induced Inflammation by Promoting IKK Complex Activation

    doi: 10.3389/fimmu.2018.00921

    Figure Lengend Snippet: Serine/threonine kinase 24 (Stk24) deficiency inhibits interleukin 17 (IL-17)-induced inflammation in vivo . (A,B) Wild-type (WT) ( n = 5) and Stk24 h/h ( n = 5) mice were treated by intraperitoneal injection of PBS or IL-17 (0.5 µg in 200 µl PBS) for 24 h. The infiltration of total cells or neutrophils (Gr-1 + CD11b + ) into peritoneal lavage fluid was assessed by FACS (A) , and peritoneal mesothelial cells were isolated to determine IL-6, CXCL2, CCL20 , and TNF-α mRNA expression (B) . (C–G) Infiltration of total cells or neutrophils (Gr-1 + CD11b + ) into the bronchoalveolar lavage fluid (BALF) of WT and Stk24 h/h mice ( n = 5 per group) following intranasal injection of PBS (30 µl) or IL-17 (2 µg in 30 µl PBS), assessed 24 h after injection (C) . (D) Histology of lung tissue from mice treated as described in panel (C) . (E) Enumeration of the inflammatory infiltrates in lung tissue of panel (D) . (F) Lung tissue was examined for IL-6, CXCL2, CCL20 , and TNF-α mRNA expression. (G) ELISA of IL-6, CCL20, and TNF-α in the BALF isolated from mice treated as described in panel (C) (* P

    Article Snippet: Reagents and AntibodiesRecombinant IL-17A (mouse and human) were purchased from PeproTech (PeproTech, Rocky Hill, NJ, USA); human IL-17F (11855-HNAE), IL-1β, and TNF-α were purchased from Sino Biological Inc.; Primary antibodies against P-P65 (3033S), P-P38 (4511S), P-ERK1/2 (4370S), P-JNK (9251S), P-IκBα (2859), P-IKKα/β (2697), P-TAK1 (4508), IκBα (4814), P65 (8242), P38 (8690), and ERK1/2 (4695) were purchased from Cell Signaling Technology.

    Techniques: In Vivo, Mouse Assay, Injection, FACS, Isolation, Expressing, Enzyme-linked Immunosorbent Assay

    FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Journal: BMC Cancer

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1

    doi: 10.1186/s12885-018-4294-9

    Figure Lengend Snippet: FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Article Snippet: Western blot analysis was used to detect the protein levels of FoxM1 (K19, Santa Cruz Biotechnology), ADAM-17 (H300, Santa Cruz Biotechnology), and TNFa (10602-RP02, Sino Biological, Inc.) according to standard procedures [ – ].

    Techniques: Expressing, Stable Transfection, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, shRNA, Over Expression

    FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Journal: BMC Cancer

    Article Title: ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1

    doi: 10.1186/s12885-018-4294-9

    Figure Lengend Snippet: FoxM1 regulates ADAM-17 expression promoting TNFa expression and cleavage. a RBE cells were stably transfected with FoxM1 and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). b QBC 939 cells were stably transfected with FoxM1-shRNA and protein levels of FoxM1, ADAM-17, and TNFa were detected in the whole cell lysates by Western blotting (upper) and TNFa was detected by ELISA in the supernatants (lower). OE: overexpression. * P

    Article Snippet: Western blot analysis Western blot analysis was used to detect the protein levels of FoxM1 (K19, Santa Cruz Biotechnology), ADAM-17 (H300, Santa Cruz Biotechnology), and TNFa (10602-RP02, Sino Biological, Inc.) according to standard procedures [ – ].

    Techniques: Expressing, Stable Transfection, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, shRNA, Over Expression

    AMMSCs in PL showed superior immunosuppression effects with UCMSCs. When primed by IFN-γ and/or TNF-α induction, PGE 2 ( a ) and TGF-β1 ( b ) expression were analyzed. IDO activity ( c ) was evaluated by kynurenine levels. ( d ) UCMSCs suppressed allogeneic lymphocyte proliferation. Bars represented means ± SD, n = 5; * P

    Journal: BMC Cell Biology

    Article Title: Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditions

    doi: 10.1186/s12860-018-0178-8

    Figure Lengend Snippet: AMMSCs in PL showed superior immunosuppression effects with UCMSCs. When primed by IFN-γ and/or TNF-α induction, PGE 2 ( a ) and TGF-β1 ( b ) expression were analyzed. IDO activity ( c ) was evaluated by kynurenine levels. ( d ) UCMSCs suppressed allogeneic lymphocyte proliferation. Bars represented means ± SD, n = 5; * P

    Article Snippet: Both UCMSCs and AMMSCs cultured in PL-supplemented media at passage 5 were treated with 15 ng/ml interferon-gamma (IFN-γ) and/or 15 ng/ml tumor necrosis factor-alpha (TNF-α), for 48 h. Prostaglandin E2 (PGE2 ) and TGF-β1 concentrations in conditioned medium were quantified using ELISA (Sino Biological Inc., Beijing, China) according to the manufacturer’s instructions.

    Techniques: Expressing, Activity Assay